scholarly journals Importance of Interactions of the .ALPHA.-Helices in the Catalytic Domain N- and C-Terminals of the Family 10 Xylanase from Streptomyces olivaceoviridis E-86 to the Stability of the Enzyme

2009 ◽  
Vol 56 (3) ◽  
pp. 165-171 ◽  
Author(s):  
Satoshi Kaneko ◽  
Shigeyasu Ito ◽  
Zui Fujimoto ◽  
Atsushi Kuno ◽  
Hitomi Ichinose ◽  
...  
Keyword(s):  
Catalytic Domain ◽  
Alpha Helices ◽  
The Family ◽  
Streptomyces Olivaceoviridis ◽  
Family 10 Xylanase ◽  
The Stability

In Silico and in Vitro Evaluation of Deamidation Effects on the Stability of the Fusion Toxin DAB389IL-2

2019 ◽  
Vol 16 (4) ◽  
pp. 307-313 ◽  
Author(s):  
Nasrin Zarkar ◽  
Mohammad Ali Nasiri Khalili ◽  
Fathollah Ahmadpour ◽  
Sirus Khodadadi ◽  
Mehdi Zeinoddini
Keyword(s):  
In Silico ◽  
Catalytic Domain ◽  
Md Simulations ◽  
Special Importance ◽  
Native Form ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Pharmaceutical Protein ◽  
The Stability

Background: DAB389IL-2 (Denileukin diftitox) as an immunotoxin is a targeted pharmaceutical protein and is the first immunotoxin approved by FDA. It is used for the treatment of various kinds of cancer such as CTCL lymphoma, melanoma, and Leukemia but among all of these, treatment of CTCL has special importance. DAB389IL-2 consists of two distinct parts; the catalytic domain of Diphtheria Toxin (DT) that genetically fused to the whole IL-2. Deamidation is the most important reaction for chemical instability of proteins occurs during manufacture and storage. Deamidation of asparagine residues occurs at a higher rate than glutamine residues. The structure of proteins, temperature and pH are the most important factors that influence the rate of deamidation. Methods: Since there is not any information about deamidation of DAB389IL-2, we studied in silico deamidation by Molecular Dynamic (MD) simulations using GROMACS software. The 3D model of fusion protein DAB389IL-2 was used as a template for deamidation. Then, the stability of deamidated and native form of the drug was calculated. Results: The results of MD simulations were showed that the deamidated form of DAB389IL-2 is more unstable than the normal form. Also, deamidation was carried by incubating DAB389IL-2, 0.3 mg/ml in ammonium hydrogen carbonate for 24 h at 37o C in order to in vitro experiment. Conclusion: The results of in vitro experiment were confirmed outcomes of in silico study. In silico and in vitro experiments were demonstrated that DAB389IL-2 is unstable in deamidated form.

Use of an Ambulatory Infusion Pump in a 12-Year-Old with Salmonella Osteomyelitis

DICP ◽  
1989 ◽  
Vol 23 (5) ◽  
pp. 379-381
Author(s):  
Allen J. Vaida ◽  
Chantel J. Mattiucci ◽  
Steven A. Shapiro ◽  
Linda A. Gusenko ◽  
Anna M. King
Keyword(s):  
Cost Saving ◽  
Antibiotic Therapy ◽  
Infusion Pump ◽  
Medication Administration ◽  
Sterile Water ◽  
Home Therapy ◽  
Parenteral Antibiotic ◽  
The Family ◽  
The Stability ◽  
Salmonella Osteomyelitis

A 12-year-old girl with sickle cell hemoglobinopathy presented with a Salmonella osteomyelitis of her right humerus requiring six weeks of parenteral antibiotic therapy. Home therapy was evaluated. Due to the frequency of the medication administration (every six hours) and the apprehension of the family members, a Pharmacia-Deltec CADD-VT Infusion Pump was chosen for drug administration. Based on the stability of ampicillin, 1.3 g q6h was administered to provide a minimum of 1 g for the last dose of a 24-hour cycle. Ampicillin 6 g contained in 100 mL of sterile water for injection provided a 60 mg/mL solution with an osmolarity of 347 mOsmol. The pump was programmed to deliver 22 mL of solution over one hour, every six hours. A keep-vein-open rate of 0.2 mL/h maintained line patency. A 100 mL cassette of solution prepared daily was replaced on the pump by a home therapy nurse each morning. At the end of six weeks of therapy, the osteomyelitis was eradicated. We found the use of an ambulatory infusion pump an effective, convenient, and cost-saving method of treatment for our patient.

