scholarly journals ISOLAMENTO DE MICRORGANISMOS DEGRADADORES DE COMPOSTOS LIPÍDICOS DE ORIGEM VEGETAL EM AMOSTRAS DE ÁGUAS DA BARRAGEM DO RIO PASSAÚNA-ARAUCÁRIA, PR

2003 ◽  
Vol 21 (1) ◽  
Author(s):  
JUÇARA FEITOSA ◽  
IDA CHAPAVAL PIMENTEL ◽  
MÁRCIA REGINA BEUX ◽  
CARLOS ROBERTO DALKE ◽  
FABIANA ZARA PASTRO ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Culture Media ◽  
Soy Oil ◽  
Selection Of

Avaliou-se o potencial de degradação de compostos lipídicos, naturalmente presentes em amostras de água da Barragem do Rio Passaúna (Curitiba-Paraná/Brasil) ou artificialmente incorporados a meios de cultura, por cepas de Pseudomonas aeruginosa e por microrganismos nãoselecionados. Os resultados demonstraram degradação do substrato lipídico (óleo de soja virgem e utilizado) apenas em relação ao experimento em que não houve o isolamento e seleção prévia de cepas específicas. ISOLATION OF MICROORGANISMS THAT DEGRADE LIPIDIC COMPOUNDS OF VEGETAL ORIGIN IN WATER SAMPLES OF PASSAÚNA RIVER DAM – ARAUCÁRIA-PR (BRAZIL) Abstract It was evaluated the degradation potential of lipidic compounds naturally present in water samples of Passaúna River dam (Curitiba – Paraná/Brazil) or artificially incorporated to culture media by strains of Pseudomonas aeruginosa and by non-selected microorganisms. The results demonstrated degradation of the lipidic substrate (virgin soy oil and already used) only in relation to the experiment where no isolation and previous selection of specific strains was made.

scholarly journals Semiautomated detection of Pseudomonas aeruginosa from diverse water samples using highly selective Z-broth and a redox potential monitor

2020 ◽  
Author(s):  
Géza Szita ◽  
Szakmár Katalin ◽  
Sándor Bernáth ◽  
József Sövényi ◽  
Gábor Fülöp ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Drinking Water ◽  
Redox Potential ◽  
Water Samples ◽  
Culture Media ◽  
Broth Culture ◽  
List Type ◽  
Swimming Pools ◽  
Hospital Environments ◽  
Potential Monitoring

AbstractPseudomonas aeruginosa is a facultative bacterial pathogen with increasing public health risk potential. Contaminated water in hospital environments is a growing cause of multidrug-resistent P. aeruginosa nosocomial infections that are life-threatening and are costly to treat. P. aeruginosa is common in natural water bodies, but it is not unusual in drinking water and has been detected in bottled water, also. Suppliers in Europe must eliminate live forms of the bacterium in drinking water to meet human consumption requirements. Laboratory testing for the presence of viable P. aeruginosa is mostly done manually using culture media but conductance/impedimetry measurements for detection are also available. In order to improve expedience and cost efficiency as well as to automate the detection and the data registration, we applied the highly selective Z-broth culture media and redox potential monitoring to detect P. aeruginosa from water. The Z-broth is based on only a few, stable chemicals that provide consistency of quality and long shelf life. It limits growth to P. aeruginosa and, thus, eliminates the need for subsequent microbiological identification steps of the European Standard procedure defined in ISO 16 266:2006 (2018). A redox potential monitor was used in this work, that simultaneously recorded 64 sample curves and automatically marked positive samples reliably within 24 hours after sample initialization. In comparison, the standard method requires additional tests that prolongs identification to some days. Practical applicability and reliability of the method in this paper was demonstrated by testing a total of 739 water samples of which 222 were tap water, 342 well water and 145 had been taken from swimming pools.It is considered this method is well suited to process large numbers of samples for purposes of detecting P. aeruginosa contamination with relative ease, little cost in shortened time. Using Z-broth in combination with redox potential monitoring can be recommended for central laboratories for routine testing of drinking water for live P. aeruginosa presence. As well, using this method for testing of fluids and surfaces for P. aeruginosa contamination would be advantageous in such environments as the hospital industry, swimming pools and spas, where cleaning routines and P. aeruginosa transmission prevention protocols could be made much more resultful and cost-effective due to ease and speed of processing both spot check and water samples.HighlightsA method was applied for detection of viable Pseudomonas aeruginosa in 739 water samples.Redox potential monitoring of Z-broth cultures exclusively registered growth of P. aeruginosa.The method is highly selective, reliable and partly automated, with lower labor costs.The method is suited for testing of water and hospital environments for P. aeruginosa.

