scholarly journals Liver sinusoid

2018 ◽  
Author(s):  
Daniel Bell ◽  
Maxime St-Amant
Keyword(s):  
Liver Sinusoid

scholarly journals Monocyte-induced recovery of inflammation-associated hepatocellular dysfunction in a biochip-based human liver model

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Marko Gröger ◽  
Knut Rennert ◽  
Benjamin Giszas ◽  
Elisabeth Weiß ◽  
Julia Dinger ◽  
...  
Keyword(s):  
Liver Dysfunction ◽  
Human Liver ◽  
Immune Cell ◽  
Early Event ◽  
Liver Sinusoid ◽  
Human Sepsis ◽  
Cellular Events ◽  
Cell Layers ◽  
Parenchymal Cells

Abstract Liver dysfunction is an early event in sepsis-related multi-organ failure. We here report the establishment and characterization of a microfluidically supported in vitro organoid model of the human liver sinusoid. The liver organoid is composed of vascular and hepatocyte cell layers integrating non-parenchymal cells closely reflecting tissue architecture and enables physiological cross-communication in a bio-inspired fashion. Inflammation-associated liver dysfunction was mimicked by stimulation with various agonists of toll-like receptors. TLR-stimulation induced the release of pro- and anti-inflammatory cytokines and diminished expression of endothelial VE-cadherin, hepatic MRP-2 transporter and apolipoprotein B (ApoB), resulting in an inflammation-related endothelial barrier disruption and hepatocellular dysfunction in the liver organoid. However, interaction of the liver organoid with human monocytes attenuated inflammation-related cell responses and restored MRP-2 transporter activity, ApoB expression and albumin/urea production. The cellular events observed in the liver organoid closely resembled pathophysiological responses in the well-established sepsis model of peritoneal contamination and infection (PCI) in mice and clinical observations in human sepsis. We therefore conclude that this human liver organoid model is a valuable tool to investigate sepsis-related liver dysfunction and subsequent immune cell-related tissue repair/remodeling processes.

scholarly journals Surface charge distribution on the endothelial cell of liver sinusoids.

1984 ◽  
Vol 99 (2) ◽  
pp. 639-647 ◽  
Author(s):  
L Ghitescu ◽  
A Fixman
Keyword(s):  
Endothelial Cell ◽  
Cell Surface ◽  
Liver Sinusoid ◽  
Electron Microscopic ◽  
Coated Pits ◽  
Endothelial Cell Surface ◽  
Liver Sinusoids ◽  
Surface Charge Distribution ◽  
Cationic Residues

The topography of the charged residues on the endothelial cell surface of liver sinusoid capillaries was investigated by using electron microscopic tracers of different size and charge. The tracers used were native ferritin (pl 4.2-4.7) and its cationized (pl 8.4) and anionized (pl 3.7) derivatives, BSA coupled to colloidal gold (pl of the complex 5.1), hemeundecapeptide (pl 4.85), and alcian blue (pl greater than 10). The tracers were either injected in vivo or perfused in situ through the portal vein of the mouse liver. In some experiments, two tracers of opposite charge were sequentially perfused with extensive washing in between. The liver was processed for electron microscopy and the binding pattern of the injected markers was recorded. The electrostatic nature of the tracer binding was assessed by perfusion with high ionic strength solutions, by aldehyde quenching of the plasma membrane basic residues, and by substituting the cell surface acidic moieties with positively charged groups. Results indicate that the endothelial cells of the liver sinusoids expose on their surface both cationic and anionic residues. The density distribution of these charged groups on the cell surface is different. While the negative charge is randomly and patchily scattered all over the membrane, the cationic residues seem to be accumulated in coated pits. The charged groups co-exist in the same coated pit and bind the opposite charged macromolecule. It appears that the fixed positive and negative charges of the coated pit glycocalyx are mainly segregated in space. The layer of basic residues is located at 20-30-nm distance of the membrane, while most of the negative charges lie close to the external leaflet of the plasmalemma.

