Simultaneous determination of diazepam，nordazepam and flumazenil in Beagle dog plasma by HPLC method

2015 ◽  
Vol 15 (2) ◽  
pp. 126-128
Author(s):  
WenQing LI ◽  
Hao ZOU ◽  
YanQiang ZHONG
Keyword(s):  
Simultaneous Determination ◽  
Hplc Method ◽  
Beagle Dog ◽  
Dog Plasma

scholarly journals Effect of Sijunzi Pills on Pharmacokinetics of Omeprazole in Beagle Dogs by HPLC-UV: A Herb-Drug Interaction Study

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Hua-ling Xia ◽  
Xin-jie Zhao ◽  
Yu-ji Zhang ◽  
Xiao-hang Su ◽  
Bo Sun ◽  
...  
Keyword(s):  
Internal Standard ◽  
Hplc Method ◽  
Array Detector ◽  
Pharmacokinetic Parameters ◽  
Beagle Dogs ◽  
Beagle Dog ◽  
Dog Plasma ◽  
Group A ◽  
Group B

A sensitive high-performance liquid chromatography (HPLC-UV) method for determination of omeprazole in beagle dog plasma was developed and to investigate the effect of Sijunzi pills (SJZPs) on the pharmacokinetics of omeprazole in beagle dogs. The beagle dog plasma was extracted with ethyl acetate and n-hexane under alkaline conditions. Omeprazole and internal standard (IS, fluconazole) were separated on an XDB-C18 column, and acetonitrile and 0.1% trifluoroacetic acid were used as the mobile phase. Omeprazole and IS were detected by using a diode array detector. This experiment adopts the experimental design of double-cycle self-control. In the first cycle (group A), six beagle dogs were given omeprazole 0.67 mg/kg orally in a single dose. In the second period (group B), the same six beagle dogs were orally given SJZPs 0.2 g/kg twice a day for 7 consecutive days, and then, omeprazole was orally given. At the different time points after omeprazole was given in the two periods, the blood samples were collected. The concentration of omeprazole was detected by the developed HPLC method. DAS 2.0 was used to calculate the pharmacokinetic parameters of omeprazole. Under the current experimental conditions, this UPLC method showed good linearity in the detection of omeprazole. Interday and intraday precision did not exceed 10%, and the range of accuracy values were from −1.43% to 2.76%. The results of extraction recovery and stability met the requirements of FDA approval guidelines of bioanalytical method validation. The Cmax of omeprazole in group B was 61.55% higher than that in group A, and the AUC(0−t) and AUC(0−∞) of omeprazole in group B were 63.96% and 63.65% higher those that in group A, respectively. At the same time, the clearance (CL) and apparent volume of distribution (Vd) decreased in group B. In this study, an HPLC method for the determination of plasma omeprazole concentration was established. SJZPs could inhibit the metabolism of omeprazole and increase the concentration of omeprazole in beagle dogs. It is suggested that when SJZPs are combined with omeprazole, attention should be paid to the herb-drug interactions and possible adverse reactions.

Determination of danshensu concentration in beagle dog plasma by on-line solid phase extraction-HPLC method

2011 ◽  
Vol 11 (3) ◽  
pp. 203-206
Author(s):  
Li WANG ◽  
XiaoRu CUI ◽  
YingHui DOU ◽  
Jun WEN ◽  
TaiJun HANG ◽  
...  
Keyword(s):  
Solid Phase Extraction ◽  
Solid Phase ◽  
Hplc Method ◽  
Phase Extraction ◽  
Beagle Dog ◽  
Dog Plasma ◽  
On Line
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