Diagnostic value of electron microscopy detection using abdominal fat pad biopsy in systemic amyloidosis

2021 ◽  
Author(s):  
Di Wang ◽  
Yan Wang ◽  
Baojian Liu ◽  
Yingying Liu ◽  
Shiren Sun
Keyword(s):  
Electron Microscopy ◽  
Diagnostic Value ◽  
Abdominal Fat ◽  
Systemic Amyloidosis ◽  
Fat Pad

Diagnostic value of abdominal wall fat pad biopsy in senile systemic amyloidosis

Amyloid ◽  
2011 ◽  
Vol 18 (4) ◽  
pp. 211-215 ◽  
Author(s):  
Shu-ichi Ikeda ◽  
Yoshiki Sekijima ◽  
Kana Tojo ◽  
Jun Koyama
Keyword(s):  
Abdominal Wall ◽  
Diagnostic Value ◽  
Systemic Amyloidosis ◽  
Fat Pad ◽  
Senile Systemic Amyloidosis

Evaluation of Abdominal Fat Pad Aspiration Cytology and Grading for Detection in Systemic Amyloidosis

2007 ◽  
Vol 51 (6) ◽  
pp. 860-864 ◽  
Author(s):  
Sadhna Dhingra ◽  
Narendra Krishnani ◽  
Niraj Kumari ◽  
Rakesh Pandey
Keyword(s):  
Abdominal Fat ◽  
Systemic Amyloidosis ◽  
Fat Pad ◽  
Aspiration Cytology

scholarly journals Diagnosis of Senile Amyloidosis by Fine Needle Aspiration Biopsy and Cell Block from Abdominal Fat Pad

2021 ◽  
pp. 84-90
Author(s):  
Arvind Bhake ◽  
Kaustubh Kharche
Keyword(s):  
Fine Needle Aspiration ◽  
Fine Needle Aspiration Cytology ◽  
Needle Aspiration ◽  
Abdominal Fat ◽  
Systemic Amyloidosis ◽  
Fat Pad ◽  
Aspiration Cytology ◽  
Fine Needle ◽  
Senile Systemic Amyloidosis ◽  
Needle Aspiration Cytology

Background:  Amyloidosis is menacingly increasing medical problem in aging population across globe. Its clinical presentation is varied. Its laboratory diagnosis often requires biopsy material. The fine needle aspiration cytology (FNAC, FNAB) offers dependable diagnostic alternative to cumbersome biopsy. Present protocol describes the FNAC of abdominal pad of fat as surrogate site for diagnosis of senile systemic amyloidosis avoiding biopsy other known sites.  Aim: To study diagnostic accuracy of subcutaneous abdominal fat tissue fine needle aspiration cytology/biopsy for detecting systemic (senile) amyloidosis and its utility in clinical practice. Objectives: To diagnose systemic amyloidosis (senile) by fine needle aspiration cytology/biopsy of abdominal fat pad on crush smears of aspirates and cell blocks. To correlate the diagnosis of deposits of amyloid with the clinical manifestations in specific and non specific clinical manifestations of amyloidosis. To know the sensitivity and specificity of the diagnosis of amyloid deposition on fine needle aspiration cytology/biopsy of abdominal fat pad in comparison to the results of cell block preparations. Methodology: A prospective study carried out to compare results of FNAC of abdominal pad of fat with paraffin embedded cell block in diagnosis of amyloidosis in suspected cases of cases of senile systemic amyloidosis. Technical adopted methods to be used are regular tissue stains, congo red stain and polarized microscopy.  Expected Results: It is expected that results of study will establish FNAC of abdominal pad of fat as dependable office diagnostic procedure that would avoid complicated biopsy procedures of gastrointestinal tissue, skin, bone marrow and others for senile systemic amyloidosis.  

scholarly journals Detection of amyloid in abdominal fat pad aspirates in early amyloidosis: Role of electron microscopy and Congo red stained cell block sections

CytoJournal ◽  
2011 ◽  
Vol 8 ◽  
pp. 11 ◽  
Author(s):  
Sumana Devata ◽  
Parameswaran Hari ◽  
Natalia Markelova ◽  
Rongshan Li ◽  
Richard Komorowski ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Blood Vessels ◽  
Congo Red ◽  
False Negative ◽  
False Negative Rate ◽  
Abdominal Fat ◽  
Fat Pad ◽  
Early Disease ◽  
Polarizing Microscopy ◽  
Negative Results

