The Influence of Oil Concentration, Nitrogen and Phosphorus Composition on Crude Oil Biodegradation by Epyzim and Mixed

2008 ◽  
Vol 2 (3) ◽  
pp. 146-149
Author(s):  
ERLIZA NOOR ◽  
LINAWATI HARDJITO
Keyword(s):  
Crude Oil ◽  
Nitrogen And Phosphorus ◽  
Oil Biodegradation ◽  
Oil Concentration

Response surface analysis and modeling of n-alkanes removal through bioremediation of weathered crude oil

2011 ◽  
Vol 63 (4) ◽  
pp. 618-626 ◽  
Author(s):  
Leila Mohajeri ◽  
Hamidi Abdul Aziz ◽  
Mohammad Ali Zahed ◽  
Soraya Mohajeri ◽  
Shamsul Rahman Mohamed Kutty ◽  
...  
Keyword(s):  
Crude Oil ◽  
Response Surface ◽  
Natural Attenuation ◽  
Coastal Sediments ◽  
Nitrogen And Phosphorus ◽  
Bacterial Inoculum ◽  
Oil Concentration ◽  
Excessive Nutrients ◽  
Optimized Conditions ◽  
Central Composite

Central composite design (CCD) and response surface methodology (RSM) were employed to optimize four important variables, i.e. amounts of oil, bacterial inoculum, nitrogen and phosphorus, for the removal of selected n-alkanes during bioremediation of weathered crude oil in coastal sediments using laboratory bioreactors over a 60 day experimentation period. The reactors contained 1 kg soil with different oil, microorganisms and nutrients concentrations. The F Value of 26.89 and the probability value (P < 0.0001) demonstrated significance of the regression model. For crude oil concentration of 2, 16 and 30 g per kg sediments and under optimized conditions, n-alkanes removal was 97.38, 93.14 and 90.21% respectively. Natural attenuation removed 30.07, 25.92 and 23.09% n-alkanes from 2, 16 and 30 g oil/kg sediments respectively. Excessive nutrients addition was found to inhibit bioremediation.

scholarly journals Evaluation of crude oil biodegradation using mixed fungal cultures

PLoS ONE ◽  
2021 ◽  
Vol 16 (8) ◽  
pp. e0256376
Author(s):  
Abeer R. M. Abd El-Aziz ◽  
Monira R. Al-Othman ◽  
Sameh M. Hisham ◽  
Shereen M. Shehata
Keyword(s):  
Crude Oil ◽  
Aspergillus Terreus ◽  
Date Palm ◽  
Incubation Temperature ◽  
Normal Alkanes ◽  
Oil Biodegradation ◽  
Initial Ph ◽  
Polycyclic Aromatic ◽  
Oil Concentration ◽  
Fungal Consortium

The use of potent fungal mixed cultures is a promising technique for the biodegradation of crude oil. Four isolates of fungi, namely, Alternaria alternata (AA-1), Aspergillus flavus (AF-3), Aspergillus terreus (AT-7), and Trichoderma harzianum (TH-5), were isolated from date palm soil in Saudi Arabia. The mixed fungal of the four isolates have a powerful tool for biodegradation up to 73.6% of crude oil (1%, w/v) in 14 days. The fungal consortium no. 15 containing the four isolates (1:1:1:1) performed significantly better as a biodegradation agent than other consortium in a variety of environmental factors containing crude oil concentration, incubation temperature, initial pH, biodegradation time and the salinity of the medium. The fungal consortium showed better performance in the biodegradation of normal alkanes (n-alkanes) than that of the polycyclic aromatic hydrocarbons (PAHs); the biodegradation efficiency of normal alkanes of the fungal consortium (67.1%) was clearly high than that of the PAHs (56.8%).

Effect of Initial Oil Concentration and Dispersant on Crude Oil Biodegradation in Contaminated Seawater

2010 ◽  
Vol 84 (4) ◽  
pp. 438-442 ◽  
Author(s):  
Mohammad Ali Zahed ◽  
Hamidi Abdul Aziz ◽  
Mohamed Hasnain Isa ◽  
Leila Mohajeri
Keyword(s):  
Crude Oil ◽  
Oil Biodegradation ◽  
Oil Concentration

scholarly journals Effects of Dispersants and Biosurfactants on Crude-Oil Biodegradation and Bacterial Community Succession

2021 ◽  
Vol 9 (6) ◽  
pp. 1200
Author(s):  
Gareth E. Thomas ◽  
Jan L. Brant ◽  
Pablo Campo ◽  
Dave R. Clark ◽  
Frederic Coulon ◽  
...  
Keyword(s):  
Crude Oil ◽  
Early Stage ◽  
Niche Partitioning ◽  
Hydrocarbon Biodegradation ◽  
Hydrocarbonoclastic Bacteria ◽  
Bacterial Response ◽  
Oil Biodegradation ◽  
Interfacial Surface ◽  
Degrading Bacteria ◽  
Oil Spill Response

