Pyruvate Kinase, Glucose 6-phosphate Dehydrogenase and Glutathione Reductase Deficiencies and Neonatal Jaundice in Basrah, Iraq

2003 ◽  
Vol 2003 (2) ◽  
Author(s):  
L. M. Al Naama ◽  
M. K. Hassan ◽  
E. A. Al Saadoon ◽  
T. A. Al Saadoon
Keyword(s):  
Glutathione Reductase ◽  
Pyruvate Kinase ◽  
Neonatal Jaundice ◽  
Glucose 6 Phosphate Dehydrogenase

GLUCOSE-6-PHOSPHATE DEHYDROGENASE AND RED CELL PYRUVATE KINASE DEFICIENCY IN NEONATAL JAUNDICE CASES IN EGYPT

2010 ◽  
Vol 27 (4) ◽  
pp. 262-271 ◽  
Author(s):  
Mohammed Abdel Fattah ◽  
Eman Abdel Ghany ◽  
Alia Adel ◽  
Dalia Mosallam ◽  
Shahira Kamal
Keyword(s):  
Pyruvate Kinase ◽  
Neonatal Jaundice ◽  
Red Cell ◽  
Pyruvate Kinase Deficiency ◽  
Glucose 6 Phosphate Dehydrogenase

scholarly journals The transport of oxidized glutathione from the erythrocytes of various species in the prescence of chromate

1969 ◽  
Vol 114 (4) ◽  
pp. 833-837 ◽  
Author(s):  
Satish K. Srivastava ◽  
Ernest Beutler
Keyword(s):  
Hydrogen Peroxide ◽  
Glutathione Reductase ◽  
Pyruvate Kinase ◽  
Transport Rate ◽  
Oxidized Glutathione ◽  
Phosphogluconate Dehydrogenase ◽  
Presence And Absence ◽  
Glucose 6 Phosphate Dehydrogenase

1. Erythrocytes from normal and glucose 6-phosphate dehydrogenase-deficient humans were subjected to hydrogen peroxide diffusion to oxidize the GSH. Studies were carried out in the presence and absence of chromate to inhibit glutathione reductase and with or without the addition of glucose. 2. The GSH content of erythrocytes from other species was oxidized by subjecting them to hydrogen peroxide diffusion in the presence of chromate and glucose. 3. Chromate (1·3mm) inhibited glutathione reductase by about 80%, whereas glucose 6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, hexokinase, phosphofructokinase and pyruvate kinase were not inhibited. 4. The GSSG formed was transported from the erythrocytes to the medium. 5. The transport rate of GSSG from glucose 6-phosphate dehydrogenase-deficient erythrocytes subjected to hydrogen peroxide diffusion in the presence of chromate was comparable with that from normal and glucose 6-phosphate dehydrogenase-deficient erythrocytes. 6. The rate of transport of GSSG from erythrocytes of various species studied could be ranked: pigeon>rabbit>rat>donkey>man>dog>horse>sheep>chicken>fish.

scholarly journals A Series of New Screening Procedures for Pyruvate Kinase Deficiency, Glucose-6-Phosphate Dehydrogenase Deficiency, and Glutathione Reductase Deficiency

Blood ◽  
1966 ◽  
Vol 28 (6) ◽  
pp. 942-942
Author(s):  
ERNEST BEUTLER ◽  
Agnes Halasz
Keyword(s):  
Glutathione Reductase ◽  
Pyruvate Kinase ◽  
Dehydrogenase Deficiency ◽  
Pyruvate Kinase Deficiency ◽  
Reductase Deficiency ◽  
Screening Procedures ◽  
Glutathione Reductase Deficiency ◽  
Glucose 6 Phosphate Dehydrogenase

Abstract BEUTLER, E.: A SERIES OF NEW SCREENING PROCEDURES FOR PYRUVATE KINASE DEFICIENCY, GLUCOSE-6-PHOSPHATE DEHYDROGENASE DEFICIENCY, AND GLUTATHIONE REDUCTASE DEFICIENCY. Blood 28:553-562, 1966. In the October 1966 issue of Blood, on page 557 the concentration for GSSG was erroneously given as .003 M. The line under B. Reaction Mixture should read: GSSG, .033M 0.1 ml.

scholarly journals AGE-RELATED CHANGES OF GLYCOLYTIC ACTIVITY AND ANTIOXIDANT CAPACITY IN THE BLOOD OF ALLOXAN DIABETIC RATS

2020 ◽  
Vol 20 (3) ◽  
pp. 169-173
Author(s):  
O.Yu. Kushnir ◽  
I.M. Yaremii
Keyword(s):  
Type 1 Diabetes ◽  
Glutathione Reductase ◽  
Pyruvate Kinase ◽  
Reductase Activity ◽  
Diabetic Rats ◽  
Adult Rats ◽  
Age Related ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
The Impact

