Molecular detection and antibiogram of Escherichia coli O157 isolated from subclinical mastitis affected cows at Baghabari, Sirajganj

2021 ◽  
Vol 1 (2) ◽  
pp. 6
Author(s):  
S Farhad ◽  
Md Shahid ◽  
Md Mahmud ◽  
Ajran Kabir ◽  
Shanta Das ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Detection ◽  
Subclinical Mastitis ◽  
Escherichia Coli O157

scholarly journals BIOCHEMICAL STUDIES IN FOOD SPOILAGE VIA MOLECULAR DETECTION OF SOME ENTEROBACTERIACEAE FOOD BORNE BACTERIA USING MALDI-TOF

2020 ◽  
Vol 21 (supplement 1) ◽  
Author(s):  
Heba M.Karmy ◽  
Mahfouz A.Abdelaziz ◽  
Doaa M. Ghaith ◽  
Nashwa M.Helmy ◽  
Kamla M. El–Saied
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
Molecular Detection ◽  
Disease Outbreaks ◽  
Standard Technique ◽  
Foodborne Diseases ◽  
Escherichia Coli O157 ◽  
Tissue Samples ◽  
Maldi Tof ◽  
Food Borne

Foodborne diseases are caused by consumption of food spoiled by pathogens or their toxins. Sampling beef meat and chicken samples from local markets which is additionally affecting foodborne disease outbreaks, making food safety a universal issue. Biochemical classical microbiological and targeted molecular profiling of Enterobactericea using real time PCR, MALDI TOF as well as sequencing confirmation techniques were performed and compromised for speciation of possible foodborne pathogens in meat and tissue samples in randomly collected 300 samples is traced with their health riskimpact on human. Sum of 68 samples out of 300 were speciated; 28 meat (18 Escherichia coli O157:H7, 10 Salmonella), 18 (10 Escherichia coli O157:H7, 8 Salmonella) and 22 (10 E-coil, 12 Salmonella). Six representative positive samples of both Salmonella and Escherichia coli O157:H7 were selected for further confirmation by sequencing as gold standard technique.

Bedeutung von Escherichia coli O157 beim Schlachtschaf in der Schweiz

2006 ◽  
Vol 148 (6) ◽  
pp. 289-295 ◽  
Author(s):  
C. Zweifel ◽  
M. Kaufmann ◽  
J. Blanco ◽  
R. Stephan
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157

Molecular profiling and antimicrobial susceptibility of Escherichia coli O157:H7 isolated in Bulgaria

2020 ◽  
Vol 23 (3) ◽  
pp. 310-318
Author(s):  
K. Koev ◽  
T. Stoyanchev ◽  
G. Zhelev ◽  
P. Marutsov ◽  
K. Gospodinova ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157 ◽  
Acid Sensitivity ◽  
Lower Extent ◽  
Phenotype Analysis ◽  
Anal Swab ◽  
Amoxicillin Clavulanic Acid ◽  
Primary Identification ◽  
Stx1 Gene ◽  
Cattle Farms

The purpose of this study was to detect the presence of shiga-toxin producing Escherichia coli (STEC) in faeces of healthy dairy cattle and to determine the sensitivity of isolates to several anti­microbial drugs. A total of 1,104 anal swab samples originating from 28 cattle farms were examined. After the primary identification, 30 strains were found to belong to serogroup О157. By means of conventional multiplex PCR, isolates were screened for presence of resistance genes stx1, stx2 and eaeА. Twenty-nine strains possesses amplicons with a size corresponding to genes stx2 and eaeA, one had amplicons also for the stx1 gene and one lacked amplicons of all three genes. Twenty-eight strains demonstrated amplicons equivalent to gene H7. The results from phenotype analysis of resistance showed preserved sensitivity to ceftriaxone, ceftazidime, cefotaxime, cephalothin, streptomycin, gentamicin, tetracycline, enrofloxacin and combinations sulfamethoxazole/trimethoprim and amoxicillin/clavulanic acid. Sensitivity to ampicillin was relatively preserved, although at a lower extent.

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