scholarly journals Comparative Estimation of Major Iridoid Glucosides from Different Parts of Incarvillea emodi

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Ajay Rana ◽  
Harsh Pratap Singh ◽  
Devendra Dhyani
Keyword(s):  
Calibration Curve ◽  
Limit Of Detection ◽  
Dry Weight ◽  
Weight Basis ◽  
Rich Source ◽  
Linear Calibration ◽  
Limit Of Quantification ◽  
Complete Dominance ◽  
Iridoid Glucosides ◽  
Different Parts

Incarvillea emodi (Bignoniaceae) is a rich source of bioactive iridoid glucosides. This plant contains two major iridoid glucosides: plantarenaloside, a neurotropic compound, and boschnaloside, a strong antibacterial compound. Here, in this study we have developed a simple and fast HPLC-DAD method for the total comparative estimation of these two major iridoids from different parts of Incarvillea emodi. A linear calibration curve (r2=0.999) for both iridoid glucosides in varying range (15.6–500 μg/ml) is obtained. The limit of detection (LOD) and limit of quantification (LOQ) for plantarenaloside were 11.4 ng and 38 ng and for boschnaloside were 22.8 ng and 76 ng, respectively. The shoots, roots, and flowers of Incarvillea emodi have a combined presence of 7.66, 1.22, and 6.99 percent of these iridoid glucosides on dry weight basis. In shoots, plantarenaloside shows complete dominance (6.78%) over boschnaloside (0.88%), and a reversal of this trend was observed in case of flowers where boschnaloside shows complete dominance (6.12%) over plantarenaloside (0.87%). The roots contain 1.19% and 0.03% of both iridoids, respectively.

scholarly journals DEVELOPMENT AND EVALUATION OF NOVEL ESTIMATION TECHNIQUES FOR IN VITRO DISSOLUTION STUDY AND VALIDATION PROTOCOL FOR ESCITALOPRAM AS ANTIDEPRESSANT DRUG AND THEIR FORMULATION

2020 ◽  
pp. 55-61
Author(s):  
SHRIRAM H. BAIRAGI ◽  
R. S. GHOSH
Keyword(s):  
Limit Of Detection ◽  
Antidepressant Drug ◽  
Correlation Factor ◽  
In Vitro Dissolution ◽  
Linear Calibration ◽  
Limit Of Quantification ◽  
Minimum Concentration ◽  
Dissolution Study ◽  
Relative Standard

Objective: To develop and validate the RP-HPLC method and in vitro dissolution study for escitalopram as antidepressant drug and their formulation. Methods: The chromatographic separation was done by using a C-18, 150 mm column and a mobile phase consisting of phosphate buffer (40%) and acetonitrile HPLC grade (60%). Detection was carried out at 211 nm with a flow rate of 1 ml/min with an injection of 20 μl. The method was validated with different parameters such as linearity, precision, accuracy, robustness, and limit of detection (LOD), the limit of quantification (LOQ) according to ICH guidelines. Results: The linear calibration curve was obtained in the concentration range of 0-50 μg/ml and gave an average correlation factor 0.992. The retention time was observed at 2.96 min. The Minimum concentration level at which the analyte can be reliably detected (LOD) and quantified (LOQ) were found to be 0.03 and 0.09 µg/ml, respectively. The relative standard deviation of intra and the inter-day assay was found to be less than 2. The dissolution studies show moderate dissolution (23.4%) after 45 min, but it reaches a plateau after approximately 25 min. Conclusion: This method was found to be simple, rapid and economic with less run time. The validated parameters manifest the method is reliable, linear, accurate and precise as well as robust with minor variations in chromatographic parameters. Therefore, the developed method can be applied for both routine analysis and quality control assay and it could be a very powerful tool to investigate the stability of escitalopram.

scholarly journals Ultraviolet Spectrophotometric Method for Determination of Glipizide in Presence of Liposomal/Proliposomal Turbidity

2013 ◽  
Vol 2013 ◽  
pp. 1-5
Author(s):  
Neelkant Prasad ◽  
Roshan Issarani ◽  
Badri Prakash Nagori
Keyword(s):  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Quantitative Estimation ◽  
Uv Detection ◽  
Limit Of Quantification ◽  
Relative Standard ◽  
Precision And Accuracy ◽  
Clear Solution

