Biochemical Analysis of Neoplastic Versus Nonneoplastic Abdominal Effusions in Dogs

2004 ◽  
Vol 40 (5) ◽  
pp. 372-375 ◽  
Author(s):  
Derek D. Nestor ◽  
Sheila M. McCullough ◽  
David J. Schaeffer
Keyword(s):  
Prospective Study ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Lower Glucose ◽  
Low Glucose ◽  
Malignant Effusions ◽  
Lactate Levels ◽  
High Lactate

This prospective study of 15 dogs evaluated biochemical parameters in abdominal effusions of neoplastic and nonneoplastic origin in an attempt to identify markers for malignant effusions. Dogs in the neoplastic group had statistically significant lower glucose concentrations (mean, 72.6 mg/dL versus 110.0 mg/dL; P=0.0431) and higher lactate levels (mean, 3.81 mmol/L versus 1.68 mmol/L; P=0.0377) in their abdominal fluid than did dogs in the nonneoplastic group, indicating that low glucose and high lactate in abdominal effusions may be markers for neoplasia.

scholarly journals Biomarkers in routine diagnosis of pleural effusions

2018 ◽  
Vol 87 (1-2) ◽  
Author(s):  
Tiva Nemanič ◽  
Aleš Rozman ◽  
Katja Adamič ◽  
Mateja Marc Malovrh
Keyword(s):  
Pleural Fluid ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Pleural Effusions ◽  
Routine Diagnostics ◽  
Routine Diagnosis ◽  
Malignant Effusions ◽  
One Year ◽  
The University ◽  
Aetiological Spectrum

Background: Pleural fluid biochemical analysis is the first step in pleural effusion (PE) diagnostics. Our purpose was to analyse the utility of the biomarkers used at our clinic in the routine diagnosis of PE.Methods: We retrospectively reviewed the PE levels of proteins, lactate dehydrogenase (LDH), alpha amylase (AA), pH and glucose in 433 patients who were treated at the University Clinic Golnik in a one-year period and compared these values with the final identified aetiology of the effusions.Results: The majority of the effusions were determined to be a consequence of malignancy (n = 154) or infection (n = 108). In 94 cases the aetiology of the effusions was heart failure and in 54 cases other diseases, while 23 effusions remained aetiologically undetermined. Considering Light’s criteria, the vast majority of the effusions were correctly classified as exudates or transudates (97.1 %). Comparing paramalignant and malignant effusions, we detected significantly lower values of pleural fluid LDH (p < 0.0005) and proteins (p < 0.0005), and higher pH (p < 0.0005) values in the paramalignant effusions.Conclusion: We have found that pleural LDH and proteins are the most helpful biochemical parameters in our routine diagnosis of pleural effusions and helped us to correctly narrow the aetiological spectrum. Furthermore, significantly higher pleural LDH and protein values and a pH below 7.32 additionally facilitated distinguishing between malignant and paramalignant effusions. Parameters such as glucose and AA are useful in selected cases and have a limited role in routine diagnostics.

Influence of exercise and fasting on blood parameters in juvenile green turtles (Chelonia mydas): implications for health assessments

2020 ◽  
pp. 1-8
Author(s):  
D. March ◽  
E. Ariel ◽  
D. Blyde ◽  
L. Christidis ◽  
B.P. Kelaher
Keyword(s):  
Biochemical Parameters ◽  
Health Assessment ◽  
Chelonia Mydas ◽  
Blood Parameters ◽  
Fasting State ◽  
Intense Exercise ◽  
Post Exercise ◽  
Green Turtles ◽  
Health Assessments ◽  
Lactate Levels

