scholarly journals Some hematological values and alpha-naphthyl acetate esterase (ANAE)-positive lymphocyte ratios in Jaydara sheep

2016 ◽  
Vol 3 (1) ◽  
pp. 8
Author(s):  
Nurcan Donmez ◽  
Hasan Donmez ◽  
Ihsan Kadiralieva
Keyword(s):  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate ◽  
Hematological Values

scholarly journals Chromosome pattern in childhood acute nonlymphocytic leukemia (ANLL)

Blood ◽  
1982 ◽  
Vol 60 (2) ◽  
pp. 389-399 ◽  
Author(s):  
Y Kaneko ◽  
JD Rowley ◽  
HS Maurer ◽  
D Variakojis ◽  
JW Moohr
Keyword(s):  
Age Groups ◽  
Normal Karyotype ◽  
Chromosome Abnormalities ◽  
Host Susceptibility ◽  
Leukemic Cells ◽  
Karyotypic Analysis ◽  
Chromosome Pattern ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate

Abstract We studied the karyotype in 26 children with ANLL, which was diagnosed on the basis of the FAB classification. Clonal chromosome abnormalities were found in 21 of 26 patients. Four patients, including 3 with Down's syndrome, had AML(M1). Nine patients, including 3 with t(8;21), had AML(M2). All 3 patients with APL(M3) had t(15;17). Four patients had AMMOL(M4); 3 of these had a normal karyotype. Six patients had AMOL(M5); 5 and 11q rearrangements, and 3 of these had a break in 11q23. Only one patient had EL(M6), and he had a normal karyotype. One patient with t(11;19), classified as AML(M2) on Wright-Giemsa-stained cells, had a strong alpha-naphthyl acetate esterase reaction, indicating that the leukemic cells had a cytochemical feature characteristic of monocytes. Whereas t(8;21) and t(15;17) are uniquely associated with AML(M2) and APL(M3), respectively, the 11q rearrangements are also seen in AML(M1/M2), although they are more common in AMOL(M5) and AMMOL(M4). The case with t(11;19) suggests that cells with 11q rearrangements and with AML(M1/M2) may have both monocytic and granulocytic features. When we used our data and previous reports on 243 aneuploid patients (169 adults and 74 children) to correlate the chromosome abnormalities with patient age, we found differences in the chromosome pattern seen among various age groups. This suggests that different etiologic factors as well as changes in host susceptibility may influence the development of and the karyotypic pattern in the various types of leukemia. Moreover, the frequency of various chromosome abnormalities in childhood ANLL can provide a baseline for comparison of the frequency of the same abnormality in adults. The karyotypic analysis of childhood ANLL is important not only because of the information that can be obtained about childhood ANLL, but also because the data can provide substantial insight into the etiology of ANLL in adults.

scholarly journals Rapid method for identification of macrophages in suspension by acid alpha-naphthyl acetate esterase activity.

1983 ◽  
Vol 31 (7) ◽  
pp. 960-963 ◽  
Author(s):  
D L Ennist ◽  
K H Jones
Keyword(s):  
Azo Dye ◽  
Close Agreement ◽  
Staining Procedure ◽  
Inexpensive Method ◽  
Morphological Criteria ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Rats And Mice ◽  
Naphthyl Acetate ◽  
Supravital Staining

A supravital staining procedure for the identification of macrophages in cell suspension using a modification of a standard cytochemical assay for alpha-naphthyl acetate esterase (ANAE) activity is described. Macrophages are stained an intense red-brown after 5 min incubation in a buffer using ANAE as the substrate and hexazonium pararosaniline as the coupler for the azo dye. There is close agreement in the number of ANAE-positive cells found and the number of macrophages identified in smears by morphological criteria, by phagocytosis, and by the presence of Fc receptors. Therefore, this stain provides a quick, inexpensive method to estimate the number of macrophages present in suspensions of lymphocytic tissues from rats and mice.

scholarly journals Acute megakaryoblastic leukemia in early childhood

Blood ◽  
1983 ◽  
Vol 62 (1) ◽  
pp. 92-98
Author(s):  
WC Chan ◽  
RK Brynes ◽  
TH Kim ◽  
A Verras ◽  
C Schick ◽  
...  
Keyword(s):  
Early Childhood ◽  
Cytosine Arabinoside ◽  
Chromosomal Abnormalities ◽  
Severe Thrombocytopenia ◽  
Acute Megakaryoblastic Leukemia ◽  
Megakaryoblastic Leukemia ◽  
Sudan Black B ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate

