scholarly journals Clinical Neuropathology image 1-2018: Golgi silver staining, the black reaction

2018 ◽  
Vol 37 (01) ◽  
pp. 4-5
Author(s):  
Sara Mariotto ◽  
Marina Bentivoglio ◽  
Tiziana Cotrufo ◽  
Antonella Berzero ◽  
Salvatore Monaco ◽  
...  
Keyword(s):  
Silver Staining ◽  
Black Reaction

Silver Staining of Pancreatic Islets as Revealed by Electron Microscopy

1967 ◽  
Vol 25 ◽  
pp. 44-45
Author(s):  
Kazuaki Misugi ◽  
Nobuko Misugi ◽  
Hiroshi Yamada
Keyword(s):  
Pancreatic Islet ◽  
Silver Staining ◽  
Islet Cells ◽  
Severe Hypoglycemia ◽  
Granular Cell ◽  
Electron Microscopic Level ◽  
Staining Reaction ◽  
Electron Microscopic ◽  
Pancreatic Islet Cell ◽  
D Cell

The authors had described the fine structure of a type of pancreatic islet cell, which appeared different from typical alpha and beta cells, and tentatively considered that this third type of granular cell probably represents the D cell (Figure 1).Since silver staining has been widely used to differentiate different types of pancreatic islet cells by light microscopy, an attempt to examine this staining reaction at the electron microscopic level was made.Material and Method: Surgically removed specimens from three infants who suffered from severe hypoglycemia were used. The specimens were fixed and preserved in 20% neutral formalin. Frozen sections, 30 to 40 micron thick, were prepared and they were stained by Bielschowsky's method as modified by Suzuki (2). The stained sections were examined under a microscope and islet tissues were isolated. They were fixed in 1% osmium tetroxide in phosphate buffer for one hour and embedded in Epon 812 following dehydration through a series of alcohols and propylene oxide.

Detection of atrial natriuretic peptides (ANP) in rat atria by immunogold-silver staining (IGSS)

1994 ◽  
Vol 52 ◽  
pp. 304-305
Author(s):  
Robyn Rufner ◽  
Gerhard W. Hacker ◽  
Michele Forte ◽  
Nancyleigh E. Carson ◽  
Cristina Xenachis ◽  
...  
Keyword(s):  
Natriuretic Peptides ◽  
Silver Staining ◽  
Sodium Thiosulfate ◽  
Atrial Natriuretic Peptides ◽  
Paraffin Sections ◽  
Glass Slides ◽  
Metallic Salts ◽  
Wistar Kyoto ◽  
Right Atria

The use of immunogold-silver staining (IGSS) to enhance label penetration and Localization for immunocytochemistry or in situ hybridization utilizing a variety of metallic salts has been documented. In this morphological study, the effects of silver acetate, silver lactate and silver nitrate were evaluated for immunogold-labeling of a trial natriuretic peptides (ANP) in rat right atria.Eight Wistar Kyoto retired breeders were sedated with pentobarbital, perfused with either 4% paraformaldehyde (LM) or Karnovsky's fixative (EM), and right atria were dissected, processed, embedded in paraffin or epon, respectively and sectioned according to conventional methods. For light microscopy, an indirect IGSS method according to Hacker (3) was performed. Paraffin sections on glass slides were washed in ddH2O, immersed in Lugol's iodine, washed in ddH2O and treated with 2.5% aqueous sodium thiosulfate for 20 sec. After additional washes in ddH2O and TBS-0.1% fish gelatin, 10% normal goat serum (PBS with 1% BSA) was applied for 20 min before an overnight incubation at 4°C with a polyclonal α-ANP primary antibody (Peninsula Labs, 1:1000 in TBS/BSA).

Silver staining for proteins in SDS-polyacrylamide gel electrophoresis.

1986 ◽  
Vol 30 (3) ◽  
pp. 229-238 ◽  
Author(s):  
Tsutomu Inoue ◽  
Hiroaki Asaga ◽  
Mayumi Tamura
Keyword(s):  
Silver Staining ◽  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel

Visualizing Quantum Dots in Biological Samples Using Silver Staining

2009 ◽  
Vol 81 (11) ◽  
pp. 4560-4565 ◽  
Author(s):  
Leo Y. T. Chou ◽  
Hans C. Fischer ◽  
Steve D. Perrault ◽  
Warren C. W. Chan
Keyword(s):  
Quantum Dots ◽  
Biological Samples ◽  
Silver Staining

Silver staining of nucleic acids. Applications in virus research and in diagnostic virology

1983 ◽  
Vol 7 (4) ◽  
pp. 185-198 ◽  
Author(s):  
J.Lindsay Whitton ◽  
Fiona Hundley ◽  
Barbara O'donnell ◽  
Ulrich Desselberger
Keyword(s):  
Nucleic Acids ◽  
Silver Staining ◽  
Virus Research ◽  
Diagnostic Virology

A simplification of Heukeshoven and Dernick's silver staining of proteins

1987 ◽  
Vol 8 (3) ◽  
pp. 158-159 ◽  
Author(s):  
Catherine Damerval ◽  
Martine Le Guilloux ◽  
Joël Blaisonneau ◽  
Dominique de Vienne
Keyword(s):  
Silver Staining
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