This study investigated the influence of exercise and fasting state on haematologic and biochemical parameters in juvenile green turtles (Chelonia mydas). Animals were divided into two groups; one group was fasted for 72 h and one group was fed 1 h prior to exercise. Exercise was induced by repeated righting reflexes and blood values were measured prior to and post-exercise. Prior to exercise, fasted animals showed significantly decreased levels of urea, pH, PVCO2 and HCO3- and significant increases in Cl- and PVO2, compared to fed animals and fasted animals had significantly poorer exercise performance. Following exercise both fasted and fed animals had significant increases in Na+, K+, Cl-, PVCO2, PVO2, urea and lactate and significant decreases in pH and HCO3-. The magnitude of increase in lactate levels was significantly less in fasted animals. Prior to exercise, a significant correlation was calculated in fasted animals between pH and HCO3-. Following exercise, significant correlations were calculated in fed animals between pH and HCO3-, PVCO2 and lactate, and between pH and HCO3- in fasted animals. These results show that analytical method, fasting state and the physiologic changes induced during the intense exercise can affect haematologic and biochemical analytes and these factors should be considered when interpreting results from health assessment of wild animals.

Persistent high lactate level as a sensitive MR spectroscopy indicator of completed infarction

1990 ◽  
Vol 72 (5) ◽  
pp. 763-766 ◽  
Author(s):  
Kiyohiro Houkin ◽  
Ingrid L. Kwee ◽  
Tsutomu Nakada
Keyword(s):  
Magnetic Resonance ◽  
Cerebral Infarction ◽  
Mr Spectroscopy ◽  
The Other ◽  
Proton Spectroscopy ◽  
Normal Pattern ◽  
Content Type ◽  
Lactate Levels ◽  
High Lactate ◽  
Fine Print

✓ Serial proton (1H) and phosphorus-31 (31P) magnetic resonance (MR) spectroscopy of cerebral infarction was performed in rats to assess the sensitivity of these techniques for use in clinical cerebral infarction. In this experimental chronic infarction model, 31P spectroscopy tended to return to a “normal” pattern within 24 hours after induction of infarction in spite of pathologically proven completed infarction and, therefore, appeared not to be sensitive enough for clinical application. On the other hand, proton spectroscopy invariably showed persistent high lactate levels and was capable of distinguishing completed infarction from reperfused recovered brain. Persistent high lactate levels appear to be a good MR spectroscopic indicator of completed infarction.

scholarly journals The effects of different anticoagulants on biochemical parameters in blood of dairy cows

2015 ◽  
Vol 69 (1-2) ◽  
pp. 13-20
Author(s):  
Branislava Belic ◽  
Marko Cincovic ◽  
Maja Dosenovic ◽  
Dragica Stojanovic ◽  
Zorana Kovacevic
Keyword(s):  
Alkaline Phosphatase ◽  
Dairy Cows ◽  
Alkaline Phosphatase Activity ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Blood Parameters ◽  
Albumin Level ◽  
Blood Samples ◽  
Holstein Friesian

Routine analysis of blood parameters requires high precision. Therefore a significant number of methods and recommendations has been developed to ensure the obtaining of precise results. The aim of this study was to investigate whether the type of anticoagulant affects the values of biochemical parameters in the blood of dairy cows in relation to the values obtained from the serum. The study was carried out on 20 healthy cows of Holstein- Friesian breed in the second month of lactation. The blood was taken by venipuncture from v.coccigea. There were used five types of vacutainers as follows: for the serum separation, with heparin, EDTA, citrate, and fluoride. Samples from each cow were taken in all five types of vacutainers respectively. There was determined the concentration of: albumin, total protein, glucose, Ca, P, BHB, NEFA, urea, cholesterol, triglycerides, ALT, AST, AP, GGT and bilirubin. The results of the analysis of samples that contained anticoagulants were compared statistically with analysis in serum that was considered to be the control one. It was also calculated the percentage of deviation in average values of concentration of metabolites obtained from samples with different anticoagulants in regard to the serum value. The results of the investigation show that anticoagulants influence the values of biochemical parameters in the blood of cows. In the samples where heparin was used as anticoagulant there was found higher albumin level (deviation 4.1%) as well as total protein (1.4%), but lower value of alkaline phosphatase activity relative to the serum (-33%). In the samples in which EDTA was used there were significantly lower levels of total protein (-5.8%), Ca (-49.6%), P (-17.7%), AP (-32%) and higher value AST (10.6%) compared to serum. In the samples where citrate or fluoride were used as anticoagulants there were found lower values of total protein, albumin, glucose (only citrate), Ca, P, BHB, NEFA, urea (only citrate), cholesterol, AP and GGT (only fluoride) and lower bilirubin when using citrate or higher when using fluoride, in comparison to the serum. Deviations of biochemical parameters measured from blood samples that were in citrate or fluoride were as follows: total protein (-5.3%, -5.2%), albumin (-7%, -5.7%), glucose (only citrate -6.2%), Ca (-55%, -82%), P (-29%, -24%), BHB (-53%, -80%), NEFA (-62.1%, -79 , 4%), urea (only citrate, -25.5%), cholesterol (-28.6%, -28.4%), AP (-38%, -32%), and GGT (only -17 fluoride, 8%) and higher bilirubin (-22.8%, 64.4%). For routine clinical biochemical analysis of blood of cows it is the most reliable to use the samples with heparin as an anticoagulant, because the use of other anticoagulants gives large variations in the values of biochemical parameters in relation to serum.

