scholarly journals INVESTIGAÇÃO FITOQUÍMICA E AVALIAÇÃO DA ATIVIDADE ANTIBACTERIANA DA Mikania lanuginosa DC (ASTERACEAE)

2002 ◽  
Vol 3 (2) ◽  
Author(s):  
R. Z. Da Silva ◽  
E. De M. RIOS ◽  
M. Z. Da SILVA ◽  
L. De F. Leal ◽  
R. A. YUNES ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Cereus ◽  
Staphylococcus Epidermidis ◽  
Dilution Method ◽  
Isolation And Purification ◽  
Kaurenoic Acid ◽  
Reported Data

O presente trabalho envolveu extração, isolamento, purificação e identificação dos constituintes químicos das partes aéreas da Mikania lanuginosa DC (ASTERACEAE). No processo de extração ocorreu a formação de um precipitado, no qual foi isolado e purificado um composto majoritário, que por análises espectroscópicas de IV, RMN 1H, RMN 13C e por comparação com os dados existentes na literatura, foi identificado como sendo o diterpeno ácido caurenóico. Foram realizados ensaios biológicos para avaliar a ação antimicrobiana com o precipitado, extrato etanólico bruto, as frações hexânica, diclorometano, acetato de etila, butanólica e o diterpeno ácido caurenóico contra as cepas Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228 e Bacillus cereus, pela técnica da difusão em meio sólido por cavidade e posteriormente pelo método de diluição em placa. Os resultados demonstraram que as frações hexânica, diclorometano, acetato de etila, butanólica e o ácido caurenóico obtidos da M. lanuginosa, apresentaram atividade antimicrobiana moderada frente as bactérias Gram positivas e foram inativos contra as bactérias Gram negativas. Phytochemical Investigation and Antimicrobial Activity Evaluation of the Mikania lanuginosa DC (ASTERACEAE) Abstract The present study investigated the extraction, isolation and purification the chemical compounds of the aerial parts of Mikania lanuginosa DC (ASTERACEAE). In the process of extraction it occurred the formation of a precipitate, it which was isolated and purified a ruling compound, and by spectroscopic analysis of the IR, 1H NMR, 13C NMR and comparison with reported data in the literature it was identified as being the diterpene kaurenoic acid. It were performed biological assays to estimate the antimicrobial action with the precipited, crude etanolic extract, hexane, dichoromethane, acetate ethyl, butane fractions and diterpene kaurenoic acid contrasting with strains Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228 e Bacillus cereus, by technique of the diffusion in solid mean by cavity and afterwards by the plaque dilution method. The results showed that the hexane, dichoromethane, acetate ethyl, butane fractions and the kaurenoic acid obtained from the Mikania lanuginosa DC presented a moderate antimicrobial activity face to Gram positives bacteria and got inert against the Gram negatives bacteria.

scholarly journals INVESTIGAÇÃO DA ATIVIDADE ANTIBACTERIANA DE Calendula officinalis L. (ASTERACEAE)

2001 ◽  
Vol 2 (1) ◽  
Author(s):  
Ana Marcia De Matos Volpato ◽  
Ester De Moura Rios ◽  
Marilis Dallarmi Miguel ◽  
Paulo Cesar Sander ◽  
Obdúlio Gomes Miguel
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Staphylococcus Epidermidis ◽  
Antibacterial Action ◽  
Dilution Method ◽  
Calendula Officinalis ◽  
Gram Negative ◽  
Solid Media ◽  
Calendula Officinalis L

