Identification of biological inhibitors of the mammalian thioredoxin system

1999 ◽  
Author(s):  
Ka-wai Siu
Keyword(s):  
Thioredoxin System ◽  
Biological Inhibitors

scholarly journals One Century of Study: What We Learned about Paracoccidioides and How This Pathogen Contributed to Advances in Antifungal Therapy

2021 ◽  
Vol 7 (2) ◽  
pp. 106
Author(s):  
Erika Seki Kioshima ◽  
Patrícia de Souza Bonfim de Mendonça ◽  
Marcus de Melo Teixeira ◽  
Isis Regina Grenier Capoci ◽  
André Amaral ◽  
...  
Keyword(s):  
Latin America ◽  
Life Cycle ◽  
Antifungal Activity ◽  
Molecular Biology ◽  
Antifungal Therapy ◽  
High Toxicity ◽  
Development Stages ◽  
Thioredoxin System ◽  
Nanostructured Systems ◽  
Advanced Development

Paracoccidioidomycosis (PCM) is a notable fungal infection restricted to Latin America. Since the first description of the disease by Lutz up to the present day, Brazilian researchers have contributed to the understanding of the life cycle of this pathogen and provided the possibility of new targets for antifungal therapy based on the structural and functional genomics of Paracoccidioides. In this context, in silico approaches have selected molecules that act on specific targets, such as the thioredoxin system, with promising antifungal activity against Paracoccidioides. Some of these are already in advanced development stages. In addition, the application of nanostructured systems has addressed issues related to the high toxicity of conventional PCM therapy. Thus, the contribution of molecular biology and biotechnology to the advances achieved is unquestionable. However, it is still necessary to transcend the boundaries of synthetic chemistry, pharmaco-technics, and pharmacodynamics, aiming to turn promising molecules into newly available drugs for the treatment of fungal diseases.

scholarly journals An unusual thioredoxin system in the facultative parasite Acanthamoeba castellanii

2021 ◽  
Vol 78 (7) ◽  
pp. 3673-3689
Author(s):  
David Leitsch ◽  
Alvie Loufouma Mbouaka ◽  
Martina Köhsler ◽  
Norbert Müller ◽  
Julia Walochnik
Keyword(s):  
Oxidative Stress ◽  
Stop Codon ◽  
Acanthamoeba Castellanii ◽  
Methionine Sulfoxide ◽  
Redox System ◽  
Methionine Sulfoxide Reductase ◽  
Methionine Sulfoxide Reductase A ◽  
Protein Levels ◽  
Thioredoxin System ◽  
Facultative Parasite

AbstractThe free-living amoeba Acanthamoeba castellanii occurs worldwide in soil and water and feeds on bacteria and other microorganisms. It is, however, also a facultative parasite and can cause serious infections in humans. The annotated genome of A. castellanii (strain Neff) suggests the presence of two different thioredoxin reductases (TrxR), of which one is of the small bacterial type and the other of the large vertebrate type. This combination is highly unusual. Similar to vertebrate TrxRases, the gene coding for the large TrxR in A. castellanii contains a UGA stop codon at the C-terminal active site, suggesting the presence of selenocysteine. We characterized the thioredoxin system in A. castellanii in conjunction with glutathione reductase (GR), to obtain a more complete understanding of the redox system in A. castellanii and the roles of its components in the response to oxidative stress. Both TrxRases localize to the cytoplasm, whereas GR localizes to the cytoplasm and the large organelle fraction. We could only identify one thioredoxin (Trx-1) to be indeed reduced by one of the TrxRases, i.e., by the small TrxR. This thioredoxin, in turn, could reduce one of the two peroxiredoxins tested and also methionine sulfoxide reductase A (MsrA). Upon exposure to hydrogen peroxide and diamide, only the small TrxR was upregulated in expression at the mRNA and protein levels, but not the large TrxR. Our results show that the small TrxR is involved in the A. castellanii’s response to oxidative stress. The role of the large TrxR, however, remains elusive.

