Detection of PER-1 Type Extended Spectrum Beta Lactamase in the Acinetobacter baumannii Species Isolated from Blood Cultures and Investigation of Clonal Relationship

2013 ◽  
Vol 33 (2) ◽  
pp. 389-395 ◽  
Author(s):  
Mediha COŞAR ◽  
Emine İnci TUNCER ◽  
Uğur ARSLAN ◽  
Ahmet MANSUR ◽  
Barış OTLU ◽  
...  
Keyword(s):  
Acinetobacter Baumannii ◽  
Blood Cultures ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
Clonal Relationship ◽  
Extended Spectrum

scholarly journals COMPARATIVE STUDY OF GELATINASE ACTIVITY AND PELLICLE FORMATION AMONG EXTENDED-SPECTRUM BETA-LACTAMASE AND NON-EXTENDED-SPECTRUM BETA-LACTAMASE PRODUCING ACINETOBACTER BAUMANNII FROM DIABETIC FOOT ULCER INFECTIONS

2018 ◽  
Vol 11 (9) ◽  
pp. 496 ◽  
Author(s):  
Diwan Mahmood Khan ◽  
I. Venkatakrishna Rao ◽  
M. S. Moosabba
Keyword(s):  
Acinetobacter Baumannii ◽  
Foot Ulcer ◽  
Diabetic Foot Ulcer ◽  
Beta Lactamase ◽  
Esbl Producer ◽  
Pellicle Formation ◽  
Gelatinase Activity ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Esbl Producers

Objective: The aim of the study was to assess and compare the gelatinase activity and pellicle formation in extended-spectrum beta-lactamase (ESBL) and non-ESBL producing Acinetobacter baumannii isolates from diabetic foot ulcer infection (DFI).Methods: A total of 42 isolates of A. baumannii recovered from patients of DFI from September 2016 to February 2018. Isolates were identified by the standard microbiological method and confirmed by the BD Phoenix 100 system. The antimicrobial susceptibility test was performed by the Kirby–Bauer disk diffusion method and ESBL was detected by double disk diffusion synergy test method. Gelatinase production was determined by the Luria Bertani agar supplemented with 30 g/L gelatin, and pellicle formation was determined by the Mueller-Hinton broth which is incubated at two different temperatures.Results: A total of 42 A. baumannii isolates were multidrug resistant. Among 21 isolates, each was ESBL and non-ESBL producers. Pellicle formation at 25°C in ESBL and non-ESBL producer isolates was 47.61% (10/21) and 28.57% (06/21). Pellicle formation at 37°C in ESBL and non-ESBL producer isolates was 57.14% (12/21) and 42.85% (09/21), respectively. Gelatinase production was present in 38.09% ESBL and 28.57% in non-ESBL producers. ESBL strains were more virulent compared to non-ESBL producers among patients of DFIs.Conclusion: This study showed that pellicle formation at 37°C was highly virulent due to ESBL producers. Gelatinase production was elevated in ESBL compared to non-ESBL producer isolates. This attribute of the isolates could render ESBL positive more pathogenic. Colistin and polymyxin B are the only choices of treatment for multidrug-resistant Acinetobacter baumannii infections.

Prevalence of extended-spectrum beta-lactamase-producing Enterobacteriaceae isolated from blood cultures in Africa

2015 ◽  
Vol 45 (9) ◽  
pp. 374-382 ◽  
Author(s):  
S.A. Sangare ◽  
A.I. Maiga ◽  
I. Guindo ◽  
A. Maiga ◽  
N. Camara ◽  
...  
Keyword(s):  
Blood Cultures ◽  
Beta Lactamase ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum

Phenotypic detection of extended-spectrum beta-lactamase in multidrug-resistant acinetobacter baumannii isolated in Fauji Foundation Hospital Rawalpindi

2021 ◽  
pp. 1-12
Author(s):  
Asim Ali Shah ◽  
Yasir Ali ◽  
Ayesha Maqbool ◽  
Shahid Ahmad Abbasi ◽  
Admin
Keyword(s):  
Acinetobacter Baumannii ◽  
Sampling Technique ◽  
Polymyxin B ◽  
Multidrug Resistant ◽  
Cross Sectional Study ◽  
Beta Lactamase ◽  
Cross Sectional ◽  
Extended Spectrum Beta Lactamase ◽  
Extended Spectrum ◽  
Multiple Drugs

Abstract Objective: To evaluate the phenotypic detection of extended-spectrum beta-lactamase in multidrug-resistant acinetobacter baumannii. Methods: The cross-sectional study was conducted at the Department of Microbiology, Fauji Foundation Hospital, Rawalpindi, Pakistan, from August 2018 to April 2019, after the ethical approval from the Institutional Review Committee. Consecutive Non- probability sampling technique was used, and comprised clinical specimens, including pus, blood, sputum, urine, tracheal tubes and canula double lumen, which were processed using standard protocols. Colonies of acinetobacter baumannii were identified by gram staining and Analytical Profile Index-20E kit. Combination disc method was used for the identification of extended-spectrum beta-lactamse. Clinical and Laboratory Standards Institute guidelines were used for antimicrobial susceptibility. Data was analysed using SPSS 22 and Sample size was calculated by using earlier study with 5 % margin of error and 95 % confidence level. Results: Of the 78 isolates, 58(74.4%) related to females and 20(25.6%) to males. There was no extended-spectrum beta-lactamse producer. Imipenem, meropenem, cefotaxime, ampicillin and ceftazidime showed 100% resistance, while colistin and polymyxin B were sensitive to all strains. The incidence rate was high in samples isolated from tracheal tubes 47(60.3%), followed by pus 21(26.9%). Age was not found to be a significant factor (p>0.05).   Conclusion: Acinetobacter baumannii showed a high resistance to multiple drugs and was not confined to any specific age group. Colistin and polymyxin B were found to be better choices. Continuous...

Sign in / Sign up

Export Citation Format

Share Document