Multiplex PCR for Rapid Detection of Rifampin and Isoniazid Resistance in Mycobacterium tuberculosis Isolated from Bandung, Indonesia

2007 ◽  
Vol 1 (3) ◽  
pp. 114-118 ◽  
Author(s):  
HERA NOVIANA ◽  
ZEILY NURACHMAN ◽  
MAELITA RAMDANI ◽  
AS NOER
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multiplex Pcr ◽  
Rapid Detection ◽  
Isoniazid Resistance

Improved real-time PCR for rapid detection of rifampin and isoniazid resistance in Mycobacterium tuberculosis clinical isolates

2003 ◽  
Vol 45 (3) ◽  
pp. 207-212 ◽  
Author(s):  
Maria J Torres ◽  
Antonio Criado ◽  
Maite Ruiz ◽  
Ana C Llanos ◽  
Jose C Palomares ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Clinical Isolates ◽  
Isoniazid Resistance

scholarly journals Rapid detection of Mycobacterium tuberculosis DNA and genetic markers for Isoniazid resistance in Ziehl-Neelsen stained slides

2020 ◽  
Vol 115 ◽  
Author(s):  
Graziele Lima Bello ◽  
Franciele Costa Leite Morais ◽  
Sheile Pinheiro de Jesus ◽  
Jonas Michel Wolf ◽  
Mirela Gehlen ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Genetic Markers ◽  
Rapid Detection ◽  
Isoniazid Resistance

scholarly journals New Multiplex PCR for Rapid Detection of Isoniazid-Resistant Mycobacterium tuberculosis Clinical Isolates

2005 ◽  
Vol 49 (1) ◽  
pp. 144-147 ◽  
Author(s):  
Laura Herrera-León ◽  
Tamara Molina ◽  
Pilar Saíz ◽  
Juan Antonio Sáez-Nieto ◽  
Maria Soledad Jiménez
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multiplex Pcr ◽  
High Frequency ◽  
Rapid Detection ◽  
Fragment Length Polymorphism ◽  
Regulatory Region ◽  
Clinical Isolates ◽  
Ribosome Binding ◽  
Frequent Mutations ◽  
Further Development

ABSTRACT In this study, we describe a multiplex PCR to detect a AGC→ACC (serine to threonine) mutation in the katG gene and a −15 C-to-T substitution (inhA C−15T) at the 5′ end of a presumed ribosome binding site in the promoter of the mabA-inhA operon. These mutations have been reported in the majority of previous studies as the most frequent mutations involved in the resistance to isoniazid (INH) of Mycobacterium tuberculosis clinical strains with high levels of resistance. The method was optimized and validated after an analysis of 30 M. tuberculosis clinical isolates with known sequences of the relevant part of the katG gene and the regulatory region of the mabA-inhA operon. We analyzed 297 INH-resistant M. tuberculosis isolates collected in Spain from 1996 to 2003 by PCR-restriction fragment length polymorphism (using the katG gene), DNA sequencing, and the newly developed multiplex PCR. The results were concordant for all 297 isolates tested. The analysis revealed that 204 (68.7%) of the isolates carried one or both of the mutations. This finding suggests that with further development this multiplex PCR will be able to detect the majority of the INH-resistant M. tuberculosis clinical isolates from Spain and other countries where a high frequency of similar mutations occur.

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