scholarly journals Development and Evaluation of Environmentally Benign Aqueous Two Phase Systems for the Recovery of Proteins from Tannery Waste Water

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Selvaraj Raja ◽  
Vytla Ramachandra Murty
Keyword(s):  
Waste Water ◽  
Sodium Citrate ◽  
Potassium Citrate ◽  
Ammonium Citrate ◽  
Bottom Phase ◽  
Two Phase ◽  
Tannery Waste ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Tannery Waste Water

Aqueous two phase systems (ATPSs) containing high molecular weight polyethylene glycol, PEG (10000), and completely biodegradable citrate salts (sodium citrate, potassium citrate, and ammonium citrate) with water were developed to recover the waste water proteins from a model tannery waste water system. The variations in the phase diagram were explained on the basis of ionic radius of cations, Gibbs free energy of hydration (ΔGhydation) of cations, and effective excluded volume (EEV) of salts. The salting-out capability of the cations followed the sequence: Sodium citrate > Potassium citrate > Ammonium citrate. Setschenow-type equations were used to correlate tie-line compositions. During partitioning studies, recovery of tannery waste water proteins in PEG 10000 + Sodium citrate ATPS was found superior to other systems. It was possible to recover 95.86% of proteins from tannery waste water in the bottom phase with PEG 10000 30% (w/w) + Sodium citrate 13% (w/w) at 30°C. The partition coefficients were correlated with the salt compositions by a quadratic equation and the coefficients were calculated.

scholarly journals Phase Separation Behavior and System Properties of Aqueous Two-Phase Systems with Polyethylene Glycol and Different Salts: Experiment and Correlation

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Haihua Yuan ◽  
Yang Liu ◽  
Wanqian Wei ◽  
Yongjie Zhao
Keyword(s):  
Phase Separation ◽  
Sodium Citrate ◽  
Line Length ◽  
Continuous Phase ◽  
Two Phase ◽  
Separation Rate ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
System Properties ◽  
Tie Line

The phase separation behaviors of PEG1000/sodium citrate, PEG4000/sodium citrate, PEG1000/ammonium sulfate, and PEG4000/ammonium sulfate aqueous two-phase systems were investigated, respectively. There are two distinct situations for the phase separation rate in the investigated aqueous two-phase systems: one state is top-continuous phase with slow phase separation rate and strong bottom-continuous phase with fast phase separation rate and weak volume ratio dependence. The system properties such as density, viscosity, and interfacial tension between top and bottom phases which have effects on the phase separation rate of aqueous two-phase systems were measured. The property parameter differences between the two phases increased with increasing tie line length and then improved the phase separation rate. Moreover, a modified correlation equation including the phase separation rate, tie line length, and physical properties of the four aqueous two-phase systems has been proposed and successfully tested in the bottom-continuous phase, whose coefficients were estimated through regression analysis. The predicted results of PEG1000/sodium citrate aqueous two-phase systems were verified through the stationary phase retention in the cross-axis countercurrent chromatography.

Liquid-Liquid Equilibria for Acetone + Several Citrates Aqueous Two-Phase Systems at Different Temperatures

2012 ◽  
Vol 549 ◽  
pp. 30-35
Author(s):  
Shi Ping Hu ◽  
Juan Han ◽  
Yong Sheng Yan ◽  
Yu Tao Hu
Keyword(s):  
Ternary Systems ◽  
Data Fitting ◽  
Potassium Citrate ◽  
Liquid Equilibrium ◽  
Two Phase ◽  
Different Temperatures ◽  
Tie Lines ◽  
Aqueous Two Phase Systems ◽  
Phase Systems ◽  
Tie Line

Liquid-liquid equilibria for the three kinds of the ternary systems acetone + ammonium, sodium or potassium citrate + water have been determined at T= (273.15, 283.15, and 298.15) K. Binodal curves, tie-lines, and integrated phase diagrams for the ternary systems are given. The data of the experimental bimodal curve are described with a four-parameter equation. The result also shows the temperature has little influence on the liquid-liquid equilibrium within the investigated range. The tie-line data calculated according to the bimodal data fitting equation and the lever arm rule were satisfactorily described by using the Othmer-Tobias and Bancroft equations, and the result conform the reliability of the calculation method and corresponding tie-line data.

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