Proteomics study of the effects of fish oil and corn oil enriched diet on membranous nephritis

2008 ◽  
Author(s):  
Yisha Ye
Keyword(s):  
Fish Oil ◽  
Corn Oil ◽  
Proteomics Study ◽  
Membranous Nephritis

Effects of high fat fish oil and high fat corn oil diets on initiation of AOM-induced colonic aberrant crypt foci in male F344 rats

2003 ◽  
Vol 41 (12) ◽  
pp. 1739-1747 ◽  
Author(s):  
Yvonne E.M. Dommels ◽  
Suzanne Heemskerk ◽  
Hans van den Berg ◽  
Gerrit M. Alink ◽  
Peter J. van Bladeren ◽  
...  
Keyword(s):  
Fish Oil ◽  
Corn Oil ◽  
Aberrant Crypt Foci ◽  
High Fat ◽  
F344 Rats ◽  
Male F344 Rats

scholarly journals Tumor Growth Dynamics: Dietary Fish Oil Induced Inhibition of Human Breast Carcinoma Growth, A Phenomenon of Reduced Cellular DNA Synthesis or Increased Cell Loss?

2017 ◽  
Vol 1 (1) ◽  
pp. 1-13
Author(s):  
Michael J Gonzalez ◽  
Jorge Duconge
Keyword(s):  
Cell Proliferation ◽  
Tumor Cell ◽  
Fish Oil ◽  
Corn Oil ◽  
Carcinoma Cell ◽  
Flow Cytometric Analysis ◽  
Cell Loss ◽  
Tumor Cell Proliferation ◽  
Menhaden Oil ◽  
Human Breast

Diets high in unsaturated fatty acids, especially those containing high levels of linoleic acid, e.g., corn oil, enhance mammary gland tumorigenesis in experimental animals. In contrast, diets high in long-chain polyunsaturated fatty acids such as eicosapentaenoic (EPA) and docosahexaenoic (DHA), e.g. menhaden oil, appear to have a suppressive effect on this tumorigenic process. Many mechanisms have been proposed to explain the tumor inhibitory action exerted by menhaden oil and other fish oils, e.g., differences in prostaglandin metabolism, energy efficiency, alterations of the immune system, changes in lipid peroxidation, etc. Fundamental to a mechanistic understanding of this phenomenon, however, is an understanding as to whether or not the tumor inhibitory activities of dietary fish oil is mediated via an inhibition of tumor cell proliferation or mediated via an enhancement of tumor cell loss. Whether the amount of dietary fat or the type of fat effects mammary tumorigenic processes, via an effect on tumor cell proliferation or tumor cell loss, has not been clearly established. In the studies described in this communication, three methods were utilized to study tumor cell proliferation, i.e., H3-thymidine autoradiographic analysis, 5-bromo 2'-deoxyuridine (Brdu) flow cytometric analysis, and proliferative cell nuclear antigen (PCNA) flow cytometric analysis. Two methods were used to study tumor cell loss, i.e., a determination of the I125Urd tumor emission rate and a determination of a cell loss factor from the formulas of Steel and Begg. The tumor examined was the human breast carcinoma cell line MDA- MB231 maintained in athymic nude mouse. No significant difference in cell proliferation between carcinomas of mice fed a high corn oil diet (20% w/w) and a diet high in fish oil (19% menhaden oil, 1% corn oil). In contrast, a significant (p<0.05) increase in the rate of I125Urd emission rate and cell loss factor from the carcinomas in the fish oil fed mice compared to the corn oil fed mice was observed. In summary, the decreased tumor volume in the human breast carcinomas maintained in athymic nude mice fed a fish oil diet as compared to those fed a corn oil diet, appears to be due, at least in part, to an increased rate of carcinoma cell loss rather than a decreased rate of carcinoma cell proliferation.

scholarly journals The Effect of Enriched Daphnia sp. with Different Source of Oil on the Survival Rate and the Growth of Oreochromis niloticus Larvae

2007 ◽  
Vol 2 (1) ◽  
pp. 7 ◽  
Author(s):  
I. Mokoginta ◽  
Dedi Jusadi ◽  
T.L. Pelawi
Keyword(s):  
Fatty Acid ◽  
Survival Rate ◽  
Fish Oil ◽  
Oreochromis Niloticus ◽  
Acid Level ◽  
Corn Oil ◽  
Lipid Level ◽  
Font Size ◽  
Fatty Acid Level ◽  
Ad Libitum