Separating the Contributions in Localization Methods: A Technique to Enhance Identification of Damping Related Parameters

1999 ◽  
Author(s):  
Hervé Algrain ◽  
Calogero Conti ◽  
Pierre Dehombreux
Keyword(s):  
Finite Element ◽  
Model Updating ◽  
Element Model ◽  
Dynamic Responses ◽  
Finite Element Model Updating ◽  
Global Localization ◽  
Localization Method ◽  
The Family ◽  
Family Based ◽  
The Stability

Abstract Finite Element Model Updating has for objective to increase the correlation between the experimental dynamic responses of a structure and the predictions from a model. Among different initial choices, these procedures need to establish a set of representative parameters to be updated in which some are in real error and some are not. It is therefore important to select the correct properties that have to be updated to ensure that no marginal corrections are introduced. In this paper the standard localization criteria are presented and a technique to separate the global localization criteria in family-based criteria for damped structures is introduced. The methods are analyzed and applied to both numerical and experimental examples; a clear enhancement of the results is noticed using the family-based criteria. A simple way to qualify the stability of a localization method to noise is presented.

The yeast UME5 gene regulates the stability of meiotic mRNAs in response to glucose

1994 ◽  
Vol 14 (5) ◽  
pp. 3446-3458
Author(s):  
R T Surosky ◽  
R Strich ◽  
R E Esposito
Keyword(s):  
Vegetative Growth ◽  
Signal Transduction Pathway ◽  
Kinase Domain ◽  
Specific Protein ◽  
Transcript Stability ◽  
The Family ◽  
Two Factors ◽  
Genetic Epistasis ◽  
The Stability ◽  
Acetate Medium

We reported previously that early meiotic transcripts are highly unstable. These mRNAs exhibit half-lives of approximately 3 min when expressed during vegetative growth in glucose medium and are stabilized twofold during sporulation in acetate medium. Two genes, UME2 and UME5, that regulate the stability of meiosis-specific transcripts have been identified. The wild-type UME5 gene, which has been analyzed in detail, decreases the stability of all meiotic mRNAs tested approximately twofold when expressed during vegetative growth but has no effect on the half-lives of a number of vegetative mRNAs examined. The UME5 gene is dispensable for mitotic and meiotic development. Cells in which the entire UME5 gene has been deleted are viable, although the generation time is slightly longer and sporulation is less efficient. The UME5 transcript is constitutively expressed, and its stability is not autoregulated. The UME5 gene encodes a predicted 63-kDa protein with homology to the family of CDC28 serine/threonine-specific protein kinases. The kinase activity appears to be central to the function of the UME5 protein, since alteration of a highly conserved amino acid in the kinase domain results in a phenotype identical to that of a ume5 deletion. Genetic epistasis studies suggest that the UME2 and UME5 gene products act in the same pathway to regulate meiotic transcript stability. This pathway is independent of deadenylation and translation, two factors known to be important in regulating mRNA turnover. Significantly, the UME5-mediated destabilization of meiotic mRNAs occurs in glucose- but not in acetate-containing medium. Thus, the UME5 gene appears to participate in a glucose signal transduction pathway governing message stability.