scholarly journals Serum and Saliva Concentrations of Biochemical Parameters in Men with Prostate Cancer and Benign Prostate Hyperplasia

2019 ◽  
Vol 51 (3) ◽  
pp. 243-251
Author(s):  
Hyder Farahani ◽  
Mona Alaee ◽  
Jamal Amri ◽  
Mahmoud-Reza Baghinia ◽  
Mohammad Rafiee
Keyword(s):  
Prostate Cancer ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Operating Characteristic ◽  
Specific Antigen ◽  
Correlation Coefficients ◽  
Diagnostic Efficacy ◽  
Prostate Hyperplasia ◽  
Benign Prostate ◽  
Control Study

Abstract Objectives To find suitable biomarkers for diagnosis of prostate cancer (PC) in serum and saliva; also, to evaluate the diagnostic efficacy of saliva in patients with PC. Methods This case-control study included 20 patients with PC and 20 patients with benign prostatic hyperplasia (BPH). Blood and saliva were collected from the participants and centrifuged. Serum and supernatant saliva were used for biochemical analysis. We evaluated serum and salivary levels of urea, creatinine, prostate-specific antigen (PSA), creatine kinase BB (CK-BB), zinc, β-2 microglobulin (B2M), and melatonin. Also, we used Mann-Whitney U testing, Spearman correlation coefficients, and receiver operating characteristic (ROC) analysis to evaluate the data. Results Serum and salivary concentrations of urea, creatinine, PSA, CK-BB, zinc, and B2M were significantly higher in patients with PC, compared with the BPH group (P &lt;.05). However, serum and salivary concentrations of melatonin were significantly lower in patients with PC, compared with BPH group (P &lt;.05). In both groups, salivary concentrations of all markers were lower (P &lt;.05), compared with those values in serum. We observed positive correlation between serum and salivary concentrations of all markers studied (P &lt;.05). Conclusion From the data, we conclude that investigation using saliva specimens is a noninvasive, simple, and effective tool for screening of biochemical parameters.

Old age, urinary obstruction and high lactate levels are risk factors to develop septic shock in urosepsis. A retrospective analysis.

2018 ◽  
Vol 17 (2) ◽  
pp. e1366
Author(s):  
S. Ferretti ◽  
E. Paiolo ◽  
F. Tagliaferri ◽  
E. Padua ◽  
T. Bocchialini ◽  
...  
Keyword(s):  
Risk Factors ◽  
Septic Shock ◽  
Retrospective Analysis ◽  
Old Age ◽  
Urinary Obstruction ◽  
Lactate Levels ◽  
High Lactate

scholarly journals Study of some biochemical parameters of vitiligo patients in Bangladesh

2012 ◽  
Vol 47 (2) ◽  
pp. 173-186
Author(s):  
Md Ziaul Amin ◽  
Mohd Hafizur Rahman ◽  
Mohammed A Satter
Keyword(s):  
Uric Acid ◽  
Blood Sugar ◽  
Total Protein ◽  
Biochemical Parameters ◽  
Biochemical Analysis ◽  
Age Groups ◽  
Age Group ◽  
Random Blood Sugar ◽  
Biochemical Studies ◽  
Average Serum

