scholarly journals Affinity between TBC1D4 (AS160) phosphotyrosine-binding domain and insulin-regulated aminopeptidase cytoplasmic domain measured by isothermal titration calorimetry

BMB Reports ◽  
2012 ◽  
Vol 45 (6) ◽  
pp. 360-364 ◽  
Author(s):  
Sang-Youn Park ◽  
Keon-Young Kim ◽  
Sun-Min Kim ◽  
Young-Seok Yu
Keyword(s):  
Isothermal Titration Calorimetry ◽  
Cytoplasmic Domain ◽  
Binding Domain ◽  
Titration Calorimetry ◽  
Phosphotyrosine Binding Domain

scholarly journals The receptor-binding domain: aptamers (SARS-CoV-2) binding investigation by isothermal titration calorimetry

2021 ◽  
pp. 74-75
Author(s):  
V Dominguez Arca ◽  
Keyword(s):  
Computational Modeling ◽  
Isothermal Titration Calorimetry ◽  
Receptor Binding ◽  
Binding Domain ◽  
Computational Method ◽  
Titration Calorimetry ◽  
Receptor Binding Domain ◽  
Optimal Conditions ◽  
Gold Substrates ◽  
And Calorimetry

The aim of the research. To optimize the calorimetry method, which allows to study the binding of the receptor-binding domain (RBD) protein of the coronavirus COVID-19 to aptamer 27 obtained using a computational method. Material and methods. Computational modeling and calorimetry methods were used to optimize the method. Results. Th e results showed that the calorimetry method makes it possible to assess the binding of the RBD protein to the aptamer. Conclusion. Th ese experiments will be run as soon as will be possible to determine the optimal conditions to functionalize gold substrates with the previously thiolated aptamers.

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