The increasing incidence of type 1 diabetes coupled with advances in treatment of type 1 diabetes has resulted in an unprecedented number of older adults living with and controllable type 1 diabetes. The objective of this experimental study was to assess the impact of aging on the level of basal glycaemia and activities of glucose-6-phosphate dehydrogenase [EC1.1.1.49], pyruvate kinase [EC 2.7.1.40] and glutathione reductase [EC1.6.4.2] in erythrocytes of alloxan-diabetic rats. Methods: We used 100 male Wistar rats, divided into two age groups: I group included- 2-month (adult) animals, and II group was made up of 4-month (old) animals. Diabetes was modelled by injecting the rats with 5% solution of alloxan monohydrate intraperitoneally in a dose of 170 mg/kg. Blood was taken from the tail vein to evaluate the basal glycaemia on 5-th and 47-th day after the alloxan injection. Rats were sacrificed on the 47-th day of the experiment in accordance with the regulations on ethical treatment of vertebrates. The assessment of the activity of the enzymes was carried out by standard methods. Statistical analysis was performed by using Statistica 10 StatSoft Inc. Results. The level of basal glycaemia on the fifth day of the experiment in the animals of both groups went up on average by 115% from baseline values. We founded that on 47-th day this index was higher in group of old rats by 20% than in adult rats. Pyruvate kinase activity in erythrocytes of adult and old animals with diabetes decreased by 35% and 50% respectively compared with the control. Glucose-6-phosphate dehydrogenase activity in erythrocytes of adult and old animals with diabetes decreased by 27% and 45% respectively compared with the control on 47-th day. The changes may be considered as the result of age-related disorders of glucose metabolism due to disturbances in free radical mechanisms. Glutathione reductase activity in erythrocytes of adult and old animals with diabetes decreased by 29% and 35% respectively compared with the control on 47-th day. Conclusion. We have determined when getting aged, the alloxan-diabetic rats demonstrate changes in the sensitivity of pyruvate kinase, glucose-6-phosphate dehydrogenase and glutathione reductase activities in erythrocytes resulted from the effect of diabetes mellitus factors (hyperglycaemia). We can suggest that glycaemic control is key purpose for older patients with type 1 diabetes in order to prevent of complication, which can be aggravated with age.

scholarly journals A Series of New Screening Procedures for Pyruvate Kinase Deficiency, Glucose-6-Phosphate Dehydrogenase Deficiency, and Glutathione Reductase Deficiency

Blood ◽  
1966 ◽  
Vol 28 (4) ◽  
pp. 553-562 ◽  
Author(s):  
ERNEST BEUTLER ◽  
Agnes Halasz
Keyword(s):  
Glutathione Reductase ◽  
Pyruvate Kinase ◽  
Dehydrogenase Deficiency ◽  
Triosephosphate Isomerase ◽  
General Procedure ◽  
Red Cell ◽  
Pyruvate Kinase Deficiency ◽  
Reductase Deficiency ◽  
Glutathione Reductase Deficiency ◽  
Glucose 6 Phosphate Dehydrogenase

Abstract A new type of screening procedure for the detection of enzymatic defects of the red cell has been described. The blood or red cell sample is added to the reaction mixture. After a suitable period of incubation a drop of the mixture is spotted on filter paper, permitted to dry, and examined for fluorescence under UV light. In this way the oxidation of reduction of pyridine nucleotides is readily evaluated. Reaction mixtures for the detection of glucose-6-phosphate dehydrogenase deficiency, pyruvate kinase deficiency, and glutathione reductase deficiency are described. The same general procedure should be readily adaptable to the detection of other enzymatic deficiencies of red cells, such as phosphogluconate dehydrogenase deficiency or triosephosphate isomerase deficiency.

scholarly journals INFLUENCE OF MELATONIN ON AGE-RELATED CHANGES IN CARBOHYDRATE METABOLISM AND ANTIOXIDANT CAPACITY IN THE BLOOD OF ALLOXAN DIABETIC RATS

2021 ◽  
Vol 21 (3) ◽  
pp. 228-233
Author(s):  
I.M. Yaremii ◽  
O.Yu. Kushnir
Keyword(s):  
Glutathione Reductase ◽  
Pyruvate Kinase ◽  
Reductase Activity ◽  
Direct Action ◽  
Age Groups ◽  
Diabetic Rats ◽  
Control Group ◽  
Adult Rats ◽  
Age Related ◽  
Glucose 6 Phosphate Dehydrogenase

