scholarly journals In Vitro Effects of External Pressure Changes on the Sealing Ability under Simulated Diving Conditions

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Marcus Stoetzer ◽  
Martin Ruecker ◽  
Andreas Koch ◽  
Dirk Ziebolz ◽  
Horst Kokemüller ◽  
...  
Keyword(s):  
External Pressure ◽  
Chamber Pressure ◽  
Experimental Chamber ◽  
Penetration Test ◽  
Pulp Chamber ◽  
Dye Penetration ◽  
Gutta Percha ◽  
In Vitro Effects ◽  
Pressure Changes

Aim. To measure and validate the permeability of pressure changes in correlation to different root filling techniques. Methods. Eighty extracted single-rooted teeth were randomly assigned to one of eight groups of ten teeth. Following standardized instrumentation and irrigation, root canal fillings were performed using either cold lateral condensation, a warm carrier-based gutta-percha obturation technique, a warm carrier-based Resilon, or warm gutta-percha compaction with the downpack/backfill technique. After insertion of a pressure sensor within the pulp chamber ten teeth of each group then underwent simulated dives with pressure measurement and the other ten a dye penetration test during simulated dives to 5.0 bar. Differences were analyzed statistically (P<0.05) using one-way analysis of variance (ANOVA). Results. When the warm carrier-based gutta-percha obturation technique and vertical gutta-percha obturation techniques were used, there was significant lower intrapulpal pressure to experimental chamber pressure (P>0.05). When cold lateral condensation or carrier-based Resilon as used, pressure was sometimes almost completely equalized. Conclusions. Warm gutta-percha obturation techniques provide a largely pressure-tight seal whereas the Resilon obturation technique and cold lateral condensation appear to be unsuitable to pressure changes.

scholarly journals Evaluation of Apical Sealing Ability of Four Different Sealers using Centrifuging Dye Penetration Method: An in vitro Study

2012 ◽  
Vol 13 (6) ◽  
pp. 830-833
Author(s):  
Romel Joseph
Keyword(s):  
In Vitro Study ◽  
Blue Dye ◽  
Control Groups ◽  
Root Canals ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Sealing Ability ◽  
Ah Plus ◽  
And Control

ABSTRACT Aim The aim of this in vitro study was to evaluate the apical seal obtained with four root canal sealers AH 26, Sealapex, Endoflas FS and AH Plus, with lateral condensation. Materials and methods Sixty root canals were prepared using the step-back technique. The specimens were divided into four experimental groups of 12 teeth and two control groups of 12 teeth. The experimental groups were obturated by laterally condensed gutta-percha with one of the tested sealers and control groups were obturated without any sealer. Methylene blue dye penetration with centrifuging method was used to evaluate the apical sealing ability. The quantitative apical leakage of each specimen was measured after 2 weeks. Results The results showed no significant differences between all groups except between AH Plus and Endoflas FS (<0.05). AH Plus showed significantly less leakage than Endoflas FS. Conclusion AH Plus showed the least leakage compared to AH 26, Sealapex and Endoflas FS. How to cite this article Joseph R, Singh S. Evaluation of Apical Sealing Ability of Four Different Sealers using Centrifuging Dye Penetration Method: An in vitro Study. J Contemp Dent Pract 2012;13(6):830-833.

scholarly journals Evaluation of the apical microleakage of MTA Fillapex, AH26, and Endofill sealers

2020 ◽  
Vol 23 (3) ◽  
Author(s):  
Saeedeh Galledar ◽  
Robab Farhang ◽  
Malek Abazari ◽  
Parisa Negahdar
Keyword(s):  
Root Canal ◽  
Test Group ◽  
In Vitro Study ◽  
Negative Control ◽  
Vitro Study ◽  
Control Group ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Significant Difference

Objective: Proper apical seal plays an important role in the success of root canal treatment. The most common cause of failure of root canal therapy is known as the lack of adequate apical seal. The aim of this in vitro study was to compare the apical microleakage of MTA Fillapex, , and Endofill sealers using dye penetration method. Material and Methods: In this in vitro study, 72 single-rooted extracted human teeth were selected. The teeth were randomly divided into three experimental groups of 20 and two positive and negative control groups of 6. The canals were prepared by step-back technique and then filled with gutta-percha and one of the sealers mentioned. In the positive control group, the canals were filled with gutta-percha without sealer, and in the negative control group, the canals were prepared but not filled. The teeth were immersed in 2% methylene blue dye for 72 hours. The teeth were then cut longitudinally and the level of dye penetration was measured under a stereomicroscope. Data were analyzed by SPSS ver. 19 software, ANOVA and Bonferroni post-hoc tests. Results: The mean level of dye penetration in the Endofill test group was significantly higher than that in the  and MTA Fillapex test groups. While, the observed difference between  and MTA Fillapex groups was not statistically significant (p<0.05). Conclusion: The results of this study showed that  and MTA Fillapex sealers did not show any significant difference in apical seal properties. However, their sealing strength was significantly greater than Endofill sealer.KeywordsAH26 sealer; Endofill; MTA Fillapex; Microleakag

scholarly journals Coronal microleakage in intact and carious teeth obturated using lateral compaction of gutta-percha

