Allele-specific PCR Markers for Distinguishing High Molecular Weight Glutenin Subunit Dtx5 of Aegilops tauschii from Dx5 of Common Wheat

2018 ◽  
Author(s):  
Hongshen Wan ◽  
Jipeng Qu ◽  
Jun Li ◽  
Zhefeng Zhang ◽  
Jianmin Zheng ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Common Wheat ◽  
Aegilops Tauschii ◽  
Glutenin Subunit ◽  
Pcr Markers ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

scholarly journals Characterization of a Novel High-Molecular-Weight Glutenin Subunit Pair 2.6 + 12 in Common Wheat Landraces in the Xinjiang Uygur Autonomous District of China

2007 ◽  
Vol 57 (3) ◽  
pp. 253-255 ◽  
Author(s):  
Hua Cong ◽  
Kanenori Takata ◽  
Tatsuya M. Ikeda ◽  
Mikiko Yanaka ◽  
Hiroshi Fujimaki ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Common Wheat ◽  
Glutenin Subunit ◽  
Autonomous District ◽  
Wheat Landraces

scholarly journals Development of diagnostic PCR and LAMP markers for MALE STERILITY 1 (MS1) in Cryptomeria japonica D. Don

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Yoichi Hasegawa ◽  
Saneyoshi Ueno ◽  
Fu-Jin Wei ◽  
Asako Matsumoto ◽  
Tokuko Ujino-Ihara ◽  
...  
Keyword(s):  
Male Sterility ◽  
Cryptomeria Japonica ◽  
Lamp Method ◽  
Pcr Markers ◽  
Causative Gene ◽  
Laboratory Equipment ◽  
Diagnostic Pcr ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

Abstract Objective Due to the allergic nature of the pollen of Cryptomeria japonica, the most important Japanese forestry conifer, a pollen-free cultivar is preferred. Mutant trees detected in nature have been used to produce a pollen-free cultivar. In order to reduce the time and cost needed for production and breeding, we aimed to develop simple diagnostic molecular markers for mutant alleles of the causative gene MALE STERILITY 1 (MS1) in C. japonica to rapidly identify pollen-free mutants. Results We developed PCR and LAMP markers to detect mutant alleles and to present experimental options depending on available laboratory equipment. LAMP markers were developed for field stations, where PCR machines are unavailable. The LAMP method only needs heat-blocks or a water bath to perform the isothermal amplification and assay results can be read by the naked eye. Because the causative mutations were deletions, we developed two kinds of PCR markers, amplified length polymorphism (ALP) and allele specific PCR (ASP) markers. These assays can be visualized using capillary or agarose gel electrophoresis.

scholarly journals Development of diagnostic PCR and LAMP markers for MALE STERILITY 1 (MS1) in Cryptomeria japonica D. Don

2020 ◽  
Author(s):  
Yoichi Hasegawa ◽  
Saneyoshi Ueno ◽  
Fu-Jin Wei ◽  
Asako Matsumoto ◽  
Tokuko Ujino-Ihara ◽  
...  
Keyword(s):  
Male Sterility ◽  
Cryptomeria Japonica ◽  
Agarose Gel Electrophoresis ◽  
Pcr Markers ◽  
Causative Gene ◽  
Laboratory Equipment ◽  
Diagnostic Pcr ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr

AbstractObjectiveDue to the allergic nature of the pollen of Cryptomeria japonica, the most important Japanese forestry conifer, a pollen-free cultivar is preferred. Mutant trees detected in nature have been used for the production of a pollen-free cultivar. In order to reduce the time and cost needed for the production and breeding, we aimed to develop simple diagnostic molecular markers for mutant alleles of the causative gene MALE STERILITY 1 (MS1) in C. japonica. The expected function of this gene, its two dysfunctional mutations, and genetic diversity were described recently in a related study.ResultsWe have developed PCR and LAMP markers to detect mutant alleles and to present experimental options depending on available laboratory equipment. At field stations, where PCR machines are not available, LAMP markers were developed. LAMP only needs heat-blocks or a water bath to perform the isothermal amplification and assay results can be easily seen by eye. Because the causative mutations were deletions, two kinds of PCR markers, amplified length polymorphism (ALP) and allele specific PCR (ASP) markers, were developed. These assays can be carried out by capillary or agarose gel electrophoresis.

Development of Allele-specific PCR Markers for Tm22 Gene In Tomato

2013 ◽  
Vol 13 (1) ◽  
pp. 34-39
Author(s):  
Feng-Mei Jin ◽  
Xue Jun ◽  
Song Jian ◽  
Zhong-Qi Liu ◽  
Xue Lin
Keyword(s):  
Pcr Markers ◽  
Specific Pcr ◽  
Allele Specific ◽  
Allele Specific Pcr
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