scholarly journals Induction of Defense-Related Enzymes in Banana Plants: Effect of Live and Dead Pathogenic Strain of Fusarium oxysporum f. sp. cubense

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
Janki N. Thakker ◽  
Samiksha Patel ◽  
Pinakin C. Dhandhukia
Keyword(s):  
Enzyme Activity ◽  
Fusarium Oxysporum ◽  
Defense Response ◽  
Biocontrol Agent ◽  
Potential Candidate ◽  
Marker Enzyme ◽  
Panama Disease ◽  
Disease Response ◽  
Subsequent Exposure ◽  
Plant Vaccine

The aim of the present study was to scrutinize the response of banana (Grand Naine variety) plants when interacting with dead or live pathogen, Fusarium oxysporum f.sp. cubense, a causative agent of Panama disease. Response of plants was evaluated in terms of induction of defense-related marker enzyme activity, namely, peroxidase (POX), polyphenol oxidase (PPO), -1,3 glucanase, chitinase, and phenolics. Plant's interaction with live pathogen resulted in early induction of defense to restrain penetration as well as antimicrobial productions. However, pathogen overcame the defense of plant and caused disease. Interaction with dead pathogen resulted in escalating defense response in plants. Later on plants inoculated with dead pathogen showed resistance to even forced inoculation of live pathogen. Results obtained in the present study suggest that dead pathogen was able to mount defense response in plants and provide resistance to Panama disease upon subsequent exposure. Therefore, preparation from dead pathogen could be a potential candidate as a biocontrol agent or plant vaccine to combat Panama disease.

scholarly journals Complete Genome Sequence of Ralstonia solanacearum FJAT-1458, a Potential Biocontrol Agent for Tomato Wilt

2017 ◽  
Vol 5 (14) ◽  
Author(s):  
Deju Chen ◽  
Bo Liu ◽  
Yujing Zhu ◽  
Jieping Wang ◽  
Zheng Chen ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Bacterial Wilt ◽  
Ralstonia Solanacearum ◽  
Complete Genome Sequence ◽  
Biocontrol Agent ◽  
Avirulent Strain ◽  
Potential Candidate ◽  
Content Type ◽  
Potential Biocontrol Agent ◽  
Plant Vaccine

ABSTRACT An avirulent strain of Ralstonia solanacearum FJAT-1458 was isolated from a living tomato. Here, we report the complete R. solanacearum FJAT-1458 genome sequence of 6,059,899 bp and 5,241 genes. This bacterial strain is a potential candidate as a biocontrol agent in the form of a plant vaccine for bacterial wilt.

scholarly journals Characterization of Maize Genotypes (Zea mays L.) for Resistance to Striga asiatica and S. hermonthica and Compatibility with Fusarium oxysporum f. sp. strigae (FOS) in Tanzania

Agronomy ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1004
Author(s):  
John Lobulu ◽  
Hussein Shimelis ◽  
Mark D. Laing ◽  
Arnold Angelo Mushongi ◽  
Admire Isaac Tichafa Shayanowako
Keyword(s):  
Grain Yield ◽  
Fusarium Oxysporum ◽  
Biocontrol Agent ◽  
Zea Mays L ◽  
Control Treatment ◽  
Yield Reduction ◽  
Maize Genotypes ◽  
Significant Yield ◽  
The Mean

Striga species cause significant yield loss in maize varying from 20 to 100%. The aim of the present study was to screen and identify maize genotypes with partial resistance to S. hermonthica (Sh) and S. asiatica (Sa) and compatible with Fusarium oxysporum f. sp. strigae (FOS), a biocontrol agent. Fifty-six maize genotypes were evaluated for resistance to Sh and Sa, and FOS compatibility. Results showed that FOS treatment significantly (p < 0.001) enhanced Striga management compared to the untreated control under both Sh and Sa infestations. The mean grain yield was reduced by 19.13% in FOS-untreated genotypes compared with a loss of 13.94% in the same genotypes treated with FOS under Sh infestation. Likewise, under Sa infestation, FOS-treated genotypes had a mean grain yield reduction of 18% while untreated genotypes had a mean loss of 21.4% compared to the control treatment. Overall, based on Striga emergence count, Striga host damage rating, grain yield and FOS compatibility, under Sh and Sa infestations, 23 maize genotypes carrying farmer preferred traits were identified. The genotypes are useful genetic materials in the development of Striga-resistant cultivars in Tanzania and related agro-ecologies.

