scholarly journals Pulmonary interstitial oedema

2019 ◽  
Author(s):  
Daniel Bell ◽  
Balint Botz
Keyword(s):  
Interstitial Oedema

Interstitial oedema in patients undergoing cardiac surgery: Evaluation through lung ultrasonography

2018 ◽  
Vol 32 ◽  
pp. S49-S50
Author(s):  
A. Sengel ◽  
Tulun Ozturk ◽  
D. Amanvermez Senarslan ◽  
F. Nizamoglu Yıldırım ◽  
A.M. Kececi
Keyword(s):  
Cardiac Surgery ◽  
Lung Ultrasonography ◽  
Interstitial Oedema

PREOVULATORY CHANGES IN THE OVARIES DURING THE FIRST SPONTANEOUS PRO-OESTRUS IN THE RAT

1975 ◽  
Vol 67 (2) ◽  
pp. 259-265 ◽  
Author(s):  
P. OSMAN
Keyword(s):  
Adult Animal ◽  
Cumulus Cells ◽  
Follicular Atresia ◽  
Vaginal Smears ◽  
Pronounced Increase ◽  
Causal Factors ◽  
Ovarian Weight ◽  
Interstitial Oedema ◽  
Adult Rat ◽  
New Crop

SUMMARY Events in the ovaries during the first spontaneous pro-oestrus were compared with those in the adult rat. The occurrence of first spontaneous pro-oestrus was determined from vaginal smears after surgical opening of the vagina. Pubertal rats showed a remarkable increase in ovarian weight from 10.00 to 15.00 h on the day of pro-oestrus, which was absent in the adults. They also showed an earlier and more pronounced interstitial oedema. Dispersion of cumulus cells, resumption of meiosis and ovulation also occurred slightly earlier in pubertal rats. A distinct difference was found in the growth rate of follicles of ovulatory size, i.e. follicles of ≥ 500 × 105 μm3. At the first pro-oestrus these follicles showed a slower and less pronounced increase in size from 10.00 to 17.00 h compared with the adult follicles at this time, although this disparity was made up by a marked increase in growth from 17.00 to 24.00 h. The development of a new crop of follicles progressed similarly in the pubertal and adult animals. Follicular atresia seemed to progress more rapidly from 10.00 to 15.00 h in the pubertal animals compared with adults but from 15.00 h onwards no further differences were observed. The possible causal factors underlying the observed differences between the pubertal and adult animal are discussed.

Autopsy Findings in Turtle Flesh Poisoning

1988 ◽  
Vol 28 (2) ◽  
pp. 142-144 ◽  
Author(s):  
N. Chandrasiri ◽  
P. L. Ariyananda ◽  
S. S. D. Fernando
Keyword(s):  
Sri Lanka ◽  
Toxic Substance ◽  
Eretmochelys Imbricata ◽  
Younger Child ◽  
Interstitial Oedema ◽  
Autopsy Findings ◽  
Alveolar Oedema ◽  
The Brain ◽  
Alveolar Septa ◽  
Breast Fed

There is a paucity of literature in Sri Lanka and internationally on autopsy findings of turtle flesh poisoning. Of the five varieties of endemic sea turtles in Sri Lanka, only the Hawksbill type (Eretmochelys imbricata – locally termed Pothu Kesbawa, Leli Kesbawa, Pana Kesbawa) is poisonous. Of the two cases described one is a breast-fed child of age 1 year 3 months and the other of 13 years. The younger child did not eat the flesh but was breast fed by his mother before becoming ill, but after she had eaten the flesh. The main findings are in the lungs. There is gross thickening of the alveolar septa with interstitial oedema with haemorrhage and micro-thrombi in the lumen of pulmonary arterioles. There is a remarkable lack of intra-alveolar oedema. The brain showed interstitial oedema. Myocardial fibres showed diffuse degeneration, necrosis and interstitial oedema with an acellular reaction. The toxic substance as postulated may be a component of a variety of algae present only in certain seasons.

P–452 The challenge of ovarian tissue culture: 2D versus 3D

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
A T Almeid. Santos ◽  
A S Pais ◽  
S Reis ◽  
M Laranjo ◽  
F Caramelo ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Ovarian Tissue ◽  
3D Culture ◽  
Endocrine Function ◽  
Cell Degeneration ◽  
Interstitial Oedema ◽  
Conventional Culture ◽  
Histological Score ◽  
Alginate Scaffold ◽  
Alginate Matrix

