Preparation and Characteristics of Enzymatic Reaction Systems Using Lipid Membranes and Fine Droplets of Liposomes

MEMBRANE ◽  
2021 ◽  
Vol 46 (2) ◽  
pp. 78-83
Author(s):  
Makoto Yoshimoto
2018 ◽  
Vol 119 ◽  
pp. 453-461 ◽  
Author(s):  
Danli Wang ◽  
Lufeng Yan ◽  
Xiaobin Ma ◽  
Wenjun Wang ◽  
Mingming Zou ◽  
...  

Talanta ◽  
2008 ◽  
Vol 75 (3) ◽  
pp. 666-670 ◽  
Author(s):  
Jianlong Wang ◽  
Fuan Wang ◽  
Hongjun Chen ◽  
Xiaohua Liu ◽  
Shaojun Dong

2020 ◽  
Author(s):  
Kelath Murali Manoj ◽  
Daniel Andrew Gideon ◽  
Abhinav Parashar ◽  
Deepak Haarith ◽  
Afsal Manekkathodi

Murburn concept is a new redox metabolic paradigm which advocates that several redox enzymes generate/stabilize diffusible reactive (oxygen) species (DRS or DROS) to carry out useful electron/moiety transfer reactions at biological membrane interfaces (Manoj 2020a). Herein, we show that the components and principles of redox reactions within chloroplasts/cyanobacteria share several similarities with soluble and simple extracellular or peroxisomal heme-enzymes that carry out electron/group transfer. We explore the comparison in detail with membrane-embedded and complex systems that catalyze: (i) microsomal xenobiotic metabolism and (ii) mitochondrial oxidative phosphorylation. We point out that the murburn interpretations of catalytic phenomena are consistent through the various reaction systems cited above. Further, we argue that evolutionary constraints and the physiological restrictions of neutral pH ranges discount proton-gradient based explanations for bioenergetic phosphorylations in chloroplasts. Therefore, we propose that the highly packed thylakoid membranes with minute aqueous volumes serve to enhance the lifetimes of oxygen-centered radicals and intermediates. The murburn perspective could also potentially explain protein supercomplexes in chloroplasts, and generation of ATP in mitochondria by photo-activation. Our proposal also highlights the evolutionary significance of lipid membranes and utility of oxygen in diverse life processes.


1973 ◽  
Vol 15 (6) ◽  
pp. 1131-1145 ◽  
Author(s):  
D. D. Bruns ◽  
J. E. Bailey ◽  
D. Luss

1997 ◽  
Vol 161 ◽  
pp. 437-442
Author(s):  
Salvatore Di Bernardo ◽  
Romana Fato ◽  
Giorgio Lenaz

AbstractOne of the peculiar aspects of living systems is the production and conservation of energy. This aspect is provided by specialized organelles, such as the mitochondria and chloroplasts, in developed living organisms. In primordial systems lacking specialized enzymatic complexes the energy supply was probably bound to the generation and maintenance of an asymmetric distribution of charged molecules in compartmentalized systems. On the basis of experimental evidence, we suggest that lipophilic quinones were involved in the generation of this asymmetrical distribution of charges through vectorial redox reactions across lipid membranes.


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