scholarly journals An Isocratic Method for Quantification of Valproic Acid and Its Related Impurities Using Ion Pair Reagent by Ultraperformance Liquid Chromatography

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Rakshit Thakkar ◽  
Hitesh Saravaia ◽  
Mrunal Ambasana ◽  
Madhavi Patel ◽  
Anamik Shah
Keyword(s):  
Valproic Acid ◽  
Mobile Phase ◽  
Reversed Phase ◽  
Ion Pair ◽  
Related Impurities ◽  
Acid Sodium Salt ◽  
System Suitability ◽  
Liquid Chromatographic ◽  
Salt Flow

A selective ultraperformance liquid chromatographic (UPLC) method for the quantification of valproic acid and its known related impurities using ion pair reagent has been developed. The method includes reversed-phase Acquity HSS T3 column with 100 mm × 2.1 mm i.d. and 1.7 μ particle size. The mobile phase consists of acetonitrile, 5 mM 1-hexanesulphonic acid sodium salt, flow rate is 0.6 mL/min, and UV detection is performed at 215 nm. A system suitability test (SST) was developed to govern the quality of the separation. The developed method has been validated further with respect to linearity, accuracy, precision, selectivity, LOD, LOQ, and Robustness.

scholarly journals Determination of Magnesium Valproate and Its Process Related Impurities by Ultraperformance Liquid Chromatography

2014 ◽  
Vol 2014 ◽  
pp. 1-6
Author(s):  
Rakshit Thakkar ◽  
Hitesh Saravaia ◽  
Madhavi Patel ◽  
Anamik Shah
Keyword(s):  
Mobile Phase ◽  
Reversed Phase ◽  
Isocratic Elution ◽  
Related Impurities ◽  
System Suitability ◽  
Liquid Chromatographic ◽  
Ammonium Dihydrogen Orthophosphate ◽  
Dihydrogen Orthophosphate

A selective ultraperformance liquid chromatographic (UPLC) method for the determination of magnesium valproate and its process related impurities has been developed. The method includes reversed-phase Acquity BEH C18 column with 100 mm × 2.1 mm i.d. and 1.7 µ particle size. The mobile phase consists of acetonitrile and 5 mM ammonium dihydrogen orthophosphate with pH = 3.0 at 45 : 55 isocratic elution. The flow rate was set at 0.3 mL/min and UV detection was performed at 215 nm. A system suitability test (SST) was developed to govern the quality of the separation. The developed method has been validated further with respect to linearity, accuracy, precision, selectivity, LOD, LOQ, and robustness. Different batches of samples were examined using this method; the method proved to be successful when applied to analyze a marketed magnesium valproate formulation.

scholarly journals Liquid Chromatographic Determination of Scopolamine, Hyoscyamine, and Phenobarbital in Tablets

1997 ◽  
Vol 80 (2) ◽  
pp. 331-334 ◽  
Author(s):  
Susan Ting
Keyword(s):  
Mobile Phase ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Scopolamine Hydrobromide ◽  
Acid Sodium Salt ◽  
Liquid Chromatographic Method ◽  
Relative Standard ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic

Abstract A liquid chromatographic method using a reversed- phase C18 column and octanesulfonic acid sodium salt-methanol as the mobile phase was developed for the simultaneous determination of phenobarbi- tal, scopolamine, and hyoscyamine in tablets. The mixture of the 3 drugs was resolved in <8 min. Detector responses were linear for 10 μL injections of the following: scopolamine hydrobromide, 8.25-206.3 μg/mL; hyoscyamine sulfate, 15.01-750.76 μg/mL; and phenobarbital, 250-751 μg/mL. Recoveries from tablets were 100.8% for scopolamine hydrobromide, 100.1% for hyoscyamine sulfate, and 100.3% for phenobarbital. Replicate injections of scopolamine hydrobromide, hyoscyamine sulfate, and phenobarbital gave an overall relative standard deviation of <1.0% (n = 10). The method detected as little as 3.3 ng scopolamine hydrobromide.

Reversed-phase ion-pair chromatography with UV-absorbing ions in the mobile phase

1981 ◽  
Vol 218 ◽  
pp. 31-43 ◽  
Author(s):  
M. Denkert ◽  
L. Hackzell ◽  
G. Schill ◽  
E. Sjögren
Keyword(s):  
Mobile Phase ◽  
Reversed Phase ◽  
Ion Pair ◽  
Ion Pair Chromatography
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