Isolation and characterization of environmental vibrio species from Mai Po Nature Reserve, Hong Kong

2003 ◽  
Author(s):  
Yanling Wang
Keyword(s):  
Hong Kong ◽  
Nature Reserve ◽  
Vibrio Species ◽  
Isolation And Characterization

A Longitudinal Analysis of Methicillin-ResistantStaphylococcus aureusin a Hong Kong Teaching Hospital

2004 ◽  
Vol 25 (2) ◽  
pp. 126-129 ◽  
Author(s):  
Margaret Ip ◽  
D. J. Lyon ◽  
F. Chio ◽  
A. F. Cheng
Keyword(s):  
Hong Kong ◽  
Teaching Hospital ◽  
Dna Typing ◽  
Type A ◽  
University Teaching ◽  
Pfge Type ◽  
Type B ◽  
Isolation And Characterization ◽  
Surgical Units

AbstractObjectives:To review the incidence and trends of MRSA during a 12-year (1989-2000) period at a university teaching hospital and the relationship between strain distribution by antibiogram and molecular typing.Design:Retrospective review of laboratory-based surveillance records on MRSA isolation and characterization of strains by antimicrobial susceptibility and PFGE. A patient episode was counted at the time when MRSA was first isolated.Setting:A 1,350-bed university teaching hospital in Hong Kong.Patients:Those with clinical isolates of MRSA.Results:During 1989 to 2000, the hospital recorded 1,203,175 deaths and discharges (D&D) and encountered 5,707 patient episodes of new MRSA isolation. The overall incidence of patient episodes of MRSA was 0.47/100 D&D. In 1989, the incidence was 0.81/100 D&D and fell to a low of 0.33/100 D&D in 1995, but then rose to 0.50/100 D&D in 2000. Antibiogram and DNA typing identified 5 major types. PFGE type A constituted 68% (211/312) of isolates and was present throughout the 12-year period. PFGE type B constituted 13% (40/312) of isolates and was only present from 1995 to 2000. These isolates form a distinct clone and had unique antibiotic resistance profiles.Conclusions:The study showed the establishment of a dominant MRSA clone (PFGE type A group) in the intensive care, medical, and surgical units and the appearance of a new MRSA strain in 1995 (PFGE type B), which partly explained the rise in incidence of MRSA cases and a disproportionate rise in MRSA bacteremia from 1995 to 2000.

Isolation and characterization of potentially pathogenic Vibrio species in a temperate, higher latitude hotspot

2020 ◽  
Vol 12 (4) ◽  
pp. 424-434 ◽  
Author(s):  
Charlotte L. Ford ◽  
Andy Powell ◽  
Dawn Yan Lam Lau ◽  
Andrew D. Turner ◽  
Monika Dhanji‐Rapkova ◽  
...  
Keyword(s):  
Vibrio Species ◽  
Isolation And Characterization ◽  
Pathogenic Vibrio

scholarly journals High incidence of plasmids in marine Vibrio species isolated from Mai Po Nature Reserve of Hong Kong

Ecotoxicology ◽  
2012 ◽  
Vol 21 (6) ◽  
pp. 1661-1668 ◽  
Author(s):  
Ruifu Zhang ◽  
Li Pan ◽  
Zhenye Zhao ◽  
Ji-Dong Gu
Keyword(s):  
Hong Kong ◽  
Nature Reserve ◽  
Vibrio Species ◽  
High Incidence ◽  
Marine Vibrio

scholarly journals Isolation and Characterization of Infectious Vibrio Species from the Whiteleg Shrimp: Litopenaeus vannamei

2020 ◽  
Vol 2 (1) ◽  
pp. 36
Keyword(s):  
Litopenaeus Vannamei ◽  
Economic Loss ◽  
Vibrio Species ◽  
Shrimp Farming ◽  
Microbial Risk Assessment ◽  
Microbial Risk ◽  
Isolation And Characterization ◽  
Vibrio Campbellii ◽  
Vibrio Spp

Aquaculture shrimp farming has serious problems with diseases caused by viruses and bacteria, which lead to severe economic loss. The genus Vibrio is a group of facultative anaerobic microbes, most frequently found in aquatic environments and marine hosts. The Gram-negative genus Vibrio is one of the most important classes of bacterial pathogens in aquaculture systems and is a key cause of high mortality. The isolation, identification, and molecular characterization of Vibrio spp are studied from infected shrimp Litopenaeus vannamei. The microorganisms were isolated from the tissues of a white gut infected shrimp collected from diseased aquaculture ponds. After subculturing, microbial isolates were obtained using TCBS agar plates for vibrio selection, and the biochemical key was developed. Meanwhile, 11 isolates of bacteria belonging to the Vibrionaceae family have been identified in this study. For these, six Vibrio species-related isolates are responsible for vibriosis in the shrimp. These included Vibrio harveyi, Vibrio campbellii, Vibrio parahaemolyticus, Vibrio owensii, Vibrio rotiferianus, and Vibrio alginolyticus. The extraction of DNA was carried out with a QIAamp DNA Mini Kit (QIAGEN). The extracted DNA was analyzed using an electrophoresis of 1 % agarose gel, the consistency and quantity of Vibrio spp's gDNA. NanoDropTM 2000 spectrophotometer (Thermo Scientific) was used for determination. In addition, the Agilent 2100 Bioanalyzer (Agilent Technologies) has been used to verify the gDNA libraries' validity, purity, concentration, and scale. In addition, Vibrio spp, a phylogenetic tree focused on maximum likelihood and bootstrapping, was performed using the MEGA version to examine the evolutionary phylogenetic relationship between the strains. This study's main objective would be useful in integrating strain variation in predicting microbiology and microbial risk assessment and may provide scientific guidelines for major Vibrio strains that may be responsible for vibriosis infection in the management of shrimp aquaculture disease.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document