Microscopical Study of White and Red Pulp of Spleen of Young Porcine (Sus Scrofa) Spleen

In spite of various attempts, conclusive evidence to explain blood passage in the splenic red pulp does not seem to have been presented. Scanning electron microscope (SEM) observations on the rabbit spleen, originally performed by us, revealed that the sinus was lined by a perforated lattice composed of longitudinally extended rod cells and transverse cytoplasmic processes, and that perforations in the lattice were continuous to the spaces among the stellate reticulum cells of the cord. In the present study the observation was extended to the dog and rat spleens, in which the cord is more developed than in the rabbit in order to clarify the possible differences in the fine structure of the sinus wall. An attempt was also made to examine the development and distribution of macrophage in the blood passage of the red pulp.Spleens were washed and fixed by perfusion with Ringer solution and then with buffered glutaraldehyde. Small tissue cubes were dehydrated with acetone, dried in air and heated with gold. Observations were made by a JEOL SEM Type-3. One air dried tissue cube was cut into small pieces and post fixed with buffered OsO4 for examination under the transmission electron microscope (TEM).

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Etching of Polymers by Oxygen Plasma

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100101797 ◽

1968 ◽

Vol 26 ◽

pp. 408-409

Author(s):

Virgil Peck ◽

W. L. Carter

Keyword(s):

Molecular Weight ◽

Oxygen Plasma ◽

Flow Time ◽

Microscopical Study ◽

Surface Layers ◽

Organic Vapors ◽

Sample Position ◽

Accuracy Of Measurement ◽

Bulk Polymers ◽

Electron Microscopical

Any electron microscopical study of the morphology of bulk polymers has throughout the years been hampered by the lack of any real ability to produce meaningful surface variations for replication. True etching of polymers should show crystalline and amorphous regions in some form of relief. The use of solvents, acids, organic vapors, and inert ion bombardment to etch samples has proved to be useful only in limited applications. Certainly many interpretations of these results are subject to question.The recent use of a radiofrequency (R. F.) plasma of oxygen to degrade and remove organic material with only minor heating has opened a new possibility for etching polymers. However, rigid control of oxygen flow, time, current, and sample position are necessary in order to obtain reproducible results. The action is confined to surface layers; the molecular weight of the polymer residue after heavy etching is the same as the molecular weight of the polymer before attack, within the accuracy of measurement.

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Silicon layers grown over SiO2 by liquid phase epitaxy: Electron Microscopical study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010017596x ◽

1990 ◽

Vol 48 (4) ◽

pp. 566-567

Author(s):

F. Banhart ◽

F.O. Phillipp ◽

R. Bergmann ◽

E. Czech ◽

M. Konuma ◽

...

Keyword(s):

Electron Microscopy ◽

Liquid Phase ◽

Plasma Etching ◽

Liquid Phase Epitaxy ◽

Three Dimensional ◽

Microscopical Study ◽

Sample Temperature ◽

Structural Defects ◽

Si Substrates ◽

Etched Surface

Defect-free silicon layers grown on insulators (SOI) are an essential component for future three-dimensional integration of semiconductor devices. Liquid phase epitaxy (LPE) has proved to be a powerful technique to grow high quality SOI structures for devices and for basic physical research. Electron microscopy is indispensable for the development of the growth technique and reveals many interesting structural properties of these materials. Transmission and scanning electron microscopy can be applied to study growth mechanisms, structural defects, and the morphology of Si and SOI layers grown from metallic solutions of various compositions.The treatment of the Si substrates prior to the epitaxial growth described here is wet chemical etching and plasma etching with NF3 ions. At a sample temperature of 20°C the ion etched surface appeared rough (Fig. 1). Plasma etching at a sample temperature of −125°C, however, yields smooth and clean Si surfaces, and, in addition, high anisotropy (small side etching) and selectivity (low etch rate of SiO2) as shown in Fig. 2.

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LIGHT AND ELECTRON MICROSCOPICAL STUDY OF THE EFFECT OF OESTROGEN ON THE CHICKEN OVIDUCT

Acta Endocrinologica ◽

10.1530/acta.0.0560391 ◽

1967 ◽

Vol 56 (3) ◽

pp. 391-402 ◽

Cited By ~ 12

Author(s):

H. I. Ljungkvist

Keyword(s):

Secretory Granules ◽

Apical Cell ◽

Cell Types ◽

Microscopical Study ◽

List Type ◽

New Production ◽

General Increase ◽

Chicken Oviduct ◽

Electron Microscopical ◽

Aortic Perfusion

ABSTRACT Oviducts from 20 one-day old chickens were used. Ten chickens were injected subcutaneously with 0.2 mg oestradiol for 5 days, the remaining ones serving as controls. The chickens were fixed by an aortic perfusion with 2.5% glutaraldehyde in phosphate buffer, pH 7.2. The treatment with oestrogen resulted in the following changes: general increase in oviduct length and thickness, differentiation of the epithelial membrane into three cell types: basal, apical and gland cells, increase in the number of cilia in the apical cell, probably due to a new production of cilia, formation of secretory granules in the vaginal epithelium as seen by light microscopy, formation of proteinlike secretory granules in the apical cell as seen by electron microscopy, increase in protein synthesis, observed as an augmentation of the endoplasmic reticulum.

