Vinblastine and Prednisolone as Adjunctive Therapy for Canine Cutaneous Mast Cell Tumors

2004 ◽  
Vol 40 (2) ◽  
pp. 124-130 ◽  
Author(s):  
David R. Davies ◽  
Kenneth M. Wyatt ◽  
John E. Jardine ◽  
Ian D. Robertson ◽  
Peter J. Irwin
Keyword(s):  
Mast Cell ◽  
Local Recurrence ◽  
Adjunctive Therapy ◽  
Marginal Excision ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Residual Microscopic Disease ◽  
Related Mortality

Twenty-seven dogs with inadequately excised, cutaneous mast cell tumors (MCT; 20 residual microscopic disease, seven marginal excision) were treated with a vinblastine and prednisolone chemotherapeutic protocol. Twenty dogs were available for follow-up examination after 12 months. One dog suffered local recurrence of the tumor, four dogs developed new cutaneous tumors, and one dog had both events. Fourteen dogs were free of MCT. There was no confirmed tumor-related mortality. Although toxicity from the chemotherapy was generally mild, one dog died of sepsis during treatment.

Histopathology and Biologic Behavior of Pleomorphic Cutaneous Mast Cell Tumors in Fifteen Cats

2002 ◽  
Vol 39 (4) ◽  
pp. 452-457 ◽  
Author(s):  
T. O. Johnson ◽  
F. Y. Schulman ◽  
T. P. Lipscomb ◽  
L. D. Yantis
Keyword(s):  
Mast Cell ◽  
Prognostic Indicator ◽  
Giant Cells ◽  
Mitotic Rate ◽  
Surgical Biopsy ◽  
Mast Cell Tumors ◽  
Poorly Differentiated ◽  
Hematoxylin And Eosin ◽  
Cutaneous Mast Cell

Most feline cutaneous mast cell tumors (CMCT) are behaviorally benign; however, there is a subset of these tumors with marked pleomorphism (previously termed poorly differentiated) that have been reported to be more aggressive. In this study, pleomorphic CMCT from 15 cats were identified from surgical biopsy submissions, and follow-up clinical data were obtained for 14 of these cats. Pleomorphic CMCT were discrete dermal nodules composed of sheets of pleomorphic round cells. Tumors from all 15 cats contained markedly cytomegalic and karyomegalic cells; 9/15 tumors (60%) contained multinucleated tumor giant cells. Typical mast cell granules were easily identified in sections stained with hematoxylin and eosin and with metachromatic stains and based on ultrastructural evaluation in cytomegalic as well as smaller tumor cells, indicating that the tumors were not poorly differentiated. The mitotic rate was very low (<1 mitosis per 10 high-power fields [hpf]) in 14 of 15 tumors (93%). Affected cats were 6–19 years old (mean age = 11.5 years), and there was no breed or sex predilection. Two cats had local recurrence. The only cat that had a pleomorphic CMCT with a high mitotic rate (1–2 mitoses/hpf) subsequently developed numerous other dermal neoplasms and was euthanatized. In this study, the large majority of feline pleomorphic CMCT were behaviorally benign. Mitotic rate is likely an important prognostic indicator of CMCT behavior.

Iridium-192 Interstitial Brachytherapy as Adjunctive Treatment for Canine Cutaneous Mast Cell Tumors

2004 ◽  
Vol 40 (4) ◽  
pp. 309-315 ◽  
Author(s):  
Nicole C. Northrup ◽  
Royce E. Roberts ◽  
Todd W. Harrell ◽  
Karen L. Allen ◽  
Elizabeth W. Howerth ◽  
...  
Keyword(s):  
Mast Cell ◽  
Radiation Treatment ◽  
Interstitial Brachytherapy ◽  
Adjunctive Treatment ◽  
Mast Cell Tumors ◽  
Iridium 192 ◽  
Grade Ii ◽  
Cutaneous Mast Cell ◽  
Second Tumor

