Comparative study on molecular and serological diagnosis of tuberculosis

Serological diagnosis ofErysipelothrix rhusiopathiae:A comparative study between the Growth Inhibition Test and the Complement Fixation Test

Veterinary Quarterly ◽

10.1080/01652176.1981.9693789 ◽

1981 ◽

Vol 3 (1) ◽

pp. 19-24

Author(s):

Z. Bercovich ◽

C.D. Weenink van Loon ◽

C.W. Spel

Keyword(s):

Comparative Study ◽

Growth Inhibition ◽

Complement Fixation Test ◽

Complement Fixation ◽

Inhibition Test ◽

Fixation Test ◽

Serological Diagnosis ◽

Growth Inhibition Test

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Serological diagnosis of bovine neosporosis: A comparative study of commercially available ELISA tests

Veterinary Parasitology ◽

10.1016/j.vetpar.2013.07.033 ◽

2013 ◽

Vol 198 (1-2) ◽

pp. 85-95 ◽

Cited By ~ 31

Author(s):

Gema Alvarez-García ◽

Alicia García-Culebras ◽

Daniel Gutiérrez-Expósito ◽

Vanesa Navarro-Lozano ◽

Iván Pastor-Fernández ◽

...

Keyword(s):

Comparative Study ◽

Serological Diagnosis ◽

Bovine Neosporosis

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A comparative study of serological diagnosis of Dengue outbreak 2019

African Health Sciences ◽

10.4314/ahs.v21i3.20 ◽

2021 ◽

Vol 21 (3) ◽

pp. 1117-1123

Author(s):

Muhammad Bilal Habib ◽

Noreen Sher Akbar ◽

Anber Saleem

Keyword(s):

Dengue Virus ◽

Laboratory Test ◽

Comparative Study ◽

Blood Test ◽

Serological Diagnosis ◽

Blood Picture ◽

Dengue Outbreak ◽

Negative Results ◽

Remarkable Reduction ◽

Serology Testing

Purpose: The interpretation & correlation of the different laboratory parameters in positive dengue cases in order to eval- uate that which laboratory test is more significant for diagnosis of Dengue. Methods: Prospective examination of samples (patients’ serum) for dengue virus of different genotype by using multiplex anti-dengue IgM, IgG. We have done NS-1 test by (ICT) immunochromatographic devices, and complete blood picture (CBC) by Sysmex XP-100. Result: Detection of Viral RNA in 100 patients showed effects in the total of 73 (73.0%) samples. This graphical compar- ison shows the whole positive cases including dengue NS-I antigen, dengue serology (IgM & IgG), total 62 positive cases of NS-I are detected, 10 positive cases of dengue IgM and 9 positive cases of IgG detected, in which Complete Blood Test (CBC) shows remarkable reduction in Platelets (32 cases) and Leucopenia in (24 positive cases). Conclusion: In this research, it is concluded that the diagnosis of dengue cases is preliminary limited to initial stages i.e. CBC or sometimes dengue NS-I, as dengue IgM severity is more effective than that of Dengue NS-I & IgG. Many patients who had negative results in CBC and NS-1 testing, became positive when IgM and IgG serology testing has been done. Keywords: Dengue; NS-1; IgG; IgM; Immunochrometographic test.

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A PROCEDURE FOR THE SEROLOGICAL DIAGNOSIS OF SYPHILIS BASED ON A COMPARATIVE STUDY OF FIVE MODERN SEROLOGICAL TESTS

Sexually Transmitted Infections ◽

10.1136/sti.20.3.118 ◽

1944 ◽

Vol 20 (3) ◽

pp. 118-123

Author(s):

F. M. Berger ◽

G. Denton

Keyword(s):

Comparative Study ◽

Serological Diagnosis ◽

Serological Tests

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Comparative Study of Indirect Fluorescent Antibody, ELISA, and Immunochromatography Tests for Serological Diagnosis of Bovine Babesiosis Caused by Babesia bovis

Animals ◽

10.3390/ani11123358 ◽

2021 ◽

Vol 11 (12) ◽

pp. 3358

Author(s):

José Juan Lira-Amaya ◽

Grecia Martínez-García ◽

R. Montserrat Santamaria-Espinosa ◽

Roberto O. Castañeda-Arriola ◽

Juan J. Ojeda-Carrasco ◽

...

Keyword(s):

Comparative Study ◽

Sensitivity And Specificity ◽

Test Performance ◽

Fluorescent Antibody ◽

Serological Diagnosis ◽

Babesia Bovis ◽

Enzyme Linked Immunosorbent Assay ◽

Indirect Fluorescent Antibody ◽

Bovine Babesiosis ◽

Laboratory Equipment

The indirect fluorescent antibody test (IFAT) is the most frequently used test to conduct seroepidemiological studies so far, and it is regarded as the "gold standard" test for the serological diagnosis of bovine babesiosis. The aim of the present study was to compare the enzyme-linked immunosorbent assay (ELISA) and the rapid immunochromatography test (ICT) for use in the serological diagnosis of cattle exposed to B. bovis in Mexico. The evaluation of test performance was carried out with 30 positive and 30 negative reference sera. A total of 72 bovine sera samples collected from cattle in a region with endemic bovine babesiosis were analyzed by ELISA and ICT, and the results were compared with those of IFAT. Kappa value (k) was also calculated to determine the agreement between tests. The sensitivity and specificity of ELISA for detecting antibodies against B. bovis were 87% (26/30) and 80% (24/30), respectively. The sensitivity and specificity of ICT for detecting antibodies against B. bovis were 90% (27/30) and 83.3% (25/30), respectively. The overall concordance determined for ELISA and ICT was 94.4% (68/72) and 98.6% (71/72), respectively, when the results were compared with those of IFAT. ICT was more sensitive and specific in this comparative study, showing good strength of agreement (k = 0.79) with respect to IFAT. ICT combines a strip-based assay system that is fast, practical, and sensitive for detection of antibodies to B. bovis, which suggests that it could be applied in the field without requiring any laboratory equipment for its use and interpretation of test results.

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