scholarly journals Morphological Study on Differentiation of Hepatocytic Stem Cell by Intrasplenic Transplantation after Partial Hepatectomy

2002 ◽  
Vol 12 (3) ◽  
pp. 294-305
Keyword(s):  
Stem Cell ◽  
Partial Hepatectomy ◽  
Morphological Study ◽  
Intrasplenic Transplantation

A study on intrasplenic transplantation of hepatocytic STEM cell in rats with partial hepatectomy

2003 ◽  
Vol 124 (4) ◽  
pp. A662
Author(s):  
Jung Myung Chung ◽  
Jae Kyung Lee ◽  
Eun Taek Park ◽  
Sang Hyuk Lee ◽  
Jae Hong Park ◽  
...  
Keyword(s):  
Stem Cell ◽  
Partial Hepatectomy ◽  
Intrasplenic Transplantation

scholarly journals The adipose-derived mesenchymal stem cell secretome promotes hepatic regeneration in miniature pigs after liver ischaemia-reperfusion combined with partial resection

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Zhihui Jiao ◽  
Yajun Ma ◽  
Qianzhen Zhang ◽  
Yue Wang ◽  
Tao Liu ◽  
...  
Keyword(s):  
Stem Cell ◽  
Liver Function ◽  
Liver Regeneration ◽  
Partial Hepatectomy ◽  
Liver Tissue ◽  
Expression Levels ◽  
Miniature Pigs ◽  
Ischaemia Reperfusion ◽  
Tnf Α ◽  
Liver Ischaemia

Abstract Background Hepatic ischaemia-reperfusion injury (HIRI) is inevitable in complicated liver surgery and is a major factor leading to postoperative complications and liver dysfunction. Studies have shown that the paracrine mechanisms of stem cell may be essential to tissue repair and functional improvement after transplantation. However, the role of the adipose-derived mesenchymal stem cell secretome (ASC-secretome) in liver regeneration in large animals remains to be determined. Methods Twenty-four miniature pigs were subjected to laparoscopic liver ischaemia-reperfusion combined with partial hepatectomy and divided into the following four groups: the saline group, the DMEM group, the ASC group and the ASC-secretome group. Serum and liver tissue samples were collected before the operation and at 1, 3 and 7 days after the operation, and changes in tissue pathology, serum inflammation, liver function, angiogenesis-related factors and liver tissue regeneration-related genes and proteins were evaluated. Results Detailed histological analysis showed that ASCs and the ASC-secretome changed pathological damage to liver tissue after liver ischaemia-reperfusion combined with partial hepatectomy (1 and 3 days: p < 0.01). Compared with the saline and DMEM control groups, the ASC-secretome group had significantly reduced expression levels of ALP (1 and 3 days: p < 0.05), ALT (1 day: p < 0.01; 3 days: p < 0.05) and AST (1 and 3 days: p < 0.01), which promoted the recovery of liver function. Moreover, detection of the expression levels of TNF-α and IL-1β (1 day: p < 0.01; 3 days: p < 0.05), IL-6 (1 and 3 days: p < 0.05) and IL-10 (1 and 3 days: p < 0.01) in serum confirmed that the ASC-secretome had obvious anti-inflammatory effects. In addition, the ASC-secretome increased the expression levels of ANG-1 (3 days: p < 0.01), ANG-2 (3 and 7 days: p < 0.01) and VEGF (1 and 7 days: p < 0.05; 3 days: p < 0.01) and promoted angiogenesis during liver regeneration. Moreover, it promoted the mRNA expression of HGF and Cyclin D1 (1 and 3 days: p < 0.01); increased the levels of p-STAT3 (1 and 3 days: p < 0.01), PCNA and Ki67 (1 and 3 days: p < 0.01; 7 days: p < 0.05); inhibited the negative feedback of SOCS3 (1 and 3 days: p < 0.01); and decreased the mRNA expression of TGF-β (3 days: p < 0.01). The cytokines and growth factors detected in the ASC-secretome included TNF-α, IL-6, IL-1β, ANG-1, ANG-2, VEGF and b-FGF. Conclusion The ASC-secretome alleviates the inflammatory response induced by ischaemia-reperfusion combined with partial hepatectomy in miniature pigs and promotes liver regeneration.

