Changes in cytodifferentiation of the dunning prostatic adenocarcinoma induced by neonatal rat seminal vesicle mesenchyme

1998 ◽  
Author(s):  
Xiaofeng Lu
Keyword(s):  
Seminal Vesicle ◽  
Prostatic Adenocarcinoma ◽  
Neonatal Rat ◽  
Rat Seminal Vesicle

scholarly journals Isolation and partial purification of the major abundant class rat seminal vesicle poly(A+-messenger RNA

1979 ◽  
Vol 7 (6) ◽  
pp. 1553-1565 ◽  
Author(s):  
Per-Erik Mansson ◽  
Donald B. Carter ◽  
Alan B. Silverberg ◽  
Douglas B. Tully ◽  
Stephen E. Hairis
Keyword(s):  
Seminal Vesicle ◽  
Messenger Rna ◽  
Partial Purification ◽  
Rat Seminal Vesicle

Induction of functional cytodifferentiation in the epithelium of tissue recombinants. I. Homotypic seminal vesicle recombinants

Development ◽  
1989 ◽  
Vol 106 (2) ◽  
pp. 219-234 ◽  
Author(s):  
S.J. Higgins ◽  
P. Young ◽  
J.R. Brody ◽  
G.R. Cunha
Keyword(s):  
Seminal Vesicle ◽  
Time Course ◽  
Full Range ◽  
Seminal Vesicles ◽  
Neonatal Rat ◽  
Secretory Proteins ◽  
Functional Variation ◽  
Adult Rats ◽  
Normal Functional

Functional cytodifferentiation of seminal vesicle epithelium was investigated in tissue recombinants. Neonatal rat and mouse seminal vesicles were separated into epithelium and mesenchyme using trypsin. Epithelium and mesenchyme were then recombined in vitro to form interspecific rat/mouse homotypic recombinants. Growth as renal grafts in adult male athymic mice resulted in seminal vesicle morphogenesis in 70% of the recombinants (the remaining 30% failed to grow). Functional cytodifferentiation was judged by the expression of the major androgen-dependent secretory proteins characteristic of the seminal vesicles of adult rats and mice. Antibodies specific for each of these proteins were used to screen tissue sections by immunocytochemistry and to probe protein extracts by immunoblotting techniques. The heterospecific recombinants synthesized the full range of seminal vesicle secretory proteins that typifies the species providing the epithelium of the recombinant, not the mesenchyme. There was little functional variation between individual recombinants. The time course of development corresponded to that of intact neonatal seminal vesicles grown under the same conditions. Morphogenesis and functional cytodifferentiation were not evident after one week, but were well advanced after two weeks. Seminal vesicle recombinants grown for three weeks were indistinguishable morphologically and functionally from normal adult seminal vesicles. In addition, the ability of adult seminal vesicle epithelium to be induced to proliferate was examined. In association with neonatal seminal vesicle mesenchyme, the epithelium of the adult seminal vesicle proliferated and retained its normal functional activity. Thus, seminal vesicle functional cytodifferentiation can be faithfully reproduced in homotypic tissue recombinants. The methods used in this study will be used to investigate seminal vesicle development in instructive inductions of heterotypic epithelia.

In vivo inhibition of cell-mediated and humoral immune responses to cellular antigens by SV-IV, a major protein secreted from the rat seminal vesicle epithelium

1995 ◽  
Vol 28 (1) ◽  
pp. 15-30 ◽  
Author(s):  
Caterina Romano-Carratelli ◽  
Marilena Galdiero ◽  
Immacolata Nuzzo ◽  
Concetta Bentivoglio ◽  
Raffaele Porta ◽  
...  
Keyword(s):  
Immune Responses ◽  
Seminal Vesicle ◽  
Major Protein ◽  
Humoral Immune ◽  
Humoral Immune Responses ◽  
Rat Seminal Vesicle

scholarly journals The 11S rat seminal vesicle mRNA directs thein vitrosynthesis of two precursors of the major secretory protein IV

1984 ◽  
Vol 12 (4) ◽  
pp. 1901-1910 ◽  
Author(s):  
S. Metafora ◽  
J. Guardiola ◽  
G. Paonessa ◽  
P. Abrescia
Keyword(s):  
Seminal Vesicle ◽  
Secretory Protein ◽  
Rat Seminal Vesicle
Sign in / Sign up

Export Citation Format

Share Document