2008 ◽  
Vol 55 (2) ◽  
pp. 99-106
Author(s):  
Aleksandra Djeri ◽  
S. Sukara ◽  
R. Arbutina ◽  
Z. Trnic ◽  
N. Gajic
Keyword(s):  
Endodontically Treated Teeth ◽  
Human Teeth ◽  
Root Canals ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Group I ◽  
The Difference ◽  
Micrometer Scale ◽  
Carious Teeth

Introduction: Coronal microleakage is one of the most important factors for the success and longevity of endodontically treated teeth. Aim: The aim of this study was to evaluate in vitro the coronal microleakage in carious and intact teeth obturated with lateral compaction of gutta-percha using the dye penetration method. Materials and Methods: Forty extracted human teeth were allocated to two groups: Group I consisted of 20 intact teeth (10 single-rooted and 10 multi-rooted) and Group II comprised 20 carious teeth (10 single-rooted and 10 multi-rooted). Root canal preparation was performed using the Step-back technique and copious irrigation with 2.5% NaOCl. All root canals were obturated using lateral compaction and AH Plus endodontic sealer. The teeth were coronally filled with either CAVIT only or the combination of GIC and CAVIT, coated with 2 layers of nail varnish and immersed in 50% AgNO3. Afterwards, the teeth were sectioned mesio-distally using a diamond disc and linear dye penetration was evaluated using a stereo loupe with a micrometer scale at x6 magnification. Results: The results showed dye penetration in 70% of teeth filled with CAVIT and in 10% of teeth filled with GIC+CAVIT. The difference was statistically significant (p<0.05). Conclusion: Based on the obtained results, it may be concluded that the least coronal microleakage was observed in intact teeth temporarily filled with the GIC+CAVIT combination.

scholarly journals Comparative study of coronal sealing ability using root cross sections method

2007 ◽  
Vol 54 (4) ◽  
pp. 224-230
Author(s):  
Dejan Markovic ◽  
Vesna Zivojinovic ◽  
Slavoljub Zivkovic
Keyword(s):  
Cross Sections ◽  
Root Canal ◽  
Smear Layer ◽  
Blue Dye ◽  
Human Teeth ◽  
Root Canals ◽  
Dye Penetration ◽  
Gutta Percha ◽  
Significant Difference

Objective: An in vitro comparison of coronal microleakage of three root canal sealers was performed using the dye leakage method. Methodology: Ninety-eight single-rooted human teeth were instrumented and randomly allocated to two groups. In the first group, root canals were treated with EDTA to remove the smear layer. The roots in both groups were obturated using laterally condensed gutta-percha and Sealapex, AH-26 or Ketac-Endo as the sealer. The teeth were suspended in 1% methylene blue dye for 6 days. The teeth were then rinsed, sectioned perpendicular to the long axis and evaluated for coronal dye penetration. Results: In general, Ketac-Endo root canal sealer showed greater dye penetration than Sealapex and AH-26. A statistically significant difference in the coronal penetration of the dye was found in the group with intact smear layer. Conclusion: Highly significant differences between AH-26 and Ketac-Endo (p < 0.001) and Sealapex and Ketac Endo (p < 0.001) were found.

Patency of the follicular epithelium in Rhodnius prolixus: a re-examination of the hormone response and technique refinement

1980 ◽  
Vol 58 (9) ◽  
pp. 1617-1625 ◽  
Author(s):  
Erwin Huebner ◽  
Hampik S. Injeyan
Keyword(s):  
Regression Analysis ◽  
Juvenile Hormone ◽  
Evans Blue ◽  
Rhodnius Prolixus ◽  
Follicular Epithelium ◽  
Hormone Response ◽  
Penetration Test ◽  
Improved Method ◽  
Dye Penetration

The size of the extracellular spaces revealed by Evans' blue penetration into the follicular epithelium of Rhodnius prolixus increases with the stage of vitellogenesis. This was found to be true of follicles incubated in control media, as well as those whose spaces had been stimulated to expand by incubation in juvenile hormone (JH) or the analogue ZR515. An improved method of estimating hormonal effects in vitro utilizes a regression analysis of patency index relative to follicle length; with control follicles the slope was 0.44, whereas hormone- and analogue-treated follicles yielded slopes of 0.86 and 1.09, respectively. Some pitfalls of the dye penetration test are outlined.

