Proteomic characterization of oyster shell organic matrix proteins

2015 ◽  
Author(s):  
Abhishek Upadhyay
Keyword(s):  
Oyster Shell ◽  
Organic Matrix ◽  
Matrix Proteins ◽  
Shell Organic Matrix

scholarly journals Proteomic characterization of oyster shell organic matrix proteins (OMP)

2016 ◽  
Vol 12 (05) ◽  
pp. 266-278 ◽  
Author(s):  
Abhishek Upadhyay ◽  
◽  
Vengatesen Thiyagarajan ◽  
◽  
◽  
...  
Keyword(s):  
Oyster Shell ◽  
Organic Matrix ◽  
Matrix Proteins ◽  
Shell Organic Matrix

scholarly journals The ‘Shellome’ of the Crocus Clam Tridacna crocea Emphasizes Essential Components of Mollusk Shell Biomineralization

2021 ◽  
Vol 12 ◽  
Author(s):  
Takeshi Takeuchi ◽  
Manabu Fujie ◽  
Ryo Koyanagi ◽  
Laurent Plasseraud ◽  
Isabelle Ziegler-Devin ◽  
...  
Keyword(s):  
Organic Matrix ◽  
The Other ◽  
Matrix Proteins ◽  
Shell Formation ◽  
Molluscan Shell ◽  
Shell Matrix ◽  
Essential Components ◽  
Shell Matrix Proteins ◽  
Shell Organic Matrix ◽  
Tridacna Crocea

Molluscan shells are among the most fascinating research objects because of their diverse morphologies and textures. The formation of these delicate biomineralized structures is a matrix-mediated process. A question that arises is what are the essential components required to build these exoskeletons. In order to understand the molecular mechanisms of molluscan shell formation, it is crucial to identify organic macromolecules in different shells from diverse taxa. In the case of bivalves, however, taxon sampling in previous shell proteomics studies are focused predominantly on representatives of the class Pteriomorphia such as pearl oysters, edible oysters and mussels. In this study, we have characterized the shell organic matrix from the crocus clam, Tridacna crocea, (Heterodonta) using various biochemical techniques, including SDS-PAGE, FT-IR, monosaccharide analysis, and enzyme-linked lectin assay (ELLA). Furthermore, we have identified a number of shell matrix proteins (SMPs) using a comprehensive proteomics approach combined to RNA-seq. The biochemical studies confirmed the presence of proteins, polysaccharides, and sulfates in the T. crocea shell organic matrix. Proteomics analysis revealed that the majority of the T. crocea SMPs are novel and dissimilar to known SMPs identified from the other bivalve species. Meanwhile, the SMP repertoire of the crocus clam also includes proteins with conserved functional domains such as chitin-binding domain, VWA domain, and protease inhibitor domain. We also identified BMSP (Blue Mussel Shell Protein, originally reported from Mytilus), which is widely distributed among molluscan shell matrix proteins. Tridacna SMPs also include low-complexity regions (LCRs) that are absent in the other molluscan genomes, indicating that these genes may have evolved in specific lineage. These results highlight the diversity of the organic molecules – in particular proteins – that are essential for molluscan shell formation.

Regulation of in vitro and in vivo CaCO3 crystallization by fractions of oyster shell organic matrix

1988 ◽  
Vol 98 (1) ◽  
pp. 71-80 ◽  
Author(s):  
A. P. Wheeler ◽  
K. W. Rusenko ◽  
D. M. Swift ◽  
C. S. Sikes
Keyword(s):  
Oyster Shell ◽  
Organic Matrix ◽  
Shell Organic Matrix

scholarly journals Hemolysis in the spleen drives erythrocyte turnover

Blood ◽  
2020 ◽  
Author(s):  
Thomas robert leon Klei ◽  
Jill Jasmine Dalimot ◽  
Benjamin Nota ◽  
Martijn Veldthuis ◽  
Erik Mul ◽  
...  
Keyword(s):  
Molecular Mechanisms ◽  
Matrix Proteins ◽  
Human Spleen ◽  
Erythrocyte Adhesion ◽  
Subsequent Degradation ◽  
Macrophage Subpopulations ◽  
Senescent Erythrocytes ◽  
Red Pulp