The Authoritarian Spectrum Through the Prism of Individualism/Collectivism

2018 ◽  
Vol 11 (3) ◽  
pp. 47-68
Author(s):  
Ben Ahmed Hougua ◽  
El Amine Rachid ◽  
Siyouri Hind
Keyword(s):  
Professional Socialization ◽  
Main Idea ◽  
Categorical Variables ◽  
World Values Survey ◽  
Political Work ◽  
The Family ◽  
The Government ◽  
The Stability ◽  
The Relationship ◽  
General Configuration

The general orientation to authority in Moroccan society is associated with dynamics that characterize it at the basic social level. Respect for authority tends to take the form of a syndrome forged in the sphere of convergent styles of family, pedagogical, and professional socialization, before being expressed as political loyalty. This articulation is at the very heart of the theory of congruence developed by Harry Eickstein, and recently taken up by other political scientists. The main idea put forward by the proponents of this theory is that the stability of the government is brought to bear when the models of authority on which it rests enjoy social fascination. The purpose of this article is to study the relationship between respect for authority in the three spheres of social and political work based on World Values Survey (WVS) data on Moroccan society (N = 3651) including the family, workplace, and political world. The examination of the general configuration of the orientation to authority is parameterized according to the individualistic and collectivist profiles constructed in the WVS database, as categorical variables.

Stability Analysis for a Class of Linear Switched Positive Systems

2012 ◽  
Vol 562-564 ◽  
pp. 2084-2087
Author(s):  
Hui Ding ◽  
Xu Yang Lou
Keyword(s):  
Lyapunov Functions ◽  
Dwell Time ◽  
Positive Systems ◽  
Time Analysis ◽  
Arbitrary Switching ◽  
The Family ◽  
The Common ◽  
The Stability ◽  
Switched Positive Systems ◽  
Explicit Relation

This paper addresses stability properties of linear switched positive systems composed of continuous-time subsystems and discrete-time subsystems. Based on the common linear copositive Lyapunov functions, stability of the positive systems is discussed under arbitrary switching. Moreover, a sufficient condition on the minimum dwell time that guarantees the stability of linear switched positive systems. The dwell time analysis interprets the stability of linear switched positive systems through the distance between the eigenvector sets. Thus, an explicit relation in view of stability is obtained between the family of the involved subsystems and the set of admissible switching signals.

Structure-based engineering of glucose specificity in a family 10 xylanase from Streptomyces olivaceoviridis E-86

2012 ◽  
Vol 47 (3) ◽  
pp. 358-365 ◽  
Author(s):  
Hitomi Ichinose ◽  
Shaghik Diertavitian ◽  
Zui Fujimoto ◽  
Atsushi Kuno ◽  
Leila Lo Leggio ◽  
...  
Keyword(s):  
Streptomyces Olivaceoviridis ◽  
Family 10 Xylanase

scholarly journals Regulation of Major Cellulosomal Endoglucanases of Clostridium thermocellum Differs from That of a Prominent Cellulosomal Xylanase

2005 ◽  
Vol 187 (7) ◽  
pp. 2261-2266 ◽  
Author(s):  
Tali W. Dror ◽  
Adi Rolider ◽  
Edward A. Bayer ◽  
Raphael Lamed ◽  
Yuval Shoham
Keyword(s):  
Growth Rate ◽  
Clostridium Thermocellum ◽  
Transcript Level ◽  
Clear Correlation ◽  
Transcript Levels ◽  
Batch Cultures ◽  
Processive Endoglucanase ◽  
The Family ◽  
Genes Encoding ◽  
Family 10 Xylanase

ABSTRACT The expression of scaffoldin-anchoring genes and one of the major processive endoglucanases (CelS) from the cellulosome of Clostridium thermocellum has been shown to be dependent on the growth rate. For the present work, we studied the gene regulation of selected cellulosomal endoglucanases and a major xylanase in order to examine the previously observed substrate-linked alterations in cellulosome composition. For this purpose, the transcript levels of genes encoding endoglucanases CelB, CelG, and CelD and the family 10 xylanase XynC were determined in batch cultures, grown on either cellobiose or cellulose, and in carbon-limited continuous cultures at different dilution rates. Under all conditions tested, the transcript levels of celB and celG were at least 10-fold higher than that of celD. Like the major processive endoglucanase CelS, the transcript levels of these endoglucanase genes were also dependent on the growth rate. Thus, at a rate of 0.04 h−1, the levels of celB, celG, and celD were threefold higher than those obtained in cultures grown at maximal rates (0.35 h−1) on cellobiose. In contrast, no clear correlation was observed between the transcript level of xynC and the growth rate—the levels remained relatively high, fluctuating between 30 and 50 transcripts per cell. The results suggest that the regulation of C. thermocellum endoglucanases is similar to that of the processive endoglucanase celS but differs from that of a major cellulosomal xylanase in that expression of the latter enzyme is independent of the growth rate.