A atividade antimicrobiana de Calendula officinalis L. (Asteraceae) já foi cientificamente comprovada em outros países. O objetivo deste trabalho foi avaliar a atividade antibacteriana de C. officinalis cultivada no Paraná - Brasil, nas condições de extração etanólica 50%, com filtração e partições sucessivas com hexano, diclorometano, acetato de etila e butanol, frente a cepas Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228 e Bacillus cereus, pela técnica da difusão em meio sólido por cavidade e posteriormente pelo método de diluição em placa. Os resultados demonstraram que algumas frações do extrato inibiram as bactérias gram-positivas, com atividade frente a Staphylococcus e Bacilos e inexiste em gram negativas, indicando que o princípio ativo responsável pela atividade antibacteriana observada possa ser isolado a partir das frações Hexânica e DCM nas condições de extração propostas neste trabalho. Abstract The Calendula officinalis L. (Asteraceae) antimicrobial action has already been scientifically proved in other countries. The aim of this study was to assess the antibacterial action of the Callendula officinalis which is grown in Paraná - Brasil, under extraction conditions at ethanol 50%, with filtration and successive partitions with hexane, dichloromethane, ethyl acetate and butane, contrasting with strains Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228 and Bacilus cereus, by the difusion technique in solid media by cavity and afterwards by the plaque dilution method. The results showed that some fractions of the extract inhibited the gram-prositive bacteria, with activity in contrast with Staphylococcus and Bacillus and non-existing in gram-negative, also indicating that the active ingredient responsible for the antibacterial action observed can be isolated from the Hexanic and DCM fractions under the extraction conditions proposed in this study.

scholarly journals Investigation of the Antimicrobial Activity of Rhaponticum (Rhaponticum Carthamoides D.C. Iljin) and Shrubby Cinquefoil (Potentilla Fruticosa L.)

Medicina ◽  
2011 ◽  
Vol 47 (3) ◽  
pp. 24 ◽  
Author(s):  
Vilma Jurkštienė ◽  
Alvydas Pavilonis ◽  
Daiva Garšvienė ◽  
Algirdas Juozulynas ◽  
Laimutė Samsonienė ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Candida Albicans ◽  
Klebsiella Pneumoniae ◽  
Bacillus Cereus ◽  
Potentilla Fruticosa ◽  
Rhaponticum Carthamoides

The aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. Material and Methods. Ethanol extract from the leaves of rhaponticum (Rhaponticum carthamoides D.C. Iljin) and shrubby cinquefoil (Potentilla fruticosa L.) was produced at the Department of Food Technology, Kaunas University of Technology. The antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria Staphylococcus aureus ATCC 25923, Enterococcus faecalis ATCC 29212, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 33499, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 12459, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 8035 and fungi Candida albicans ATCC 60193). The minimum inhibitory concentration (MIC) of the examined preparations was determined. Results. Both studied preparations – rhaponticum (Rhaponticum carthamoides D.C. Iljin) and shrubby cinquefoil (Potentilla fruticosa L.) – demonstrated similar antimicrobial activity. The highest sensitivity to the studied preparations was observed in microbes with eukaryotic cell structure: Candida albicans, which is a fungus, and a spore-forming prokaryotic bacterium, Bacillus cereus. The highest resistance was observed in Escherichia coli and Klebsiella pneumoniae. Conclusions. The studied preparations – viscous extracts of rhaponticum and shrubby cinquefoil – are substances with antimicrobial activity against gram-positive (Staphylococcus aureus and Enterococcus faecalis) and gram-negative (Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Proteus mirabilis) bacteria, spore-forming bacteria (Bacillus subtilis and Bacillus cereus), and fungi (Candida albicans).

The Antimicrobial Activity of Extracts of the Lichen Cetraria aculeata and Its Protolichesterinic Acid Constituent

2003 ◽  
Vol 58 (11-12) ◽  
pp. 850-854 ◽  
Author(s):  
Ayşen Özdemir Türk ◽  
Meral Yılmaz ◽  
Merih Kıvanç ◽  
Hayrettin Türk
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bacillus Subtilis ◽  
Listeria Monocytogenes ◽  
Bacillus Cereus ◽  
Aeromonas Hydrophila ◽  
Proteus Vulgaris ◽  
Ethanol Extracts

Abstract In this study, the antimicrobial activity of the acetone, diethyl ether and ethanol extracts of the lichen Cetraria aculeata has been investigated. The extracts were tested against twelve bacteria and eight fungi and found active against Escherichia coli, Staphylococcus aureus, Aeromonas hydrophila, Proteus vulgaris, Streptococcus faecalis, Bacillus cereus, Bacillus subtilis, Pseudomonas aeruginosa, Listeria monocytogenes. No antimicrobial activity against the fungi was detected. It was determined that only one substance in the extracts has antimicrobial activity and it was characterized as protolichesterinic acid. The MICs of the extracts and protolichesterinic acid were also determined.