Dose- and Time-Dependent Effects of Ethanol on Functional and Structural Aspects of the Liver Sinusoid in the Mouse

1997 ◽  
Vol 21 (6) ◽  
pp. 1128-1136 ◽  
Author(s):  
G. Sarphie ◽  
N. B. D'Souza ◽  
D. H. Thiel ◽  
D. Hill ◽  
C. J. McClain ◽  
...  
Keyword(s):  
Time Dependent ◽  
Liver Sinusoid ◽  
Structural Aspects

Ultrastructure of the Liver Sinusoid

2014 ◽  
pp. 1235-1240
Author(s):  
David G. Le Couteur ◽  
Alessandra Warren ◽  
Victoria Cogger ◽  
Patrick Bertolino
Keyword(s):  
Liver Sinusoid

The Role of Endotheliocytes of Liver Sinusoid Capillaries in the Pathogenesis of Disorders in the Vascular-Platelet Link of Hemostasis in the Acute Period of Traumatic Brain Injury

2020 ◽  
Vol 14 (1) ◽  
pp. 43-51
Author(s):  
G. A. Boyarinov ◽  
Ye. I. Yakovleva ◽  
A. V. Deryugina ◽  
O. D. Solovyeva ◽  
L. V. Boyarinova ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Liver Sinusoid ◽  
Acute Period

scholarly journals HepaChip-MP – a twenty-four chamber microplate for a continuously perfused liver coculture model

Lab on a Chip ◽  
2020 ◽  
Vol 20 (16) ◽  
pp. 2911-2926
Author(s):  
Marius Busche ◽  
Olena Tomilova ◽  
Julia Schütte ◽  
Simon Werner ◽  
Meike Beer ◽  
...  
Keyword(s):  
Perfused Liver ◽  
Culture Chamber ◽  
Liver Sinusoid ◽  
Coculture Model

HepaChip-MP: a 24-culture-chamber, automated microfluidic in vitro model of the liver sinusoid in multiwellplate format.

The scavenger endothelial cell: a new player in homeostasis and immunity

2012 ◽  
Vol 303 (12) ◽  
pp. R1217-R1230 ◽  
Author(s):  
Karen Kristine Sørensen ◽  
Peter McCourt ◽  
Trond Berg ◽  
Clive Crossley ◽  
David Le Couteur ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Critical Role ◽  
Biological Macromolecules ◽  
Cell Type ◽  
Liver Sinusoid ◽  
Fc Gamma Receptor ◽  
Terrestrial Vertebrates ◽  
Gamma Receptor ◽  
Endocytic Machinery ◽  
Efficient Uptake

To maintain homeostasis, the animal body is equipped with a powerful system to remove circulating waste. 1 This review presents evidence that the scavenger endothelial cell (SEC) is responsible for the clearance of blood-borne waste macromolecules in vertebrates. SECs express pattern-recognition endocytosis receptors (mannose and scavenger receptors), and in mammals, the endocytic Fc gamma-receptor IIb2. This cell type has an endocytic machinery capable of super-efficient uptake and degradation of physiological and foreign waste material, including all major classes of biological macromolecules. In terrestrial vertebrates, most SECs line the wall of the liver sinusoid. In phylogenetically older vertebrates, SECs reside instead in heart, kidney, or gills. SECs, thus, by virtue of their efficient nonphagocytic elimination of physiological and microbial substances, play a critical role in the innate immunity of vertebrates. In major invertebrate phyla, including insects, the same function is carried out by nephrocytes. The concept of a dual-cell principle of waste clearance is introduced to emphasize that professional phagocytes (macrophages in vertebrates; hemocytes in invertebrates) eliminate larger particles (>0.5 μm) by phagocytosis, whereas soluble macromolecules and smaller particles are eliminated efficiently and preferentially by clathrin-mediated endocytosis in nonphagocytic SECs in vertebrates or nephrocytes in invertebrates. Including these cells as important players in immunology and physiology provides an additional basis for understanding host defense and tissue homeostasis.

scholarly journals Human Liver Sinusoid on a Chip for Hepatitis B Virus Replication Study

Micromachines ◽  
2017 ◽  
Vol 8 (1) ◽  
pp. 27 ◽  
Author(s):  
Young Kang ◽  
Siddhartha Rawat ◽  
Nicholas Duchemin ◽  
Michael Bouchard ◽  
Moses Noh
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Virus Replication ◽  
Human Liver ◽  
Replication Study ◽  
Liver Sinusoid ◽  
B Virus

398 Study of the effects of maternal administration of morphine on the embryonic liver sinusoid and kupffer cells

2003 ◽  
Vol 144 ◽  
pp. s108
Author(s):  
Gh. Kaka ◽  
H. Sahraei ◽  
S.H. Sadraei ◽  
H. Bahadoran ◽  
H. Dashtnavard
Keyword(s):  
Kupffer Cells ◽  
Embryonic Liver ◽  
Liver Sinusoid ◽  
Maternal Administration
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