scholarly journals Farms as a resilience factors to land degradation in peri-urban areas

2013 ◽  
Vol 44 (2s) ◽  
Author(s):  
Paolo Zappavigna ◽  
Andrea Brugnoli
Keyword(s):  
Urban Areas ◽  
Structural Characteristics ◽  
Labour Force ◽  
Social Characteristics ◽  
The Family ◽  
External Conditions ◽  
Resilience Factors ◽  
The Stability ◽  
Economic Size

The purpose of this study was the analysis of the effects induced by urban pressures on the socio-economic and territorial characteristics of the rural peri-urban areas in order to identify planning and intervention strategies aimed at enhancing the quality of agriculture and landscape. A survey was conducted in the surroundings of Parma on farms located in the vicinity of urban areas. The structural, productive and social characteristics of the family-farm units were analyzed. The survey updated an identical survey, carried out in 1986, in which it was examined a sample of 208 farms. The units surveyed were evaluated in two aspects: the “vitality”, which takes into account the structural characteristics (size, production, labour force, etc.), and the “stability”, in which a crucial role is played by the age of the conductor and the presence of a successor. It was found that only 28% of the original farm sample is still alive, one third has disappeared, 30% was absorbed by existing farms, 8% has been abandoned. The factors most favourable to the survival resulted those referred to the vitality, especially the physical and economic size of the farm, the presence of cattle, the percentage of land in property, the presence of young labour. Among the factors that predispose to the abandonment, the urbanization processes were found to be determinants, in terms of expansion of both the built-up area and of that planned as urbanisable. The research has highlighted the importance of the vitality of the farms together with a context that has maintained its original rural features. These combined aspects can better define what we call the resiliency of the landfarms system i.e. the capability of positively reacting to the variable modifications of the internal and external conditions.

scholarly journals The Stability Paradox: The Two-Parent Paradigm and the Perpetuation of Violence Against Women in Termination of Parental Rights and Custody Cases

2021 ◽  
pp. 311
Author(s):  
Judith Lewis
Keyword(s):  
Violence Against Women ◽  
Partner Violence ◽  
Nuclear Family ◽  
Parental Rights ◽  
Sexual Offense ◽  
Best Interest ◽  
Termination Of Parental Rights ◽  
The Family ◽  
Women And Children ◽  
The Stability

Despite changing family compositions, entrenched in family law is the antiquated idea that a two-parent household, or its approximation vis-à-vis a shared custody arrangement, promotes stability and integrity and, thus, is in the best interest of the child. Yet, the concept that the two-parent household (or shared involvement of both parents in the child’s life if the parents separate) promotes stability for the family and is best for the child is a dangerous fallacy. When rape or intimate partner violence (IPV) is present, or the re-occurrence of violence remains a threat, the family unit is far from stable. This Article explores the legal system’s glorification of the nuclear family, its resistance to shifting away from the two-parent paradigm, and how this resistance creates a stability paradox and perpetuates violence against women and children. The harmful impact that the nuclear family paradigm has on families is further explored by an examination of the statutory constructs and judicial interpretations of termination of parental rights (TPR) and custody statutes in cases where a child is conceived as a result of rape or exposed to ongoing IPV. Cases are utilized to examine how courts have interpreted parental rights statutes where a child is conceived as a result of rape. Additionally, a hypothetical case is discussed to explore arguments that may be advanced in TPR cases where children are exposed to ongoing IPV. The Article finds that although there are inherent problems in enacting statutes to terminate parental rights in cases involving rape or IPV, legislation is also a necessary tool for survivors. Model legislation is proposed for termination of parental rights in cases where a child is conceived as a result of a sexual offense or when a child is exposed to ongoing IPV.

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