scholarly journals Comparative evaluation of sealing ability of different obturation materials

2010 ◽  
Vol 138 (5-6) ◽  
pp. 287-291 ◽  
Author(s):  
Irena Melih ◽  
Ankica Jakovljevic ◽  
Milica Popovic ◽  
Dragana Pesic
Keyword(s):  
Root Canal ◽  
In Vitro Study ◽  
Control Group ◽  
Root Canal Filling ◽  
Single Root ◽  
Dye Penetration ◽  
Filling Materials ◽  
Gutta Percha ◽  
Ah Plus

Introduction Root canal filling materials are intended to prevent microleakage and passing of microorganisms and their toxins along the root canal space and into the periradicular tissues. Objective Objective of this in vitro study was to evaluate and compare apical leakage of root canals obturated using a modified warm vertical condensation technique and different materials. Methods Sixty-five extracted single-root teeth were prepared according to the crown-down/ step-back technique. Each canal was rinsed with 3% NaOCl during and after the preparation. The teeth were divided into 3 groups. Every group was obturated by the same technique, but with different materials: RealSeal system (SybronEndo), gutta-percha with AH Plus (Dentsply Maillefer) and gutta-percha with Acroseal sealer (Septodont). The remaining 20 teeth were in the control group. The teeth were immersed in 1% methylene blue for 72 hours. After that period, the roots were split longitudinally, and dye penetration was measured using a stereomicroscope. Results Dye penetration occurred in all groups. The least dye penetration occurred in the RealSeal group (0.33?}0.29 mm), while the highest dye penetration occurred in the Acroseal group (1.11?}0.52 mm). According to the Student's t-test, Acroseal showed significantly more leakage (p<0.001) than RealSeal and AH Plus materials. The microleakage of RealSeal system was not significantly different (p>0.05) from that of AH Plus sealer with gutta-percha. Conclusion The results suggested that new material Resilon (RealSeal) and conventional combination AH Plus with gutta-percha had the least apical dye penetration and provided the best sealing.

scholarly journals Microleakage of contemporary composite systems in dentin cavities

2009 ◽  
Vol 62 (7-8) ◽  
pp. 295-303 ◽  
Author(s):  
Igor Stojanac ◽  
Milan Drobac ◽  
Ljubomir Petrovic ◽  
Ivana Stojsin
Keyword(s):  
Dwell Time ◽  
Single Bond ◽  
Adhesive Systems ◽  
Pulp Chamber ◽  
Dye Penetration ◽  
Composite Systems ◽  
Sealing Ability ◽  
Significant Difference ◽  
Self Etch

This study evaluated microleakage in vitro of self-etch and multi-step, total-etch adhesive systems. Ninety extracted non-carious human molars were randomly assigned to nine groups (n=10) and restored with nine different composite systems: Gluma CPS/Charisma, Syntac Sprint/Tetric Ceram, 3M Single Bond/Silux, Admira Bond/Admira, Optibond Solo Plus/Herculite, Trendy Bond/Saremco, Excite/Tetric Ceram, Syntac Sprint/Compoglass and Promt-L-Pop/Silux. Cavities (2 mm diameter/2 mm deep) were prepared on the facial and lingual surfaces of each tooth previously abraded with a silicon-carbide abrasion discs to produce two level dentin surfaces with whole margins in dentin.Each group of composite systems was treated following the manufacturer's instructions. After they were finished and polished, the specimens were subjected to 100 thermal cycles in baths of 4 degrees+/-2 degrees C and 58 degrees+/-2 degrees C with 60 seconds of dwell time for each bath. After the thermocycling, the specimens were sealed with acid-resistant varnish, leaving a 1-mm window around the cervical margin interface, and immersed in 1% methylene blue buffered solution for 4 hours. Then the specimens were sectioned longitudinally in half and evaluated under stereomicroscopy at 30 times magnification, using inserted millimeter measurer. Each specimen was measured for dye penetration, thickness of composite filling and distance from the bottom of cavity to the pulp chamber. The data were analyzed using Kruskal-Wallis Analysis of Variance (ANOVA) and Pearson t- test. Syntac Sprint/Tetric Ceram, 3M Single Bond/Silux, Admira Bond/Admira, Optibond Solo Plus/Herculite and Gluma CPS/Charisma showed the least microleakage at the occlusal part of the cavity. There was no significant difference of microleakage at gingival part of cavity for all tested composite systems and the best sealing ability showed 3M Single Bond/Silux. Self-etch and multi-step, total-etch adhesive systems showed more or less similar microleakage 'in vitro' conditions and marginal sealing ability of all tested composite systems was significantly lower at gingival margins of cavities.

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