Background: Fine-needle aspiration biopsy (FNA) of the abdominal fat pad is a minimally invasive procedure to demonstrate tissue deposits of amyloid. However, protocols to evaluate amyloid in fat pad aspirates are not standardized, especially for detecting scant amyloid in early disease. Materials and Methods: We studied abdominal fat pad aspirates from 33 randomly selected patients in whom subsequent tissue biopsy, autopsy, and/or medical history for confirmation of amyloidosis (AL) were also available. All these cases were suspected to have early AL, but had negative results on abdominal fat pad aspirates evaluated by polarizing microscopy of Congo Red stained sections (CRPM). The results with CRPM between four reviewers were compared in 12 cases for studying inter observer reproducibility. 24 cases were also evaluated by ultrastructural study with electron microscopy (EM). Results: Nine of thirty-three (27%) cases reported negative by polarizing microscopy had amyloidosis. Reanalysis of 12 mixed positive-negative cases, showed considerable inter-observer variability with frequent lack of agreement between four observers by CRPM alone (Cohen's Kappa index of 0.1, 95% CI -0.1 to 0.36). EM showed amyloid in the walls of small blood vessels in fibroadipose tissue in four out of nine cases (44%) with amyloidosis. Conclusion: In addition to poor inter-observer reproducibility, CRPM alone in cases with scant amyloid led to frequent false negative results (9 out of 9, 100%). For improved detection of AL, routine ultrastructural evaluation with EM of fat pad aspirates by evaluating at least 15 small blood vessels in the aspirated fibroadipose tissue is recommended. Given the high false negative rate for CRPM alone in early disease, routine reflex evaluation with EM is highly recommended to avert the invasive option of biopsying various organs in cases with high clinical suspicion for AL.

Abdominal fat pad excisional biopsy for the diagnosis and typing of systemic amyloidosis

2018 ◽  
Vol 72 ◽  
pp. 71-79 ◽  
Author(s):  
Yessica Garcia ◽  
A. Bernard Collins ◽  
James R. Stone
Keyword(s):  
Excisional Biopsy ◽  
Abdominal Fat ◽  
Systemic Amyloidosis ◽  
Fat Pad

Sites of Insulin Action Using Ferritin Labeling

1971 ◽  
Vol 29 ◽  
pp. 540-541
Author(s):  
Burton B. Silver ◽  
Ronald S. Nelson
Keyword(s):  
Electron Microscopy ◽  
Binding Sites ◽  
Anterior Pituitary ◽  
Insulin Action ◽  
Diabetic Rats ◽  
Insulin Antibody ◽  
Fat Pad ◽  
Target Organs ◽  
Uranium Salts ◽  
Sugar Levels

Some investigators feel that insulin does not enter cells but exerts its influence in some manner on the cell surface. Ferritin labeling of insulin and insulin antibody was used to determine if binding sites of insulin to specific target organs could be seen with electron microscopy.Alloxanized rats were considered diabetic if blood sugar levels were in excess of 300 mg %. Test reagents included ferritin, ferritin labeled insulin, and ferritin labeled insulin antibody. Target organs examined were were diaphragm, kidney, gastrocnemius, fat pad, liver and anterior pituitary. Reagents were administered through the left common carotid. Survival time was at least one hour in test animals. Tissue incubation studies were also done in normal as well as diabetic rats. Specimens were fixed in gluteraldehyde and osmium followed by staining with lead and uranium salts. Some tissues were not stained.

scholarly journals The Continuing Need for Electron Microscopy in Examination of Medical Renal Biopsies: Examples in Practice

2021 ◽  
pp. 1-15
Author(s):  
Michifumi Yamashita ◽  
Mercury Y. Lin ◽  
Jean Hou ◽  
Kevin Y.M. Ren ◽  
Mark Haas
Keyword(s):  
Electron Microscopy ◽  
Renal Biopsy ◽  
Renal Allograft ◽  
Comprehensive Evaluation ◽  
Diagnostic Value ◽  
Ultrastructural Studies ◽  
The Past ◽  
Biopsy Diagnosis ◽  
Renal Biopsies ◽  
Clinical Consequences

<b><i>Background:</i></b> For the better part of the past 6 decades, transmission electron microscopy (EM), together with routine light microscopy and immunofluorescence and/or immunohistochemistry (IHC), has been an essential component of the diagnostic workup of medical renal biopsies, particularly native renal biopsies, with increasing frequency in renal allograft biopsies as well. Studies performed prior to the year 2000 have indeed shown that a substantial fraction of renal biopsies cannot be accurately diagnosed without EM. Still, EM remains costly and labor-intensive, and with increasing pressure to reduce healthcare costs, some centers are de-emphasizing diagnostic EM. This trend has been coupled with advances in IHC and other methods in renal biopsy diagnosis over the past 2–3 decades. <b><i>Summary:</i></b> Nonetheless, it has been our experience that the diagnostic value of EM in the comprehensive evaluation of renal biopsies remains similar to what it was 20–30 years ago. In this review, we provide several key examples from our practice where EM was essential in making the correct renal biopsy diagnosis, ranging from relatively common glomerular lesions to rare diseases. <b><i>Key Messages:</i></b> EM remains an important component of the diagnostic evaluation of medical renal biopsies. Failure to perform EM in certain cases will result in an incorrect diagnosis, with possible clinical consequences. We strongly recommend that tissue for EM be taken and stored in an appropriate fixative and ultrastructural studies be performed for all native renal biopsies, as well as appropriate renal allograft biopsies as recommended by the Banff consortium.

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