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ЛИПИДНЫЙ СОСТАВ ОБРАЗЦОВ МЫШЕЧНОЙ И ЖИРОВОЙ ТКАНИ У СВИНЕЙ (Sus scrofa domesticus Erxleben, 1777) ПОРОДЫ ДЮРОК: ОСОБЕННОСТИ И КОРРЕЛЯЦИИ

Sel skokhozyaistvennaya Biologiya ◽

10.15389/agrobiology.2018.6.1262rus ◽

2018 ◽

Vol 53 (6) ◽

pp. 1262-1273

Author(s):

А.C. ПАВЛОВА ◽

◽

А.А. ВАНЮШКИНА ◽

Е.А. ЮШИНА ◽

А.Н. ЕГОРОВА ◽

...

Keyword(s):

Sus Scrofa ◽

Sus Scrofa Domesticus

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Activation of regeneration of red and white pulp of the spleen after the combined transplantation of HSC and MSCS in terms of exposure to ionizing radiation

ZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia» ◽

10.25557/0031-2991.2017.02.22-27 ◽

2017 ◽

pp. 22-27

Author(s):

И.Ю. Маклакова ◽

Д.Ю. Гребнев ◽

А.П. Ястребов

Keyword(s):

Stem Cells ◽

Ionizing Radiation ◽

Hematopoietic Stem Cells ◽

Morphometric Parameters ◽

Hematopoietic Stem ◽

Lymphoid Follicles ◽

Average Area ◽

Direct Technique ◽

Average Size ◽

Red Pulp

Цель - изучение влияния сочетанной трансплантации мультипотентных мезенхимальных стромальных (ММСК) и гемопоэтических стволовых клеток (ГСК), выделенных из плаценты, на регенерацию белой и красной пульпы селезенки в физиологических условиях и в условиях воздействия ионизующего излучения. Методика. Эксперименты выполнены белых лабораторных беспородных мышах-самцах. Облучение животных проводилось на гамма-терапевтической установке типа АГАТ-С с радионуклидным источником Co-60 типа ГИК-8-4, поглощенная доза составила 4,0 Гр, мощность поглощенной дозы 20 сГр/мин. Животным опытной группы внутривенно вводились аллогенные ММСК и ГСК соответственно в дозе 6 млн клеток/кг и 330 тыс. клеток/кг, суспендированные в 0,2 мл 0,9% раствора NaCl. Выделение гемопоэтических стволовых клеток осуществлялось методом прямой иммуномагнитной сепарации. Проводили морфометрию лимфоидных фолликулов селезенки (средняя площадь, средняя площадь В-зоны, средняя площадь герминативного центра, средняя площадь T-зоны), а также определялось среднее расстояние между центрами фолликулов и средняя клеточность красной пульпы. Результаты. Показано, что после воздействия ионизирующего излучения на фоне сочетанной трансплантации ММСК и ГСК происходит увеличение размеров лимфоидного фолликула за счет площади B-зоны фолликула, площади герминативного центра фолликула, восстановление содержания лимфобластов, пролимфоцитов и лимфоцитов до значений нормы. На фоне трансплантации ММСК и ГСК в условиях лучевой нагрузки установлено увеличение плотности клеток в красной пульпе селезенки и, как следствие, увеличение расстояния между центрами лимфоидных фолликулов. Увеличение плотности клеток в красной пульпе происходит как за счет увеличения содержания эритроидных клеток, так и за счет увеличения гранулоцитов. Заключение. Проведенные исследования свидетельствуют об эффективности сочетанной трансплантации ММСК и ГСК в отношении основных морфометрических показателей селезенки после воздействия ионизирующего излучения. The purpose of this work was to study the effect of combined transplantation of multipotent mesenchymal stromal (MSCS) and hematopoietic stem cells (HSCs) isolated from the placenta, on the regeneration of white and red pulp of the spleen under physiological conditions and in conditions of exposure to ionizing radiation. Methods. The experiments were performed with laboratory mice-males. We studied the influence of ionizing radiation dose of 4.0 Gy. Animals of the experimental group were intravenously infused into MMSC and GSK respectively at a dose of 6 million cells/kg and 330 thousand cells/kg, suspended in 0.2 ml of 0.9% NaCl solution. The selection of hematopoietic stem cells was carried out using the direct technique of immune magnetic separation. Were studied the following morphometric parameters of the spleen: the average area of lymphoid follicles, the average area of zone of lymphoid follicles, average size of germinal center of lymphoid follicles, average size T-zones of lymphoid follicles, the average distance between the centers of the follicles, the average cellularity of the red pulp. Results. As a result, of research obtained that after exposure to ionizing radiation on the background of combined transplantation of HSC and MSCS there is an increase in size of lymphoid follicle at the expense of area B-zone of the follicle, the area germinative center of the follicle, restoring the content of lymphoblasts and lymphoblasts and lymphocytes to normal values. On the background of transplantation MMSC and GSK in terms of radiation exposure changes and the red pulp of the spleen. The increase in the density of cells in the red pulp of the spleen and, as a consequence, of the increase of the distance between the centers of lymphoid follicles. The increase in the density of cells in the red pulp occurs due to the increase in the content of erythroid cells and by increasing granulocytes. Key words: ionizing radiation, multipotent mesenchymal stromal cells, hematopoietic stem cells, spleen, regeneration. Conclusion. Studies have shown the effectiveness of combined transplantation MSC and GSK in respect of the main morphometric parameters of the spleen after exposure to ionizing radiation.

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