scholarly journals Unraveling the Metabolite Signature of Endophytic Bacillus velezensis Strain Showing Defense Response Towards Fusarium oxysporum

Agronomy ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 683
Author(s):  
Yang Cao ◽  
Wei Ding ◽  
Chang Liu
Keyword(s):  
Fusarium Oxysporum ◽  
Inhibitory Activity ◽  
Defense Response ◽  
Endophytic Bacteria ◽  
Rice Seedling ◽  
Seedling Blight ◽  
Bacillus Velezensis ◽  
Secondary Bile Acid ◽  
Pathogen Inhibition

Seedling blight, caused by the fungus Fusarium oxysporum, significantly lowers rice production globally. Earlier reports have opined that endophytic bacteria strains could be possible biocontrol agents, but the mechanistic actions involved are still unclear. Therefore, this study aimed to isolate the endophytic bacteria with high inhibitory activity and elucidate its possible mechanisms for inducing resistance by metabolomics. The results showed that mdj-36 had the strongest in vitro pathogen inhibition of F. oxysporum, while mdj-34 displayed the lowest inhibitory activity identified as Bacillus velezensis strains. Metabolomic analyses demonstrated that B. velezensis mdj-36 growth medium could produce higher organic acids, terpenes, and diterpene than B. velezensis mdj-34. Further investigation revealed that ‘secondary bile acid biosynthesis’ and ‘glycerophospholipid metabolism’ pathways played essential roles in defense response towards F. oxysporum. This study’s findings provide a credible theoretical basis for the possible use of the B. velezensis strain against rice seedling blight.

scholarly journals REACTION OF BANANA CULTIVARS TO THE Meloidogyne javanica X Fusarium oxysporum f. sp. cubense COMPLEX

2018 ◽  
Vol 31 (3) ◽  
pp. 572-583
Author(s):  
LEANDRO DE SOUZA ROCHA ◽  
ROBERT FELIX DE SANTANA ◽  
ANA CRISTINA FERMINO SOARES ◽  
FERNANDO HADDAD
Keyword(s):  
Genetic Variability ◽  
Fusarium Oxysporum ◽  
Block Design ◽  
Meloidogyne Javanica ◽  
Simultaneous Presence ◽  
Sterile Soil ◽  
Panama Disease ◽  
Randomized Block Design ◽  
Resistant Varieties ◽  
Inoculation Time

ABSTRACT The interaction Fusarium oxysporum f. sp. cubense (Foc) x nematode and the genetic variability of Foc are the main problems with potential to affect the use of resistant varieties in the management of the Panama disease. The objective of this work was to evaluate the interaction between Foc and Meloidogyne javanica on the banana of the Prata Anã, Grande Naine and BRS Princesa cultivars. The experiment was conducted in a randomized block design, with six treatments and ten replications, which differed in the inoculation time of each pathogen. Simultaneous inoculation with Foc and M. javanica, inoculation with Foc one week before inoculation with M. javanica, and inoculation with M. javanica one week before inoculation with Foc. In addition to the controls, Foc isolated, M. javanica isolated, and cultivars without the pathogens. The seedlings were transplanted in 3-liter pots with sterile soil infested with 40 grams of Foc inoculum at the concentration of 1x106CFU/gram and 1000 eggs and J2 of M. javanica. At the end of 56 days, the treatments with 'Grande Naine' presented the highest reproduction factors of M. javanica. In the 'Prata Anã' no interaction was observed between Foc x M. javanica. The treatments with simultaneous inoculation of the two pathogens, and with Foc a week before, in the 'BRS Princesa', presented the highest external disease indices (DI), which promoted the highest AUDPC. The highest internal DI were observed in the treatments Foc+Mj+Grande Naine, Foc+Mj+BRS Princesa, Mj+Grande Naine+Foc and Foc+BRS Princesa+Mj. The simultaneous presence of M. javanica and Foc increases the severity of the Panama disease in Grande Naine and BRS Princesa cultivars.

