Phenotypic and molecular characterization of a blood culture isolate of the family coriobacteriaceae

2009 ◽  
Author(s):  
Kit-mei Lam
Keyword(s):  
Blood Culture ◽  
Molecular Characterization ◽  
Blood Culture Isolate ◽  
The Family ◽  
Culture Isolate

scholarly journals Molecular Characterization of a Catalase-Negative Staphylococcus aureus Blood Culture Isolate: TABLE 1

2015 ◽  
Vol 53 (11) ◽  
pp. 3699-3701 ◽  
Author(s):  
Jeanette W. P. Teo ◽  
Steven Kum ◽  
Roland Jureen ◽  
Raymond T. P. Lin
Keyword(s):  
Amino Acids ◽  
Staphylococcus Aureus ◽  
Blood Culture ◽  
Molecular Characterization ◽  
Frameshift Mutation ◽  
Gene Encoding ◽  
Blood Culture Isolate ◽  
Content Type ◽  
Culture Isolate

Here we report a catalase-negative methicillin-sensitiveStaphylococcus aureusisolate collected from a blood culture. Sequencing through the gene encoding catalase,katA, demonstrated a 2-bp insertion. The resulting frameshift mutation generates a protein that has lost 26 amino acids (aa) at its C-terminal domain.

scholarly journals Molecular characterization of a novel mycovirus of the family Tymoviridae isolated from the plant pathogenic fungus Fusarium graminearum

Virology ◽  
2016 ◽  
Vol 489 ◽  
pp. 86-94 ◽  
Author(s):  
Pengfei Li ◽  
Yanhong Lin ◽  
Hailong Zhang ◽  
Shuangchao Wang ◽  
Dewen Qiu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Fusarium Graminearum ◽  
Pathogenic Fungus ◽  
Plant Pathogenic Fungus ◽  
The Family

scholarly journals Molecular characterization of the largest mycoviral-like double-stranded RNAs associated with Amasya cherry disease, a disease of presumed fungal aetiology

2006 ◽  
Vol 87 (10) ◽  
pp. 3113-3117 ◽  
Author(s):  
Z. Kozlakidis ◽  
L. Covelli ◽  
F. Di Serio ◽  
A. Citir ◽  
S. Açıkgöz ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Unknown Function ◽  
Functional Relationship ◽  
Rna Polymerases ◽  
Partial Characterization ◽  
The Family

The sequence of the four large (L) double-stranded RNAs (dsRNAs) associated with Amasya cherry disease (ACD), which has a presumed fungal aetiology, is reported. ACD L dsRNAs 1 (5121 bp) and 2 (5047 bp) potentially encode proteins of 1628 and 1620 aa, respectively, that are 37 % identical and of unknown function. ACD L dsRNAs 3 (4458 bp) and 4 (4303 bp) potentially encode proteins that are 68 % identical and contain the eight motifs conserved in RNA-dependent RNA polymerases (RdRp) of dsRNA mycoviruses, having highest similarity with those of members of the family Totiviridae. Both terminal regions share extensive conservation in all four RNAs, suggesting a functional relationship between them. As ACD L dsRNAs 1 and 2 do not encode RdRps, both are probably replicated by those from either ACD L dsRNA 3 or 4. Partial characterization of the equivalent L dsRNAs 3 and 4 associated with cherry chlorotic rusty spot revealed essentially identical sequences.

Molecular characterization of a virus from the family Luteoviridae associated with cotton blue disease

2005 ◽  
Vol 150 (7) ◽  
pp. 1357-1367 ◽  
Author(s):  
R. L. Corrêa ◽  
T. F. Silva ◽  
J. L. Simões-Araújo ◽  
P. A. V. Barroso ◽  
M. S. Vidal ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
The Family ◽  
Cotton Blue ◽  
Cotton Blue Disease

scholarly journals Nasopharyngeal colonization at birth and the development of early-onset neonatal sepsis

