scholarly journals Progressive Extracellular Matrix Disorganization in Chemically Induced Murine Oral Squamous Cell Carcinoma

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
B. Fuentes ◽  
J. Duaso ◽  
D. Droguett ◽  
C. Castillo ◽  
W. Donoso ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Connective Tissue ◽  
Cell Carcinoma ◽  
Basal Lamina ◽  
Squamous Cell ◽  
Collagen I ◽  
Severe Dysplasia ◽  
Tumor Pathogenesis

Introduction. Oral squamous cell carcinoma (OSCC) is one of the ten most common cancers affecting the human population. Tumor pathogenesis implies a multistep process in which cells acquire features that enable them to become tumorigenic and ultimately malignant. The process of OSCC carcinogenesis can be reproduced in animal models, the OSCC induction with 4-nitroquinoline-1-oxide (4NQO) in mice is a widely used tool for studying tumor pathogenesis. Objective. The aim of the present study was to determine the progressive changes in basal lamina and connective tissue remodeling during 4NQO-induced OSCC carcinogenesis. Material and Methods. Samples were classified according to “International Histological Classification of tumors” in mild, moderate, and severe dysplasia and invasive carcinoma. Five samples of each pathologic entity and control healthy tongues were used. Immunohistochemical analysis of collagen IV as well as histochemical analysis of glycosylated molecules (PAS) and collagen I (Picro Sirius red) were performed. Results. During experimental-induced carcinogenesis by 4NQO a progressive basal lamina destruction and collagen I disorganization in adjacent connective tissue can be observed. Conclusion. Our results confirm previous studies that show alterations in extracellular matrix (ECM) in malignant lesions, validating the experimental carcinogenesis induced by 4NQO.

scholarly journals Expression of Extracellular Matrix—Laminin in Oral Squamous Cell Carcinoma: An Immunohistochemical Study

2012 ◽  
Vol 13 (2) ◽  
pp. 194-200
Author(s):  
Sonam C Kapse ◽  
Ajit V Koshy ◽  
Nirmala N Rao ◽  
Sushant S Kamat ◽  
Kamal Kiswani ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Immunohistochemical Study ◽  
Tumor Development ◽  
Treatment Strategies ◽  
Prognostic Indicators ◽  
Chi Square

ABSTRACT Aim To evaluate the expression of laminin in various grades of oral squamous cell carcinoma (OSCC) in order to determine whether this protein can be used as a marker for early detection and elucidation of oral cancer. Materials and methods Immunohistochemical staining for laminin was done on 60 selected archival blocks of histopathologically diagnosed cases of primary OSCC and the laminin expression was compared between the different histopathological grades of primary OSCC. The statistical analysis was performed by using Chi-square (÷ square) test and Gaussiantest with a probability of p < 0.05 was considered as significant. Results It was observed that laminin expression decreased with tumor progression which may be correlated to the tumor aggressiveness. Conclusion There was a gradual decrease of laminin staining with decreasing cellular differentiation, with differentiated lesions showing a more conspicuous staining of basement membrane glycoprotein than less differentiated lesions. Clinical significance An understanding of how the extracellular matrix influences tumor development and invasion is fundamental in the development of new prognostic indicators and treatment strategies for oral squamous cell carcinoma. How to cite this article Koshy AV, Rao NN, Kamat SS, Kiswani K, Kapse SC, Shaikh NA. Expression of Extracellular Matrix— Laminin in Oral Squamous Cell Carcinoma: An Immunohistochemical Study. J Contemp Dent Pract 2012;13(2):194-200.