<p class="MsoNormal" style="text-align: justify;"><span style="font-size: 11pt;" lang="in" xml:lang="in">ABSTRACT<br /></span></p><p class="MsoNormal" style="text-align: justify;"><span style="font-size: 11pt;" lang="in" xml:lang="in">This experiment was conducted to evaluate best source of oil to enriched <em>Daphnia</em> sp. before fed it to <em>Oreochromis niloticus</em> larvae. Four treatments were used in this experiment; first, <em>Daphnia</em> sp. without encrichment, second <em>Daphnia</em> sp. enriched with fish oil, third <em>Daphnia</em> sp. enriched with corn oil and the fourth <em>Daphnia</em> sp. enriched with coconut oil. Three days old larvae fed on <em>Daphnia</em> sp. with size of </span><span style="font-size: 11pt; font-family: Symbol;" lang="in" xml:lang="in"><span>£</span></span><span style="font-size: 11pt;" lang="in" xml:lang="in"> 0,5 mm at the first week and 0,6 – 1,0 mm as the second week of this experiment. Larvae fed on <em>Daphnia</em> sp. 5 times daily, <em>ad libitum</em>, for 14 days. Larvae was reared in the small cages (2,25 l), and all cages was placed in the aquarium. Larvae density was 48 larvae/l. This experiment showed that the lipid level in <em>Daphnia</em> sp. enriched with oil was higher than that of no enrichment <em>Daphnia</em> sp. The highest n3- fatty acid level was found in <em>Daphnia</em> sp. enriched with fish oil, and the highest n6- fatty acid level was found in <em>Daphnia</em> sp. enriched with corn oil. Larvae fed on <em>Daphnia</em> sp. enriched with oil have a higher relative growth rate than that fed on <em>Daphnia</em> sp. without enrichment. The highest survival rate of larvae was found by feeding them with <em>Daphnia</em> sp. enriched with corn oil (<em>p</em> &lt; 0,05).</span></p><p class="MsoNormal" style="text-align: justify;"><span style="font-size: 11pt;" lang="in" xml:lang="in">Key words : <em>Daphnia</em> sp., enrichment, larvae, <em>Oreochromis niloticus</em></span></p><p class="MsoNormal"><span style="font-size: 11pt;"> </span></p><h2 style="margin-top: 0cm; text-align: center;"><span style="font-size: 11pt;" lang="in" xml:lang="in">ABSTRAK</span></h2><p class="MsoNormal" style="text-align: justify;"><span style="font-size: 11pt;" lang="in" xml:lang="in">Penelitian ini dilakukan untuk mengevaluasi sumber minyak yang terbaik bagi pengkayaan <em>Daphnia</em> sp. sebelum diberikan ke larva ikan nila, <em>Oreochromis niloticus</em>. Ada 4 perlakuan dalam penelitian ini yaitu <em>Daphnia</em> sp. yang tidak diperkaya minyak; <em>Daphnia</em> sp. yang diperkaya minyak ikan; <em>Daphnia</em> sp. yang diperkaya minyak jagung; dan <em>Daphnia</em> sp. yang diperkaya minyak kelapa. Larva ikan nila, <em>Oreochromis niloticus </em>berumur 3 hari ditebar dalam hapa kecil (volume 2,25 l) sebanyak 48 ekor/l, dan seluruh hapa diletakkan dalam akuarium berukuran 100x50x40 cm. <em>Daphnia</em> sp. diperkaya terlebih dahulu dengan minyak sesuai perlakuan sebelum diberikan ke larva. Pada minggu pertama pemeliharaan larva, ukuran <em>Daphnia</em> sp. yang digunakan adalah </span><span style="font-size: 11pt; font-family: Symbol;" lang="in" xml:lang="in"><span>£</span></span><span style="font-size: 11pt;" lang="in" xml:lang="in"> 0,5 mm dan pada minggu ke dua 0.6 – 1.0 mm. <em>Daphnia</em> sp. diberikan sebanyak 5 kali dalam sehari secara <em>ad libitum</em> dan pemberian pakan dilakukan selama 14 hari. Hasil penelitian ini memperlihatkan bahwa kadar lemak <em>Daphnia</em> sp. yang diperkaya minyak lebih tinggi dari <em>Daphnia</em> sp. yang tidak diperkaya, dan hal ini berpengaruh pula pada kadar lemak tubuh larva. Kadar asam lemak –n3 tertinggi terdapat pada <em>Daphnia</em> sp. yang diperkaya minyak ikan dan kadar asam lemak –n6 tertinggi terdapat pada <em>Daphnia</em> sp. yang diperkaya minyak jagung. Larva yang diberi <em>Daphnia</em> sp. yang diperkaya dengan minyak mempunyai pertambahan bobot relatif dan tingkat kelangsungan hidup yang lebih tinggi dari larva yang diberi <em>Daphnia</em> sp. yang tidak diperkaya; dan tingkat kelangsungan hidup larva yang tertinggi dihasilkan oleh perlakuan pemberian <em>Daphnia</em> sp. yang diperkaya minyak jagung (P &lt; 0,05), walaupun pertumbuhan bobot relatifnya sama dengan perlakuan lainnya (<em>p</em> &gt; 0,05).</span></p><p class="MsoNormal" style="text-align: justify;"><span style="font-size: 11pt;" lang="in" xml:lang="in">Kata kunci : <em>Daphnia</em> sp., pengkayaan, larva, <em>Oreochromis niloticus</em></span></p>

