scholarly journals AVALIAÇÃO IN VITRO DA AÇÃO ANTIFÚNGICA DO DIGLUCONATO DE CLORHEXIDINA E NISTATINA NO CONTROLE DO CRESCIMENTO DE Candida albicans

2003 ◽  
Vol 4 (2) ◽  
Author(s):  
Nayanna Coutinho De FARIAS ◽  
Marilene Magalhães BUFFON ◽  
Rafael CINI
Keyword(s):  
Candida Albicans ◽  
Chlorhexidine Digluconate ◽  
Agar Culture ◽  
In Vitro Evaluation ◽  
Antifungal Properties ◽  
Yeast Strains ◽  
Antifungal Action ◽  
Chlorhexidine Solution

O estudo tem como objetivo avaliar comparativamente as propriedades antifúngicas da nistatina 100.000 UI em relação à solução de digluconato de clorhexidina a 0.2%. A suspensão de C. albicans foi semeada em meio de cultura de BHI com ágar, com cerca de 6mm de espessura distribuídos em placas de petri com escavações de 7mm de diâmetro. Após 10 repetições, obteve-se os resultados de acordo com o halo de inibição formado pela solução. Para o digluconato de clorhexidina o halo de inibição foi de 27mm e para nistatina foi 17mm. Assim, pode-se considerar a clorhexidina 0,2% no estudo in vitro como superior no combate antifúngico das cepas de leveduras em relação à nistatina. IN VITRO EVALUATION OF ANTIFUNGAL ACTION OF CHLORHEXIDINA DIGLUCONATE AND NYSTATIN ON THE GROWING CONTROL OF Candida albicans Abstract The study aim to compare the antifungal properties between 100.000 IU nystatin and 0.2% chlorhexidine digluconate solution. The C. albicans suspension was seeded in a BHI agar culture, with 6mm of thickness distributed in Petri plates with 7mm diameters holes. After 10 repetitions, the results were measured according to the inhibitions halos created by the solution. The chlorhexidine solution inhibition halo was 27mm and the nystatin was 17mm. Therefore, the 0.2% chlorhexidine solution may be considered more effective them nystatin in the antifungal action against yeast strains.

scholarly journals Prothioconazole and Prothioconazole-Desthio Activities against Candida albicans Sterol 14-α-Demethylase

2012 ◽  
Vol 79 (5) ◽  
pp. 1639-1645 ◽  
Author(s):  
Josie E. Parker ◽  
Andrew G. S. Warrilow ◽  
Hans J. Cools ◽  
Bart A. Fraaije ◽  
John A. Lucas ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Type Ii ◽  
Inhibitor Binding ◽  
Binding Properties ◽  
Content Type ◽  
Azole Antifungals ◽  
A Cell ◽  
Antifungal Action ◽  
Binding Spectra

ABSTRACTProthioconazole is a new triazolinthione fungicide used in agriculture. We have usedCandida albicansCYP51 (CaCYP51) to investigate thein vitroactivity of prothioconazole and to consider the use of such compounds in the medical arena. Treatment ofC. albicanscells with prothioconazole, prothioconazole-desthio, and voriconazole resulted in CYP51 inhibition, as evidenced by the accumulation of 14α-methylated sterol substrates (lanosterol and eburicol) and the depletion of ergosterol. We then compared the inhibitor binding properties of prothioconazole, prothioconazole-desthio, and voriconazole with CaCYP51. We observed that prothioconazole-desthio and voriconazole bind noncompetitively to CaCYP51 in the expected manner of azole antifungals (with type II inhibitors binding to heme as the sixth ligand), while prothioconazole binds competitively and does not exhibit classic inhibitor binding spectra. Inhibition of CaCYP51 activity in a cell-free assay demonstrated that prothioconazole-desthio is active, whereas prothioconazole does not inhibit CYP51 activity. Extracts fromC. albicansgrown in the presence of prothioconazole were found to contain prothioconazole-desthio. We conclude that the antifungal action of prothioconazole can be attributed to prothioconazole-desthio.

scholarly journals Antifungal Effect of Silver Nitrate on Prosthodontic Dentures

2020 ◽  
Vol 24 (2) ◽  
pp. 96-101
Author(s):  
Mahmut Sertac Ozdogan ◽  
Mustafa Gumusok ◽  
Mihriban Yucel ◽  
Bedia Dinc ◽  
Muzaffer Mizrak ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Silver Nitrate ◽  
Sodium Hypochlorite ◽  
Acrylic Resin ◽  
Chlorhexidine Digluconate ◽  
Promising Alternative ◽  
Antifungal Effect ◽  
Colony Forming Unit ◽  
Colony Counting

