Cupric ions at high concentrations induce rigor-like contraction in a Ca2+-free medium in the guinea pig taenia coli

2008 ◽  
Vol 25 (07) ◽  
pp. 136-142
Author(s):  
T. Nasu ◽  
M. Nakagawa
Keyword(s):  
Guinea Pig ◽  
Taenia Coli ◽  
Free Medium ◽  
High Concentrations

The uptake and efflux of α-aminoisobutyric acid by the smooth muscle of the guinea pig's taenia coli

1979 ◽  
Vol 57 (10) ◽  
pp. 1114-1121
Author(s):  
J. H. Widdicombe ◽  
D. M. Paton
Keyword(s):  
Smooth Muscle ◽  
Guinea Pig ◽  
Effective Treatment ◽  
Taenia Coli ◽  
Free Medium ◽  
Efflux Rate ◽  
Aminoisobutyric Acid ◽  
High K ◽  
Treatment Changes ◽  
High Concentrations

The uptake of α-aminoisobutyric acid (AIB) by guinea pig taenia coli was linear with time over a 5-h period. There was a saturable component with Km of 0.7 mM and a maximal value of 4.75 mmol/kg tissue per hour. A second nonsaturable component of uptake became important at high concentrations of AIB (> 5 mM). Preincubation in K+-free or Na+-free (high-K+) media greatly reduced uptake. The dependence of AIB uptake on outside [Na+] ([Na+]0) was approximately linear over the range 0–140 mM. With [Na+]0 equal to 25 mM the uptakes with various substitutes were in the order sucrose > Mg2+ > choline+ > Li+ > Cs+ > Rb+ = K+. Addition of ouabain or removal of K+ during efflux of AIB led to marked increases in the efflux rate, ouabain being the more effective treatment. Changes in tissue Na+ and K+ levels were found to be slightly greater with ouabain than with K+-free medium. Lanthanum (5 mM) prevented the uptake of Na+ seen in K+-free medium and also abolished the increase in the rate of loss of AIB. It also reduced the rise in tissue Na+ produced by ouabain; there was a corresponding reduction in the ouabain-induced increase in the rate of loss of AIB. It is concluded that both the influx and efflux of AIB in this tissue are Na+ dependent, and that the accumulation of AIB relies on the transmembrane Na+ gradient.

The release of slow-reacting substance of anaphylaxis from guinea pig lung: effects of calcium antagonists

1985 ◽  
Vol 63 (1) ◽  
pp. 23-29 ◽  
Author(s):  
Melissa A. Damiano ◽  
Edward J. Barbieri
Keyword(s):  
Guinea Pig ◽  
Intracellular Calcium ◽  
Lung Tissue ◽  
Calcium Antagonists ◽  
Lung Parenchyma ◽  
Free Medium ◽  
Bicarbonate Buffer ◽  
Normal Medium ◽  
Voltage Dependent ◽  
High Concentrations

The effects of three calcium antagonists, verapamil, lanthanum, and 8-(N,N-diethylamino)octyl-3,4,5-trimethoxybenzoate (TMB-8) were studied on the release of slow-reacting substance of anaphylaxis (SRS-A) from ovalbumin-sensitized chopped guinea pig lung parenchyma in calcium-containing and calcium-free media. The SRS-A levels (mean ± SEM) obtained from tissues incubated in normal and calcium-free Krebs–bicarbonate buffer were 51 ± 8 (N = 19) and 21 ± 4 (N = 14) U/mL, respectively. TMB-8 (0.1–10 μM) a reported intracellular calcium antagonist, reduced antigen-stimulated SRS-A release from lung tissue incubated in calcium-containing, but not calcium-free, medium; A23187-induced SRS-A release from normal guinea pig lung was not significantly altered by TMB-8 at concentrations up to 10 μM. Verapamil and lanthanum consistently reduced SRS-A release only at high concentrations (100 μM and 1 mM, respectively). The quantities of SRS-A released from lung tissue incubated in the presence of verapamil in normal medium were similar to those obtained in calcium-free medium. Tissues incubated in the presence of potassium chloride (60 and 100 mM) did not release significant quantities of SRS-A, and release which did occur was not blocked by verapamil, suggesting that antigen-induced SRS-A release is not dependent on membrane depolarization and that verapamil was not exerting inhibition via blockade of voltage-dependent calcium channels. These data suggest that although intracellular calcium is important for the regulation of SRS-A secretion from guinea pig lung tissue, extracellular calcium is necessary for optimal release of SRS-A.