Red pulp macrophages of the spleen mediate turnover of billions of senescent erythrocytes per day. However, the molecular mechanisms involved in sequestration of senescent erythrocytes, their recognition and their subsequent degradation by red pulp macrophages remain unclear. In this study we provide evidence that the splenic environment is of substantial importance in facilitating erythrocyte turnover through induction of hemolysis. Upon isolating human spleen red pulp macrophages we noted a substantial lack of macrophages that were in the process of phagocytosing intact erythrocytes. Detailed characterization of erythrocyte and macrophage subpopulations from human spleen tissue led to the identification of erythrocytes that are devoid of hemoglobin, so-called erythrocyte ghosts. By in vivo imaging and transfusion experiments we further confirmed that senescent erythrocytes that are retained in the spleen are subject to hemolysis. Additionally, we show that erythrocyte adhesion molecules, which are specifically activated on aged erythrocytes, cause senescent erythrocytes to interact with extracellular matrix proteins that are exposed within the splenic architecture. Such adhesion molecule-driven retention of senescent erythrocytes, under low shear conditions, was found to result in steady shrinkage of the cell and ultimately resulted in hemolysis. In contrast to intact senescent erythrocytes, the remnant erythrocyte ghost shells were prone to recognition and breakdown by red pulp macrophages. These data identify hemolysis as a key event in the turnover of senescent erythrocytes, which alters our current understanding of how erythrocyte degradation is regulated.

Isolation and Characterization of Human Epidermal Stem Cells

1996 ◽  
Vol 91 (2) ◽  
pp. 141-146 ◽  
Author(s):  
P. H. Jones
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Matrix Proteins ◽  
Stem Cell Markers ◽  
Epidermal Stem Cells ◽  
Cell Behaviour ◽  
Isolation And Characterization ◽  
Integrin Family

1. The keratinocytes in human epidermis are constantly turned over and replaced by a population of stem cells located in the basal epidermal layer. Until recently there were no markers allowing the isolation of viable epidermal stem cells. However, it has now been shown that epidermal stem cells can be isolated both in vitro and direct from the epidermis as they express high levels of functional β1 integrin family receptors for extracellular matrix proteins. 2. The evidence for integrins as stem cell markers and the insights that have been gained into stem cell behaviour are reviewed.

scholarly journals Evolution of Nacre: Biochemistry and Proteomics of the Shell Organic Matrix of the CephalopodNautilus macromphalus

ChemBioChem ◽  
2009 ◽  
Vol 10 (9) ◽  
pp. 1495-1506 ◽  
Author(s):  
Benjamin Marie ◽  
Frédéric Marin ◽  
Arul Marie ◽  
Laurent Bédouet ◽  
Lionel Dubost ◽  
...  
Keyword(s):  
Organic Matrix ◽  
Shell Organic Matrix

Enhancement of the Photovoltaic Potential in Mimosa Pudica-Based Dye for Sensitization of the Working Electrode in the Construction of Solar Cell

2021 ◽  
pp. 1-21
Author(s):  
Manasseh B. Shitta ◽  
Emmanuel O.B. Ogedengbe ◽  
Oluwole B. Familoni ◽  
Oluwatoyin T. Ogundipe
Keyword(s):  
Solar Cell ◽  
Conversion Efficiency ◽  
Organic Matrix ◽  
Tio2 Layer ◽  
Mimosa Pudica ◽  
Solar Module ◽  
Renewable Power ◽  
Diffusion Characteristics ◽  
In Series

Abstract The potential enhancement of extract from Mimosa pudica (M.pudica) leaf for sensitizing TiO2 layer towards the production of organic solar cell is investigated. A unique diffusion model that incorporates the concentration of the extract in the TiO2 layer is adopted. The diffusion characterization of the extract into the TiO2 provides a proper understanding of the dynamics of the extract within the layer. This research applies the combination of experimental and numerical techniques towards the investigation of the diffusion characteristics in Mimosa pudica extract. Experimental chromatograph of the extract is conducted in order to reveal the properties and concentration of the extract. Three different thickness of TiO2 deposit, and are sensitized at different hours in order to monitor the absorbance. Using the finite volume method (FVM), the adsorption and diffusion characteristics of the extract into the layer of TiO2 are modelled. The current voltage characteristics of the cell are combined in series as a standard module and its application modelled in an audited office space. The cell area characterised is 0.3848 cm2, the conversion efficiency of 1.35 % is obtained. The concentration model of the extract in TiO2 and the entrance velocity is presented. The experimental and numerical results compared favourably. However, it is anticipated that additional taxonomical characterization of M.pudica and advanced investigation into organic matrix composite will provide a useful guide for the synthesis of the natural dye and enhance the conversion efficiency of the solar module for renewable power generation.

