Secreted aspartyl proteinases of Candida albicans with particular relevance to the oral cavity

1997 ◽  
Author(s):  
Tao Wu
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Aspartyl Proteinases

Surface microhardness of different restorative materials exposed to candida albicans biofilm isolated from the oral cavity of hiv-infected children

2019 ◽  
Vol 4 (1) ◽  
pp. 87-91
Author(s):  
Leandro COSTA ◽  
Larissa SOARES-SILVA ◽  
Paulini Malfei De C. COSTA ◽  
Adrielle MANGABEIRA ◽  
Maristela PORTELA ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Surface Microhardness ◽  
Restorative Materials

scholarly journals Molecular characterization of Candida dubliniensis and Candida albicans in the oral cavity of drug abusers using duplex polymerase chain reaction

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Parastoo Hassani Abharian ◽  
Parvin Dehghan ◽  
Peyman Hassani Abharian ◽  
Sepideh Tolouei
Keyword(s):  
Polymerase Chain Reaction ◽  
Candida Albicans ◽  
Oral Cavity ◽  
Candida Species ◽  
Species Complex ◽  
Candida Dubliniensis ◽  
Drug Abusers ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Duplex Polymerase Chain Reaction

  Background and Purpose: Candida dubliniensis is closely related to the most pathogenic and prevalent yeast, namely C. albicans. Candida species can opportunistically overgrow in vulnerable individuals and cause a variety of diseases. The current study aimed to identify and isolate C. dubliniensis species present in the Candida albicans species complex identified in the oral cavity of drug abusers. Materials and Methods: This study was conducted on 53 strains of C. albicans species complex, isolated from the oral mucosa of drug abusers in Isfahan, Iran. DNA extraction was accomplished through boiling procedure. Duplex polymerase chain reaction (PCR) was performed to amplify ITS1-5.8S-ITS2 region using four specific primers. Fungal species were identified based on the difference in the size of the bands created in the Agarose gel. Results: Out of the 53 isolates under study, 30 (56.6%) and 14 (26.4%) samples were identified as C. albicans and C. dubliniensis, respectively. In the remaining 9 samples (17%), both types of Candida species were confirmed. Conclusion: The findings of the present study revealed the presence of a noticeable amount of C. dubliniensis in the oral cavity of drug abusers. Therefore, the probable presence of this fungus should be considered during the examination of oral infection among this group. To date, no research has directly investigated this issue in Iran.

scholarly journals The Prevalence and Occurrence of Candida albicans Among the Students of MAHSA University

2020 ◽  
Vol 2 (1) ◽  
pp. 89
Keyword(s):  
Candida Albicans ◽  
Oral Health ◽  
Oral Cavity ◽  
Health Knowledge ◽  
Study Group ◽  
Cross Sectional Survey ◽  
Cross Sectional ◽  
Unstimulated Saliva ◽  
Oral Health Practices ◽  
Self Administered Questionnaire

The study is done to identify the prevalence and occurrence of Candida albicans among the students of MAHSA University. It is also done to perform a cross-sectional survey by means of a self-administered questionnaire to assess the oral health knowledge, attitude, and practice (KAP) among the study group. Furthermore, it is done to determine the association of knowledge with attitude, oral health practices, and the prevalence of Candida albicans with the study group. The spores of Candida are a commensal, harmless form of a fungus that becomes invasive and pathogenic pseudohyphae when there is a disruption in the balance of flora. The infection that is caused by the yeast Candida albicans or even other candida species is candidiasis. Questionnaire on oral health was handed out to the students at random in MAHSA University, and upon that, samples are collected by taking their unstimulated saliva and are then pipetted on Sabouraud’s dextrose agar. These agar plates are then kept in an incubator at 37 ℃ for 2 days. After 2 days, the agar plates are then checked for any growth, and if there is the growth seen, gram stain and germ tube tests are done. It was seen that out of 100 samples collected, 40% were positive for Candida albicans, and 60% were negative for Candida albicans. And out of the percentage of positive samples, 26% were males, and 14% were females Candida albicans is the species most frequently implicated in the oral cavity, and it is important to take care of the oral cavity.

scholarly journals Secreted Aspartyl Proteinases and Interactions of Candida albicans with Human Endothelial Cells

1998 ◽  
Vol 66 (6) ◽  
pp. 3003-3005 ◽  
Author(s):  
Ashraf S. Ibrahim ◽  
Scott G. Filler ◽  
Dominique Sanglard ◽  
John E. Edwards ◽  
Bernhard Hube
Keyword(s):  
Endothelial Cells ◽  
Candida Albicans ◽  
Endothelial Cell ◽  
Cell Interactions ◽  
Human Endothelial Cells ◽  
Secreted Aspartyl Proteinase ◽  
Null Mutants ◽  
Aspartyl Proteinases

ABSTRACT The endothelial cell interactions of homozygous null mutants ofCandida albicans that were deficient in secreted aspartyl proteinase 1 (Sap1), Sap2, or Sap3 were investigated. Only Sap2 was found to contribute to the ability of C. albicans to damage endothelial cells and stimulate them to express E-selectin. None of the Saps studied appears to play a role in C. albicansadherence to endothelial cells.

scholarly journals Limited Role of Secreted Aspartyl Proteinases Sap1 to Sap6 in Candida albicans Virulence and Host Immune Response in Murine Hematogenously Disseminated Candidiasis