SummaryBackground/Aim: Although there are studies about the antimicrobial activity of silver, there is no study evaluating it as a denture disinfectant. The purpose of this study was to explore the effectiveness of 6 disinfectant solutions (50% vinegar, 100% vinegar, 1% silver nitrate, 2% silver nitrate, %1 sodium hypochlorite, 0,12% chlorhexidine digluconate) in the disinfection of acrylic resin specimens contaminated in vitro by Candida albicans, as measured by residual colony-forming unit (CFU).Material and Methods: 66 pieces of 10mmx2mm acrylic resin disc samples were prepared and incubated in 1x106 cell/ml suspension of C. albicans ATCC 18804 for 24 h (one of them as a control, n=11/group). The specimens were then transferred into tubes containing 10 ml of the tested disinfectants and kept for 10 minutes in the disinfectant. After washing with saline, the specimens were vortexed to remove the microorganisms adhered to the surfaces. Colony counting of the collected microorganisms was performed on Sabouroud dextrose medium using 10−1, 10−2 and 10−3 dilutions. The results were analysed using Kruskal-Wallis and Mann-Whitney U tests (p<0,05).Results: The results showed that 1% sodium hypochlorite, %1 silver nitrate and 2% silver nitrate were the most effective against Candida Albicans (p<0,05), followed by 100% vinegar, 0,12% chlorhexidine digluconate and 50% vinegar (p<0,05).Conclusions: Within the boundaries of this study, we conclude that 1% silver nitrate is a promising alternative disinfectant to 1% sodium hypochlorite and performs better compared to 0,12 % chlorhexidine gluconate, 50% and 100% vinegar.

scholarly journals Novel Bi-Factorial Strategy against Candida albicans Viability Using Carnosic Acid and Propolis: Synergistic Antifungal Action

2020 ◽  
Vol 8 (5) ◽  
pp. 749
Author(s):  
Alejandra Argüelles ◽  
Ruth Sánchez-Fresneda ◽  
José P. Guirao-Abad ◽  
Cristóbal Belda ◽  
José Antonio Lozano ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Pathogenic Fungi ◽  
Carnosic Acid ◽  
Moderate Degree ◽  
Drastic Reduction ◽  
Fungicidal Action ◽  
Natural Extracts ◽  
Antifungal Action ◽  
High Concentrations

The potential fungicidal action of the natural extracts, carnosic acid (obtained from rosemary) and propolis (from honeybees’ panels) against the highly prevalent yeast Candida albicans, used herein as an archetype of pathogenic fungi, was tested. The separate addition of carnosic acid and propolis on exponential cultures of the standard SC5314 C. albicans strain caused a moderate degree of cell death at relatively high concentrations. However, the combination of both extracts, especially in a 1:4 ratio, induced a potent synergistic pattern, leading to a drastic reduction in cell survival even at much lower concentrations. The result of a mathematical analysis by isobologram was consistent with synergistic action of the combined extracts rather than a merely additive effect. In turn, the capacity of SC5314 cells to form in vitro biofilms was also impaired by the simultaneous presence of both agents, supporting the potential application of carnosic acid and propolis mixtures in the prevention and treatment of clinical infections as an alternative to antibiotics and other antifungal agents endowed with reduced toxic side effects.

scholarly journals In vitro susceptibility of oral Candida albicans strains to different pH levels and calcium hydroxide saturated aqueous solution

2012 ◽  
Vol 23 (3) ◽  
pp. 192-198 ◽  
Author(s):  
Paulo Henrique Weckwerth ◽  
Cristiane Carnietto ◽  
Ana Carolina Villas Boas Weckwerth ◽  
Marco Antonio Hungaro Duarte ◽  
Milton Carlos Kuga ◽  
...  
Keyword(s):  
Aqueous Solution ◽  
Candida Albicans ◽  
Oral Cavity ◽  
Calcium Hydroxide ◽  
Culture Broth ◽  
Saturated Aqueous Solution ◽  
Chi Square ◽  
Agar Culture ◽  
In Vitro Susceptibility

Candida albicans is present in the oral cavity and in the whole digestive tract of humans and other animals, being frequently related to endodontic treatment failure. The present study determined the incidence of C. albicans in the oral cavity and the susceptibility of isolates to different pH values and saturated calcium hydroxide aqueous solution at pH 12.5. Sixty-five patients attending the Endodontic Clinic at the Sagrado Coração University participated in the study. The collected samples were cultivated in selective media for C. albicans and the isolates were tested in terms of resistance to both alkaline pH and saturated aqueous solution of calcium hydroxide. In relation to time variables, yeast viability was assessed by the Sabouraud's agar culture and fluorescein diacetate and ethidium bromide fluorescent staining method. Results from the different pHs and experimental times, including those from different techniques measuring fungal viability, were compared using the chi-square and Fisher's exact tests (α=0.05). The yeasts became completely inviable after 48 h of contact with the calcium hydroxide solution. On the other hand, when exposed to the alkaline culture broth, the yeasts were found to be viable at pHs 9.5 and 10.5 for up to 7 days. In conclusion, C. albicans can only be completely inhibited by direct contact with saturated calcium hydroxide aqueous solution after 48 h of exposure.

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