scholarly journals Enzymatic Carbon Dioxide Capture

2012 ◽  
Vol 2012 ◽  
pp. 1-22 ◽  
Author(s):  
Alain C. Pierre
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Final Section ◽  
Carbon Dioxide Capture ◽  
Special Section ◽  
Atmospheric Temperature ◽  
Carbonic Anhydrases ◽  
The Past

In the past decade, the capture of anthropic carbonic dioxide and its storage or transformation have emerged as major tasks to achieve, in order to control the increasing atmospheric temperature of our planet. One possibility rests on the use of carbonic anhydrase enzymes, which have been long known to accelerate the hydration of neutral aqueous CO2 molecules to ionic bicarbonate species. In this paper, the principle underlying the use of these enzymes is summarized. Their main characteristics, including their structure and catalysis kinetics, are presented. A special section is next devoted to the main types of CO2 capture reactors under development, to possibly use these enzymes industrially. Finally, the possible application of carbonic anhydrases to directly store the captured CO2 as inert solid carbonates deserves a review presented in a final section.

scholarly journals The structure of a tetrameric α-carbonic anhydrase fromThermovibrio ammonificansreveals a core formed around intermolecular disulfides that contribute to its thermostability

2014 ◽  
Vol 70 (10) ◽  
pp. 2607-2618 ◽  
Author(s):  
Paul James ◽  
Michail N. Isupov ◽  
Christopher Sayer ◽  
Vahid Saneei ◽  
Svein Berg ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Carbon Dioxide Capture ◽  
Carbonic Anhydrases ◽  
Native Form ◽  
Industrial Carbon ◽  
E Coli ◽  
Reducing Environment ◽  
Reversible Hydration ◽  
Unique Core

Carbonic anhydrase enzymes catalyse the reversible hydration of carbon dioxide to bicarbonate. A thermophilicThermovibrio ammonificansα-carbonic anhydrase (TaCA) has been expressed inEscherichia coliand structurally and biochemically characterized. The crystal structure of TaCA has been determined in its native form and in two complexes with bound inhibitors. The tetrameric enzyme is stabilized by a unique core in the centre of the molecule formed by two intersubunit disulfides and a single lysine residue from each monomer that is involved in intersubunit ionic interactions. The structure of this core protects the intersubunit disulfides from reduction, whereas the conserved intrasubunit disulfides are not formed in the reducing environment of theE. colihost cytosol. When oxidized to mimic the environment of the periplasmic space, TaCA has increased thermostability, retaining 90% activity after incubation at 70°C for 1 h, making it a good candidate for industrial carbon-dioxide capture. The reduction of all TaCA cysteines resulted in dissociation of the tetrameric molecule into monomers with lower activity and reduced thermostability. Unlike other characterized α-carbonic anhydrases, TaCA does not display esterase activity towardsp-nitrophenyl acetate, which appears to result from the increased rigidity of its protein scaffold.

scholarly journals Oxygen isotope exchange between water and carbon dioxide in soils is controlled by pH, nitrate availability and microbial biomass through links to carbonic anhydrase activity

2020 ◽  
Author(s):  
Sam P. Jones ◽  
Aurore Kaisermann ◽  
Jerome Ogee ◽  
Steven Wohl ◽  
Alexander W. Cheesman ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Microbial Biomass ◽  
Carbonic Anhydrase ◽  
Oxygen Isotope ◽  
Isotope Exchange ◽  
Microbial Community Composition ◽  
Soil Microbial Communities ◽  
Carbonic Anhydrase Activity ◽  
Carbonic Anhydrases ◽  
Oxygen Isotope Exchange

Abstract. The oxygen isotope composition (δ18O) of atmospheric carbon dioxide (CO2) can be used to estimate gross primary production at the ecosystem-scale and above. Understanding how and why the rate of oxygen isotope exchange between soil water and CO2 (kiso) varies can help to reduce uncertainty in the retrieval of such estimates. The expression and activity of carbonic anhydrases in soils are important drivers of variations in kiso. Here we estimate kiso and measure associated soil properties in laboratory incubation experiments using 44 soils sampled from sites across western Eurasia and northeastern Australia. Observed kiso exceeded theoretical uncatalysed rates indicating the significant influence of carbonic anhydrases on the variability observed among the soils studied. We identify soil pH as the principal source of variation, with greater kiso under alkaline conditions suggesting that shifts in microbial community composition or intra-extra cellular dissolved inorganic carbon gradients induce the expression of more or higher activity forms of carbonic anhydrases. We also show for the first time in soils that the presence of nitrate under acidic conditions reduces kiso, potentially reflecting the direct or indirect inhibition of carbonic anhydrases. This effect was confirmed by a supplementary ammonium nitrate fertilisation experiment conducted on a subset of the soils. Future changes in atmospheric nitrogen deposition or land-use may thus influence carbonic anhydrase activity. Greater microbial biomass also increased kiso under a given set of chemical conditions likely highlighting the ubiquity of carbonic anhydrase expression by soil microbial communities. These data provide the most extensive analysis of spatial variations in soil kiso to date and indicate key controls required to predict variations in kiso at the scales needed to improve efforts to constrain gross primary productivity using the δ18O of atmospheric CO2.

