scholarly journals Grapevine Virus E Detected in Ohio Vineyards

2020 ◽  
pp. catalyst.2020.20006
Author(s):  
X.-L. Yao ◽  
L.L. Domier ◽  
F. Qu ◽  
M.L. Lewis Ivey
Keyword(s):  
Grapevine Virus

scholarly journals Evidence of Grapevine leafroll associated virus-1–3, Grapevine fleck virus and Grapevine virus B Occurring in Himachal Pradesh, India

2013 ◽  
Vol 24 (1) ◽  
pp. 66-69 ◽  
Author(s):  
Surender Kumar ◽  
Lakhmir Singh ◽  
Luca Ferretti ◽  
Marina Barba ◽  
Aijaz A. Zaidi ◽  
...  
Keyword(s):  
Himachal Pradesh ◽  
Grapevine Virus ◽  
Grapevine Virus B

Identification of mealybugs, soft scale insects and their predators in vineyards across the savannah agro-ecological region of Nigeria

2021 ◽  
Vol 18 (1) ◽  
pp. 27-32
Author(s):  
A.M. Zongoma ◽  
D.B. Dangora ◽  
M. Sétamou ◽  
M.D. Alegbejo ◽  
O.J. Alabi
Keyword(s):  
Natural Enemies ◽  
Integrated Management ◽  
Scale Insects ◽  
Disease Spread ◽  
Universal Primers ◽  
Grapevine Virus ◽  
Maconellicoccus Hirsutus ◽  
Anagyrus Kamali ◽  
Leafroll Disease ◽  
Ecological Region

Insect-vectored viruses are a major threat to grapevine production but there is a dearth of information on the occurrence and distribution of key grapevine pests in Nigeria. The recent detection of grapevine leafroll associated virus-1 (GLRaV-1), a known insect-vectored ampelovirus, in Nigeria elevates the importance of the identification of its potential vectors as a precursor to assessing the risk of grapevine leafroll disease spread. This study was conducted to determine the occurrence and diversity of potential vectors of grapevine viruses and their natural enemies in vineyards across the savannah agro-ecological region of Nigeria. Forty vineyard and nursery locations were surveyed during 2016 and 45 arthropod samples were collected. The samples were first morphologically identified, and DNA barcoding was conducted on a subset of 16 representative samples using universal primers specific to the Mitochondrial Cytochrome Oxidase subunit I (mtCOI) gene of most insects. The results indicated the presence of two species of scale insects (Parasaissetia nigra and Saissetia coffeae) and two mealybug species (Maconellicoccus hirsutus and Ferrisia virgata), some ofwhich are potential grapevine virus vectors, in Nigerian vineyards. In addition, the natural enemies of these insect species were detected which includes three species of parasitoids (Anagyrus kamali, Anagyrus pseudococci and Encarsia inaron) and one predator (Hyperaspidius mimus). While the detection of mealybugs and scale insects underscore the risk of vector-mediated virus spread in Nigerian vineyards, the identification of their natural enemies indicates presence of natural biological control agents to facilitate an integrated management of economically important grapevine virus diseases in the country. Keywords: Mealybugs; scale insects; parasitoids and predators; insect vectors; grapevine viruses.

Prevalence and genetic diversity of viruses associated with rugose wood complex in Greek vineyards

Plant Disease ◽  
2021 ◽  
Author(s):  
Chrysoula Orfanidou ◽  
Kalliopi Moraki ◽  
Polina Panailidou ◽  
Leonidas Lotos ◽  
Asimina T Katsiani ◽  
...  
Keyword(s):  
Phylogenetic Trees ◽  
First Record ◽  
Grapevine Virus ◽  
Multiple Introductions ◽  
Worldwide Distribution ◽  
Grapevine Virus A ◽  
Transmission Modes ◽  
Virus Incidence ◽  
Grapevine Virus B ◽  
Rupestris Stem Pitting

