scholarly journals Prepubertal Daidzein Exposure Enhances Mammary Gland Differentiation and Regulates the Expression of Estrogen Receptor-Alpha and Apoptotic Proteins

ISRN Oncology ◽  
2011 ◽  
Vol 2011 ◽  
pp. 1-9 ◽  
Author(s):  
Prachi Mishra ◽  
Anand Kar ◽  
R. K. Kale
Keyword(s):  
Estrogen Receptor ◽  
Mammary Gland ◽  
Dna Fragmentation ◽  
Estrogen Receptor Alpha ◽  
Caspase 3 ◽  
Estradiol Benzoate ◽  
Dimethyl Sulphoxide ◽  
Terminal End Buds ◽  
Mammary Gland Differentiation ◽  
Apoptosis Level

Mechanism of chemoprevention by daidzein (500 μg/g bwt) was examined by injecting it subcutaneously at 16th, 18th, and 20th day postpartum, followed by counting of terminal end buds (TEBs), terminal ducts (TDs), and lobules and immunohistochemistry of ER-α, Bcl2, Bax, and caspase-3. DNA fragmentation was also analysed to measure the apoptosis level. Estradiol benzoate (EB) (500 ng/g bwt) and dimethyl sulphoxide (DMSO) were used as reference and vehicle, respectively. Observations show a significant enhancement of mammary gland differentiation at postnatal day 21 (PND21) as well as PND50. There was a significant decrease of ER-α expression at PND21 and increase in its expression at PND50, in daidzein-treated animals. The ratio of expression of Bcl-2 to Bax proteins increased at PND50 the same whereas, it decreased at PND50 due to daidzein. An increased expression of caspase-3 and DNA fragmentation was also seen due to daidzein at PND50. The mammary gland of EB-treated animals showed response a somewhat similar to that of daidzein-treated animals.

scholarly journals Spontaneous Basaloid Adenomas of the Mammary Gland in Four Dogs: Clinicopathologic and Immunohistochemical Features

2002 ◽  
Vol 39 (6) ◽  
pp. 739-743 ◽  
Author(s):  
J. Martín de las Mulas ◽  
J. Ordás ◽  
M. Y. Millán ◽  
A. Espinosa de los Monteros ◽  
C. Reymundo
Keyword(s):  
Estrogen Receptor ◽  
Mammary Gland ◽  
Epithelial Cells ◽  
Tumor Cells ◽  
Basal Cell ◽  
Estrogen Receptor Alpha ◽  
Progesterone Receptors ◽  
Receptor Alpha ◽  
Contraceptive Steroids

Spontaneous basaloid adenomas occurred in four out of 354 dogs with mammary tumors. Affected dogs were pure-bred, intact females between 6 and 8 years of age. Three dogs were nuliparous, two had pseudopregnancies, and none had received contraceptive steroids. The tumors were multiple (three cases) or unique, less than 1 cm in diameter, well delineated, and composed of uniform cords and clusters of monomorphic epithelial cells with focal signs of squamous or glandular differentiation. A basal cell immunophenotype (cytokeratins 5 and 14 positive) without either glandular epithelial (cytokeratins 8, 18, and 19 negative) or myoepithelial (calponin negative) differentiation was observed in the majority of tumor cells. No recurrence or metastasis was recorded after follow-up periods between 3 and 24 months. In spite of the hormone-dependent nature of this tumor in female Beagles given experimental contraceptive steroids, spontaneous basaloid adenomas lacked estrogen receptor alpha and progesterone receptors.

Estrogen receptor alpha (ERα) and progesterone receptor (PR) in the mammary gland of bitches during different stages of estrous cycle

2009 ◽  
Vol 19 (2) ◽  
pp. 173-178
Author(s):  
Sukanya Manee-in ◽  
Sayamon Srisuwatanasagul ◽  
Chainarong Lohachit ◽  
Junpen Suwimonteerabutr ◽  
Sudson Sirivaidyapong
Keyword(s):  
Estrogen Receptor ◽  
Mammary Gland ◽  
Progesterone Receptor ◽  
Estrous Cycle ◽  
Estrogen Receptor Alpha ◽  
Receptor Alpha
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