Synthesis and physical properties of fatty acid derivatives containing amino, amido functions and L-amino acid residues

1993 ◽  
Author(s):  
Wai-yin, David Leung
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Physical Properties ◽  
Amino Acid Residues ◽  
Fatty Acid Derivatives

Evidence for Biosynthetical Equivalence of the Epimeric Isoleucine Residues in Angolide

1972 ◽  
Vol 50 (4) ◽  
pp. 428-439 ◽  
Author(s):  
R. O. Okotore ◽  
D. W. Russell
Keyword(s):  
Amino Acid ◽  
Physical Properties ◽  
Acid Hydrolysis ◽  
Column Chromatography ◽  
Mass Spectra ◽  
Mathematical Treatment ◽  
Amino Acid Residues ◽  
Analytical Results ◽  
Cyclic Intermediate

Pithomyces sacchari was grown on media containing L-valine. Instead of the metabolite angolide (cyclo-L-α-hydroxyisovaleryl1-erythro-L-isoleucyl2-L-α-hydroxyisovaleryl3-threo-D-isoleucyl4), synthesized on un-supplemented media, it produced a mixture with very similar physical properties. Acid hydrolysis liberated erythro-L- and threo-D-isoleucines (1:1), and DL-valine in amounts proportional to the exogenous valine concentration; L-α-hydroxyisovaleric acid was the only other component. Radioactivity from L-valine-U-14C was incorporated without dilution into valine, and with extensive dilution into the hydroxy acid.Partial fractionation of the mixture was achieved by column chromatography. Mass spectra of two fractions, containing different proportions of valine, were compared with the spectrum of angolide. A simple mathematical treatment showed that the fractions contained angolide, its divaline homologue, and a homologue with only one isoleucine replaced by valine. This must be a 1: 1 mixture of the two possible isomers, since any deviation from this ratio is inconsistent with the analytical results. It follows that the erythro-L- and the threo-D-isoleucine residues of angolide are equivalent with respect to their replacement by valine. It is inferred that angolide biosynthesis involves a cyclic intermediate containing both amino acid residues in the L configuration.

scholarly journals Identification of amino acid residues that determine the substrate specificity of mammalian membrane-bound front-end fatty acid desaturases

2015 ◽  
Vol 57 (1) ◽  
pp. 89-99 ◽  
Author(s):  
Kenshi Watanabe ◽  
Makoto Ohno ◽  
Masahiro Taguchi ◽  
Seiji Kawamoto ◽  
Kazuhisa Ono ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Substrate Specificity ◽  
Amino Acid Residues ◽  
Fatty Acid Desaturases ◽  
Front End ◽  
Membrane Bound

The role of C-terminal amino acid residues of a Δ6-fatty acid desaturase from blackcurrant

2013 ◽  
Vol 431 (4) ◽  
pp. 675-679 ◽  
Author(s):  
Li-Ying Song ◽  
Wan-Xiang Lu ◽  
Jun Hu ◽  
Wei-Bo Yin ◽  
Yu-Hong Chen ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Fatty Acid Desaturase ◽  
Amino Acid Residues ◽  
Terminal Amino Acid ◽  
Terminal Amino

Targeted mutagenesis of a fatty acid Δ6-desaturase from Mucor rouxii: Role of amino acid residues adjacent to histidine-rich motif II

2006 ◽  
Vol 339 (4) ◽  
pp. 1029-1034 ◽  
Author(s):  
Sutthicha Na-Ranong ◽  
Kobkul Laoteng ◽  
Prasat Kittakoop ◽  
Morakot Tanticharoen ◽  
Supapon Cheevadhanarak
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Targeted Mutagenesis ◽  
Amino Acid Residues ◽  
Mucor Rouxii ◽  
Δ6 Desaturase
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