Comprehensive Transcriptome and Proteome Analysis of Human Embryonic Stem Cells Differentiation to Neural Cells

2012 ◽  
Vol 2012 (1) ◽  
pp. 9
Author(s):  
Ghasem Hosseini Salekdeh
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Proteome Analysis ◽  
Neural Cells

A proteome analysis of conditioned media from human neonatal fibroblasts used in the maintenance of human embryonic stem cells

PROTEOMICS ◽  
2005 ◽  
Vol 5 (4) ◽  
pp. 978-989 ◽  
Author(s):  
Andrew B. J. Prowse ◽  
Leon R. McQuade ◽  
Katherine J. Bryant ◽  
Derek D. Van Dyk ◽  
Bernard E. Tuch ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Proteome Analysis ◽  
Conditioned Media

scholarly journals Comprehensive Gene Expression Analysis of Human Embryonic Stem Cells during Differentiation into Neural Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (7) ◽  
pp. e22856 ◽  
Author(s):  
Ali Fathi ◽  
Maryam Hatami ◽  
Vahid Hajihosseini ◽  
Faranak Fattahi ◽  
Sahar Kiani ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Expression Analysis ◽  
Human Embryonic Stem Cells ◽  
Gene Expression Analysis ◽  
Embryonic Stem ◽  
Neural Cells

Neural Differentiation of Human Embryonic Stem Cells at the Ultrastructural Level

2010 ◽  
Vol 16 (1) ◽  
pp. 80-90 ◽  
Author(s):  
Jennifer L. Mumaw ◽  
Dave Machacek ◽  
John P. Shields ◽  
Mahesh C. Dodla ◽  
Sujoy K. Dhara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Neural Differentiation ◽  
Embryonic Stem ◽  
Ultrastructural Level ◽  
Culture Conditions ◽  
Ultrastructural Changes ◽  
Neural Cells ◽  
Cell Therapies

AbstractNeurodegerative disorders affect millions of people worldwide. Neural cells derived from human embryonic stem cells (hESC) have the potential for cell therapies and/or compound screening for treating affected individuals. While both protein and gene expression indicative of a neural phenotype has been exhibited in these differentiated cells, ultrastuctural studies thus far have been lacking. The objective of this study was to correlate hESC to neural differentiation culture conditions with ultrastructural changes observed in the treated cells. We demonstrate here that in basic culture conditions without growth factors or serum we obtain neural morphology. The addition of brain-derived neurotrophic factor (BDNF) and serum to cultures resulted in more robust neural differentiation. In addition to providing cues such as cell survival or lineage specification, additional factors also altered the intracellular structures and cell morphologies. Even though the addition of BDNF and serum did not increase synaptic formation, altered cellular structures such as abundant polyribosomes and more developed endoplasmic reticulum indicate a potential increase in protein production.

Proteome analysis of human embryonic stem cells organelles

2017 ◽  
Vol 162 ◽  
pp. 108-118 ◽  
Author(s):  
Faezeh Shekari ◽  
Hossein Nezari ◽  
Mehran Rezaei Larijani ◽  
Chia-Li Han ◽  
Hossein Baharvand ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Proteome Analysis
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