Organic matrix synthesis in the scleractinian coral stylophora pistillata: role in biomineralization and potential target of the organotin tributyltin

1998 ◽  
Vol 201 (13) ◽  
pp. 2001-2009 ◽  
Author(s):  
D Allemand ◽  
É Tambutté ◽  
JP Girard ◽  
J Jaubert
Keyword(s):  
Protein Synthesis ◽  
Aspartic Acid ◽  
Scleractinian Coral ◽  
Organic Matrix ◽  
Coral Skeleton ◽  
Matrix Proteins ◽  
Coral Tissue ◽  
Lag Phase ◽  
Acid Uptake ◽  
Stylophora Pistillata

The kinetics of organic matrix biosynthesis and incorporation into scleractinian coral skeleton was studied using microcolonies of Stylophora pistillata. [14C]Aspartic acid was used to label the organic matrix since this acidic amino acid can represent up to 50 mol % of organic matrix proteins. External aspartate was rapidly incorporated into tissue protein without any detectable lag phase, suggesting either a small intracellular pool of aspartic acid or a pool with a fast turn-over rate. The incorporation of 14C-labelled macromolecules into the skeleton was linear over time, after an initial delay of 20 min. Rates of calcification, measured by the incorporation of 45Ca into the skeleton, and of organic matrix biosynthesis and incorporation into the skeleton were constant. Inhibition of calcification by the Ca2+ channel inhibitor verapamil reduced the incorporation of organic matrix proteins into the skeleton. Similarly, organic matrix incorporation into the skeleton, but not protein synthesis for incorporation into the tissue compartment, was dependent on the state of polymerization of both actin and tubulin, as shown by the sensitivity of this process to cytochalasin B and colchicin. These drugs may inhibit exocytosis of organic matrix proteins into the subcalicoblastic space. Finally, inhibition of protein synthesis by emetin or cycloheximide and inhibition of N-glycosylation by tunicamycin reduced both the incorporation of macromolecules into the skeleton and the rate of calcification. This suggests that organic matrix biosynthesis and its migration towards the site of calcification may be a prerequisite step in the calcification process. On the basis of these results, we investigated the effects of tributyltin (TBT), a component of antifouling painting known to interfere with biomineralization processes. Our results have shown that this xenobiotic significantly inhibits protein synthesis and the subsequent incorporation of protein into coral skeleton. This effect was correlated with a reduction in the rate of calcification. Protein synthesis was shown to be the parameter most sensitive to TBT (IC50=0.2 micromol l-1), followed by aspartic acid uptake by coral tissue (IC50=0.6 micromol l-1), skeletogenesis (IC50=3 micromol l-1) and Ca2+ uptake by coral tissue (IC50=20 micromol l-1). These results suggest that the mode of action of TBT on calcification may be the inhibition of organic matrix biosynthesis.

scholarly journals Synthesis and Characterization of Rutile Pigments with Cr and Nb

2014 ◽  
Vol 2014 ◽  
pp. 1-8
Author(s):  
Jan Večeřa ◽  
Iveta Šedová ◽  
Petr Mikulášek ◽  
Petra Šulcová
Keyword(s):  
Particle Size ◽  
Solid State ◽  
Particle Size Distribution ◽  
Solid State Reaction ◽  
Organic Matrix ◽  
Synthesis And Characterization ◽  
Visual Color ◽  
Rutile Pigments ◽  
Titanium Compounds

Rutile pigments Ti1-3xCrxNb2xO2±δ (where x=0, 0.05, 0.10, 0.20, 0.30 and 0.50) prepared by solid-state reaction are investigated. Chromium is chromophore (coloring ion) and niobium is counterion (charge-compensating element for electroneutrality). The effect of composition (x), calcination temperature (850, 900, 950, 1000, 1050, 1100 and 1150°C), and starting titanium compounds (anatase TiO2, hydrated anatase paste, TiOSO4·2H2O, and hydrated Na2Ti4O9 paste) on their color properties into organic matrix and particle size distribution was observed. According to the highest chroma C and visual color evaluation, yellow and orange pigments were selected as in color the most interesting. They have concentration x=0.05 or 0.10 and are prepared from anatase TiO2 and TiOSO4·2H2O at temperature ≥1050°C.

Sign in / Sign up

Export Citation Format

Share Document