Chemical Composition and Antimicrobial Activity of Salvia x jamensis Essential Oil

2012 ◽  
Vol 7 (9) ◽  
pp. 1934578X1200700 ◽  
Author(s):  
Daniele Fraternale ◽  
Guido Flamini ◽  
Angela Bisio ◽  
Maria Cristina Albertini ◽  
Donata Ricci
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Bacillus Cereus ◽  
Enterobacter Cloacae ◽  
Caryophyllene Oxide ◽  
First Report

This is the first report about the composition of the essential oil of Salvia x jamensis. The major compounds identified by GC-MS were β-caryophyllene (14.8 %), β-pinene (6.8 %), caryophyllene oxide (6.0 %), δ-cadinene (5.5 %), α-pinene (5.2 %) and spatulenol (5.2 %). The oil showed antimicrobial activity against three Gram (+) bacteria: Bacillus cereus, Staphylococcus aureus, and S. epidermidis, and three Candida strains: C. albicans, C. glabrata and C. tropicalis, while it was inactive against three Gram (-) bacteria: Pseudomonas aeruginosa, Escherichia coli and Enterobacter cloacae.

scholarly journals A Time-Kill Assay Study on the Synergistic Bactericidal Activity of Pomegranate Rind Extract and Zn (II) against Methicillin-Resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1889
Author(s):  
Amal Alrashidi ◽  
Mohammed Jafar ◽  
Niamh Higgins ◽  
Ciara Mulligan ◽  
Carmine Varricchio ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Bactericidal Activity ◽  
Staphylococcus Epidermidis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Synergistic Activity ◽  
Methicillin Resistant ◽  
Time Kill

There is a need for new antimicrobial systems due to increased global resistance to current antimicrobials. Pomegranate rind extract (PRE) and Zn (II) ions both possess a level of antimicrobial activity and work has previously shown that PRE/Zn (II) in combination possesses synergistic activity against Herpes simplex virus and Micrococcus luteus. Here, we determined whether such synergistic activity extended to other, more pathogenic, bacteria. Reference strains of methicillin-resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa were cultured and subjected to challenge by PRE, Zn (II), or PRE + Zn (II), in time-kill assays. Data were obtained independently by two researchers using different PRE preparations. Statistically significant synergistic activity for PRE + Zn (II) was shown for all four bacterial strains tested compared to untreated controls, although the extent of efficacy and timescales varied. Zn (II) exerted activity and at 1 h, it was not possible to distinguish with PRE + Zn (II) combination treatment in all cases. PRE alone showed low activity against all four bacteria. Reproducible synergistic bactericidal activity involving PRE and Zn (II) has been confirmed. Potential mechanisms are discussed. The development of a therapeutic system that possesses demonstrable antimicrobial activity is supported which lends itself particularly to topical delivery applications, for example MRSA infections.

Two Epimeric Flavalignans from Trichilia catigua (Meliaceae) with Antimicrobial Activity

2002 ◽  
Vol 57 (5-6) ◽  
pp. 483-488 ◽  
Author(s):  
Moacir G. Pizzolatti ◽  
Andreia F. Venson ◽  
Artur Smânia Júnior ◽  
Elza de F. A. Smânia ◽  
Raimundo Braz-Filho
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Antimicrobial Activity ◽  
Antibacterial Activity ◽  
Natural Products ◽  
Bacillus Cereus ◽  
Spectroscopic Data ◽  
2D Nmr ◽  
And Staphylococcus Aureus

A mixture of flavalignan cinchonains Ia and Ib was isolated from the bark of Trichilia catigua. The structures were established on the basis of spectroscopic data of the natural products and their methylated derivatives including 2D NMR experiments, and compared with data in the literature. These flavalignans exhibited antibacterial activity against Bacillus cereus, Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus.

scholarly journals SKIRTINGŲ SUDĖČIŲ KONTAKTINIŲ LĘŠIŲ TIRPALŲ ANTIMIKROBINĖS SAVYBĖS

2019 ◽  
Vol 28 (6) ◽  
pp. 74-80
Author(s):  
Žaneta Maželienė ◽  
Ieva Dumšytė ◽  
Jolita Kirvaitienė ◽  
Ingrida Viliušienė
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Bacillus Cereus ◽  
Staphylococcus Epidermidis