2010 ◽  
Vol 78 (11) ◽  
pp. 4839-4849 ◽  
Author(s):  
Alexandra Correia ◽  
Ulrich Lermann ◽  
Luzia Teixeira ◽  
Filipe Cerca ◽  
Sofia Botelho ◽  
...  
Keyword(s):  
Immune Response ◽  
Candida Albicans ◽  
Significant Role ◽  
Murine Model ◽  
Null Mutant ◽  
Wild Type ◽  
Survival Times ◽  
Disseminated Candidiasis ◽  
Mutant Strains ◽  
Aspartyl Proteinases

ABSTRACTCandida albicanssecreted aspartyl proteinases (Saps) are considered virulence-associated factors. Several members of the Sap family were claimed to play a significant role in the progression of candidiasis established by the hematogenous route. This assumption was based on the observed attenuated virulence ofsap-null mutant strains. However, the exclusive contribution ofSAPgenes to their attenuated phenotype was not unequivocally confirmed, as the Ura status of these mutant strains could also have contributed to the attenuation. In this study, we have reassessed the importance ofSAP1toSAP6in a murine model of hematogenously disseminated candidiasis usingsap-null mutant strains not affected in theirURA3gene expression and compared their virulence phenotypes with those of Ura-blastersapmutants. The median survival time of BALB/c mice intravenously infected with a mutant strain lackingSAP1toSAP3was equivalent to that of mice infected with wild-type strain SC5314, while those infected with mutant strains lackingSAP5showed slightly extended survival times. Nevertheless, no differences could be observed between the wild type and a Δsap456mutant in their abilities to invade mouse kidneys. Likewise, a deficiency inSAP4toSAP6had no noticeable impact on the immune response elicited in the spleens and kidneys ofC. albicans-infected mice. These results contrast with the behavior of equivalent Ura-blaster mutants, which presented a significant reduction in virulence. Our results suggest that Sap1 to Sap6 do not play a significant role inC. albicansvirulence in a murine model of hematogenously disseminated candidiasis and that, in this model, Sap1 to Sap3 are not necessary for successfulC. albicansinfection.

scholarly journals Effect of the crude extract of Eugenia uniflora in morphogenesis and secretion of hydrolytic enzymes in Candida albicans from the oral cavity of kidney transplant recipients

2015 ◽  
Vol 15 (1) ◽  
Author(s):  
Walicyranison Plinio Silva-Rocha ◽  
Vitor Luiz de Brito Lemos ◽  
Magda Rhayanny Assunção Ferreira ◽  
Luiz Alberto Lira Soares ◽  
Terezinha Inês Estivalet Svidzisnki ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Oral Cavity ◽  
Kidney Transplant ◽  
Crude Extract ◽  
Hydrolytic Enzymes ◽  
Transplant Recipients ◽  
Kidney Transplant Recipients ◽  
Eugenia Uniflora

Aspartyl proteinases of Candida albicans and their role in pathogenicity

2001 ◽  
Vol 39 (4) ◽  
pp. 303-313 ◽  
Author(s):  
F. de Bernardis ◽  
P. A. Sullivan ◽  
A. Cassone
Keyword(s):  
Candida Albicans ◽  
Aspartyl Proteinases

scholarly journals Mucosal IgA Prevents Commensal Candida albicans Dysbiosis in the Oral Cavity

2020 ◽  
Vol 11 ◽  
Author(s):  
Nicolas Millet ◽  
Norma V. Solis ◽  
Marc Swidergall
Keyword(s):  
Candida Albicans ◽  
Oral Cavity

scholarly journals Correlation between Azole Susceptibilities, Genotypes, and ERG11 Mutations in Candida albicans Isolates Associated with Vulvovaginal Candidiasis in China

2010 ◽  
Vol 54 (8) ◽  
pp. 3126-3131 ◽  
Author(s):  
Shu-Hua Ge ◽  
Zhe Wan ◽  
Juan Li ◽  
Jianping Xu ◽  
Ruo-Yu Li ◽  
...  
Keyword(s):  
Candida Albicans ◽  
Amino Acid ◽  
Oral Cavity ◽  
Target Gene ◽  
Chinese Women ◽  
Missense Mutations ◽  
Statistical Support ◽  
Strain Sc5314 ◽  
The Relationship ◽  
Susceptibility Patterns

ABSTRACT The relationship between susceptibilities to fluconazole and itraconazole and microsatellite CAI genotypes were examined from a total of 154 Candida albicans isolates (97 isolates causing vulvovaginitis in Chinese women and 6 vaginal isolates and 51 oral cavity isolates from asymptomatic carriers). The two dominant genotypes, CAI 30-45 (45 isolates) and CAI 32-46 (33 isolates), associated with vulvovaginitis showed significantly different azole susceptibility patterns with strong statistical support. CAI 32-46 isolates were usually less susceptible to both fluconazole and itraconazole than CAI 30-45 isolates and than the oral isolates with other diversified CAI genotypes. Remarkably different mutation patterns in the azole target gene ERG11 were correspondingly observed among C. albicans isolates representing different genotypes and sources. Isolates with the same or similar CAI genotypes usually possessed identical or phylogenetically closely related ERG11 sequences. Loss of heterozygosity in ERG11 was observed in all the CAI 32-46 isolates but not in the CAI 30-45 isolates and most of the oral isolates sequenced. Compared with the ERG11 sequence of strain SC5314 (X13296), two homozygous missense mutations (G487T and T916C) leading to two amino acid changes (A114S and Y257H) in Erg11p were found in CAI 32-46 isolates. The correlation between azole susceptibility and C. albicans genotype may be of potential therapeutic significance.

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