Two girls, each less than 2 yr of age, developed acute megakaryoblastic leukemia (malignant myelosclerosis). Both presented with anemia, severe thrombocytopenia, and a low percentage of blasts in their peripheral blood. Their marrow showed marked reticulin fibrosis with an increase in blasts and immature megakaryocytes. The blasts stained negatively for myeloperoxidase and Sudan Black B, but showed acid phosphatase (ACP) and alpha-naphthyl acetate esterase (ANAE) activity inhibitable by sodium fluoride. They were identified as megakaryoblasts by the platelet peroxidase reaction. Cytogenetic studies showed multiple chromosomal abnormalities in both cases. Chemotherapy with vincristine, prednisone, and L-asparaginase was without effect, while daunorubicin and cytosine arabinoside induced a complete remission in one case. The second case responded to a combination of cytosine arabinoside, daunorubicin, and 6-thioguanine. This article documents that acute megakaryoblastic leukemia occurs in early childhood and describes its clinical, pathologic, and cytogenetic features. Previous reports of childhood “myelofibrosis” are reviewed, and their possible relationship with acute megakaryoblastic leukemia is discussed.

scholarly journals Cell marker analysis in acute monocytic leukemias

Blood ◽  
1977 ◽  
Vol 49 (6) ◽  
pp. 895-901
Author(s):  
B Koziner ◽  
S McKenzie ◽  
D Straus ◽  
B Clarkson ◽  
RA Good ◽  
...  
Keyword(s):  
Leukemic Cells ◽  
Acute Monocytic Leukemia ◽  
Cell Marker ◽  
Monocytic Leukemia ◽  
Differentiation Markers ◽  
Marker Analysis ◽  
Multiple Differentiation ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate

Leukemic cells from nine cases of acute monocytic leukemia (AMoL) were characterized by multiple differentiation markers. Cells in most cases were phagocytic, carried an Fc receptor, and stained positively for alpha-naphthyl acetate esterase but negatively for naphthol AS-D chloroacetate esterase. However, subtle differences in marker expression were observed which suggested different degrees of leukemic cellular maturation or activation. Cell marker analysis proved to be a useful adjunct to conventional morphology in confirming the diagnosis and the recognition of the neoplastic cells in AMoL, and may ultimately provide insight into the functional state of these cells.

Quinidine-induced inhibition of leukocyte esterases.

1988 ◽  
Vol 34 (3) ◽  
pp. 518-524 ◽  
Author(s):  
O T Markovic ◽  
D S Young ◽  
N S Markovic
Keyword(s):  
Image Processing ◽  
Enzyme Activity ◽  
Polymorphonuclear Neutrophils ◽  
Dose Effect ◽  
Quinoline Ring ◽  
High Concentration ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate ◽  
Very High

Abstract We have demonstrated the feasibility of a system based on image processing to measure enzyme activity inside morphologically classified individual cells. When used in this system, quinidine inhibited naphthol AS-D chloroacetate esterase in polymorphonuclear neutrophils and alpha-naphthyl acetate esterase and alpha-naphthyl butyrate esterase in monocytes. These effects were dependent on the duration of exposure and on drug concentration. Dose-effect relationships were established and the concentration at which 50% inhibition (ID-50) occurred was used as a reference point to compare the toxicity of different compounds. Chloroquine, primaquine, and quinine (which, like quinidine, possess a quinoline ring) also affected the esterases, but had no effect on several other enzymes. Neostigmine inhibited the esterases we studied but only at a very high concentration. Various chemicals inhibited the enzymes within the cells, as has been demonstrated for the enzymes in plasma.

scholarly journals Cell marker analysis in acute monocytic leukemias

Blood ◽  
1977 ◽  
Vol 49 (6) ◽  
pp. 895-901 ◽  
Author(s):  
B Koziner ◽  
S McKenzie ◽  
D Straus ◽  
B Clarkson ◽  
RA Good ◽  
...  
Keyword(s):  
Leukemic Cells ◽  
Acute Monocytic Leukemia ◽  
Cell Marker ◽  
Monocytic Leukemia ◽  
Differentiation Markers ◽  
Marker Analysis ◽  
Multiple Differentiation ◽  
Acetate Esterase ◽  
Alpha Naphthyl Acetate Esterase ◽  
Naphthyl Acetate

Abstract Leukemic cells from nine cases of acute monocytic leukemia (AMoL) were characterized by multiple differentiation markers. Cells in most cases were phagocytic, carried an Fc receptor, and stained positively for alpha-naphthyl acetate esterase but negatively for naphthol AS-D chloroacetate esterase. However, subtle differences in marker expression were observed which suggested different degrees of leukemic cellular maturation or activation. Cell marker analysis proved to be a useful adjunct to conventional morphology in confirming the diagnosis and the recognition of the neoplastic cells in AMoL, and may ultimately provide insight into the functional state of these cells.

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