scholarly journals Liquid Chromatography Study on Atenolol--Cyclodextrin Inclusion Complex

2012 ◽  
Vol 2012 ◽  
pp. 1-8 ◽  
Author(s):  
Shailesh M. Buha ◽  
Girin A. Baxi ◽  
Pranav S. Shrivastav
Keyword(s):  
Liquid Chromatography ◽  
Complex Formation ◽  
Inclusion Complex ◽  
Stationary Phases ◽  
Inclusion Complexation ◽  
Dispersion Interactions ◽  
Column Temperature ◽  
Retention Factors ◽  
Wide Range ◽  
London Dispersion

Inclusion complex formation of atenolol with -cyclodextrin (-CD) has been investigated by HPLC on different stationary phases, by varying pH and concentration of -CD added as an additive in the mobile phase over a wide range of column temperature. Stationary phases of different polarity and hydrophobicity were evaluated to find the best conditions for complex formation. The optimum conditions for inclusion complexation were achieved on YMC ODS-AQ C18 ( mm, 5 μ) analytical column. The apparent formation constant () of the complex as evaluated by liquid chromatography using retention factors () was  M−1 at 25°C. The stoichiometry of the complex was 1 : 1 as is evident from the straight line plot of 1/ versus -CD concentration. The formation of inclusion complex was essentially enthalpy (−42.12 kJ/mol) driven and the binding forces included hydrophobic, van der Waals-London dispersion interactions. The enthalpy-entropy compensation criterion was used to prove the inclusion phenomena.

scholarly journals Stationary phase type and temperature effect on HPLC separation of lactic acid enantiomers

2021 ◽  
Vol 14 (1) ◽  
pp. 60-65
Author(s):  
Laura Jánovová ◽  
Katarína Hroboňová
Keyword(s):  
Lactic Acid ◽  
High Performance ◽  
Stationary Phases ◽  
Chiral Stationary Phases ◽  
Reversed Phase ◽  
Optical Isomers ◽  
Column Temperature ◽  
Retention Factors ◽  
Chromatographic Parameters ◽  
Macrocyclic Antibiotics

Abstract Lactic acid is a biologically important organic acid existing in two enantiomeric forms which are differently metabolized in the human body. In this paper, direct chiral separation of lactic acid by high performance liquid chromatography is presented. Five chiral stationary phases based on macrocyclic antibiotics were used for enantioseparation and chromatographic parameters, such as retention factors, resolution and selectivity factors, were determined under different column temperatures ranging from 5 to 45 °C. Optical isomers of lactic acid were efficiently separated using chiral stationary phases based on teicoplanin (R S = 1.9 ) and ristocetin (R S = 1.7 ) in reversed-phase separation mode at the column temperature of 25 °C.

Selectivity of stationary phases in reversed-phase liquid chromatography based on the dispersion interactions

2001 ◽  
Vol 911 (2) ◽  
pp. 177-190 ◽  
Author(s):  
Maciej Turowski ◽  
Takashi Morimoto ◽  
Kazuhiro Kimata ◽  
Hiroshi Monde ◽  
Tohru Ikegami ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Stationary Phases ◽  
Reversed Phase ◽  
Dispersion Interactions ◽  
Reversed Phase Liquid Chromatography ◽  
Phase Liquid

Selectivity-column temperature relationship as a new strategy in predicting separation of structural analogues in HPLC by using different stationary phases

RSC Advances ◽  
2015 ◽  
Vol 5 (77) ◽  
pp. 62686-62696 ◽  
Author(s):  
Shu-ying Han ◽  
Hui-min Yu ◽  
Yu-qiong Pei ◽  
Yu-mei Chi
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Stationary Phases ◽  
Column Temperature ◽  
Structural Analogues ◽  
New Strategy ◽  
Hoff Equation ◽  
Relationship Of ◽  
As Relationship

The effect of changes in column temperature on van't Hoff equation, as well as relationship of separation and column temperature in high performance liquid chromatography (HPLC) by using different stationary phases, have been discussed and compared.

scholarly journals Chiral Alkaloid Analysis

2021 ◽  
Author(s):  
Ngoc Van Thi Nguyen ◽  
Kim Ngan Huynh Nguyen ◽  
Kien Trung Nguyen ◽  
Kyeong Ho Kim ◽  
Hassan Y. Aboul-Enein
Keyword(s):  
Liquid Chromatography ◽  
Plant Growth ◽  
Stationary Phases ◽  
Racemic Mixture ◽  
Theoretical Studies ◽  
Chiral Compounds ◽  
First Choice ◽  
Wide Range ◽  
Plant Growth Stimulants ◽  
Or Applications