Rugose wood is one of the most important disease syndromes of grapevine and it has been associated with at least three viruses: grapevine rupestris stem pitting associated virus (GRSPaV), grapevine virus A (GVA) and grapevine virus B (GVB). All three viruses show a worldwide distribution pattern, and their genetic composition has been the focus of extensive research over the past years. Despite their first record in Greece almost 20 years ago, there is a lack of knowledge on the distribution and genetic variability of their populations in Greek vineyards. In this context, we investigated the distribution of GRSPaV, GVA and GVB in rootstocks, self-rooted and grafted grapevine cultivars, originating from different geographic regions that are representing important viticultural areas of Greece. Three new RT-PCR assays were developed for the reliable detection of GRSPaV, GVA and GVB. Our results indicated that GVA is the most prevalent in Greek vineyards, followed by GRSPaV and GVB. However, virus incidence differed among self-rooted and grafted grapevine cultivars or rootstocks tested. Selected isolates from each virus were further molecularly characterized to determine their phylogenetic relationships. All three viruses exhibited high nucleotide diversity, which was depicted in the constructed phylogenetic trees. Isolates from Greece were placed in various phylogroups, reinforcing the scenario of multiple introductions of GVA, GVB and GRSPaV in Greece and highlighting the effect of different transmission modes in the evolutionary course of the three viruses.

scholarly journals Detection and coat protein gene characterization of an isolate of Grapevine virus B from corky bark-affected grapevines in Southern Brazil

2002 ◽  
Vol 27 (3) ◽  
pp. 279-284 ◽  
Author(s):  
OSMAR NICKEL ◽  
THOR V. M. FAJARDO ◽  
FRANCISCO J. L. ARAGÃO ◽  
CÉSAR M. CHAGAS ◽  
GILMAR B. KUHN
Keyword(s):  
Coat Protein ◽  
Coat Protein Gene ◽  
Protein Gene ◽  
Amino Acid Sequences ◽  
Grapevine Virus ◽  
Leaf Extracts ◽  
Western Blots ◽  
Gene Characterization ◽  
Gene Coding ◽  
Grapevine Virus B

An isolate of Grapevine virus B (GVB), obtained by indexing Vitis labrusca and V. vinifera grapevines on the indicator LN33, was transmitted mechanically to several Nicotiana species. The virus was partially purified from N. cavicola and the coat protein estimated at 23 kDa by SDS-PAGE. In negatively stained leaf extracts of experimentally inoculated N. cavicola and N. occidentalis, flexuous particles with cross banding were observed, predominantly measuring 750-770 x 12 nm, with a modal length of 760 nm. Decoration indicated a clear, positive reaction against AS-GVB. In DAS-ELISA, GVB was detected in N. cavicola and grapevine extracts, and Western blots showed homologous and cross reaction of GVB and GVA antisera with GVB coat protein. Using specific primers for GVB, a fragment of 594 bp, comprising the coat protein gene coding for 197 amino acids, was amplified by RT-PCR with viral RNA extracted from GVB-infected N. occidentalis. The nucleotide and the deduced amino acid sequences of the coat protein gene showed high identities with Italian and Japanese isolates of GVB.

scholarly journals Production of polyclonal antisera using recombinant coat proteins of Grapevine leafroll-associated virus 2 and Grapevine virus B

2008 ◽  
Vol 43 (10) ◽  
pp. 1405-1411 ◽  
Author(s):  
Paula Radaelli ◽  
Thor Vinícius Martins Fajardo ◽  
Osmar Nickel ◽  
Marcelo Eiras ◽  
Gilvan Pio-Ribeiro
Keyword(s):  
Virus Disease ◽  
Disease Diagnosis ◽  
Coat Proteins ◽  
Grapevine Virus ◽  
Western Blots ◽  
E Coli ◽  
Sds Page ◽  
Grapevine Virus B ◽  
Polyclonal Antisera ◽  
Infected Grapevine