scholarly journals CD39 Modulates Hematopoietic Stem Cell Recruitment and Promotes Liver Regeneration in Mice and Humans After Partial Hepatectomy

2013 ◽  
Vol 257 (4) ◽  
pp. 693-701 ◽  
Author(s):  
Moritz Schmelzle ◽  
Constanze Duhme ◽  
Wolfgang Junger ◽  
Steven D. Salhanick ◽  
Yu Chen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Liver Regeneration ◽  
Hematopoietic Stem Cell ◽  
Partial Hepatectomy ◽  
Hematopoietic Stem ◽  
Cell Recruitment

CD133+ stem cell mobilization after partial hepatectomy depends on resection extent and underlying disease

2011 ◽  
Vol 43 (2) ◽  
pp. 147-154 ◽  
Author(s):  
Maria Assunta Zocco ◽  
Anna Chiara Piscaglia ◽  
Felice Giuliante ◽  
Vincenzo Arena ◽  
Marialuisa Novi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Partial Hepatectomy ◽  
Underlying Disease ◽  
Stem Cell Mobilization ◽  
Cell Mobilization

Adult-Derived Human Liver Mesenchymal-Like Cells as a Potential Progenitor Reservoir of Hepatocytes?

2007 ◽  
Vol 16 (7) ◽  
pp. 717-728 ◽  
Author(s):  
Mustapha Najimi ◽  
Dung Ngoc Khuu ◽  
Philippe Antoine Lysy ◽  
Nawal Jazouli ◽  
Jorge Abarca ◽  
...  
Keyword(s):  
Stem Cell ◽  
Human Liver ◽  
Adult Stem Cells ◽  
Scid Mice ◽  
Differentiation Potential ◽  
Adult Human ◽  
Cell Source ◽  
Intrasplenic Transplantation

It is currently accepted that adult tissues may develop and maintain their own stem cell pools. Because of their higher safety profile, adult stem cells may represent an ideal candidate cell source to be used for liver cell therapies. We therefore evaluated the differentiation potential of mesenchymal-like cells isolated from adult human livers. Mesenchymal-like cells were isolated from enzymatically digested adult human liver and expanded in vitro. Cell characterization was performed using flow cytometry, RT-PCR, and immunofluorescence, whereas the differentiation potential was evaluated both in vitro after incubation with specific media and in vivo after intrasplenic transplantation of uPA+/+-SCID and SCID mice. Adult-derived human liver mesenchymal-like cells expressed both hepatic and mesenchymal markers among which albumin, CYP3A4, vimentin, and α-smooth muscle actin. In vitro differentiation studies demonstrated that these mesenchymal-like cells are preferentially determined to differentiate into hepatocyte-like cells. Ten weeks following intrasplenic transplantation into uPA+/+-SCID mice, recipient livers showed the presence of human hepatocytic cell nodules positive for human albumin, prealbumin, and α-fetoprotein. In SCID transplanted liver mice, human hepatocyte-like cells were mostly found near vascular structures 56 days posttransplantation. In conclusion, the ability of isolated adult-derived liver mesenchymal stem-like cells to proliferate and differentiate into hepatocyte-like cells both in vitro and in vivo leads to propose them as an attractive expandable cell source for stem cell therapy in human liver diseases.

scholarly journals Intrasplenic Transplantation of Hepatocytes After Partial Hepatectomy in NOD.SCID Mice

10.3791/56018 ◽  
2018 ◽  
Author(s):  
Barun Das ◽  
Jashdeep Bhattacharjee ◽  
Preeti ◽  
Alaknanda Mishra ◽  
Kshama Jain ◽  
...  
Keyword(s):  
Partial Hepatectomy ◽  
Intrasplenic Transplantation
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