DNA cleavage reactions of the dinuclear chemotherapeutic agent copper(II) bis-1,10- phenanthroline terephthalate

2012 ◽  
Vol 50 (01) ◽  
pp. 79-81 ◽  
Author(s):  
Andrew Kellett ◽  
Malachy McCann ◽  
Orla Howe ◽  
Mark O’Connor ◽  
Michael Devereux
Keyword(s):  
Dna Cleavage ◽  
Chemotherapeutic Agent ◽  
Cleavage Reactions

A New Method for Determining the Stereochemistry of DNA Cleavage Reactions:  Application to theSfiI andHpaII Restriction Endonucleases and to the MuA Transposase†

Biochemistry ◽  
1999 ◽  
Vol 38 (14) ◽  
pp. 4640-4648 ◽  
Author(s):  
Kiyoshi Mizuuchi ◽  
Timothy J. Nobbs ◽  
Stephen E. Halford ◽  
Kenji Adzuma ◽  
Jun Qin
Keyword(s):  
Dna Cleavage ◽  
Restriction Endonucleases ◽  
New Method ◽  
Cleavage Reactions

Novel molecular beacon assays for DNA cleavage reactions

2001 ◽  
Author(s):  
Jianwei J. Li ◽  
Charles Z. Cao ◽  
Ronald Geyer ◽  
Weihong Tan
Keyword(s):  
Dna Cleavage ◽  
Molecular Beacon ◽  
Cleavage Reactions

Hydroxylation, Epoxidation, and DNA Cleavage Reactions Mediated by the Biomimetic Mn-TMPyP/O2/Sulfite Oxidation System†

1999 ◽  
Vol 38 (18) ◽  
pp. 4123-4127 ◽  
Author(s):  
Karine Wietzerbin ◽  
James G. Muller ◽  
Rachel A. Jameton ◽  
Geneviève Pratviel ◽  
Jean Bernadou ◽  
...  
Keyword(s):  
Dna Cleavage ◽  
Sulfite Oxidation ◽  
Cleavage Reactions ◽  
Oxidation System

scholarly journals DNA Cleavage Activity of the V(D)J Recombination Protein RAG1 Is Autoregulated

2004 ◽  
Vol 24 (15) ◽  
pp. 6850-6860 ◽  
Author(s):  
Pallabi De ◽  
Mandy M. Peak ◽  
Karla K. Rodgers
Keyword(s):  
Dna Cleavage ◽  
Active Site Residues ◽  
Inhibitory Effects ◽  
Central Domain ◽  
Cleavage Activity ◽  
Dna Cleavage Activity ◽  
Recombination Protein ◽  
Cleavage Reactions ◽  
Hydrolysis Of ◽  
B And T Lymphocytes

ABSTRACT RAG1 and RAG2 catalyze the first DNA cleavage steps in V(D)J recombination. We demonstrate that the isolated central domain of RAG1 has inherent single-stranded (ss) DNA cleavage activity, which does not require, but is enhanced by, RAG2. The central domain, therefore, contains the active-site residues necessary to perform hydrolysis of the DNA phosphodiester backbone. Furthermore, the catalytic activity of this domain on ss DNA is abolished by addition of the C-terminal domain of RAG1. The inhibitory effects of this latter domain are suppressed on substrates containing double-stranded (ds) DNA. Together, the activities of the reconstituted domains on ss versus mixed ds-ss DNA approximate the activity of intact RAG1 in the presence of RAG2. We propose how the combined actions of the RAG1 domains may function in V(D)J recombination and also in aberrant cleavage reactions that may lead to genomic instability in B and T lymphocytes.

A Fluorometric Assay for DNA Cleavage Reactions Characterized with BamHI Restriction Endonuclease

1994 ◽  
Vol 220 (2) ◽  
pp. 377-383 ◽  
Author(s):  
S.P. Lee ◽  
D. Porter ◽  
J.G. Chirikjian ◽  
J.R. Knutson ◽  
M.K. Han
Keyword(s):  
Restriction Endonuclease ◽  
Dna Cleavage ◽  
Fluorometric Assay ◽  
Cleavage Reactions
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