This study evaluated the effects of three commercial dispersants (Finasol OSR 52, Slickgone NS, Superdispersant 25) and three biosurfactants (rhamnolipid, trehalolipid, sophorolipid) in crude-oil seawater microcosms. We analysed the crucial early bacterial response (1 and 3 days). In contrast, most analyses miss this key period and instead focus on later time points after oil and dispersant addition. By focusing on the early stage, we show that dispersants and biosurfactants, which reduce the interfacial surface tension of oil and water, significantly increase the abundance of hydrocarbon-degrading bacteria, and the rate of hydrocarbon biodegradation, within 24 h. A succession of obligate hydrocarbonoclastic bacteria (OHCB), driven by metabolite niche partitioning, is demonstrated. Importantly, this succession has revealed how the OHCB Oleispira, hitherto considered to be a psychrophile, can dominate in the early stages of oil-spill response (1 and 3 days), outcompeting all other OHCB, at the relatively high temperature of 16 °C. Additionally, we demonstrate how some dispersants or biosurfactants can select for specific bacterial genera, especially the biosurfactant rhamnolipid, which appears to provide an advantageous compatibility with Pseudomonas, a genus in which some species synthesize rhamnolipid in the presence of hydrocarbons.

Crude oil biodegradation potential of biosurfactant-producing Pseudomonas aeruginosa and Meyerozyma sp.

2021 ◽  
pp. 126276
Author(s):  
Ramla Rehman ◽  
Muhammad Ishtiaq Ali ◽  
Naeem Ali ◽  
Malik Badshah ◽  
Mazhar Iqbal ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Crude Oil ◽  
Oil Biodegradation

scholarly journals Biotreatability of Crude Oil Polluted Aquatic Environment Using Indigenous Hydrocarbon Utilizing Bacteria

2020 ◽  
pp. 52-62
Author(s):  
Tudararo-Aherobo Laurelta ◽  
Okotie Sylvester ◽  
Ataikiru Tega ◽  
Stephen Avwerosuoghene
Keyword(s):  
Crude Oil ◽  
Contaminated Soils ◽  
Aquatic Environment ◽  
Bacterial Counts ◽  
A Value ◽  
Degrading Bacteria ◽  
Petroleum Resources ◽  
Oil Concentration ◽  
American Society ◽  
Hydrocarbon Utilizing Bacteria

Aim: The research aims to assess the biodegradability of crude oil polluted aquatic environment using indigenous hydrocarbon degrading bacteria. Place and Duration of Study: The research was conducted in the Environmental Management and Toxicology Laboratory, Federal University of Petroleum Resources, Effurun, Delta State. Methodology: Hydrocarbon degrading bacteria species were isolated from hydrocarbon contaminated soils, screened and used for the degradation of crude oil. 5% and 10% crude oil were used to spike the test microcosm. Physicochemical parameters such as, pH, turbidity, total petroleum hydrocarbon (TPH) and bacterial counts of the bioremediated crude oil contaminated water were monitored on Day 0, 7 and 14. The biodegradation of the crude oil was done with the various bacteria isolates singly and as a consortium. Standard methods of American Public Health Association (APHA) and American Society for Testing and Materials (ASTM) were used for the analysis. Results: The isolates identified and used for the biodegradation process were, Azomonas sp., Enterococcus sp., Klebsiella sp. and Rhizobactersp. On day 14, in the microcosms with 5% crude oil contamination, Azomonas sp. recorded the highest turbidity reading of 328 ± 2.0 NTU, while Rhizobacter sp. recorded the least with 57.67 ± 0.58 NTU. The bacterial countswere between 7.68 ± 0.002 CFU/ml and 8.05 ± 0.10x 107 CFU/ml for Rhizobacter sp. and Azomonas sp. respectively.The crude oil was also degraded most in the microcosm treated with Azomonas sp. with a residual TPH concentration of 0.0013± 0.005 mg/l.For the 10% crude oil contaminated microcosms, TPH was also biodegraded most by Azomonas sp. with a value of 0.0026 ± 0.002mg/l. Turbidity readings were between 82 ± 1.0 NTU and 375.33 ± 0.57 NTU for Rhizobacter sp. and Azomonas sp. respectively. Bacterial counts were between (7.71± 0.012)x 107CFU/ml – (8.13± 0.001) x 107CFU/ml for Rhizobacter sp. and Azomonassp. respectively. Conclusion:There wasincreased microbial countsand decrease of residual crude oil concentration, indicating degradation of the crude oil by all the isolates.However, Azomonas sp. recorded the highest TPH degradation for both the 5% and 10% crude oil contaminated microcosms.Thus, findings from the research indicate that hydrocarbon degrading bacteria exist in our environment and can be used in the remediation of aquatic polluted environment.

Effects of Nutrient Source and Supply on Crude Oil Biodegradation in Continuous-Flow Beach Microcosms

2006 ◽  
Vol 132 (1) ◽  
pp. 75-84 ◽  
Author(s):  
Brian A. Wrenn ◽  
Kathryn L. Sarnecki ◽  
Eugene S. Kohar ◽  
Kenneth Lee ◽  
Albert D. Venosa
Keyword(s):  
Crude Oil ◽  
Continuous Flow ◽  
Nutrient Source ◽  
Oil Biodegradation
Sign in / Sign up

Export Citation Format

Share Document