The Study of Bacillus Subtils Antimicrobial Activity on Some of the Pathological Isolates

2019 ◽  
Vol 9 (02) ◽  
Author(s):  
Hussein A Kadhum ◽  
Thualfakar H Hasan2
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Bacterial Growth ◽  
Broad Spectrum ◽  
Inhibitory Activity ◽  
Bacterial Pathogens ◽  
Inhibitory Ability ◽  
Selection Of

The study involved the selection of two isolates from Bacillus subtilis to investigate their inhibitory activity against some bacterial pathogens. B sub-bacteria were found to have a broad spectrum against test bacteria such as Staphylococcus aureus and Pseudomonas aeruginosa. They were about 23-30 mm and less against Klebsiella sp. The sensitivity of some antibodies was tested on the test samples. The results showed that the inhibitory ability of bacterial growth in the test samples using B. subtilis extract was more effective than the antibiotics used.

Identification Pseudomonas aeruginosa by OprD Gene for Differentiation from Other Pseudomonas Species that Isolated from Clinical Samples

2019 ◽  
Vol 9 (01) ◽  
pp. 46-50
Author(s):  
Ashwak B Al-Hashimy ◽  
Huda S Alagely ◽  
Akeel K Albuaji ◽  
Khalid R Majeed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
General Hospital ◽  
Culture Media ◽  
Final Diagnosis ◽  
Clinical Samples ◽  
Bacterial Isolates ◽  
Molecular Method ◽  
Biochemical Tests ◽  
Pseudomonas Species ◽  
Specific Sequences

The present study included the collection of 100 samples from various clinical sources for investigating the presence of P. aeruginosa in those sources, the samples have been collected from some hospitals in Baghdad and Hillah city (Al-qassim General Hospital, ,Al-hillah teaching hospital,and Al-hashimya General hospital ) which included wounds, burns, ear and sputum infections. The study was carried out through October 2017 till the end of March 2018. The samples were identified based on the morphological and microscopically characteristics of the colonies when they were culturing or number of culture media as well as biochemical tests, molecular identification were also used as a final diagnostic test for isolates that were positive as they belong to P.aeruginosa bacteria during previous tests based on the OprD gene which has specific sequences for P.aeruginosa bacteria as a detection gene and also consider as virulence factor so it have a synonyms mechanism to antibiotic resistance . The results of the final diagnosis showed that 38 isolates belong to target bacteria were distributed as 18 of burns, 11 isolates of wounds, 6 isolates of ear infection and 3 isolates of sputum, The examination of the sensitivity of all bacterial isolates was done for elected 38 isolation towards the 9 antibiotic by a Bauer - Kirby and the isolates were resistant for a number of antibiotics used such as Ciprofloxacin 65.7%, Norflaxacin 71%, Imipenem 63.1% Meropenem 68.4%, Gentamicin 65.7%, Amikacin 26.3%, Cefepime 68.4%, Ceftazidime 65.7% and Piperacillin 57.8%.Molecular method , All isolates (38) of P. aeruginosa positive for the diagnostic special gene (OprD) genes (100%).

scholarly journals Detection of Pseudomonas aeruginosa in water samples using a novel synthetic medium and impedimetric technology

2007 ◽  
Vol 45 (1) ◽  
pp. 42-46 ◽  
Author(s):  
G. Szita ◽  
M. Gyenes ◽  
L. Soós ◽  
T. Rétfalvi ◽  
L. Békési ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Water Samples ◽  
Synthetic Medium