scholarly journals ISOLATION OF ANTIFUNGI BACTERIA FROM BANANA RHIZOSPHERE TO INHIBIT Fusarium Oxysproum f.sp cubense (FOC) GROWTH

2019 ◽  
Vol 5 (3) ◽  
pp. 105
Author(s):  
Albert Sembiring ◽  
Natalia Lusianingsih Sumanto
Keyword(s):  
Fungal Pathogen ◽  
Room Temperature ◽  
Biocontrol Agent ◽  
Vascular Tissue ◽  
Agar Medium ◽  
Wilt Disease ◽  
In Vitro Test ◽  
Panama Disease ◽  
Vitro Test

Fusarium wilt disease on banana has been known as panama disease one of the main diseases that cause huge losses for banana farmers. It is caused by the soil-borne fungal pathogen, Fusarium oxysporum f.sp cubense (Foc), which is very hard control because it is saprophytic in the soil. The mold infiltrates the root to vascular tissue that induces yellowing on the leaf, so this pathogen can attack the root, stem dan leaf. The research aimed to search bacteria from the banana rhizosphere that have an antifungal activity to inhibit Foc growth. Bacteria was isolated by serial dilution then was spread on King’s B agar medium incubation 28oC (room temperature). Four quadrants in vitro test on PDA medium used twenty bacterial from isolation, from the test was obtained six isolates have the potential to inhibit the growth of Foc. Based on percentage inhibition radial growth four isolates that have inhibition 50% over which TR2 was the highest at 79.07%. The in vitro test confirmed that bacteria from the banana rhizosphere have potential as biocontrol agent because it was able to inhibit the Foc growth.

scholarly journals Evaluation of the Efficacy of Commercial Disinfectants Against Fusarium oxysporum f. sp. cubense Race 1 and Tropical Race 4 Propagules

Plant Disease ◽  
2019 ◽  
Vol 103 (4) ◽  
pp. 721-728 ◽  
Author(s):  
T. V. Nguyen ◽  
L. T. T. Tran-Nguyen ◽  
C. L. Wright ◽  
P. Trevorrow ◽  
K. Grice
Keyword(s):  
Fusarium Oxysporum ◽  
Sodium Hypochlorite ◽  
Active Ingredient ◽  
Panama Disease ◽  
Banana Production ◽  
Race 1 ◽  
Race 4 ◽  
On Farm ◽  
Farm Biosecurity ◽  
Ammonium Compounds

Panama disease caused by Fusarium oxysporum f. sp. cubense has devastated banana production worldwide. This work aimed to determine effective disinfectants against two races of F. oxysporum f. sp. cubense, race 1 and tropical race 4 (TR4), for implementation with on-farm biosecurity procedures against this disease following the outbreak of TR4 in North Queensland in 2015. A total of 32 commercial disinfectants were screened and their activity was assessed after ≤30 s, 5 min, 30 min, and 24 h of contact with an F. oxysporum f. sp. cubense suspension containing 105 chlamydospores/ml without and with soil added (0.05 g/ml). Of the disinfectants tested, the quaternary ammonium compounds containing ≥10% active ingredient were found to be the most effective against both F. oxysporum f. sp. cubense races. These products, when used at a 1:100 dilution, completely inhibited the survival of all F. oxysporum f. sp. cubense propagules across all the contact times regardless of the absence or presence of soil. The bioflavonoid product EvoTech 213 and bleach (10% sodium hypochlorite) used at a 1:10 dilution also eliminated all F. oxysporum f. sp. cubense propagules across all the contact times. None of the detergent-based or miscellaneous products tested were completely effective against both F. oxysporum f. sp. cubense races even used at a 1:10 dilution. Soil decreases the efficacy of disinfectants and therefore must be removed from contaminated items before treatments are applied.