Eleven dogs with cutaneous mast cell tumors (MCTs) were treated with surgery and iridium-192 (192Ir) interstitial brachytherapy. Minimum tumor doses ranged from 47.2 to 63.3 Gy. Treated tumors were classified as grade II (n=7) or III (n=4). Five dogs had recurrences with a median progression-free interval of 1391 days, and six dogs had no recurrence at a median follow-up time of 942 days. Acute adverse effects were well tolerated, and late effects were mild. One dog developed a second tumor of a different cell type in the radiation treatment field.

scholarly journals Stereology in Grading and Prognosis of Canine Cutaneous Mast Cell Tumors

2021 ◽  
pp. 030098582098513
Author(s):  
Mafalda Casanova ◽  
Sandra Branco ◽  
Inês Berenguer Veiga ◽  
André Barros ◽  
Pedro Faísca
Keyword(s):  
Mast Cell ◽  
Clinical Outcome ◽  
Prognostic Value ◽  
Histological Grade ◽  
Intraobserver Variability ◽  
Low Grade ◽  
High Grade ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Grade Iii

Canine cutaneous mast cell tumors (ccMCTs) are currently graded according to Patnaik and Kiupel grading schemes. The qualitative and semiquantitative parameters applied in these schemes may lead to inter- and intraobserver variability. This study investigates the prognostic value of volume-weighted mean nuclear volume ([Formula: see text]), a stereological estimation that provides information about nuclear size and its variability. [Formula: see text] of 55 ccMCTs was estimated using the “point-sampled intercept” method and compared with histological grade and clinical outcome. The clinical history of dogs treated with surgical excision alone was available for 30 ccMCTs. Statistical differences in [Formula: see text] were found between grade II ([Formula: see text]= 115 ± 29 µm3) and grade III ccMCTs ([Formula: see text]= 197 ± 63 µm3), as well as between low-grade ([Formula: see text]= 113 ± 28 µm3) and high-grade ccMCTs ([Formula: see text]= 184 ± 63 µm3). An optimal cutoff value of [Formula: see text] ≥ 150 µm3 and [Formula: see text] ≥ 140 µm3 was determined for grade III and high-grade ccMCTs, respectively. In terms of prognosis, [Formula: see text] was not able to predict the clinical outcome in 42% of the cases; however, cases with [Formula: see text]<125 µm3 had a favorable outcome. These results indicate that, despite having limited prognostic value when used as a solitary parameter, [Formula: see text] is highly reproducible and is associated with histological grade as well as with benign behavior.

scholarly journals Ancillary techniques on the evaluation of canine cutaneous mast cell tumors from Brazil

2016 ◽  
Vol 46 (10) ◽  
pp. 1804-1810 ◽  
Author(s):  
Mariana Martins Flores ◽  
Renata Dalcol Mazaro ◽  
Ingeborg Maria Langohr ◽  
Alma Roy ◽  
Keith Strother ◽  
...  
Keyword(s):  
Mast Cell ◽  
Pcr Amplification ◽  
The United States ◽  
Growth Fraction ◽  
Grading System ◽  
Low Grade ◽  
Ki 67 ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell ◽  
Exon 11

ABSTRACT: The use of histologic classification by a 2-tier grading system only, immunohistochemistry (IHC) for KIT and Ki-67 and polymerase chain reaction (PCR) for internal tandem duplications (ITD) on exon 11 has improved the prognostication of canine cutaneous mast cell tumors (CCMTs) particularly in the United States. However, these techniques are not commonly used in most Brazilian laboratories. Likewise, no studies, to date, have investigated the occurrence of ITD in CCMTs from the country. Thus, this study tested the 2-tier grading system, the immunohistochemistry for KIT and Ki-67 and the PCR for exon 11 in a group of Brazilian CCMTs with the goal of investigating the applicability of these tests in a Brazilian laboratory. Of the 39 CCMTs, 69.2% (27/39) were identified as low-grade and 30.8% (12/39) as high-grade by a 2-tier grading system. All tumors had a KIT expression pattern II, and 30.6% (11/36) had a high growth fraction (Ki-67). PCR amplification was successful in four of the 11 tumors examined. Two of these (50%) were positive for ITD. This study highlights the importance of using auxiliary techniques in the CCMT evaluation, identifies limitations and confirms the applicability of these methods on a routine diagnostic basis in Brazil. Our results will help to improve the prognostication of CCMTs in Brazilian diagnostic laboratories, encouraging the use of supplementary methods.

