scholarly journals Improved Mannanase Production from Penicillium occitanis by Fed-Batch Fermentation Using Acacia Seeds

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Monia Blibech ◽  
Raoudha Ellouz Ghorbel ◽  
Fatma Chaari ◽  
Ilyes Dammak ◽  
Fatma Bhiri ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Shake Flask ◽  
Fold Increase ◽  
Batch Cultivation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Culture Process ◽  
Coconut Meal ◽  
Penicillium Occitanis ◽  
Shake Flask Fermentation

By applying a fed-batch strategy, production of Penicillium occitanis mannanases could be almost doubled as compared to a batch cultivation on acacia seeds (76 versus 41 U/mL). Also, a 10-fold increase of enzyme activities was observed from shake flask fermentation to the fed-batch fermentation. These production levels were 3-fold higher than those obtained on coconut meal. The high mannanase production using acacia seeds powder as inducer substrate showed the suitability of this culture process for industrial-scale development.

Optimization of fermentation conditions for squalene production by heterotrophic marine microalgae Schizochytrium mangrovei PQ6

2017 ◽  
Vol 39 (3) ◽  
pp. 349-358
Author(s):  
Nguyen Cam Ha ◽  
Hoang Thi Minh Hien ◽  
Le Thi Thom ◽  
Hoang Thi Huong Quynh ◽  
Dang Diem Hong
Keyword(s):  
Batch Fermentation ◽  
Monosodium Glutamate ◽  
Vitamin B1 ◽  
Batch Cultivation ◽  
Marine Microalgae ◽  
Physical Factors ◽  
Fed Batch ◽  
Fermentation Conditions ◽  
Fed Batch Fermentation ◽  
Optimization Of Fermentation

Squalene is currently known as chemopreventive agents in reducing the incident of coronary heart disease and cancer. It is also a strong antioxidant used extensively in the food and cosmetic industries. Microbial sources of squalene are being explored in recent years. The objective of this study was to investigate fermentation conditions of Schizochytrium mangrovei PQ6 for high squalene production in 30L bioreactor. Squalene production was influenced by various nutritional factors such as glucose, nitrogen, vitamins etc. and physical factors such as batch and fed-batch fermentation. Our study established that fed-batch fermentation with nitrogen source of 1.2% yeast extract and 6.4% monosodium glutamate and 0.4% mixture of vitamin B1, 6 and 12 as optimal culture condition for maximal production of squalene by S. mangrovei PQ6. In fed-batch culture, the glucose concentration feeding was added up to 22% into the culture at 36 h of fermentation. The maximum squalene production was achieved 6.9 ± 0.1 g/L at 48 h of fed-batch fermentation, respectively. The values were 2-3 times more than that in fed-batch cultivation without adding mixture of vitamins and 4-6 times more than that in batch cultivation. Our obtained results are a breakthrough for the development of alternative squalene source from heterotrophic marine microalgae of Schizochytrium mangrovei PQ6 species for health-promoting functional foods.   Citation: Nguyen Cam Ha, Hoang Thi Minh Hien, Le Thi Thom, Hoang Thi Huong Quynh, Dang Diem Hong, 2017. Optimization of fermentation conditions for squalene production by heterotrophic marine microalgae Schizochytrium mangrovei PQ6. Tap chi Sinh hoc, 39(3): 349-358. DOI: 10.15625/0866-7160/v39n3.9130. *Corresponding author: [email protected]; [email protected] Received 6 January 2017, accepted 20 August 2017

scholarly journals Bioethanol Production From Pennisetum sp. Using Fed-Batch Simultaneous Saccharification and Co-Fermentation at High Solid Loadings and Its Life Cycle Analysis

2020 ◽  
Author(s):  
Sonai Mohapatra ◽  
Bikash Chandra Behera ◽  
Achuyta Nanda Acharya ◽  
Hrudayanath thatoi
Keyword(s):  
Life Cycle ◽  
Ethanol Production ◽  
Life Cycle Analysis ◽  
Batch Fermentation ◽  
Shake Flask ◽  
Feeding Strategy ◽  
Bioethanol Production ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Simultaneous Saccharification

Abstract Considerable progress has been achieved for production of bioethanol from lignocellulosic biomass. However, increasing the substrate concentration has shown to decrease the ethanol productivity. In the present study, Saccharomyces cerevisiae and Pichia membreneferans were used for ethanol production from glucose and xylose sugars respectively in optimized conditions. Further, co-culture fermentations were conducted in three different strategies for 13 g of sugar (10 g of glucose and 3 g of xylose) and the best strategy was further used for ethanol production from ultrasonication assisted NaOH (UA-NaOH) pretreated and enzymatically saccharified in batch and fed-batch fermentation conditions. Further, fed-batch fermentation was used for separate hydrolysis and co-fermentation (SHCF) and simultaneous saccharification and co-fermentation (SSCF) in shake flask conditions. The highest ethanol production of 12.2 and 7.9 (g/L) was observed for fed-batch SSCF denannath grass (DG) and Hybrid napier grass (HNG) (Palkonal MBW as the enzyme) biomass (80 g) respectively in shake flask conditions. However, increasing the biomass concentration to 270 g produced an ethanol concentration of 77.6 and 51.3 (g/L) for DG and HNG respectively in fed-batch SSCF conditions in bioreactor. Nuclear magnetic resonance studies of the residual biomass of both DG and HNG revealed presence of lower carbohydrate content, demonstrating the efficiency of the fermentation strategy. Further Life cycle Analysis (LCA) was also conducted to analyzed the effect of the ethanol on different environmental conditionsThe substrate feeding strategy and the saccharifying enzymes play a major role for efficient bioethanol production with higher substrate loadings. Presence of lower carbohydrates and some lignin moieties demonstrating the efficiency of the SSCF strategy for maximum conversion of carbohydrates. Thus fed batch SSCF process can be considered as a promising technique for biorefinery based bioethanol production from Pennisetum sp. in the future.

Glycerol Synthesis Attenuation ofCandida glycerinogenesin Fed-batch Fermentation

2012 ◽  
Vol 18 (5) ◽  
pp. 791
Author(s):  
Xiaoyun DING ◽  
Bin ZHUGE ◽  
Huiying FANG ◽  
Hong ZONG ◽  
Xiaoxiao LIU ◽  
...  
Keyword(s):  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation ◽  
Glycerol Synthesis

Run-to-Run Optimization of Fed-Batch Fermentation for Ethanol Production

2010 ◽  
Vol 33 (9) ◽  
pp. 1488-1494 ◽  
Author(s):  
W.-H. Huang ◽  
G. S. Shieh ◽  
F.-S. Wang
Keyword(s):  
Ethanol Production ◽  
Batch Fermentation ◽  
Fed Batch ◽  
Fed Batch Fermentation
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