scholarly journals Cloning and characterization of polyA-RNA transcripts encoded by activated B1-like retrotransposons in mouse erythroleukemia MEL cells exposed to methylation inhibitors

BMB Reports ◽  
2012 ◽  
Vol 45 (2) ◽  
pp. 126-131 ◽  
Author(s):  
Sotirios S. Tezias ◽  
Asterios S. Tsiftsoglou ◽  
Elsa P. Amanatiadou ◽  
Ioannis S. Vizirianakis
Keyword(s):  
Rna Transcripts ◽  
Mouse Erythroleukemia

Infectious RNA transcripts from ross river virus cDNA clones and the construction and characterization of defined chimeras with sindbis virus

Virology ◽  
1991 ◽  
Vol 182 (2) ◽  
pp. 430-441 ◽  
Author(s):  
Richard J. Kuhn ◽  
Hubert G.M. Niesters ◽  
Zhang Hong ◽  
James H. Strauss
Keyword(s):  
Sindbis Virus ◽  
Ross River Virus ◽  
Cdna Clones ◽  
Rna Transcripts ◽  
Virus Cdna

scholarly journals Characterization of productive and sterile transcripts from the immunoglobulin heavy-chain locus: processing of micron and muS mRNA.

1983 ◽  
Vol 3 (7) ◽  
pp. 1317-1332 ◽  
Author(s):  
K J Nelson ◽  
J Haimovich ◽  
R P Perry
Keyword(s):  
Heavy Chain ◽  
Plasma Cells ◽  
Alternative Polyadenylation ◽  
Initiation Site ◽  
Rna Transcripts ◽  
Chain Locus ◽  
Heavy Chain Locus ◽  
Processing Pathways ◽  
Polyadenylation Sites

An analysis of the sizes and sequence content of nuclear RNA transcripts of the heavy-chain locus in two B-cell lymphomas, 70Z/3 and 38C-13, and in selected hybridoma derivatives of 38C has led to the identification of two distinct precursors of the mRNAs encoding the membrane and secretory forms of mu chain. These precursors, termed Pm1 and Ps1, extend from a common 5' terminus (presumably the cap site) to alternative polyadenylation sites located 3' of the membrane and secretory tailpieces, Pm1 and Ps1 are present in similar amounts in lymphomas, indicating roughly equivalent usage of the two polyadenylation sites, whereas Ps1 much greater than Pm1 in hybridomas, indicating that mature plasma cells produce a trans-acting factor which enhances cleavage at the proximal (muS) site. The lymphomas also synthesize several nonproductive or sterile mu (Smu) transcripts from the second H allele. One class of sterile mu transcripts appears to be initiated about 1 kilobase downstream from the JH4 element. In 70Z, in which the nonproductive H allele has undergone a D1J2 fusion, another initiation site was located about 0.3 kilobase upstream of the D1 element. The sterile mu transcripts exhibit the same regulated termination at alternative polyadenylation sites as the mu mRNA precursors, although their rate of production is not necessarily coupled to that of the productive allele. This analysis has also defined probable processing pathways for productive and sterile components in which there is a 5' leads to 3' order for the excision of the large introns.

scholarly journals Tiazofurin induction of mouse erythroleukemia cell hemoglobin production in the absence of commitment or changes in protooncogene expression

Blood ◽  
1989 ◽  
Vol 73 (2) ◽  
pp. 431-434 ◽  
Author(s):  
ML Sherman ◽  
TD Shafman ◽  
MS Colman ◽  
DW Kufe
Keyword(s):  
Cell Differentiation ◽  
Dependent Manner ◽  
Beta Globin ◽  
Inosine Monophosphate Dehydrogenase ◽  
Concentration Dependent Manner ◽  
Nucleotide Biosynthesis ◽  
Rna Transcripts ◽  
Erythroleukemia Cell ◽  
Hemoglobin Production ◽  
Mouse Erythroleukemia

Abstract Tiazofurin (2-beta-D-ribofuranosylthiazole-4-carboxamide, NSC 286193), is a synthetic nucleoside inhibitor of inosine monophosphate dehydrogenase and blocks guanine nucleotide biosynthesis. In the present study, we examined the effects of tiazofurin on mouse erythroleukemia (MEL) cell differentiation and protooncogene expression. Tiazofurin induced hemoglobin production in MEL cells in a concentration-dependent manner, as measured by an increase in benzidine staining. Northern blot analysis of MEL cells treated with 7 mumol/L tiazofurin demonstrated accumulation of both alpha- and beta-globin RNA transcripts. This induction of differentiation was blocked by the presence of exogenous guanosine (100 mumol/L). In contrast to the down- regulation of c-myc and c-myb RNA in MEL cells induced by dimethyl sulfoxide (DMSO) or hexamethylene bisacetamide (HMBA), there was no detectable change in levels of these transcripts after tiazofurin treatment. Furthermore, MEL cells induced by tiazofurin did not commit to terminal differentiation. These results suggest a role for guanine nucleotides, at least in part, in the regulation of MEL cell differentiation.

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