Image Analysis of Membrane Receptors in Living Cells by Coiled-coil Labeling Method

MEMBRANE ◽  
2013 ◽  
Vol 38 (2) ◽  
pp. 82-86
Author(s):  
Yoshiaki Yano ◽  
Katsumi Matsuzaki
Keyword(s):  
Image Analysis ◽  
Coiled Coil ◽  
Living Cells ◽  
Membrane Receptors ◽  
Labeling Method

Coiled-Coil Tag−Probe System for Quick Labeling of Membrane Receptors in Living Cells

2008 ◽  
Vol 3 (6) ◽  
pp. 341-345 ◽  
Author(s):  
Yoshiaki Yano ◽  
Akiko Yano ◽  
Shinya Oishi ◽  
Yukihiko Sugimoto ◽  
Gozoh Tsujimoto ◽  
...  
Keyword(s):  
Coiled Coil ◽  
Living Cells ◽  
Membrane Receptors ◽  
Probe System

scholarly journals A Visualization Tool for Oligomerization and Internalization of Membrane Proteins in Living Cells: Coiled-Coil Labeling Method

2014 ◽  
Vol 134 (4) ◽  
pp. 501-506 ◽  
Author(s):  
Yoshiaki Yano ◽  
Kenichi Kawano ◽  
Kaoru Omae ◽  
Yuki Takeda ◽  
Sayaka Matsuzaki ◽  
...  
Keyword(s):  
Membrane Proteins ◽  
Coiled Coil ◽  
Living Cells ◽  
Visualization Tool ◽  
Labeling Method

scholarly journals How the biomimetic assembly of membrane receptors into multivalent domains is regulated by a small ligand

2021 ◽  
Author(s):  
Anna Grochmal ◽  
Ben Woods ◽  
Lilia Milanesi ◽  
Manuel Perez-Soto ◽  
Salvador Tomas
Keyword(s):  
Environmental Changes ◽  
Living Cells ◽  
Membrane Receptors

In living cells, communication requires the action of membrane receptors that are activated following very small environmental changes. A binary all-or-nothing behavior follows, making the organism extremely efficient at responding...

scholarly journals Imaging of persistent cAMP signaling by internalized G protein-coupled receptors

2010 ◽  
Vol 45 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Davide Calebiro ◽  
Viacheslav O Nikolaev ◽  
Martin J Lohse
Keyword(s):  
G Protein ◽  
Intracellular Signaling ◽  
Current Model ◽  
Living Cells ◽  
G Protein Coupled Receptors ◽  
Membrane Receptors ◽  
Optical Methods ◽  
Camp Signaling ◽  
Gpcr Signaling ◽  
G Protein Coupled

G protein-coupled receptors (GPCRs) are the largest family of plasma membrane receptors. They mediate the effects of several endogenous cues and serve as important pharmacological targets. Although many biochemical events involved in GPCR signaling have been characterized in great detail, little is known about their spatiotemporal dynamics in living cells. The recent advent of optical methods based on fluorescent resonance energy transfer allows, for the first time, to directly monitor GPCR signaling in living cells. Utilizing these methods, it has been recently possible to show that the receptors for two protein/peptide hormones, the TSH and the parathyroid hormone, continue signaling to cAMP after their internalization into endosomes. This type of intracellular signaling is persistent and apparently triggers specific cellular outcomes. Here, we review these recent data and explain the optical methods used for such studies. Based on these findings, we propose a revision of the current model of the GPCR–cAMP signaling pathway to accommodate receptor signaling at endosomes.

Volumetric Changes in Cells During Freezing and Thawing

1980 ◽  
Vol 102 (2) ◽  
pp. 91-97 ◽  
Author(s):  
J. M. Knox ◽  
G. S. Schwartz ◽  
K. R. Diller
Keyword(s):  
Experimental Data ◽  
Image Analysis ◽  
Close Agreement ◽  
Living Cells ◽  
Freezing Process ◽  
Freezing And Thawing ◽  
Image Analysis Technique ◽  
Computerized Image Analysis ◽  
Analysis Technique ◽  
Thawing Process

A thermodynamic model is presented to describe the combined freezing and thawing process for living cells. Continuous changes in the cell volume are predicted according to the thermal protocol imposed on the system. Experimental verification of the model is sought by monitoring continuously the volume of cells as frozen on a cryomicroscope. The volumes of individual cells are measured from sequential photomicrographs by a computerized image analysis technique. The model and experimental data are in quite close agreement for the freezing process, but upon thawing the experimentally measured volumes consistently increased much more rapidly than predicted by the model. The model can be made to conform to the data by accounting for a substantial influx of electrolyte to the cell at subfreezing temperatures.

scholarly journals Ligand-directed dibromophenyl benzoate chemistry for rapid and selective acylation of intracellular natural proteins

2015 ◽  
Vol 6 (5) ◽  
pp. 3217-3224 ◽  
Author(s):  
Yousuke Takaoka ◽  
Yuki Nishikawa ◽  
Yuki Hashimoto ◽  
Kenta Sasaki ◽  
Itaru Hamachi
Keyword(s):  
Living Cells ◽  
Protein Labeling ◽  
Natural Protein ◽  
Protein Imaging ◽  
Selective Acylation ◽  
Labeling Method

A rapid and selective protein labeling method, LDBB chemistry is a useful tool for natural protein imaging in living cells.

scholarly journals Detection of Oligomerization of Membrane Proteins using Coiled-Coil Tag-Probe Labeling Method and In-Cell Fluorescence Spectroscopy

2013 ◽  
Vol 104 (2) ◽  
pp. 42a
Author(s):  
Kenichi Kawano ◽  
Kaoru Omae ◽  
Sayaka Matsuzaki ◽  
Yoshiaki Yano ◽  
Katsumi Matsuzaki
Keyword(s):  
Membrane Proteins ◽  
Fluorescence Spectroscopy ◽  
Coiled Coil ◽  
Labeling Method
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