2020 ◽  
Vol 60 (6) ◽  
pp. 287-92
Author(s):  
Andi Dwi Bahagia Febriani ◽  
Nilam Sartika Putri ◽  
Ema Alasiry ◽  
Dasril Daud
Keyword(s):  
Blood Culture ◽  
Neonatal Sepsis ◽  
Early Onset ◽  
Bacterial Colonization ◽  
Bacterial Species ◽  
Nasopharyngeal Swab ◽  
Nasopharyngeal Colonization ◽  
Blood Culture Isolate ◽  
Significant Difference ◽  
Culture Isolate

Background Neonatal sepsis is one of the major causes of morbidity and mortality in neonates. Exposure to maternal bacteria during pregnancy or delivery allows for colonization of the normal upper airway. Such bacteria become the major ecological species in the infant. If the colonizing bacteria invade the bloodstream, early-onset neonatal sepsis (EONS) could occur. Objective To evaluate for an association between colonization of the newborn nasopharynx and EONS, as well as for agreement between nasopharyngeal swab culture and blood culture isolate results. Methods This prospective cohort study was conducted in Dr. Wahidin Sudirohusodo General Hospital and Ibnu Sina Hospital, Makassar, South Sulawesi. Nasopharyngeal swab culture was taken within 2 hours of life from newborns who met the inclusion criteria, then they were followed up for signs of EONS. Blood culture was taken from subject with EONS. Results Of the 100 newborns, 69 (69%) had nasopharyngeal bacterial colonization, of whom 5.8% (4/69) experienced EONS. Of the remaining 31 (31%) without colonization, 9.7% (3/31) experienced EONS. There was no significant difference in frequency of EONS between newborns with and without nasopharyngeal colonization. Although Gram-negative bacteria were predominant among colonized newborns, there was no significant difference to numbers of Gram-positive bacteria as a causative agent of EONS. Only one patient with EONS had the same bacterial species in both the nasopharynx and blood culture isolate. Conclusion  Newborn nasopharyngeal colonization at birth is not associated with EONS.

Evaluation of Skin Antisepsis Prior to Blood Culture in Neonates

1984 ◽  
Vol 5 (10) ◽  
pp. 489-491 ◽  
Author(s):  
Sylvie Champagne ◽  
Susan Fussell ◽  
David Scheifele
Keyword(s):  
Blood Culture ◽  
Staphylococcus Epidermidis ◽  
Coagulase Negative Staphylococcus ◽  
Two Phase ◽  
Blood Culture Isolate ◽  
Forearm Skin ◽  
Skin Antisepsis ◽  
Intensive Care Nursery ◽  
Culture Isolate ◽  
Residual Growth

AbstractIn our Intensive Care Nursery, coagulase-negative staphylococcus is the most frequent blood culture isolate. As skin antisepsis is critical in preventing blood culture contamination, we examined the efficacy of the chlorhexidine tincture (CH) used in our nursery for this purpose. Staphylococcus epidermidis colonized the forearms of 88% of infants tested, in a mean density of 104 organisms/cm2. Following a 60-second application of CH (0.5% in 70% ethanol), bacterial growth from forearm skin remained abundant in 15/38 infants (39.4%). Cleansing with 70% isopropyl alcohol, followed by CH as above, left abundant residual growth in only 1/37 infants (2.7%) (P<0.001). All 136 S. epidermidis tested were susceptible to CH (MIC<5(μg/ml) and 14 of 15 exposed to CH 0.02% were rapidly killed (≥98% fall in viable counts within 90 sec). We conclude that two-phase antisepsis using isopropanol followed by CH is a more effective preparation for blood culture in neonates than is CH alone.

Carbapenem Resistance via the blaKPC-2 Gene in Enterobacter cloacae Blood Culture Isolate

2010 ◽  
Vol 103 (5) ◽  
pp. 453-454 ◽  
Author(s):  
Andy Lo ◽  
Rosemary Verrall ◽  
John Williams ◽  
Charles Stratton ◽  
Phyllis Della-Latta ◽  
...  
Keyword(s):  
Blood Culture ◽  
Carbapenem Resistance ◽  
Enterobacter Cloacae ◽  
Blood Culture Isolate ◽  
Culture Isolate
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