Melatonin and its metabolites have potent antioxidant/anti-inflammatory properties, and they have proven to be highly effective in a variety of disorders linked to inflammation and oxidative stress. The object of this experimental research was to ascertain the influence of aging on the level of basal glycemia and activities of glucose-6-phosphate dehydrogenase [EC1.1.1.49], pyruvate kinase [EC 2.7.1.40] and glutathione reductase [EC1.6.4.2] in erythrocytes of alloxan diabetic rats on the background of melatonin injections. Methods: We used 100 male Wistar rats, two age groups: the - 2-month (adult), and II - 4-month (old). Alloxan diabetes was evoked via injecting the rats with a 5% solution of alloxan monohydrate intraperitoneally in a dose of 170 mg/kg. Four days after diabetes induction, rats were divided into diabetic (untreated) and melatonin-diabetic group (10 mg/kg, daily and intraperitoneally for six weeks). Blood was taken from the tail vein evaluate the basal glycemia on 5-th and 47-th day after the injection of alloxan. Rats were sacrificed at the 47-th day of the experiment accordance with the ethical treatment of animals. Determinations of the enzymes activities were by standard methods. Statistical analysis was performed using Statistica 10 StatSoft Inc. Results. The level of basal glycemia on the fifth day of the experiment in animals of both groups increased on average by 115% from baseline values. We founded that on 47-th day this index was higher in group of old rats on 20% more than in adult rats. Pyruvate kinase activity in erythrocytes of adult and old animals with diabetes decreased by 34% and 51% respectively compared with the control. glucose-6-phosphate dehydrogenase activity in erythrocytes of adult and old animals with diabetes decreased by 25% and 44% respectively compared with the control on 47-th day. The changes may be the result of age-related disorders of glucose metabolism due to disturbances in free radical mechanisms. Glutathione reductase activity in erythrocytes of adult and old animals with diabetes decreased by 30% and 36% respectively compared with the control on 47-th day. A 42-days injection of melatonin to the alloxan diabetic rats of both groups contributed to a normalization of the level of basal glycemia, the activities of pyruvate kinase and glutathione reductase in the rat blood, as well as to a considerable increase of the activity of glucose-6-phosphate dehydrogenase, whose level exceeded by average 9% this particular index in the control group of animals. Under the influence of melatonin increase activity of glucose-6-phosphate dehydrogenase in the blood of rats may be due to the increasing number of substrate for glucose-6-phosphate dehydrogenase (stimulating the flow of glucose into cells and its phosphorylation) and direct action. Conclusion. In this case melatonin probably increases use of glucose for regeneration of NADPH2 and aerobic oxidation of glucose that indicate an acceleration of antioxidative protection and energy production in blood of adult and old diabetic rats.

scholarly journals Isoniazid acetylating phenotype in patients with paracoccidioidomycosis and its relationship with serum sulfadoxin levels, glucose-6-phosphate dehydrogenase and glutathione reductase activities

1991 ◽  
Vol 24 (2) ◽  
pp. 111-114 ◽  
Author(s):  
Benedito Barraviera ◽  
Paulo Câmara Marques Pereira ◽  
Jussara Marcondes Machado ◽  
Maria Julia de Souza ◽  
Carlos Roberto G. Lima ◽  
...  
Keyword(s):  
Glutathione Reductase ◽  
Reductase Activity ◽  
Serum Levels ◽  
Slow Acetylators ◽  
Slow Acetylator ◽  
A Value ◽  
Acti Vity ◽  
Glutathione Reductase Activity ◽  
Glutathione Reductase Deficiency ◽  
Glucose 6 Phosphate Dehydrogenase

The authors evaluated the isoniazid acetylating phenotype and measured hematocrit, hemoglobin, glucose-6-phosphate dehydrogenase and glutathione reductase activities plus serum sulfadoxin levels in 39 patients with paracoccidioidomycosis (33 males and 6 females) aged 17 to 58 years. Twenty one (53.84%) of the patients presented a slow acetylatingphenotype and 18(46.16%) a fast acetylating phenotype. Glucose-6-phosphate- dehydrogenase (G6PD) acti vity was decreased in 5(23.80%) slow acetylators and in 4(22.22%) fast acetylators. Glutathione reductase activity was decreased in 14 (66.66%) slow acetylators and in 12 (66.66%) fast acetylators. Serum levels of free and total sulfadoxin Were higher in slow acetylator (p < 0.02). Analysis of the resultspermitted us to conclude that serum sulfadoxin levels are related to the acetylatorphenotype. Furthermore, sulfadoxin levels were always above 50 µg/ml, a value considered therapeutic. Glutathione reductase deficiency observed in 66% of patients may be related to the intestinal malabsorption of nutrients, among them riboflavin, a FAD precursor vitamin, inpatients with paracoceidioidomycosis.

Sign in / Sign up

Export Citation Format

Share Document