A simple and sensitive ultraviolet spectrophotometric method for quantitative estimation of glipizide in presence of lipid turbidity is described to avoid false estimation due to diffraction by turbidity. UV detection was performed at 230 nm, 225 nm, and 235 nm, and the calibration curve was plotted between resultant of absorbance of [230 nm − (225 nm + 235 nm)/2] and concentration of analyte. The calibration curve was linear over the concentration range tested (1–20 μg/mL) with limit of detection of 0.27 μg/mL and limit of quantification of 0.82 μg/mL. Percent relative standard deviations and percent relative mean error, representing precision and accuracy, respectively, for clear as well as turbid solutions, were found to be within acceptable limits, that is, always less than 0.69 and 0.41, respectively, for clear solution and 0.65 and 0.47, respectively, for turbid solution. Conclusively, our method was successfully applied for the determination of glipizide in clear as well as turbid solutions, and it was found that the drug analyte in both types of solutions can be detected from the same calibration curve accurately and precisely and glipizide entrapped in the liposomes or in proliposomal matrix was not detected.

scholarly journals Determination of crinamidine in dietary supplements by GC-MS/MS

2018 ◽  
Vol 1 (1) ◽  
pp. 3-7
Author(s):  
Lien Le Thi ◽  
Anh Bach Thuy ◽  
Khanh Cao Cong ◽  
Ha Tran Nguyen ◽  
Hong Hao Le Thi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Dietary Supplements ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Coefficient Of Determination ◽  
Tandem Mass ◽  
Limit Of Quantification ◽  
Precision Limit

The determination of crinamidine in dietary supplements content by mass spectrometry (GC-MS/MS) was developed and validated. The method was performed on the DB5MS column (30m x 0,25mm; 0,25 μm), in combination with the tandem mass spectrometry. The parameters of the method were evaluated for selectivity, calibration curve, accuracy, precision, limit of detection (LOD) and limit of quantification (LOQ). The calibration curve was highly linear (the coefficient of determination 0.9970) within the concentration range of 5ppm-50ppm. The recovery at three concentrations were above 90,1%. The limit of detection (LOD) and limit of quantification (LOQ) of the methods were 3 and 10 mg/kg, respectively. All obtained results were acceptable according to the AOAC for dietary supplements. The method was applied to analyze 34 samples in Hanoi.

scholarly journals Characterization and Quantitation of Polyphenolic Compounds in Senna gardneri and S. georgica from the Northeast of Brazil

2018 ◽  
Vol 13 (11) ◽  
pp. 1934578X1801301
Author(s):  
Irvila Ricarte de O. Maia ◽  
Maria Teresa Salles Trevisan ◽  
Maria Goretti de V. Silva ◽  
Andrea Breuer ◽  
Robert W. Owen
Keyword(s):  
Dry Weight ◽  
Polyphenolic Compounds ◽  
Weight Basis ◽  
Rich Source ◽  
Methanol Extracts ◽  
Phytochemical Composition

Because there is a dearth of data on the taxon Senna, the aim of this study was to determine the phytochemical composition of various botanical parts of Senna gardneri Irwin & Barneby and Senna georgica Irwin & Barneby. A total of 24 polyphenolic compounds were identified in methanol extracts. The higher concentration (on a dry weight basis) of polyphenolic compounds, was detected in the leaves (29.3 g/kg) and roots (17.3 g/kg) of S. gardneri. By contrast, a lower concentration of polyphenolic compounds were detected in the roots of S. georgica (11.8 g/kg), followed by the bark (5.8 g/kg), leaves (0.7 g/kg) and fruits (0.3 g/kg). Flavonoids comprised the major polyphenolic compounds in leaves of S. gardneri (98 %) and S. georgica (100 %) and the bark of S. georgica (86 %). Whereas the composition was quite different in the roots, of which the major components were stilbenes and naphthapyrones at 87 % and 13 % in S. georgica roots and 64 % and 28 % in S. gardneri roots. The fruits of S. georgica contained only one anthraquinone. In conclusion the roots of S. gardneri (11.023 g/kg) and S. georgica (10.285 g/kg) are a rich source of stilbenes, which have promising cancer chemopreventive capacities.

scholarly journals Phytochemical Analysis of Different Parts of Acalypha Indica L.