Modern nanobiotechnologies for efficient detection and remediation of mercury

Sensor Review ◽  
2021 ◽  
Vol ahead-of-print (ahead-of-print) ◽  
Author(s):  
Mulayam Singh Gaur ◽  
Rajni Yadav ◽  
Mamta Kushwah ◽  
Anna Nikolaevna Berlina
Keyword(s):  
Heavy Metals ◽  
Water Samples ◽  
Low Cost ◽  
High Sensitivity ◽  
Environmental Water ◽  
Mercury Ions ◽  
Content Type ◽  
Efficient Detection ◽  
Sensitivity And Selectivity ◽  
Selection Of

Purpose This information will be useful in the selection of materials and technology for the detection and removal of mercury ions at a low cost and with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. The purpose of this study is to provide the useful information for selection of materials and technology to detect and remove the mercury ions from water with high sensitivity and selectivity. Design/methodology/approach Different nano- and bio-materials allowed for the development of a variety of biosensors – colorimetric, chemiluminescent, electrochemical, whole-cell and aptasensors – are described. The materials used for their development also make it possible to use them in removing heavy metals, which are toxic contaminants, from environmental water samples. Findings This review focuses on different technologies, tools and materials for mercury (heavy metals) detection and remediation to environmental samples. Originality/value This review gives up-to-date and systemic information on modern nanotechnology methods for heavy metal detection. Different recognition molecules and nanomaterials have been discussed for remediation to water samples. The present review may provide valuable information to researchers regarding novel mercury ions detection sensors and encourage them for further research/development.

Comparative study of membrane filtration and enrichment media for the isolation and enumeration of Pseudomonas aeruginosa from sewage, surface water, and swimming pools

1985 ◽  
Vol 31 (8) ◽  
pp. 686-692 ◽  
Author(s):  
A. H. Havelaar ◽  
M. During ◽  
E. H. M. Delfgou-Van Asch
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Membrane Filtration ◽  
Culture Media ◽  
Polluted Water ◽  
Most Probable Number ◽  
Cellulose Ester ◽  
Swimming Pools ◽  
Probable Number ◽  
Membrane Filters ◽  
Swimming Water

The recovery of Pseudomonas aeruginosa on several selective culture media was tested using raw sewage and secondary sewage effluent samples as well as spiked chlorinated imitation swimming water and samples from whirlpools. mPA-medium B gave good recovery of both vital and chlorine-injured P. aeruginosa and selectivity was greater than 90% when analysing whirlpool samples. It is therefore the medium recommended for examination of chlorinated swimming pools. When analysing sewage polluted water with the mPA-B medium, reduced selectivity was noted from low verification rates and from overgrowth by competitive flora. A modified medium (mPA-D; addition of cetrimide, omission of sulphapyridine and actidione) was more selective and sufficiently recovered noninjured cells. Chlorine-injured cells were completely inhibited, however. C-390 (9-chloro-9-(4-diethylaminophenyl)-10-phenylacridan) was confirmed to be highly selective for P. aeruginosa when used in spread plates at a concentration of 30 μg/mL; P. aeruginosa was slightly inhibited. However, the medium could not be used with conventional membrane filtration techniques, because cellulose ester filters interfered with the selective action of C-390. Selectivity could be improved by using Gelman Tuffryn (polysulphone) filters and increasing the C-390 concentration to 120 μg/mL. At this concentration, however, the medium was strongly inhibitory to P. aeruginosa; resuscitation only partially improved recovery. Two other membrane filtration media were tested. Both cetrimide – nalidixic acid agar and Drake's medium No. 19 were inhibitory to chlorine-injured cells. Several types of membrane filters were tested and there was little difference between them. In the most-probable-number technique, recovery of P. aeruginosa was shown to be excellent when using asparagine broth. Malachite green broth was strongly inhibitory to chlorine-injured P. aeruginosa.