Effect of fatty acid composition of dietary fat on energy balance and expenditure in hamsters

1989 ◽  
Vol 67 (9) ◽  
pp. 994-998 ◽  
Author(s):  
Peter J. H. Jones
Keyword(s):  
Oxygen Consumption ◽  
Energy Balance ◽  
Energy Expenditure ◽  
Fish Oil ◽  
Corn Oil ◽  
Dietary Fatty Acids ◽  
Metabolizable Energy ◽  
Before And After ◽  
Metabolizable Energy Intake ◽  
Energy Gains

The comparative effects of feeding diets containing corn, olive, coconut, or menhaden fish oil on efficiency of energy deposition and on short term energy expenditure were examined in growing hamsters. Diets comprising oils mixed with laboratory diets at 10% oil w/w were fed ad libitum for 3 weeks. Animals fed laboratory diets were used as controls. Body composition was determined before and after the feeding period using 3H2O distribution space. Oxygen consumption was measured in each animal during the final week. Weight gains of groups fed corn and olive oil diets exceeded those of the group fed laboratory diet alone (p < 0.05), although metabolizable energy intakes were similar across groups. Corn oil fed animals demonstrated higher carcass energy gains as fat compared with laboratory diet fed or menhaden oil fed groups. This was reflected in an increased fractional deposition of metabolizable energy intake in the group fed corn oil diet compared with the latter two groups. Fecal energy losses were lower in the group fed corn oil diet, and higher in the group fed laboratory diet alone, compared with other groups. Oxygen consumption did not differ between groups. These findings indicate that feeding dietary fish oil, compared with corn oil, favours energy substrate oxidation reducing the fraction of metabolizable energy partitioned for storage.Key words: energy balance, energy expenditure, dietary fatty acids, hamster.

Inhibition of DNA replication by fish oil-treated cytoplasm is counteracted by fish oil-treated nuclear extract

2002 ◽  
Vol 283 (5) ◽  
pp. C1365-C1375 ◽  
Author(s):  
Sybille Rex ◽  
Maria A. Kukuruzinska ◽  
Nawfal W. Istfan
Keyword(s):  
Fatty Acids ◽  
Dna Replication ◽  
Dna Synthesis ◽  
Fish Oil ◽  
Corn Oil ◽  
Nuclear Extract ◽  
Inhibitory Effect ◽  
Free System ◽  
Nuclear Factors ◽  
Cytosolic Extract

We have recently noted that cells treated with fish oil and n–3-fatty acids show slower DNA replication rates than cells treated with a control emulsion or corn oil only. However, it is not clearly understood how such an effect is induced. Fish oil and its metabolites are known to have several modulating effects on signal transduction pathways. Alternatively, they may influence DNA replication by interacting directly with nuclear components. To investigate this problem in greater detail, we have studied the kinetics of DNA synthesis in a cell-free system derived from HeLa cells. Nuclei and cytosolic extract were isolated from cells synchronized in early S phase after treatment with control emulsion, corn oil, or fish oil, respectively. The nuclei were reconstituted with cytosolic extract and a reaction mixture containing bromodeoxyuridine (BrdU) triphosphate to label newly synthesized DNA. The rate of DNA synthesis was measured by bivariate DNA/BrdU analysis and flow cytometry. We show that fish oil-treated cytosol inhibits the elongation of newly synthesized DNA by ∼80% in control nuclei. However, nuclei treated with fish oil escape this inhibitory effect. We also show that addition of nuclear extract from fish oil-treated cells reverses the inhibitory effect seen in the reconstitution system of control nuclei and fish oil-treated cytosol. These results indicate that polyunsaturated fatty acids can modulate DNA synthesis through cytosolic as well as soluble nuclear factors.

scholarly journals Dietary fish oil supplementation alters liver gene expressions to protect against LPS-induced liver injury in weanling piglets