2021 ◽  
pp. 69-77
Author(s):  
Mim E Tasmim ◽  
M Nasiruddin ◽  
MB Islam ◽  
RS Sultana
Keyword(s):  
Medicinal Plant ◽  
Dry Weight ◽  
Weight Basis ◽  
Phytochemical Analysis ◽  
Good Source ◽  
Screening Analysis ◽  
Mineral Components ◽  
Leaf Part ◽  
Different Parts ◽  
Acalypha Indica

Acalypha indica L. is an herbaceous medicinal plant, which is grown abundantly in Bangladesh. Bangladeshi people typically use various parts of this plant for various primary healthcare treatments. The current research was conducted to check and quantify various phytochemicals and mineral components of the assorted parts of Acalypha indica L. The screening analysis shows that studied all phytochemicals were present in the leaf part but not in stem and shoot parts and some were missing or trace amount in root. Total phenol, flavonoid, and tannins content on a dry weight basis ranged from 0.53-0.82 mg GAE/g, 0.02-1.47 mg QE/g and 0.21-0.32 mg TAE/g respectively. Among the phytochemicals the leaf contains the highest amount of flavonoid (1.47 mg QE/g), and lowest in stem (0.02 mg QE/g). Similarly, the amount of minerals like Fe, Zn, K and Pb was 0.23-0.40 mg/kg, 0.01- 0.021 mg/kg, 1.55-2.85 mg/kg and 0.149-0.204 mg/kg respectively. K was the largest volume of mineral for both leaf and stem. Lowest quantity of Zn was observed in most cases. This finding indicates that the studied plant might be good source of phytochemicals. J. Bio-Sci. 29(1): 69-77, 2021 (June)

A Bioanalytical Method for Quantification of Telmisartan in rat Plasma; Development, Validation and Application to Pharmacokinetic Study

2021 ◽  
pp. 2139-2144
Author(s):  
Khater Ahmed Saeed AL-Japairai ◽  
Bappaditya Chatterjee ◽  
Syed Mahmood ◽  
Samah Hamed Almurisi
Keyword(s):  
Pharmacokinetic Study ◽  
Limit Of Detection ◽  
Plasma Concentrations ◽  
Rat Plasma ◽  
Reversed Phase ◽  
Hplc Method ◽  
Pharmacokinetic Parameters ◽  
Sprague Dawley ◽  
Linear Calibration ◽  
Limit Of Quantification

The telmisartan was determined in a rat plasma using developed and validated a reversed-phase high performance liquid chromatographic (HPLC). The pre-treatment of the plasma sample involving liquid-liquid extraction using ethanol as the extracting solvent. The HPLC method validation has been shown a linear calibration curve over a plasma concentrations range of 0.7 to 10µg/mL with a correlation coefficient of 0.9979, the limit of detection and the limit of quantification were determined to be 0.025µg/ml and 0.07µg/ml, respectively. The precision and accuracy were in an acceptable limit. The pharmacokinetic parameters of telmisartan were adequately evaluated following a single oral dose (4mg/kg) in Sprague-Dawley rats. The results observed conclude that the developed bioanalytical HPLC method is appropriate and applicable as an analytical tool in the pharmacokinetic study of telmisartan.

scholarly journals Spectrophotometric determination of neomycin sulphate in tablets form via reaction with ninhydrin reagent

2019 ◽  
Vol 10 (2) ◽  
pp. 1392-1396 ◽  
Author(s):  
Khalaf F Alsamarrai ◽  
Menaa Abdulsalam Al-Abbasi ◽  
Eman Thiab Alsamarrai
Keyword(s):  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Calibration Curve ◽  
Limit Of Detection ◽  
Basic Medium ◽  
Optimal Conditions ◽  
Limit Of Quantification ◽  
Maximum Absorbance ◽  
Ninhydrin Reagent

A new, sensitive, simple and cheap spectrophotometric method for the determination of Neomycin Sulphate (NEO) in pharmaceutical forms has been developed. The method is based on the reaction between NEO and NIN in basic medium. The maximum absorbance was at 574 nm. The conditions affecting the reaction were optimized. Under the optimal conditions, the calibration curve was linear over the range of 0.0002-0.0011 mol/L. The limit of detection and limit of quantification were 5.423×10-6 mol/L, and 1.643×10-5 mol/L, RSD% of seven replicate was 0.8217- 0.8321% and Rec% was between 99.2168-100.8857%. The proposed method was successfully applied to the determination of NEO tablets form.

scholarly journals Determination of Lisinopril in Pure and Tablet form by Using 2-Hydroxynaphthaldehyde as Derivatizing Reagent

2021 ◽  
Vol 22 (1) ◽  
pp. 115-126
Author(s):  
Zahid Ali Zounr
Keyword(s):  
Limit Of Detection ◽  
Pharmaceutical Products ◽  
Molar Absorptivity ◽  
Coefficient Of Determination ◽  
Linear Calibration ◽  
Limit Of Quantification ◽  
Tablet Form ◽  
Derivatizing Reagent ◽  
Derivatization Reaction