Abstract Study question Does an alginate matrix scaffold improve ovarian tissue culture? Summary answer Ovarian tissue culture within an alginate scaffold has no advantage over conventional culture, being more time consuming and less reproductible What is known already Cryopreservation of ovarian tissue is a powerful technique for preserving female fertility, as it can restore fertility and endocrine function. Several studies have been carried out aiming to increase the longevity of the transplant and decrease the risk of reimplantation of neoplastic cells. For in vitro follicle culture, recent research has shifted from two dimensional (2D) toward the use of three-dimensional (3D) structures. The use of a matrix maintains the architecture and mimics in vivo conditions, with a variable access to oxygen and nutrients. This bridges the gap between conventional cell culture and animal models. Study design, size, duration Ovarian tissue fragments were divided into 2 groups: conventional culture (2D culture) and culture using an alginate matrix scaffold (3D culture). Tissue was evaluated at four time-points: immediately after thawing and after 24, 48 and 72 hours of culture. Participants/materials, setting, methods Rat ovarian tissue was cryopreserved and thawed with validated protocols. Follicular analysis was conducted after haematoxylin and eosin staining, regarding density, classification and degeneration. Tissue viability was assessed using lactate dehydrogenase (LDH) levels in supernatants and histological score. Three parameters were considered, namely, interstitial oedema, follicular cell degeneration and percentage of tissue in necrosis. Apoptosis was assessed by caspase 3 immunostaining. Proliferating cells were identified using Ki67 immunohistochemical labelling. Main results and the role of chance Follicular density, cell proliferation and apoptosis both in follicles and stroma was similar in both culture conditions. Stromal cells proliferation was stable in conventional culture but decreased in 3D culture (p = 0.001), which can be explained by the rigidity of alginate matrix. At 24 hours of culture, cytotoxicity was lower in the 3D model (p = 0.006), due to low levels of LDH in the supernatant, that may be related to retention within the matrix. As culture time increased cytotoxicity seemed to be similar. Degradation of the tissue was suggested by the histological score analysis of tissue during 72 hours of culture. Tissue injury was greater (p = 0.01) in 3D culture due to higher interstitial oedema (p = 0.017) and tissue necrosis (p = 0.035). In the interior of the alginate scaffold, the bioavailability of oxygen and nutrients may be limited, affecting cell survival over time and conditioning higher level of necrosis and release of intracellular content. Limitations, reasons for caution There are two major limitations that should be addressed in future research, namely the study of the tissue-matrix interactions and culture medium supplementation to decrease follicular atresia. Wider implications of the findings: There is no advantage in the use of an alginate matrix scaffold for ovarian tissue culture, as it is more time consuming, difficult to perform and less reproductible. Trial registration number Not applicable

scholarly journals Experimental isolation and preservation of solid organs before transplantation: effects of pretreatment using four different molecules

2020 ◽  
Vol 48 (6) ◽  
pp. 030006052093345
Author(s):  
Maria Chalasti ◽  
Christos Iordanou ◽  
Zisis Kratiras ◽  
Aikaterini Stylianaki ◽  
Eleni-Andriana Trigka ◽  
...  
Keyword(s):  
Control Group ◽  
Average Weight ◽  
Large White ◽  
Interstitial Oedema ◽  
Post Harvest ◽  
Transplantation Surgery ◽  
Ischaemia Reperfusion ◽  
Liver And Pancreas ◽  
Potential Failure ◽  
Positive Effect

Objectives In transplantation surgery, the ischaemic organ and reperfusion impairment after cold storage remains a considerable risk factor for impaired function and potential failure of the grafted organ. Substantial logistical efforts have been undertaken to reduce the cold ischaemic time because the demand for available transplant organs and the periods of cold ischaemia are increasing. Methods Four molecules were investigated (erythropoietin, sildenafil, lazaroid [U74389G], octreotide) in individual intravenous infusions 1 hour before the organ was harvested. This study was performed in 30 healthy landrace/large-white pigs (male; >10 weeks old; average weight, 22 ± 2 kg) in groups of six. The organs were studied at harvest, and at 8 and 24 hours post-harvest. Results The lazaroid molecule increased malondialdehyde (MDA) levels in the liver and pancreas at 8 hours. Hepatic lazaroid molecules improved liver histology at 8 and 24 hours. For kidneys, erythropoietin had a positive effect at 24 hours post-harvest. For the pancreas, octreotide showed better performance. In the lungs, there was less interstitial oedema with erythropoietin and lazaroid compared with the control group at 8 hours post-harvest. Conclusion All molecules had a positive effect and decreased ischaemia/reperfusion graft injury. Thus, pretreatment before organ harvest has a beneficial role.

scholarly journals Myocardial microvascular permeability, interstitial oedema, and compromised cardiac function

2010 ◽  
Vol 87 (2) ◽  
pp. 331-339 ◽  
Author(s):  
Ranjeet M. Dongaonkar ◽  
Randolph H. Stewart ◽  
Hans J. Geissler ◽  
Glen A. Laine
Keyword(s):  
Cardiac Function ◽  
Microvascular Permeability ◽  
Interstitial Oedema

scholarly journals Acute and severe haemorrhage following pentosan polysulfate injection in a Cornish Rex

2021 ◽  
Vol 7 (2) ◽  
pp. 205511692110586
Author(s):  
Miranda X Tong ◽  
Jessica F Romine ◽  
Michael R Hardcastle
Keyword(s):  
Large Scale ◽  
Severe Anaemia ◽  
Off Label Use ◽  
Pentosan Polysulfate ◽  
Interstitial Oedema ◽  
Off Label ◽  
Severe Haemorrhage ◽  
Case Summary ◽  
The Hours ◽  
Post Mortem Findings

Case summary A 14-year-old male castrated Cornish Rex cat was referred for lethargy progressing rapidly to collapse in the hours following a subcutaneous injection of a product containing 100 mg/ml pentosan polysulfate sodium and 168 mg/ml glucosamine. Physical examination revealed the cat to be in hypotensive shock with swelling and interstitial oedema around the cranial thorax and caudal cervical regions without cutaneous haemorrhage. Initial diagnostics revealed a severe anaemia (packed cell volume 11%) which later deteriorated further, necessitating a blood transfusion and aggressive fluid therapy. Post-transfusion, the patient remained dyspnoeic and subsequent diagnostics found evidence of pre-existing cardiomyopathy and congestive heart failure. The cat was euthanased 24 h following presentation due to increasing dyspnoea. Post-mortem findings were of severe subcutaneous and intermuscular haemorrhage over the neck and thorax, among other changes. There were no detectable levels of coumarin anticoagulants in the liver. Relevance and novel information This is the first reported case of acute subcutaneous and intermuscular haemorrhage of this severity suspected to be related to the off-label use of an injectable product containing pentosan polysulfate in a cat. Given the popularity of its use for feline arthritis, there is a need for large-scale clinical trials to evaluate the safety and efficacy of products containing pentosan polysulfate for cats, and any side effects to be reported.

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