scholarly journals Cytosolic Thioredoxin System Facilitates the Import of Mitochondrial Small Tim Proteins

2012 ◽  
Vol 53 ◽  
pp. S214
Author(s):  
R. Durigon ◽  
Q. Wang ◽  
E. Ceh Pavia ◽  
C.M. Grant ◽  
H. Lu⁎
Keyword(s):  
Thioredoxin System ◽  
Tim Proteins

The thioredoxin system fromStreptomyces coelicolor

2006 ◽  
Vol 46 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Petra Štefanková ◽  
Dušan Perečko ◽  
Imrich Barák ◽  
Marta Kollárová
Keyword(s):  
Thioredoxin System

scholarly journals Degradation of somatomedins by the thioredoxin system

FEBS Letters ◽  
1985 ◽  
Vol 183 (1) ◽  
pp. 52-54 ◽  
Author(s):  
Gösta Enberg ◽  
Arne Holmgren
Keyword(s):  
Thioredoxin System

scholarly journals Antioxidant Activities and Selenogene Transcription in the European Sea Bass (Dicentrarchus labrax) Liver Depend, in a Non-linear Manner, on the Se/Hg Molar Ratio of the Feeds

2021 ◽  
Author(s):  
Marinelle Espino ◽  
Harkaitz Eguiraun ◽  
Oihane Diaz de Cerio ◽  
José Antonio Carrero ◽  
Nestor Etxebarria ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Dicentrarchus Labrax ◽  
Thioredoxin Reductase ◽  
Sea Bass ◽  
Molar Ratio ◽  
Antioxidant Systems ◽  
European Sea Bass ◽  
Thioredoxin System ◽  
Non Linear ◽  
Linear Manner

AbstractFeeding 3.9 and 6.7 mg Hg/kg (Se/Hg molar ratios of 0.8 and 0.4, respectively) for 14 days negatively affected Dicentrarchus labrax growth and total DNTB- and thioredoxin-reductase (TrxR) activities and the transcription of four redox genes (txn1, gpx1, txnrd3, and txnrd2) in the liver, but a diet with 0.5 mg Hg/kg (Se/Hg molar ratio 6.6) slightly increased both reductase activities and the transcription of txn1, gpx1, and txnrd2. Feeding 6.7 mg Hg/kg for 53 days downregulated the genes of the thioredoxin system (txn1, txnrd3, and txnrd2) but upregulated gpx1, confirming the previously proposed complementarity among the antioxidant systems. Substitution of 20% of the feed by thawed white fish (hake) slightly counteracted the negative effects of Hg. The effects were not statistically significant and were dependent, in a non-linear manner, on the Se/Hg molar ratio of the feed but not on its Hg concentration. These results stress the need to consider the Se/Hg molar ratio of the feed/food when evaluating the toxicity of Hg.

scholarly journals The Involvement of the Thioredoxin System in the Reduction of Methionine Sulfoxide and Sulfate

1970 ◽  
Vol 245 (9) ◽  
pp. 2371-2374 ◽  
Author(s):  
Pedro Gonzalez Porqué ◽  
Astor Baldesten ◽  
Peter Reichard
Keyword(s):  
Methionine Sulfoxide ◽  
Thioredoxin System

Properties of the endogenous components of the thioredoxin system in the psychrophilic eubacterium Pseudoalteromonas haloplanktis TAC 125

Extremophiles ◽  
2012 ◽  
Vol 16 (3) ◽  
pp. 539-552 ◽  
Author(s):  
Patrizia Falasca ◽  
Giovanna Evangelista ◽  
Roberta Cotugno ◽  
Salvatore Marco ◽  
Mariorosario Masullo ◽  
...  
Keyword(s):  
Pseudoalteromonas Haloplanktis ◽  
Thioredoxin System
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