This study is focus on the biochemical analysis to investigate the condition of different biochemical parameters for the Vitiligo patients. This biochemical analysis deals with the blood serum level of random blood sugar, bilirubin, urea, total protein, SGOT, SGPT, alkaline phosphatase, total cholesterol and uric acids. The increasing some of the biochemical parameters were statistically significant with the age groups. Random blood sugar analysis of the 125 patient's age group 31- 40, 41- 50 and above 50 significantly higher than the control group. No significant changes were observed in serum bilirubin, serum urea, serum creatinine, serum alkaline phosphatase (ALP) and serum asparate amino transferase (AST) level in different age group of Vitiligo patients. In this study, the serum total proteins were slightly decreased in the age group patients below 10 and 11-20 as compared to healthy controls. The average serum total protein level of the below 10 and 11-20 age group patients were 5.31 g/dl and 6.50 g/dl respectively, whereas the controls were 5.95 g/dl and 6.92 g/dl respectively. Serum cholesterol, serum triglycerides and serum uric acid levels were significantly changed in the age group 11-20 and 31-40 compared with the healthy control. The average serum cholesterol level, triglyceride (TG) level and uric acid level were 152.17 mg/dl, 90.27 mg/dl, 4.75 mg/dl and 173.40 mg/dl, 149.95 mg/dl, 5.54 mg/dl respectively for the patients of 11-20 and 31-40 years age group. The controls of these parameters for the 11-20 years and 31-40 years age groups were 150.57 mg/dl, 94.41 mg/dl, 4.28 mg/dl and 156.75 mg/dl, 101mg/dl, 4.77mg/dl respectively. These biochemical studies on Vitiligo indicated that there was no significant changed observed in some biochemical parameters in the different age group of Vitiligo patients but some biochemical parameters like total protein, total cholesterol, triglycerides (TG), uric acid and random blood sugar level were significantly changed in different age group of Vitiligo patients specially among the 11-20 and 31-40 years age group. This biochemical studies would be helpful to know details about the pathological condition of the Vitiligo patients associated with other disease. DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11449 Bangladesh J. Sci. Ind. Res. 47(2), 173-186, 2012  

scholarly journals Biochemical composition of the hoof capsule of buffaloes and its influence on hoof quality

2017 ◽  
Vol 69 (1) ◽  
pp. 57-64 ◽  
Author(s):  
B.M. Assis ◽  
V.A.S. Vulcani ◽  
L.A.F. Silva ◽  
M. Dias ◽  
A. Pancotti ◽  
...  
Keyword(s):  
Organic Matter ◽  
Body Mass ◽  
Biochemical Composition ◽  
Biochemical Parameters ◽  
Crude Protein ◽  
Biochemical Analysis ◽  
Mineral Matter ◽  
Ether Extract ◽  
Dorsal Wall ◽  
Biochemical Analyses

ABSTRACT The purpose of this study was to establish the biochemical parameters of the abaxial wall, dorsal wall and sole of the hoof of the medial thoracic, lateral, and medial pelvic digits of buffalos. The hoof samples were subjected to destructive biochemical analyses to identify the dry material (DM), mineral matter (MM), organic matter (OM), crude protein (CP) and ether extract (EE) contents. Sulfur (S), calcium (Ca), potassium (K), phosphorus (P), zinc (Zn) and copper (Cu) levels were determined based on nondestructive biochemical analyses. The parameters of dry material, mineral matter, organic matter, crude protein and ether extract of hoof capsule of the digits of buffalos can be determined by means of both destructive and nondestructive biochemical analysis. In addition, this study revealed that the highest concentrations of DM, CP and minerals such as, K, Zn and Cu are concentrated in the digits that bear the greatest body mass weight, suggesting that there is a positive correlation between the aforementioned parameters and the strength and growth of the hoof capsule in the digits. As for the element S, this study demonstrated that its highest concentration is located in the lateral digits of the pelvic members.

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