Parenchymal-stromal switching for extracellular matrix production on invasion of oral squamous cell carcinoma

2012 ◽  
Vol 43 (11) ◽  
pp. 1973-1981 ◽  
Author(s):  
Hamdy Metwaly ◽  
Satoshi Maruyama ◽  
Manabu Yamazaki ◽  
Masayuki Tsuneki ◽  
Tatsuya Abé ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Extracellular Matrix Production ◽  
Matrix Production

scholarly journals Quantitative Assessment of Myofibroblast in Severe Dysplasia, Microinvasion and Oral Squamous Cell Carcinoma: An Immunohistochemical Study

2013 ◽  
Vol 14 (1) ◽  
pp. 34-38 ◽  
Author(s):  
Rajendra Baad ◽  
Sushma Bommanavar ◽  
Sonam C Kapse ◽  
Nanita Rathod ◽  
Jyoti Mandlik ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Quantitative Assessment ◽  
Immunohistochemical Study ◽  
Severe Dysplasia ◽  
Cancer Associated Fibroblast ◽  
Stromal Tissue ◽  
Typical Component

ABSTRACT Myofibroblast are essential for the integrity of human body by virtue of its role in wound healing and pathological organ remodeling. Myofibroblast is a universal cellular component in mammalian lesions, but not a typical component of normal untraumatized tissues. Therefore its presence in abundance in case of cancer is a matter of concern. Tumor microenvironment plays a pivotal role in tumor progression. These so called cancer associated fibroblast or myofibroblast are the major components and occur in stromal tissue during carcinogenesis processes. This study is a quantitative assessment of presence and distribution of myofibroblast in severe dysplasia, microinvasion and oral squamous cell carcinoma (OSCC). How to cite this article Kapse SC, Rathod N, Baad R, Mandlik J, Sharma AS, Bommanavar S. Quantitative Assessment of Myofibroblast in Severe Dysplasia, Microinvasion and Oral Squamous Cell Carcinoma: An Immunohistochemical Study. J Contemp Dent Pract 2013;14(1):34-38.

scholarly journals Correlation of p53 Immunoexpression with Depth of Tumor in Microinvasive Oral Squamous Cell Carcinoma

2021 ◽  
pp. 98-103
Author(s):  
Archana Sonone ◽  
Swati Patil ◽  
Alka Hande ◽  
Madhuri Gawande
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Connective Tissue ◽  
Basement Membrane ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Treatment Protocol ◽  
Depth Of Invasion ◽  
Histological Grading ◽  
Neoplastic Cells

Introduction: “Oral squamous cell carcinoma (OSCC)” is a major health issue in India, the incidence of OSCC is 3-7 times more in developing countries than developed countries. OSCC is the ‘3rd most common cancer’ in India followed by “cervical and breast cancer”. One side  of OSCC that  has not much explore is the ‘microinvasive squamous cell carcinoma’ which is an early stage neoplasm  without infiltration in the deeper tissues. There is no particular definition of “microinvasive oral squamous cell carcinoma (MIOSCC)” There are no specific guideline are present to categories the “microinvasive squamous cell carcinoma (MIOSCC)”. Most of the time the infiltrating neoplastic cells are masked under the background of the inflammatory cell infiltrate present connective tissue stroma. So this study is humble attempt to recognized and measured depth of invasion of infiltrative neoplastic cells to categories MIOSCC and to find better management protocol for it Aim: This study aims to: Measure p53 immunoexpression  in “microinvasive oral squamous cell carcinoma, evaluate the depth of invasion in MIOSCC  in H & E stained section, and correlate the  p53 immunoexpression with  the depth of tumor in it. Methodology: The  25 cases of  “microinvasive oral squamous cell carcinoma” will be selected  and 10 cases of “normal oral mucosa (NOM)” will be   obtained from “gingiva and vestibular mucosa” as controls  after extraction of impacted teeth. “The depth of tumor”  will  be measured from the “basement membrane or in areas of basement membrane loss, from an imaginary line reconstructing the basement membrane from the adjacent epithelium to the deepest point of invasion in connective tissue” by  Leica DMLB2 research microscope with Leica Q-win standard software (Switzerland). Results: The results  show that   the depth of invasion in MIOSCC, will be  categorized the lesion and give the better guidelines for histological grading and treatment protocol for MIOSCC Conclusion: There are no definite guidelines for histological grading and final treatment protocol for MIOSCC. The assessments of depth of tumor through p53 immunoexpression may be one of the criteria for grading in MIOSCC. Thus the correlation of p53 immunoexpression with the depth of tumor in MIOSCC helps to determine the treatment modalities of MIOSCC.

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