scholarly journals Active Nanointerfaces Based on Enzyme Carbonic Anhydrase and Metal–Organic Framework for Carbon Dioxide Reduction

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 1008
Author(s):  
Qian Liu ◽  
Xinwei Bai ◽  
Huy Pham ◽  
Jianli Hu ◽  
Cerasela Zoica Dinu
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Gas Sensing ◽  
Anthropogenic Activities ◽  
Metal Organic Framework ◽  
Carbonic Anhydrases ◽  
Chromatography Analysis ◽  
Metal Organic ◽  
Greenhouse Emission ◽  
Organic Framework

Carbonic anhydrases are enzymes capable of transforming carbon dioxide into bicarbonate to maintain functionality of biological systems. Synthetic isolation and implementation of carbonic anhydrases into membrane have recently raised hopes for emerging and efficient strategies that could reduce greenhouse emission and the footprint of anthropogenic activities. However, implementation of such enzymes is currently challenged by the resulting membrane’s wetting capability, overall membrane performance for gas sensing, adsorption and transformation, and by the low solubility of carbon dioxide in water, the required medium for enzyme functionality. We developed the next generation of enzyme-based interfaces capable to efficiently adsorb and reduce carbon dioxide at room temperature. For this, we integrated carbonic anhydrase with a hydrophilic, user-synthesized metal–organic framework; we showed how the framework’s porosity and controlled morphology contribute to viable enzyme binding to create functional surfaces for the adsorption and reduction of carbon dioxide. Our analysis based on electron and atomic microscopy, infrared spectroscopy, and colorimetric assays demonstrated the functionality of such interfaces, while Brunauer–Emmett–Teller analysis and gas chromatography analysis allowed additional evaluation of the efficiency of carbon dioxide adsorption and reduction. Our study is expected to impact the design and development of active interfaces based on enzymes to be used as green approaches for carbon dioxide transformation and mitigation of global anthropogenic activities.

scholarly journals Between Secularity and Post-Secularity: Critical Appraisal of Charles Taylor's Account

2019 ◽  
Vol 79 (2) ◽  
pp. 411-423 ◽  
Author(s):  
Bojan Žalec
Keyword(s):  
Final Section ◽  
Critical Appraisal ◽  
Special Section ◽  
Global Scale ◽  
The Past ◽  
The Usa ◽  
The Subject ◽  
Secular Age ◽  
Definition Of ◽  
Narrative Account

The article deals with Charles Taylor's account of the secular age. In the first part, the main constituents of Taylor's narrative account are presented: the central concepts, distinctions, definition of the subject, the aims etc. The author pays special attention to the notions of secularity, secular age, religion, and transcendence. In the second part, Taylor's genealogy of the secular age is outlined and comparatively placed in the context of other main relative forms of genealogical account. Because our age is an age of authenticity, a special section is devoted to it. The final section presents some reproaches to Taylor and evaluates their strength and the value of Taylor's contribution. Besides, some speculative »forecasts« about secularity and post-secularity in Europe, the USA, and at the global scale are presented (by reference to Taylor's account). The author concludes that despite some (serious and cogent) reproaches and second thoughts about Taylor's account, it is doubtless one of the major achievements in the area that manifests features of a paradigmatic work. It helps us a lot to understand the condition of religion not only in the past and today, but also gives us directions and guidelines, conceptual and methodological tools, and ideas to more clearly discern the forms and condition of religion in the future.

Carbon dioxide capture usingEscherichia coliexpressing carbonic anhydrase in a foam bioreactor

2016 ◽  
Vol 37 (24) ◽  
pp. 3186-3192 ◽  
Author(s):  
Stuart K. Watson ◽  
Zhenlin Han ◽  
Wei Wen Su ◽  
Marc A. Deshusses ◽  
Eunsung Kan
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Carbon Dioxide Capture

Enzymatic carbon dioxide capture using a thermally stable carbonic anhydrase as a promoter in potassium carbonate solvents

2017 ◽  
Vol 307 ◽  
pp. 49-55 ◽  
Author(s):  
Guoping Hu ◽  
Kathryn H. Smith ◽  
Nathan J. Nicholas ◽  
Joel Yong ◽  
Sandra E. Kentish ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Potassium Carbonate ◽  
Carbon Dioxide Capture ◽  
Thermally Stable

The use of carbonic anhydrase to accelerate carbon dioxide capture processes

2014 ◽  
Vol 90 (1) ◽  
pp. 3-10 ◽  
Author(s):  
Joel K. J. Yong ◽  
Geoff W. Stevens ◽  
Frank Caruso ◽  
Sandra E. Kentish
Keyword(s):  
Carbon Dioxide ◽  
Carbonic Anhydrase ◽  
Carbon Dioxide Capture
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