An easy, sensitive and accurate spectrophotometric method has been developed for the determination of Lisinopril (LNP) in pure and tablet formulations based on derivatization reaction with 2-hydroxynaphthaldehyde (2HNA). The derivatization reaction was carried out in methanol solvent at pH-5.5 at 95±2C for 15 min. The linear calibration curve was obtained that obeyed the Beer’s law within the concentration range 5-50 μgmL-1 of LNP at 433 nm with a coefficient of determination R²=0.996. The recovery was in the range from 98.25-101.82 with molar absorptivity of drug 9×103 mole-1cm-1. The method was accurate and precise (intra-day variation 0.05-0.97% and inter-day 0.07-1.6%), with limit of detection (LOD) and limit of quantification (LOQ) 0.264 μgmL-1 and 0.8 μgmL-1, respectively. No interferences from the excipients were detected. The method was applied for the rapid analysis of LNP in pharmaceutical products.

scholarly journals Partly Nonparametric Approach for Determining the Limit of Detection

2004 ◽  
Vol 50 (4) ◽  
pp. 732-740 ◽  
Author(s):  
Kristian Linnet ◽  
Marina Kondratovich
Keyword(s):  
Sample Size ◽  
Calibration Curve ◽  
Degrees Of Freedom ◽  
Limit Of Detection ◽  
Clinical Chemistry ◽  
Estimation Procedure ◽  
Worked Examples ◽  
Type I ◽  
Linear Calibration ◽  
Distribution Models

Abstract Background: According to recent International Organization for Standardization (ISO) standards, the limit of detection (LoD) of an assay should be estimated taking both type I (α) and II (β) errors into account. The suggested procedure, however, supposes gaussian distributions of both blank and sample measurements and a linear calibration curve. In clinical chemistry, asymmetric, nongaussian blank distributions are common, and the calibration curve may be nonlinear. We present a partly nonparametric procedure that takes these aspects into account. Methods: Using theoretical distribution models and simulation studies, we developed a LoD estimation procedure suitable for the field of clinical chemistry that is partly based on nonparametric statistics. Results: For sample size n, the nonparametrically determined 95th percentile of the blank measurements {obtained as the value of the [n(95/100) + 0.5]th ordered observation} defines the limit for results significantly exceeding zero [limit of blank (LoB)]. The LoD is the lowest value that is likely to yield a result exceeding the LoB. LoD is estimated as: LoB + cβ × SDS, where SDS is the analytical SD of a sample with a low concentration; cβ = z1 − β/[1 − 1/(4 × f)]; z1 − β is the standard normal deviate; and f is the number of degrees of freedom for estimation of SDS. cβ is approximately equal to 1.65 for a type II error of 5%. Approaches and needed tabular values for calculation of confidence limits are presented as well as sample size. Worked examples are given to illustrate estimation and verification of the limit of detection. Simulation results are used to document performance. Conclusion: The proposed procedure appears useful for application in the field of clinical chemistry and promotes a standardized approach for estimating LoDs of clinical chemistry assays.

Influence of GA on Uptake and Accumulation of Naptalam by Bean Plants

Weed Science ◽  
1971 ◽  
Vol 19 (2) ◽  
pp. 135-137 ◽  
Author(s):  
Robert M. Devlin ◽  
Robert W. Yaklich
Keyword(s):  
Phaseolus Vulgaris ◽  
Gibberellic Acid ◽  
Leaf Area ◽  
Dry Weight ◽  
Weight Basis ◽  
Bean Plants ◽  
Naphthylphthalamic Acid ◽  
Different Parts

Bean plants (Phaseolus vulgaris L. var. Black Valentine) pretreated with gibberellic acid (hereinafter referred to as GA) absorb and accumulate considerably more N-1-naphthylphthalamic acid (naptalam) than untreated plants. All concentrations of GA from 2.9 × 10−6 to 8.6 × 10−4M were effective; peak influence occurred at 6 × 10−4M. Plants pretreated with this concentration took up 58% more naptalam than untreated plants. When the different parts of the plant were analyzed for naptalam separately, the leaf area (on a per g dry weight basis) was influenced most by GA pretreatment. The leaf area of bean plants pretreated with 2.9 × 10−4M contained 212% more naptalam than the leaf area of untreated plants. The leaf area also included all stem and petiole tissue above and including the first true leaves.

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