Fusarium oxysporum f.sp. cubense. [Descriptions of Fungi and Bacteria].

1970 ◽  
Author(s):  
C. V. Subramanian
Keyword(s):  
Fusarium Oxysporum ◽  
Geographical Distribution ◽  
Infected Leaf ◽  
Vascular Wilt ◽  
Panama Disease ◽  
Alternative Hosts ◽  
Flood Water ◽  
Source Of Infection ◽  
Planting Material ◽  
Banana Wilt

Abstract A description is provided for Fusarium oxysporum f.sp. cubense. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On cultivated banana and abaca (Musa spp.) and wild spp. of Heliconia (Musaceae), esp. H. caribaea (42: 80); three species of grass (Paspalum fasciculatum, Panicum purpurascens andlxophorus unisetus), and Commelina diffusa (Commelinaceae) may serve as alternative hosts (Waite & Dunlap, 1953). DISEASES: Panama disease (vascular wilt) of banana (banana wilt) and vascular wilt in abaca (Musa textilis). GEOGRAPHICAL DISTRIBUTION: Widespread: Africa, Asia, Australasia and Oceania, C. America and W. Indies, N. & S. America (CMI Map 31). TRANSMISSION: Primarily soil-borne; possibly air-borne also. Pathogen may spread in infected leaf trash or soil and debris in surface flood-water. Infected planting material is another source of infection and spread.

NEAR-WILT OF PEAS IN ONTARIO

1959 ◽  
Vol 39 (4) ◽  
pp. 483-490
Author(s):  
Blair H. MacNeill ◽  
Helen Howard
Keyword(s):  
Fusarium Oxysporum ◽  
Wilt Disease ◽  
Disease Response ◽  
New Era ◽  
Host Parasite Relationship ◽  
Parasite Relationship ◽  
Race 2 ◽  
Host Parasite

It has been found that the near-wilt disease of peas, caused by Fusarium oxysporum f. pisi race 2 (Linford) Snyder & Hansen, is now present in the canning districts of Ontario. Conclusions as to the identity of the fungus associated with the wilting condition in field plants have been based upon a study of the differential disease-response of the pea varieties Little Marvel, W.R. Perfection and New Era, the histology of infection in the susceptible pea host, and the characteristics of the fungus when in culture. The capacity of the fungus to establish a symptomless host-parasite relationship with certain legumes not normally regarded as susceptible to the near-wilt pathogen has been demonstrated. The possible role of such "non-susceptible hosts" in perpetuating the fungus between pea crops has been discussed.

Non-pathogenic Fusarium oxysporum as a biocontrol agent

2020 ◽  
Vol 73 (2) ◽  
pp. 177-183
Author(s):  
A. Sajeena ◽  
Deepthi S. Nair ◽  
K. Sreepavan
Keyword(s):  
Fusarium Oxysporum ◽  
Biocontrol Agent

Mycoparasitic Trichoderma viride as a biocontrol agent against Fusarium oxysporum f. sp. adzuki and Pythium arrhenomanes and as a growth promoter of soybean

2010 ◽  
Vol 29 (12) ◽  
pp. 1452-1459 ◽  
Author(s):  
Rojan P. John ◽  
R.D. Tyagi ◽  
D. Prévost ◽  
Satinder K. Brar ◽  
Stéphan Pouleur ◽  
...  
Keyword(s):  
Fusarium Oxysporum ◽  
Biocontrol Agent ◽  
Trichoderma Viride ◽  
Growth Promoter ◽  
Pythium Arrhenomanes
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