scholarly journals Genome-Shuffling-Improved Acid Tolerance and Lactic Acid Production in Lactobacillus plantarum for Commercialization

2011 ◽  
Vol 5 (1) ◽  
pp. 21-26 ◽  
Author(s):  
LITA TRIRATNA ◽  
BUDI SAKSONO ◽  
LINDA SUKMARINI ◽  
ASEP SUPARMAN
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Genome Shuffling ◽  
Acid Tolerance

d-lactic acid production from cellooligosaccharides and β-glucan using l-LDH gene-deficient and endoglucanase-secreting Lactobacillus plantarum

2009 ◽  
Vol 85 (3) ◽  
pp. 643-650 ◽  
Author(s):  
Kenji Okano ◽  
Qiao Zhang ◽  
Shogo Yoshida ◽  
Tsutomu Tanaka ◽  
Chiaki Ogino ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactobacillus Plantarum ◽  
Acid Production ◽  
Lactic Acid Production

scholarly journals Plasticity of the Pyruvate Node Modulates Hydrogen Peroxide Production and Acid Tolerance in Multiple Oral Streptococci

2017 ◽  
Vol 84 (2) ◽  
Author(s):  
Xingqun Cheng ◽  
Sylvio Redanz ◽  
Nyssa Cullin ◽  
Xuedong Zhou ◽  
Xin Xu ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Environmental Conditions ◽  
Dental Plaque ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Acid Tolerance ◽  
Oral Bacteria ◽  
Low Ph ◽  
Content Type ◽  
Pyruvate Node

ABSTRACTCommensalStreptococcus sanguinisandStreptococcus gordoniiare pioneer oral biofilm colonizers. Characteristic for both is the SpxB-dependent production of H2O2, which is crucial for inhibiting competing biofilm members, especially the cariogenic speciesStreptococcus mutans. H2O2production is strongly affected by environmental conditions, but few mechanisms are known. Dental plaque pH is one of the key parameters dictating dental plaque ecology and ultimately oral health status. Therefore, the objective of the current study was to characterize the effects of environmental pH on H2O2production byS. sanguinisandS. gordonii.S. sanguinisH2O2production was not found to be affected by moderate changes in environmental pH, whereasS. gordoniiH2O2production declined markedly in response to lower pH. Further investigation into the pyruvate node, the central metabolic switch modulating H2O2or lactic acid production, revealed increased lactic acid levels forS. gordoniiat pH 6. The bias for lactic acid production at pH 6 resulted in concomitant improvement in the survival ofS. gordoniiat low pH and seems to constitute part of the acid tolerance response ofS. gordonii. Differential responses to pH similarly affect other oral streptococcal species, suggesting that the observed results are part of a larger phenomenon linking environmental pH, central metabolism, and the capacity to produce antagonistic amounts of H2O2.IMPORTANCEOral biofilms are subject to frequent and dramatic changes in pH.S. sanguinisandS. gordoniican compete with caries- and periodontitis-associated pathogens by generating H2O2. Therefore, it is crucial to understand howS. sanguinisandS. gordoniiadapt to low pH and maintain their competitiveness under acid stress. The present study provides evidence that certain oral bacteria respond to environmental pH changes by tuning their metabolic output in favor of lactic acid production, to increase their acid survival, while others maintain their H2O2production at a constant level. The differential control of H2O2production provides important insights into the role of environmental conditions for growth competition of the oral flora.

scholarly journals Production of L-lactic acid from Cassava peel wastes using single and mixed cultures of Rhizopus oligosporus and Lactobacillus plantarum

2014 ◽  
Vol 20 (4) ◽  
pp. 457-461 ◽  
Author(s):  
Ogbonnaya Nwokoro
Keyword(s):  
Lactic Acid ◽  
Mixed Culture ◽  
Lactobacillus Plantarum ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Reducing Sugar ◽  
Rhizopus Oligosporus ◽  
Mixed Culture Fermentation ◽  
Cassava Peels ◽  
Reducing Sugar Concentration

Production of L-lactic acid using cultures of Rhizopus oligosporus and Lactobacillus plantarum was investigated. Cassava peels were hydrolyzed by boiling for 1 h in either NaOH or HCl solutions followed by neutralization to a pH of 6.2. Reducing sugar produced from the hydrolysates increased with increasing concentrations of alkali or acid. Samples hydrolyzed with HCl produced a maximum reducing sugar concentration of 402 mg/g substrate while alkali hydrolyzed samples produced a maximum reducing sugar concentration of 213 mg/g substrate. Hydrolysates were amended with 0.5% ammonium sulphate solution and inoculated with either single or mixed cultures of Rhizopus oligosporus and Lactobacillus plantarum and incubated for 48 h for lactic acid production. The best lactic acid production of 50.2 g/100g substrate was observed in a mixed culture fermentation of acid hydrolyzed peels. Mixed culture fermentation of alkali hydrolyzed peels produced a maximum lactic acid concentration of 36.4 g/100g substrate. Un hydrolyzed cassava peels inoculated with a mixed culture of the microorganisms produced only 4.6 g/100g substrate. This work reports an efficient use of cassava peels for bio-product formation through microbial fermentation.

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