A high yield and productivity strategy for eicosapentaenoic acid production by the diatom Nitzschia laevis in heterotrophic culture

2001 ◽  
Author(s):  
Zhiyou Wen
Keyword(s):  
Eicosapentaenoic Acid ◽  
Acid Production ◽  
High Yield ◽  
Heterotrophic Culture ◽  
Nitzschia Laevis

Optimizing Eicosapentaenoic Acid Production by Grafting a Heterologous Polyketide Synthase Pathway in the Thraustochytrid Aurantiochytrium

2020 ◽  
Vol 68 (40) ◽  
pp. 11253-11260 ◽  
Author(s):  
Sen Wang ◽  
Chuanzeng Lan ◽  
Zhuojun Wang ◽  
Weijian Wan ◽  
Huidan Zhang ◽  
...  
Keyword(s):  
Eicosapentaenoic Acid ◽  
Acid Production ◽  
Polyketide Synthase

Preparation of 14C-Labeled Penicillic Acid with High Specific Activity and Yield

1977 ◽  
Vol 40 (2) ◽  
pp. 90-93 ◽  
Author(s):  
DOUGLAS L. PARK ◽  
PHILIP B. MISLIVEC ◽  
JAMES L. HEATH
Keyword(s):  
Acid Production ◽  
Specific Activity ◽  
High Specific Activity ◽  
High Yield ◽  
Liquid Media ◽  
Penicillic Acid ◽  
Stationary Culture ◽  
Radioactive Compound ◽  
Growth Substrates ◽  
Label Incorporation

14C-Labeled penicillic acid was produced by stationary culture incubation of Penicillium cyclopium (NRRL 1888) on a modified Raulin-Thom broth medium containing 14C-labeled acetate. Approximately 1.2 g of radioactive compound, with a specific activity of 23.0 μCi/mmole, was produced in 9 days in 1500 ml of the broth. Incorporation of the isotope into penicillic acid was 11. 9%. Production of the radiolabeled compound with high specific activity was achieved by correlating the monitoring of expired 14C-CO2 with production of penicillic acid during the fermentation. The effects of various growth substrates, pH, and incubation times on production of non-labeled penicillic acid also were investigated. Results show that sterile rice is an excellent substrate, that among liquid media examined, higher yields were obtained in stationary rather than in shake cultures, and that higher yields of penicillic acid were obtained at pH 3.5 or lower. Simultaneous monitoring of penicillic acid production and 14C-label incorporation is essential to detect and isolate a high yield of labeled compound with high specific activity.

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