Įvadas. Akies infekcijos rizika dažnėja didėjant kon­taktinių lęšių nešiotojų skaičiui. Kontaktinių lęšių tirpalai – priemonės, skirtos kontaktinių lęšių skala­vimui, nešvarumų valymui, apnašų šalinimui, dezin­fekcijai (mikroorganizmų sunaikinimui), drėkinimui bei laikymui. Kontaktinių lęšių tirpalų sudėtis nule­mia skirtingas jų antimikrobines savybės. Tyrimo tikslas. Nustatyti kontaktinių lęšių skysčių antimikrobinį veikimą skirtinguose jų poveikio eta­loninėms bakterijų kultūroms laiko intervaluose. Tyrimo medžiaga ir metodai. Mikrobiologinio tyrimo metu nustatytas kontaktinių lęšių tirpalų antimikro­binis poveikis bakterijoms – Staphylococcus aureus ATCC 25923, Staphylococcus epidermidis ATCC 12228, Escherichia coli ATCC 25922, Klebsiella pneumoniae ATCC 13883, Pseudomonas aeruginosa ATCC 27853, Bacillus cereus ATCC 11778. Bakte­rijų kultūros buvo veikiamos skirtingais kontaktinių lęšių tirpalais 0,5 val., 1 val., 2 val., 4 val., 6 val., 8 val. ir po to nustatytas likęs gyvybingų bakterijų kie­kis tiriamajame tirpale. Rezultatai. Kontaktinių lęšių tirpalų antimikrobines savybes lemia kelių sudedamųjų dalių sinergistinis antimikrobinis poveikis dažniausiai akims pavojin­giems infekcinių ligų sukėlėjams. Silpniausiai bak­terijas veikė poliheksametilenbiguanido 0,0001 proc. tirpalas. Poliheksametilenbiguanido tirpalo antimi­krobinis poveikis stiprėjo didėjant jo koncentraci­jai ir į tirpalą pridėjus ramunėlių ar alavijo. Polihek­sametilenbiguanido 0,0002 proc. tirpalas su alaviju ir kontaktinių lęšių tirpalas su boro rūgštimi veikia panašiai, kaip ir poliheksametilenbiguanido 0,0002 proc. tirpalas su alaviju, tačiau visos bakterijos buvo sunaikintos po 6-8 val. poveikio. Stipriausią antimi­krobinį poveikį turėjo kontaktinių lęšių tirpalas su 3 proc. vandenilio peroksido, kuris per 30 min. – 1 val. sunaikino visas tirtas bakterijas. Išvados. Akių infekcijos galima išvengti naudojant kontaktinių lęšių poliheksametilenbiguanido 0,0002 proc. tirpalą su alaviju ar kontaktinių lęšių tirpalus, kurių sudėtyje yra 3 proc. vandenilio peroksido. Lę­šius tirpaluose reikia laikyti 2 – 4 val.

scholarly journals Atividade antibacteriana de extratos de folhas de Montrichardia linifera (Arruda) Schott (Araceae)

2015 ◽  
Vol 17 (4 suppl 3) ◽  
pp. 1142-1149 ◽  
Author(s):  
J.A.L MIRANDA ◽  
J.A. ROCHA ◽  
K.M. ARAÚJO ◽  
P.V. QUELEMES ◽  
S.J. MAYO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Klebsiella Pneumoniae ◽  
Enterococcus Faecalis ◽  
Staphylococcus Epidermidis