Alkaloids are distributed in plant kingdom and play important role in protection, germination as well as plant growth stimulants. Most of them are chiral compounds and are clinically administered as the racemic mixture, even though its enantiomers have been known to exert different pharmacological activity. Liquid chromatography using chiral stationary phases (CSP) proved to be an essential tool with a wide range of applications, including analysis of the stereochemistry of natural compounds. This review gives an overview of chiral separation alkaloids that were used in theoretical studies and/or applications in recent years. It shows the possibilities of polysaccharide CSPs have now also been established as the first-choice of chiral phases for enantiomer separation.

scholarly journals Spectral studies on inclusion complexation between 3-hydroxyflavone and 2-Hydroxypropyl-β-cyclodextrin

2018 ◽  
Vol 1 (3) ◽  
Author(s):  
Rajamohan Rajaram 2 ◽  
Arumugam Praveena 1 ◽  
Samikannu Prabu 3
Keyword(s):  
Inclusion Complex ◽  
Anticancer Activity ◽  
Cell Line ◽  
Inclusion Complexation ◽  
Precipitation Method ◽  
Spectral Studies ◽  
Luminescence Spectra ◽  
Solid Inclusion ◽  
Solid Complex ◽  
Wide Range

3-hydroxy-2-phenylchromen-4-one (HF) is a flavonols that is formed when hypoxanthine is attached to a ribose ring (also known as a ribofuranose) via a β-N9-glycosidic bond. Cyclodextrins are able to form host-guest complexes with hydrophobic molecules given the unique nature imparted by their structure.As a result, these molecules have found a number of applications in a wide range of fields. The inclusion complex of HF with 2HP-β-CD is prepared by various synthetic method such as physical method (PM), kneading method (KM) and co-precipitation method (CP). The solid inclusion complex is characterized by UV, luminescence spectra, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM) and powder X-ray diffraction (XRD). The anticancer activity of the solid complex is performed against breast cancer cell line and it is noticed that there is no much better activity than the HF alone. Both the HF and its solid complex showed the poor anticancer activity against MDA MB 231 cell line. 

scholarly journals Evaluating the Adsorbed Water Layer on Polar Stationary Phases for Hydrophilic Interaction Chromatography (HILIC)

Separations ◽  
2019 ◽  
Vol 6 (2) ◽  
pp. 19 ◽  
Author(s):  
Yong Guo ◽  
Nidhi Bhalodia ◽  
Bassel Fattal ◽  
Ioannis Serris
Keyword(s):  
Mobile Phase ◽  
Stationary Phases ◽  
Volume Ratio ◽  
Water Layer ◽  
Adsorbed Water ◽  
Polar Compounds ◽  
Hydrophilic Interaction Chromatography ◽  
Hydrophilic Interaction ◽  
Column Temperature ◽  
Wide Range

The water-rich liquid layer immobilized on the surface of the polar stationary phases is critical to the retention of polar compounds in hydrophilic interaction chromatography (HILIC). Although the presence of the adsorbed water layer has been investigated and confirmed by multiple techniques, there is a lack of quantitative measures that can be easily determined and linked to chromatographic parameters. This study proposes a simple measure termed volume ratio (the ratio of the adsorbed water layer volume and the mobile phase volume) that can be easily determined using toluene elution volume. The volume ratio values measured using the proposed method indicate that the volume of the adsorbed water layer varies in a wide range in the stationary phases commonly used in HILIC separation. It was observed that the volume ratio increases with the acetonitrile content and ammonium acetate concentration in the mobile phase. In addition, increasing the column temperature had the effect of reducing the volume ratio and diminishing the adsorbed water layer.

Analysis of Aromatic Amines in Industrial Wastewater by Capillary Gas Chromatography-Mass Spectrometry

2000 ◽  
Vol 35 (2) ◽  
pp. 245-262 ◽  
Author(s):  
Francis I. Onuska ◽  
Ken A. Terry ◽  
R. James Maguire
Keyword(s):  
Gas Chromatography ◽  
Aromatic Amines ◽  
Methylene Chloride ◽  
Solid Phase ◽  
Stationary Phases ◽  
Gas Chromatography Mass Spectrometry ◽  
Substituted Anilines ◽  
Environmental Media ◽  
Solid Phase Extraction Cartridge ◽  
Wide Range

Abstract The analysis of aromatic amines, particularly benzidines, at trace levels in environmental media has been difficult because of the lack of suitable deactivated capillary column stationary phases for gas chromatography. This report describes the use of an improved type of column as well as a method for the analysis of anilines and benzidines in water, wastewater and sewage samples. Extraction procedures are applicable to a wide range of compounds that are effectively partitioned from an aqueous matrix into methylene chloride, or onto a solid-phase extraction cartridge. The extracted analytes are also amenable to separation on a capillary gas chromatographic column and transferable to the mass spectrometer. These contaminants are converted to their N-trifluoroacetyl derivatives. Aniline and some substituted anilines, and 3,3’-dichlorobenzidine and benzidine were determined in 24-h composite industrial water, wastewater, primary sludge and final effluent samples at concentrations from 0.03 up to 2760 µg/L.

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