The objective of this work was to produce and characterize specific antisera against Brazilian isolates of Grapevine leafroll-associated virus 2 (GLRaV-2) and Grapevine virus B (GVB), developed from expressed coat proteins (CPs) in Escherichia coli, and to test their possible use for the detection of these two viruses in diseased grapevines. The coat protein (CP) genes were RT-PCR-amplified, cloned and sequenced. The CP genes were subsequently subcloned, and the recombinant plasmids were used to transform E. coli cells and express the coat proteins. The recombinant coat proteins were purified, and their identities were confirmed by SDS-PAGE and Western blot and used for rabbit immunizations. Antisera raised against these proteins were able to recognize the corresponding recombinant proteins in Western blots and to detect GLRaV-2 and GVB in infected grapevine tissues, by indirect ELISA, discriminating healthy and infected grapevines with absorbances (A405) of 0.08/1.15 and 0.12/1.30, respectively. Expressing CP genes can yield high amount of viral protein with high antigenicity, and GLRaV-2 and GVB antisera obtained in this study can allow reliable virus disease diagnosis.

scholarly journals First Report of Grapevine fleck virus and Grapevine virus E in Grapevine in Korea

Plant Disease ◽  
2017 ◽  
Vol 101 (6) ◽  
pp. 1069
Author(s):  
Y. Jo ◽  
M. K. Song ◽  
H. Choi ◽  
J. S. Park ◽  
J. W. Lee ◽  
...  
Keyword(s):  
Grapevine Virus ◽  
First Report

scholarly journals Detecção do Grapevine virus A e Grapevine virus B por hibridização "dot-blot" com sondas moleculares não radioativas

2005 ◽  
Vol 30 (5) ◽  
pp. 538-542
Author(s):  
Andreia E Moreira ◽  
José O Gaspar ◽  
Hugo Kuniyuki
Keyword(s):  
Grapevine Virus ◽  
Rt Pcr ◽  
Dot Blot ◽  
Grapevine Virus A ◽  
Grapevine Virus B ◽  
Vitis Spp ◽  
Kober Stem Grooving

O vírus A da videira (Grapevine virus A, GVA) e o vírus B da videira (Grapevirus virus B, GVB) estão associados à acanaladura do lenho de Kober ("Kober stem grooving") e ao fendilhamento cortical da videira ("grapevine corky bark"), respectivamente. Este trabalho descreve o uso de sondas moleculares de cDNA na detecção de isolados do GVA (GVA-SP) e do GVB (GVB-C-SP e GVB-I-SP) em videiras (Vitis spp.) e fumo (Nicotiana occidentalis). As sondas marcadas com digoxigenina foram produzidas por RT-PCR utilizando oligonucleotídeos específicos para os genes da proteína capsidial. Os RNA totais foram extraídos de 45 plantas de diversas variedades de videira e de 13 plantas de fumo inoculadas mecanicamente com o GVB. Os RNA extraídos das plantas infetadas, indexadas biologicamente, hibridizaram com as sondas, não se verificando reação com plantas sadias. Para confirmar os resultados de hibridização, foram também feitos testes de RT-PCR. A utilização de hibridização "dot-blot" com sondas de cDNA mostrou-se eficaz na detecção dos vírus com especificidade e sensibilidade, ressaltando-se que, preferencialmente, folhas maduras e ramos dormentes devem ser utilizados nos testes diagnósticos para o GVB e GVA, respectivamente.

Trends in vector-borne transmission efficiency from coinfected hosts: Grapevine leafroll-associated virus-3 and Grapevine virus A

2020 ◽  
Vol 156 (4) ◽  
pp. 1163-1167 ◽  
Author(s):  
G. K. Blaisdell ◽  
S. Zhang ◽  
A. Rowhani ◽  
V. Klaassen ◽  
M. L. Cooper ◽  
...  
Keyword(s):  
Transmission Efficiency ◽  
Grapevine Virus ◽  
Grapevine Virus A ◽  
Vector Borne
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