RESUMO O uso de plantas medicinais no tratamento de doenças é uma estratégia antiga utilizada por praticamente todas as populações do mundo, e, embora novos antibióticos tenham sido desenvolvidos para o controle de micro-organismos infecciosos, às vezes são ineficazes. Diversos extratos de plantas medicinais têm efeitos antimicrobianos, principalmente quando associados à antibióticos de uso clínico, representando alternativa terapêutica para doenças infecciosas. Montrichardia linifera, conhecida popularmente como aninga, é espécie macrófita, aquática emergente de hábito herbáceo, pertencente a família Araceae e ocorre em áreas alagáveis. A utilidade farmacológica desta espécie é diversificada tendo sido relatada como cicatrizante, antirreumático, antidiurético e expectorante. Devido à relevância no campo etnofarmacológico, ampla utilização na medicina popular e escassez de trabalhos relacionados à atividade antibacteriana desta espécie, objetivou-se com este trabalho avaliar a atividade antibacteriana de extratos alcoólicos de folhas de Montrichardia linifera, coletadas na margem do rio Igaraçu, Parnaíba-PI. O extrato foi testado em oito cepas de bactérias: Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Escherichia coli, Klebsiella pneumoniae e Pseudomonas aeruginosa. Foram utilizadas as técnicas de verificação da formação de halos de inibição e determinação das concentrações inibitórias e bactericidas mínimas. Os testes antibacterianos evidenciaram como principais resultados que o extrato metanólico seco (EMS), extrato metanólico fresco (EMF), e o extrato etanólico seco (EES), apresentaram ação antibacteriana, enquanto o extrato etanólico fresco (EEF) não apresentou atividade para as bactérias testadas. O EMS foi o mais eficiente, inibindo o crescimento bacteriano na concentração de 200 μg/mL para E. faecalis, 400 μg/mL para S. aureus, 400 μg/mL para S. epidermidis e 2.000 μg/mL para P. aeruginosa. O EMF obteve CIM de 2.000 μg/mL para E. faecalis e EES obteve CIM de 250 μg/mL para E. faecalis. Os resultados demonstraram que M. linifera constitui fonte eficiente de compostos bioativos antibacterianos. Os estudos sobre as propriedades farmacológicas de plantas da família Araceae são escassos, e os resultados deste trabalho são pioneiros em relação a atividade antibacteriana desta espécie.

scholarly journals Synthesis of CoFe 2 O 4 /BiOI Nanocomposite: Effects on Staphylococcus aureus, Escherichia coli, Pseudomonas Aeruginosa and Bacillus Cereus

2021 ◽  
Author(s):  
Davood Gheidari ◽  
Morteza Mehrdad ◽  
Saloomeh Maleki ◽  
Samanesadat Hosseini
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Pseudomonas Aeruginosa ◽  
Bacillus Cereus ◽  
Antibacterial Properties ◽  
Limited Area ◽  
X Ray Diffraction ◽  
X Ray ◽  
Inhibition Concentration ◽  
Resolution Mapping

Abstract With the increase of general knowledge and the advancement of science and technology, antibacterial substances were used more than antibiotics. In our current study, the antibacterial virtues of CFO/BiOI nanocomposite were investigated due to its high importance on Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Bacillus cereus. MIC, MBC , Disk Diffusion and IC50 tests Cefalotin (CF), Amoxicillin (AMX), Gentamicin (GM), Trimethoprim-sulphamethoxazole (SXT) and Ceftriaxone (CRO) antibiotics in concentration 30W, 10i, 10t , 25h and 30 were used to find the antibacterial properties of the synthesized nanocomposite, respectively. For the synthesis of nanocomposites polyethylene glycol (PEG) and sulfonic acid was used as a solvent. It is noteworthy that the synthesis was performed by heat dissolution method without the presence of surfactant. Also, various techniques such as X-Ray Diffraction(XRD), Scanning Electron Microscope (SEM), High resolution mapping and Energy Dispersive X-ray spectroscopy (EDAX) have been used to determine the properties of produced nanocomposites. SEM test results showed that the formed nanoparticles were globular and their size was limited area of 22 to 34 nm. The results showed CFO / BiOI nanocomposite exhibits strong significant biological activity against Bacillus cereus. The results of MBC (Minimum Bactericidal Concentration) and MIC (Minimum Inhibition Concentration) tests for CFO/BiOI nanocomposites on bacteria were examined in the range of 0.12-0.48 mg/ml and 0.06 to 0.24 mg/ml respectively. According to the results, the minimum IC50 value was determined at a concentration of 0.061 mg/ml. On the other hand, the most resisting and susceptible bacteria in this method were Pseudomonas aeruginosa and Bacillus cereus, respectively. These findings are identical to those of a prior study on CoFe2O4 nanoparticles antibacterial properties. MBC of the nanocomposites, 50 µl from all the tubes that showed no obvious bacterial growth were distributed on BHI agar plates and incubated for 24 h at 37 ◦C. The MBC endpoint is defined as the lowest concentration which killed 98% of the bacterial population.

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