Ca45 uptake and tissue calcium in K-induced phasic and tonic contraction in taenia coli

1964 ◽  
Vol 207 (4) ◽  
pp. 873-876 ◽  
Author(s):  
N. Urakawa ◽  
W. C. Holland
Keyword(s):  
Guinea Pig ◽  
Muscle Contraction ◽  
Significant Rise ◽  
Tonic Contraction ◽  
Taenia Coli ◽  
Free Medium ◽  
Phasic Contraction ◽  
High K ◽  
Tissue Calcium ◽  
Phasic And Tonic Contraction

High-K medium produces a tonic contraction in guinea pig taenia coli. If muscle strips are preincubated in glucose-free medium, K produces only a phasic contraction. A comparison of Ca45 entry and tissue Ca changes in the two responses were made. Both responses are accompanied by an enhanced uptake of Ca45. In addition to an increased Ca45 uptake, a significant rise of tissue Ca was observed during the tonic contraction. No detectable changes in tissue Ca were noted in the phasic contraction. In light of modern theories of muscle contraction, it was proposed that in the phasic contraction, sufficient Ca is released from a cellular site to initiate contraction, whereas in the tonic contraction enough Ca crosses the membrane to initiate contraction. The transmembrane Ca transport involved in the latter response appeared to be dependent on metabolism.

Hepatic uptake of intact glutathione S-conjugate, inhibition by organic anions, and sinusoidal catabolism

1993 ◽  
Vol 265 (3) ◽  
pp. G547-G554
Author(s):  
C. A. Hinchman ◽  
A. T. Truong ◽  
N. Ballatori
Keyword(s):  
Guinea Pig ◽  
Rat Liver ◽  
Marked Decrease ◽  
Hepatic Uptake ◽  
Half Time ◽  
High Concentrations ◽  
Rat Livers ◽  
Dose Dependent ◽  
Uptake And Metabolism ◽  
Guinea Pig Liver

To identify potential mechanisms for hepatic removal of circulating glutathione (GSH) conjugates, uptake and metabolism of S-2,4-dinitrophenylglutathione (DNP-SG) were examined in isolated perfused livers from rat and guinea pig. Guinea pig livers perfused with 5 mumol of DNP-SG in a recirculating system (50 microM initial concn) rapidly cleared the conjugate from the perfusate (half time 3.7 min), whereas clearance was considerably slower in rat liver (half time 35 min). Disappearance of DNP-SG from the perfusate was accompanied by a simultaneous appearance of DNP-SG and its metabolites in bile. Addition of acivicin, an inhibitor of gamma-glutamyltransferase (gamma-GT), to the perfusate resulted in a marked decrease in DNP-SG clearance by guinea pig liver but had no effect in rat liver, suggesting that in the guinea pig this process is largely dependent on sinusoidal gamma-GT activity. However, even in the presence of acivicin, rat and guinea pig livers removed nearly one-half of the administered DNP-SG from the recirculating perfusate over 30 min. High concentrations of DNP-SG were found in bile (up to 3.7 mM), indicating that the liver is capable of transporting the intact conjugate from the circulation. When rat livers were perfused with higher concentrations of DNP-SG (100 and 250 microM), biliary excretion of DNP-SG increased dose dependently, with concentrations in bile reaching 10 mM at the higher dose. This was accompanied by a dose-dependent choleresis.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Inhibitory effect of beauvericin on a high K+-induced tonic contraction in guinea-pig taenia coli.

1987 ◽  
Vol 45 (3) ◽  
pp. 317-325 ◽  
Author(s):  
Shinjiro NAKAJYO ◽  
Kiyomi MATSUOKA ◽  
Tomohiro KITAYAMA ◽  
Yutaka YAMAMURA ◽  
Kazumasa SHIMIZU ◽  
...  
Keyword(s):  
Guinea Pig ◽  
Inhibitory Effect ◽  
Tonic Contraction ◽  
Taenia Coli ◽  
High K

scholarly journals EFFECT OF HISTAMINE ON OXYGEN CONSUMPTION IN GUINEA PIG TAENIA COLI

1971 ◽  
Vol 21 (5) ◽  
pp. 605-611
Author(s):  
Yukio SAITO ◽  
Yutaka SAKAI ◽  
Miyoshi IKEDA ◽  
Norimoto URAKAWA
Keyword(s):  
Oxygen Consumption ◽  
Guinea Pig ◽  
Taenia Coli

scholarly journals EFFECT OF HISTAMINE ON CALCIUM MOVEMENT IN GUINEA PIG TAENIA COLI

1971 ◽  
Vol 21 (5) ◽  
pp. 597-603 ◽  
Author(s):  
Tetsuyuki NASU ◽  
Hideaki KARAKI ◽  
Miyoshi IKEDA ◽  
Norimoto URAKAWA
Keyword(s):  
Guinea Pig ◽  
Taenia Coli
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