Development of fully automatable multidimensional liquid chromatography (MDLC) with online tandem mass spectrometry for shotgun proteomics

2011 ◽  
Author(s):  
Pak-wing Kong
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Shotgun Proteomics ◽  
Tandem Mass

scholarly journals Simultaneous Identification of Multiple β-Lactamases in Acinetobacter baumannii in Relation to Carbapenem and Ceftazidime Resistance, Using Liquid Chromatography-Tandem Mass Spectrometry: TABLE 1

2015 ◽  
Vol 53 (6) ◽  
pp. 1927-1930 ◽  
Author(s):  
Hein Trip ◽  
Katrin Mende ◽  
Joanna A. Majchrzykiewicz-Koehorst ◽  
Norbert J. A. Sedee ◽  
Albert G. Hulst ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Acinetobacter Baumannii ◽  
Shotgun Proteomics ◽  
Added Value ◽  
Tandem Mass ◽  
Content Type ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass

Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinicalAcinetobacter baumanniiisolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing.

Tandem Mass Spectrometry in Physiology

Physiology ◽  
2007 ◽  
Vol 22 (6) ◽  
pp. 390-400 ◽  
Author(s):  
Trairak Pisitkun ◽  
Jason D. Hoffert ◽  
Ming-Jiun Yu ◽  
Mark A. Knepper
Keyword(s):  
Mass Spectrometry ◽  
Liquid Chromatography ◽  
Tandem Mass Spectrometry ◽  
Recent Progress ◽  
Multiple Reaction Monitoring ◽  
Complex Mixture ◽  
Shotgun Proteomics ◽  
Cell Physiology ◽  
Tandem Mass ◽  
Reaction Monitoring

Tandem mass spectrometry coupled to liquid chromatography (LC-MS/MS) allows identification of proteins in a complex mixture without need for protein purification (“shotgun” proteomics). Recent progress in LC-MS/MS-based quantification, phosphoproteomic analysis, and targeted LC-MS/MS using multiple reaction monitoring (MRM) has made LC-MS/MS a powerful tool for the study of cell physiology.

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