scholarly journals Selection of filamentous fungi that are resistant to the herbicides atrazine, glyphosate and pendimethalin

2021 ◽  
Vol 43 ◽  
pp. e51656
Author(s):  
Nara Priscila Barbosa Bravim ◽  
Anatércia Ferreira Alves ◽  
José Fábio França Orlanda ◽  
Patricia Barbosa Rodrigues Silva
Keyword(s):  
Filamentous Fungi ◽  
Mycelial Growth ◽  
Culture Medium ◽  
Agricultural Soils ◽  
Culture Media ◽  
Fusarium Verticillioides ◽  
Fungal Growth ◽  
Relative Growth ◽  
Maximum Inhibition ◽  
Selection Of

The objective of the present study was to isolate fungi from agricultural soils and evaluate fungal growth in culture medium contaminated with atrazine, glyphosate and pendimethalin. Filamentous fungi were isolated from agricultural soils and cultured in a modified culture medium containing 0, 10, 20, 50, and 100 μg mL-1 atrazine, glyphosate and pendimethalin for 14 days at 28°C. The fungi that presented optimal and satisfactory growth were plated in Sabouraud culture medium with 4% dextrose and containing the herbicides at concentrations of 0, 10, 20, 50, and 100 μg mL-1 for seven days at 28°C. The mean mycelial growth values were submitted to analysis of variance and the Tukey test (p < 0.05%) for comparison and relative growth determination, and maximum inhibition rates were calculated. The isolated fungi Aspergillus fumigatus, Fusarium verticillioides and Penicillium citrinum were shown to be resistant to atrazine, glyphosate and pendimethalin. F. verticillioides showed higher mean mycelial growth in the culture media contaminated with atrazine and glyphosate than the other two fungi. In the culture medium contaminated with pendimethalin, F. verticillioides, and A. fumigatus presented the highest mean mycelial growth values.

scholarly journals Choice of sanitary-indicative microorganisms for the assessment of the safety of split-systems

2019 ◽  
Vol 95 (3) ◽  
pp. 296-301
Author(s):  
U. A. Rakhmanin ◽  
S. E. Shibanov ◽  
Sergey V. Kozulya
Keyword(s):  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Air Conditioning ◽  
Air Conditioning System ◽  
Split Systems ◽  
Air Conditioning Systems ◽  
Cleaning And Disinfection ◽  
Epidemiological Link ◽  
And Staphylococcus Aureus ◽  
Selection Of

Purpose of work is a compilation of data about the microflora which colonizes a split-system, with the aim of selection of sanitary-indicative microorganisms, whose presence in the sample would indicate to the need for cleaning and disinfection of split-systems. Materials and methods. In the article there were used data of five years author’s scientific inquiry, related to the prevention of respiratory diseases, associated with the usage of a local air conditioning systems. We also use the data from the literature. Results. For selection of “indicative” microorganisms, we proposed the usage of nine criteria, each of them have numeric value from 0 to 3 points (risk for health, prevalence rate of the disease, epidemiological link, speed of split system’s colonization, difficulty of cultivation, resistance in the environment, resistance to disinfectants, frequency of detection in home air conditioning systems, frequency of detection in air conditioning systems of public buildings). After the calculation Pseudomonas aeruginosa and Staphylococcus aureus received maximal score (20 points). Therefore, these two types of bacteria are indicative microorganisms. The detection of these microorganisms in split systems will indicate to the contamination of air-conditioning system. This microflora also is a criterion of cleaning and disinfection quality - presence of these microorganisms in the samples after this process will mean that the processing of air conditioning systems was performed poorly. Conclusions. Split systems are very faster colonized by conditionally pathogenic and pathogenic microflora. To prevent the possible hazard for population’s health it is necessary to develop the normative base, according to which sanitary-and-hygienic control over the split-systems working must be carried out. Proposed criteria suggest that Pseudomonas aeruginosa and Staphylococcus aureus are indicative microorganisms, and it’s identification in the air-conditioning system would mean risk for health and necessity for cleaning and disinfection.

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