Variability in tumor margin reporting for soft tissue sarcoma and cutaneous mast cell tumors in dogs: A systematic review

2020 ◽  
Author(s):  
Brittany E. Abrams ◽  
Allison B. Putterman ◽  
Audrey Ruple ◽  
Vincent Wavreille ◽  
Laura E. Selmic
Keyword(s):  
Systematic Review ◽  
Mast Cell ◽  
Soft Tissue ◽  
Soft Tissue Sarcoma ◽  
Tumor Margin ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell

Expression of Proto-Oncogene C-Kit and Correlation with Morphological Evaluations in Canine Cutaneous Mast Cell Tumors

2008 ◽  
Vol 30 (3) ◽  
pp. 609-621 ◽  
Author(s):  
Letizia Passantino ◽  
Giuseppe Passantino ◽  
Attilio Cianciotta ◽  
Maria Rosaria Ribaud ◽  
Giuseppe Lo Presti ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell

scholarly journals Microvessel density is as a prognostic factor in canine cutaneous mast cell tumors

2013 ◽  
Vol 7 (Suppl 2) ◽  
pp. P62
Author(s):  
Daniella FL Barbosa ◽  
Lidia H Pulz ◽  
Nilton P Santos ◽  
Silvia Kleeb ◽  
José Xavier ◽  
...  
Keyword(s):  
Mast Cell ◽  
Prognostic Factor ◽  
Microvessel Density ◽  
Mast Cell Tumors ◽  
Cutaneous Mast Cell

scholarly journals The Relevance of CD117-Immunocytochemistry Staining Patterns to Mutational Exon-11 inc-kitDetected by PCR from Fine-Needle Aspirated Canine Mast Cell Tumor Cells

2014 ◽  
Vol 2014 ◽  
pp. 1-8 ◽  
Author(s):  
A. Sailasuta ◽  
D. Ketpun ◽  
P. Piyaviriyakul ◽  
S. Theerawatanasirikul ◽  
P. Theewasutrakul ◽  
...  
Keyword(s):  
Mast Cell ◽  
Needle Aspiration ◽  
Biological Behavior ◽  
High Grade ◽  
Fine Needle ◽  
Mast Cell Tumors ◽  
Diagnosis And Prognosis ◽  
Cutaneous Mast Cell ◽  
Exon 11 ◽  
Two Parameters

Canine cutaneous mast cell tumors (MCT) are the lethal skin tumors. The biological behavior of the MCT cells is quite varied and unpredictable. Almost MCT dogs usually require a rapid diagnosis and therapy. However, MCT diagnosis and prognosis are still dependent on histopathology which is rather inconvenient, time-consuming, painful, and harmful for some cases. Indeed, MCT can be easily accessible using fine-needle aspiration (FNA). In this study, our biopsy specimens were classified as low- and high-grade MCT based on the novel 2-tier histopathologic grading system. We have demonstrated the usage of fine-needle aspirated MCT cells (FNA-MCT cells) from these specimens as a primary cell source to study the distribution of CD117-immunocytochemistry (CD117-ICC) staining patterns and the frequency of internal tandem duplication- (ITD-) mutant exon-11 ofc-kit. The result has substantially shown that there were three staining patterns identified in the cells. Only paranuclear pattern was significantly increased in the cells from high-grade MCT. Altogether, the ITD-mutant exon-11 was also detectable only in these cells. Therefore, the result has supported our hypothesis that there was an increased opportunity to observe a higher CD117-ICC staining pattern and exon-11 mutation in high-grade MCT; even these two parameters may not precisely indicate a histopathological grade.

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