2019 ◽  
Vol 25 (1) ◽  
pp. 60-72 ◽  
Author(s):  
Jing Zhang ◽  
Xin Xu ◽  
Huiling Zhu ◽  
Yang Wang ◽  
Yongqing Hou ◽  
...  
Keyword(s):  
Liver Injury ◽  
Fish Oil ◽  
Rna Sequencing ◽  
Corn Oil ◽  
Sequencing Analysis ◽  
Protective Effects ◽  
Gene Expressions ◽  
Inflammatory Genes ◽  
Lps Challenge ◽  
Fish Oil Supplementation

Here, the potential mechanisms of the protective effects of fish oil against LPS-induced liver injury in a piglet model were investigated by using RNA sequencing. Twenty-four piglets were used in a 2 × 2 factorial design, and the main factors included diet (5% corn oil or 5% fish oil) and immunological challenge (LPS or saline, on d 19). All piglets were slaughtered at 4 h after challenge, and liver samples were collected. Fish oil improved liver morphology and reduced TNF-α, IL-1β and IL-6 productions after LPS challenge. RNA sequencing analysis showed fish oil had significant effect on the expressions of genes involved in immune response during LPS-induced inflammation. Selected gene expression changes were validated using quantitative RT-PCR. Fish oil reduced the expressions of pro-inflammatory genes IL1R1, IL1RAP, CEBPB and CRP, and increased that of anti-inflammatory genes IL-18BP, NFKBIA, IFIT1, IFIT2 and ATF3. Moreover, fish oil restored the expressions of some lipid metabolism-related genes, such as ACAA1, ACACA, ACADS and ACADM, which were only decreased in pigs fed a corn oil diet after LPS challenge. Our RNA sequencing reveals novel gene-nutrient interactions following fish oil supplementation and evoked inflammation, which add to the current understanding of the benefits of n-3 polyunsaturated fatty acids against liver injury.

scholarly journals Dietary fish oil modulates macrophage fatty acids and decreases arthritis susceptibility in mice.

1985 ◽  
Vol 162 (4) ◽  
pp. 1336-1349 ◽  
Author(s):  
C A Leslie ◽  
W A Gonnerman ◽  
M D Ullman ◽  
K C Hayes ◽  
C Franzblau ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Fish Oil ◽  
Corn Oil ◽  
Type Ii Collagen ◽  
Chow Diet ◽  
Standard Diet ◽  
Type Ii ◽  
Fish Oil Diet

B10.RIII and B10.G mice were transferred from a diet of laboratory rodent chow to a standard diet in which all the fat (5% by weight) was supplied as either fish oil (17% eicosapentaenoic acid [EPA], 12% docosahexaenoic acid [DHA], 0% arachidonic acid [AA], and 2% linoleic acid) or corn oil (0% EPA, 0% DHA, 0% AA, and 65% linoleic acid). The fatty acid composition of the macrophage phospholipids from mice on the chow diet was similar to that of mice on a corn oil diet. Mice fed the fish oil diet for only 1 wk showed substantial increases in macrophage phospholipid levels of the omega-3 fatty acids (of total fatty acid 4% was EPA, 10% docosapentaenoic acid [DPA], and 10% DHA), and decreases in omega-6 fatty acids (12% was AA, 2% docosatetraenoic acid [DTA], and 4% linoleic acid) compared to corn oil-fed mice (0% EPA, 0% DPA, 6% DHA, 20% AA, 9% DTA, and 8% linoleic acid). After 5 wk this difference between the fish oil-fed and corn oil-fed mice was even more pronounced. Further small changes occurred at 5-9 wk. We studied the prostaglandin (PG) and thromboxane (TX) profile of macrophages prepared from mice fed the two diets just before being immunized with collagen. Irrespective of diet, macrophages prepared from female mice and incubated for 24 h had significantly more PG and TX in the medium than similarly prepared macrophages from male mice. The increased percentage of EPA and decreased percentage of AA in the phospholipids of the macrophages prepared from the fish oil-fed mice was reflected in a reduction in the amount of PGE2 and PGI2 in the medium relative to identically incubated macrophages prepared from corn oil-fed mice. When this same fish oil diet was fed to B10.RIII mice for 26 d before immunization with type II collagen, the time of onset of arthritis was increased, and the incidence and severity of arthritis was reduced compared to arthritis induced in corn oil-fed mice. The females, especially those on the fish oil diet, tended to have less arthritis than the males. These alterations in the fatty acid pool available for PG and leukotriene synthesis suggest a pivotal role for the macrophage and PG in the immune and/or inflammatory response to type II collagen.

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